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11.
Chakrabarty S Das A Bhattacharya A Chakrabarti G 《Journal of agricultural and food chemistry》2011,59(5):2040-2048
Here we studied the antiproliferative activity of theaflavins in cervical carcinoma HeLa cells by investigating their effects on cellular microtubules and purified goat brain tubulin. Theaflavins inhibited proliferation of HeLa cells with IC(50) value of 110 ± 2.1 μg/mL (p = < 0.01), caused cell cycle arrest at G(2)/M phase and induced apoptosis with alteration of expression of pro- and antiapoptotic proteins. Along with these antiproliferative activities, theaflavins act as microtubule depolymerizers. Theaflavins disrupted the microtubule network accompanied by alteration of cellular morphology and also decreased the polymeric tubulin mass of the cells. The polymerization of cold treated depolymerized microtubules in HeLa cells was prevented in the presence of theaflavins. In vitro polymerization of purified tubulin into microtubules was also inhibited by theaflavins with an IC(50) value of 78 ± 2.43 μg/mL (P < 0.01). Thus, disruption of cellular microtubule network of HeLa cells through microtubule depolymerization may be one of the possible mechanisms of antiproliferative activity of theaflavins. 相似文献
12.
Bhattacharyya J Banerjee H Bhattacharyya A 《Journal of agricultural and food chemistry》2003,51(14):4013-4016
Fenazaquin (I) is a new acaricide of the quinazoline class. The photodecomposition of I was studied in aqueous methanolic and 2-propanolic solution under UV light (30 h) and sunlight (70 h) separately. The photolytic half-lives in aqueous methanolic solution were found to be 17.1 h (UV) and 38.1 h (sunlight), whereas these were 12.9 h (UV) and 29.2 h (sunlight) for aqueous 2-propanolic solution; all followed a first-order reaction kinetics. Six photoproducts were obtained: beta-phenyl (p-tert-butyl) ethyl alcohol (II), 4-hydroxyquinazoline (III), p-tert-butyl vinyl benzene (IV), 2,4-dihydroxyquinazoline (V), phenyl (p-tert-butyl) acetic acid (VI), and 2-methyl-2-[4'-(2' '-hydroxyethyl)phenyl]propanoic acid (VII). Compounds VI and VII could be isolated only from aqueous 2-propanolic solution under sunlight irradiation. The major degradation products are formed as a result of cleavage of the ether bridge linking the quinazoline and phenyl ring systems of the molecule, oxidation of the tert-butyl substituent, and oxidation of the heterocyclic portion of the quinazoline ring. A probable mechanism of formation of the photoproducts is also suggested. 相似文献
13.
Bhattacharya G 《Science (New York, N.Y.)》1955,121(3143):427
In the article "On the protection against alloxan diabetes by hexoses" by G. Bhattacharya in the 19 Nov. 1954 issue, page 842, the heading for the second column of Table 2 should read "Oxygen uptake (microlit O(2)/30 min)." 相似文献
14.
Price DC Chan CX Yoon HS Yang EC Qiu H Weber AP Schwacke R Gross J Blouin NA Lane C Reyes-Prieto A Durnford DG Neilson JA Lang BF Burger G Steiner JM Löffelhardt W Meuser JE Posewitz MC Ball S Arias MC Henrissat B Coutinho PM Rensing SA Symeonidi A Doddapaneni H Green BR Rajah VD Boore J Bhattacharya D 《Science (New York, N.Y.)》2012,335(6070):843-847
15.
16.
Ramesh Chandra Malick Asit Kumar Bera Hemanta Chowdhury Manojit Bhattacharya Tanuja Abdulla Himanshu Sekhar Swain Raju Baitha Vikas Kumar Basanta Kumar Das 《Aquaculture Research》2020,51(6):2410-2420
The disease outbreaks in aquaculture system of wetlands are the major cause of fish mortality. Among various bacterial septicaemic diseases, fish mortality caused by Acinetobacter spp. is recently reported in different fish species. Fish disease outbreak was investigated in a wetland of West Bengal, India to identify the aetiological factors involved. The moribund fish were examined and subjected to bacterial isolation. Two bacterial causative agents were identified as Acinetobacter junii and Acinetobacter pittii by biochemical characterization and 16S rRNA gene amplification. Both the isolates were oxidase‐negative, nitrate‐negative, catalase‐positive and indole‐negative. The molecular identification using 16S rRNA gene sequencing and phylogenetic tree analysis further confirmed the two Acinetobacter spp. with 97%–99% similarity. The antibiotic resistance patterns of these two bacteria revealed that both of them were resistant to β‐lactam, cefalexin, cephalothin, amoxyclav, cefuroxime, cefadroxil, clindamycin, vancomycin and penicillin. In addition, A. pittii was also resistant to other antibiotics of cephams group such as ceftazidime and cefotaxime. In the challenge experiment, both A. junii and A. pittii were found to be pathogenic with LD50 of 1.24 × 105 and 1.88 × 107 cfu/fish respectively. Histopathological examination of gill, liver and kidney revealed prominent changes supporting bacterial septicaemia. The investigation reports for the first time on concurrent infection by A. junii and multidrug‐resistant (MDR)‐A. pittii as emerging fish pathogens to cause severe mortality in Labeo catla and Hypophthalmichthys molitrix in a freshwater wetland. 相似文献
17.
Successful reproduction is considered to be the major determining factor for a weedy population to establish, colonize and infest. The breeding systems and seed production of six weedy taxa of Bidens were studied at an experimental garden in Jiangsu province in China. The results from four treatments (open pollination, bagged capitulum, decapitated capitulum, and pollen supplement) indicated that, with the exception of Bidens pilosa var. radiata, all the other five Bidens taxa; namely, B. pilosa var. pilosa, B. pilosa var. minor, B. frondosa, B.bipinnata, and B. biternata, were self‐fertile with a high seed‐set in bagged capitula, and a high autofertility index. Including the trait of vegetative propagation in self‐incompatible B. pilosa var. radiata, all the six weedy taxa of Bidens had the capacity of uniparental reproduction. The pollen limitation values for all six Bidens taxa were low in the present study. However, the seed production of B. pilosa var. radiata is likely to decrease in the absence of sufficient pollinators, related to its high pollen contribution and potential pollen limitation indices. The seed production per plant and per capitulum of the three varieties of B. pilosa was the highest among the six taxa and might contribute to their serious weediness. The breeding system type and seed number per capitulum could be used as a classification reference for Bidens taxa. 相似文献
18.
Seroprevalence of brucellosis in yaks (Poephagus grunniens) in India and evaluation of protective immunity to S19 vaccine 总被引:1,自引:1,他引:0
Bandyopadhyay S Sasmal D Dutta TK Ghosh MK Sarkar M Sasmal NK Bhattacharya M 《Tropical animal health and production》2009,41(4):587-592
The present study was carried out to explore the seroprevalence of brucellosis in yaks of North-Eastern hilly yak tracts of
Arunachal Pradesh, India. Of 374 animals tested, 23.79, 21.11 and 18.98% were found positive for brucellosis using avidin-biotin
ELISA (AB-ELISA), Rose-Bengal plate test (RBPT) and standard tube-agglutination test (STAT), respectively. The relative sensitivity
and specificity for STAT were 79.77 and 100%, respectively and the same for RBPT were 88.76 and 100%, respectively in comparison
to AB-ELISA. The alarming prevalence as recorded was highest among the yak cows (31.42%) followed by heifers (23.85%) and
bulls (8.88%). The immune response in yaks following standard dose of calfhood vaccination with Brucella abortus strain 19 vaccine showed that protective antibody level persisted up to 210 days. This is the first report from India on
prevalence of brucellosis and immunization with B abortus strain 19 vaccine in yaks. The present investigation would be a valuable guideline for future control measure and eradication
programme of brucellosis in yaks. 相似文献
19.
The nontarget effects of three plant growth stimulants (gibberellic acid, Siapton and triacontanol) and two plant growth retardants
(chlormequat chloride and mepiquat chloride) on the survivorship and developmental parameters of Spilarctia obliqua Walker (Lepidoptera: Arctiidae) were studied. For each compound, various concentrations (0.125×, 0.25×, 0.5×, × , 2× and
4×; × = labeled dose) bracketing the labeled dose were prepared by serial dilution and mixed with an artificial diet for the
insect. Gibberellic acid caused an increase in the larval period at × − 4×, but no marked difference was observed in the pupal
period or in larval survival at any concentration. Siapton induced a significant increase in larval period at the two highest
doses but caused significant reduction in pest survival at × − 4×. Interestingly, triacontanol induced a significant reduction
in pest survivorship at almost all doses. The LC50 for triacontanol was 0.206% and LT50 was 266.5 hours at the labeled dose. However, triacontanol did not cause any significant difference in larval or pupal periods
at any dose tested. Chlormequat chloride and mepiquat chloride caused significant reduction of larval survivorship at concentrations
higher than the labeled doses, and no effect on the larval period. Intriguingly, a significant reduction in pupal period was
observed with chlormequat chloride at × and 0.25×. Based on the marked effect of triacontanol on pest survivorship, this study
suggests incorporating triacontanol in integrated pest management modules for S. obliqua. Caution in the use of gibberellic acid and Siapton for pest infestations is also indicated. 相似文献
20.
Moumita Gangopadhyay Saikat DewanjeeDipjyoti Chakraborty Sabita Bhattacharya 《Industrial Crops and Products》2011,33(2):445-450
The present study describes the role of different exogenous hormones on morphology and plumbagin production in Plumbago indica hairy roots. It was also aimed to conserve elite root clones via synthetic seed technology. Insertion of rolB gene in transformed roots was confirmed by polymerase chain reaction followed by southern blot analysis. Hairy roots were treated with single or in combination of different phytohormones viz. IAA, IBA, 2, 4-D, NAA, BAP, GA3 and ABA. Cultures incubated with GA3 (0.5 mg l−1) yielded highest root growth due to formation of profuse lateral branching while NAA (0.5 mg l−1) treatment caused highest plumbagin accumulation. Cultures incubated with 2, 4-D exhibited highest inhibitory effect in terms of both root growth and plumbagin production. All phytohormones were found to be effective at lower concentration. In combinatorial study, GA3 + NAA (0.5 mg l−1, each) was found optimum for root biomass and plumbagin production at earlier stage of culture. Different combinations of auxins and BAP induced different morphologies ranging from reduction of lateral branching to rapid disorganization of root matrix. The combinations of ABA and selected auxins were not found promising at any of selected concentration. Based on the effect of exogenous hormones on hairy root culture, elite root clones were selected and encapsulated with sodium alginate matrix. Uniform shaped alginate coated synthetic seeds were conserved up to 6 months exhibited high regeneration potential without disturbing plumbagin content. 相似文献