First report and molecular characterization of Trypanosoma vivax in cattle from state of Pernambuco, Brazil     

Pimentel Dde S  Ramos CA  Ramos RA  de Araújo FR  Borba ML  Faustino MA  Alves LC 《Veterinary parasitology》2012,185(2-4):286-289

This paper describes an outbreak of Trypanosoma vivax for the first time in the state of Pernambuco, Brazil, affecting dairy cattle in the municipality of Itambé in the northern coastal zone of the state. Clinical signs compatible with infection by blood protozoa and epidemic miscarriages were observed. The diagnosis of T. vivax was confirmed through biometric microscopy and molecular analysis with PCR and DNA sequencing. The T. vivax isolate detected in the present study proved to be genetically very close to other Brazilian isolates of the protozoan despite being geographically distant.  相似文献   

Open surgical correction combined with an external splint for correction of a non-compliant pectus excavatum in a cat     

Mestrinho LA  Ferreira CA  Lopes AM  Niza MM  Hamaide AJ 《Journal of Feline Medicine and Surgery》2012,14(2):151-154

A 4-month-old domestic shorthair female cat weighing 1.3 kg was presented for evaluation of respiratory distress. The animal showed evident dyspnoea with exercise intolerance and a marked concave deformation of the sternum. After measurements of the fronto-sagittal and vertebral indexes, the pectus was classified as moderate and surgery was elected. Surgical correction was performed using an open approach to the sternum with osteotomy of the last sternebra and costochondral junctions of the eighth and ninth ribs bilaterally. A silicone based, U-shape external splint was manufactured and used to stabilise the sternum. Immediate and 5-week postsurgical radiographs revealed a decreased concavity of the sternum and an increase thoracic height at the level of the last sternebra. Postoperative results suggest that this technique could be an effective and economical option for cats with pectus excavatum with a non-compliant sternum.  相似文献   

Multiple strains of Mycobacterium bovis revealed by molecular typing in a herd of cattle     

Figueiredo EE  Ramos DF  Medeiros L  Silvestre FG  Lilenbaum W  Silva JT  Paschoalin VM  Dellagostin OA 《Veterinary journal (London, England : 1997)》2012,193(1):296-298

Mycobacterium bovis isolates from an outbreak of bovine tuberculosis in a herd of cattle in Rio de Janeiro, Brazil, were analysed by spoligotyping and variable-number tandem repeat PCR analysis of the mycobacterial interspersed repetitive unit and exact tandem repeats. Molecular typing revealed a high genetic diversity of strains in the herd. The genetic diversity could be explained by the introduction of infected animals from different sources.  相似文献   

Xanthorhodopsin: a proton pump with a light-harvesting carotenoid antenna     

Balashov SP  Imasheva ES  Boichenko VA  Antón J  Wang JM  Lanyi JK 《Science (New York, N.Y.)》2005,309(5743):2061-2064

Energy transfer from light-harvesting carotenoids to chlorophyll is common in photosynthesis, but such antenna pigments have not been observed in retinal-based ion pumps and photoreceptors. Here we describe xanthorhodopsin, a proton-pumping retinal protein/carotenoid complex in the eubacterium Salinibacter ruber. The wavelength dependence of the rate of pumping and difference absorption spectra measured under a variety of conditions indicate that this protein contains two chromophores, retinal and the carotenoid salinixanthin, in a molar ratio of about 1:1. The two chromophores interact strongly, and light energy absorbed by the carotenoid is transferred to the retinal with a quantum efficiency of approximately 40%. The antenna carotenoid extends the wavelength range of the collection of light for uphill transmembrane proton transport.  相似文献   

Cercosporiosis resistance in coffee germplasm collection     

Deila M. dos S. Botelho  Mário L. V. de Resende  Vinícius T. Andrade  Antônio A. Pereira  Flavia R. A. Patricio  Pedro M. R. Junior  Claudio Ogoshi  Juliana C. de Rezende 《Euphytica》2017,213(6):117

Cercosporiosis, or brown eye spot, is currently one of the main diseases of the coffee tree. It is caused by Cercospora coffeicola Berk. & Cooke. Nevertheless, genetic resistance to this disease has not yet been explored in any depth. Our objectives (a) were evaluate the response of 124 accessions from the germplasm collection of the Minas Gerais State (GC), Brazil, and eight commercial cultivars of C. arabica to cercosporiosis and (b) determine the best way to perform early progenies selections via controlled greenhouse experiments. Three controlled greenhouse experiments (1–3) were run in different seasons to determine the best way to proceed the selection. The seedlings were inoculated with a four isolates mixture obtained from different regions. The experimental data were analyzed individually (1–3), in a joint analysis, and as repetitions of a randomized complete block design. In each analysis we estimated genetic parameters and E-BLUP (empirical best linear unbiased predictor) genotypic values of the access. There was genetic variability to C. coffeicola resistance among the coffee tree accessions germplasm collection. Therefore, genetic improvements could be obtained by selection. Experimental repetitions in different seasons increase the selection efficiency and reliability of resistant genotypes with low cercosporiosis severity. The genotype Sarchimor MG 8840 showed the highest resistance level followed by Guatenano and the Timor Hybrid UFV 377-34, Timor Hybrid UFV 376-14 BE 5, and Wush–Wush × Timor Hybrid UFV 366-08.  相似文献   

Characterization of lignin in heartwood,sapwood and bark from Tectona grandis using Py–GC–MS/FID     

Ana Lourenço  Duarte Miranda Neiva  Jorge Gominho  António Velez Marques  Helena Pereira 《Wood Science and Technology》2015,49(1):159-175

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Seasonal occurrence of Piezodorus guildinii on different plants including morphological and physiological changes     

Maria S. Zerbino  Nora A. Altier  Antônio R. Panizzi 《Journal of pest science》2015,88(3):495-505

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Performance of the Santa Ines breed raised on pasture in semiarid tropical regions and factors that explain trait variation     

Adriana de Farias Jucá  Juliana Cantos Faveri  Geraldo Magalhães Melo Filho  Antônio de Lisboa Ribeiro Filho  Hymerson Costa Azevedo  Evandro Neves Muniz  Luís Fernando Batista Pinto 《Tropical animal health and production》2014,46(7):1249-1256

This study aimed to evaluate sex, the number of lambs per birth, and the family effects on production traits in the Santa Ines breed of sheep by estimating the least square means and coefficient of variance for those traits. A total of 484 lambs were evaluated for the following traits: weight at birth, at weaning, and at 240 days of age; weight gain during the pre-weaning and post-weaning periods; height, width, and length of different body regions; and rib eye area and fat thickness between the 12th and 13th ribs. We observed coefficients of variation higher than 10 % for several traits. Generally, males were larger than females (P?P?P?ANOVA.  相似文献   

Immunosuppression abrogates resistance of young rabbits to Rabbit Haemorrhagic Disease (RHD)     

Raquel M Marques  Luzia Teixeira  Artur P águas  Joana C Ribeiro  António Costa-e-Silva  Paula G Ferreira 《Veterinary research》2014,45(1):14

Rabbit Haemorrhagic Disease (RHD) is caused by a calicivirus (RHDV) that kills 90% of infected adult European rabbits within 3 days. Remarkably, young rabbits are resistant to RHD. We induced immunosuppression in young rabbits by treatment with methylprednisolone acetate (MPA) and challenged the animals with RHDV by intramuscular injection. All of these young rabbits died within 3 days of infection due to fulminant hepatitis, presenting a large number of RHDV-positive dead or apoptotic hepatocytes, and a significant seric increase in cytokines, features that are similar to those of naïve adult rabbits infected by RHDV. We conclude that MPA-induced immunosuppression abrogates the resistance of young rabbits to RHD, indicating that there are differences in the innate immune system between young and adult rabbits that contribute to their distinct resistance/susceptibility to RHDV infection.  相似文献   

Mucoadhesive chitosan films as a potential ocular delivery system for ofloxacin: preliminary in vitro studies     

Gustavo de Oliveira Fulgêncio  Fernando Antônio B. Viana  Rodrigo Otávio S. Silva  Francisco Carlos F. Lobato  Raul R. Ribeiro  Juçara R. Fanca  Ricardo Martins D. Byrro  André Augusto G. Faraco  Armando da Silva Cunha‐Júnior 《Veterinary ophthalmology》2014,17(2):150-155

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