Amazonian deforestation models     

Câmara G  Dutra Aguiar AP  Escada MI  Amaral S  Carneiro T  Vieira Monteiro AM  Araújo R  Vieira I  Becker B 《Science (New York, N.Y.)》2005,307(5712):1043-4; author reply 1043-4

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Pyogenic bacterial infections in humans with MyD88 deficiency     

von Bernuth H  Picard C  Jin Z  Pankla R  Xiao H  Ku CL  Chrabieh M  Mustapha IB  Ghandil P  Camcioglu Y  Vasconcelos J  Sirvent N  Guedes M  Vitor AB  Herrero-Mata MJ  Aróstegui JI  Rodrigo C  Alsina L  Ruiz-Ortiz E  Juan M  Fortuny C  Yagüe J  Antón J  Pascal M  Chang HH  Janniere L  Rose Y  Garty BZ  Chapel H  Issekutz A  Maródi L  Rodriguez-Gallego C  Banchereau J  Abel L  Li X  Chaussabel D  Puel A  Casanova JL 《Science (New York, N.Y.)》2008,321(5889):691-696

MyD88 is a key downstream adapter for most Toll-like receptors (TLRs) and interleukin-1 receptors (IL-1Rs). MyD88 deficiency in mice leads to susceptibility to a broad range of pathogens in experimental settings of infection. We describe a distinct situation in a natural setting of human infection. Nine children with autosomal recessive MyD88 deficiency suffered from life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease. However, these patients were otherwise healthy, with normal resistance to other microbes. Their clinical status improved with age, but not due to any cellular leakiness in MyD88 deficiency. The MyD88-dependent TLRs and IL-1Rs are therefore essential for protective immunity to a small number of pyogenic bacteria, but redundant for host defense to most natural infections.  相似文献   

Partially replacing sorghum silage with cactus (Opuntia stricta) cladodes in a soybean oil-supplemented diet markedly increases trans-11 18:1, cis-9, trans-11 CLA and 18:2 n-6 contents in cow milk     

Marco A. S. Gama  Talita A. de Paula  Antônia S. C. Véras  Sebastião I. Guido  Cristiano A. V. Borges  Rosemar Antoniassi  Fernando C. F. Lopes  Maria L. M. W. Neves  Marcelo de A. Ferreira 《Journal of animal physiology and animal nutrition》2021,105(2):232-246

Based on low 18:0 contents observed in milk fat of cows fed cactus cladodes (CC), we hypothesized that including Opuntia stricta cladodes in a soybean oil (SO)-supplemented diet would promote incomplete rumen biohydrogenation of supplemental PUFA, leading to increased trans-11 18:1 and cis-9, trans-11 CLA contents in milk. Twelve Holstein cows were used in a two-period study: (a) Baseline: all cows received a total mixed ration (TMR) composed of sorghum silage (SS) and a concentrate containing no SO for 14 days; (b) Treatment: cows received one of the following SO-supplemented diets for 21 days: (1) SS-TMR: a TMR composed of SS and a SO-enriched concentrate, (2) CC-TMR: a TMR containing CC as a partial substitute for SS plus the SO-enriched concentrate, and (3) CC-PMR: same diet as in treatment 2, but CC were mixed with the SO-enriched concentrate and fed as a partial mixed ration (PMR). Both CC diets increased relative abundances of trans-11 18:1, cis-9, trans-11 CLA, and 18:2 n-6 in milk fat, whereas opposite effects were observed on 18:0 and cis-9 18:1. Proportion of 18:2 n-6 increased, and cis-9, trans-11 CLA tended to increase with CC-PMR as compared to CC-TMR, whereas 18:3 n-3 was higher with CC-PMR than with SS-TMR. Proportions of several odd- and branched-chain fatty acids, certain 18:1 isomers, and trans-9, cis-11 CLA changed with CC diets, notably with CC-PMR. Milk yield and intake of most nutrients (except fibre) increased or tended to increase with the CC diets, whereas gross milk composition was unaltered. Stearoyl-CoA desaturase-1 index for C18 (SCD₁₈) was higher with CC-PMR than with SS-TMR, and milk n-6:n-3 FA ratio and apparent transfer of 18:2 n-6 to milk increased with CC diets. These results indicate that Opuntia stricta cladodes can be a valuable feed ingredient for improving the nutraceutical value of milk fat.  相似文献   

Effect of dietary inclusion of Spirulina on production performance,nutrient digestibility and meat quality traits in post-weaning piglets     

Cátia Falcão Martins  José Pestana Assunção  David M. Ribeiro Santos  Marta Sofia Morgado dos S. Madeira  Cristina Maria Riscado Pereira Mateus Alfaia  Paula Alexandra Antunes Brás Lopes  Diogo Francisco Maurício Coelho  José Pedro Cardoso Lemos  André M. de Almeida  José António Mestre Prates  João Pedro Bengala Freire 《Journal of animal physiology and animal nutrition》2021,105(2):247-259

The effect of Spirulina (Arthrospira platensis), individually or in combination with two commercial carbohydrases, in piglet diets was assessed on growth performance, nutrient digestibility and meat quality traits. Forty post-weaned male piglets from Large White × Landrace sows crossed with Pietrain boars with an initial live weight of 12.0 ± 0.89 kg were used. Piglets were assigned to one of four dietary treatments (n = 10): cereal and soya bean meal base diet (control), base diet with 10% Spirulina (SP), SP diet supplemented with 0.005% Rovabio^® Excel AP (SP + R) and SP diet supplemented with 0.01% lysozyme (SP + L). Animals were slaughtered after a 4-week experimental period. Growth performance was negatively affected by the incorporation of Spirulina in the diets, with an average decrease of 9.1% on final weight, in comparison with control animals. Total tract apparent digestibility (TTAD) of crude protein was higher (p < .05) in the control group than in other groups. In addition, lysozyme increased TTAD of crude fat and acid detergent fibre, relative to the SP and control groups, respectively. In addition, the incorporation of Spirulina, individually and supplemented with enzymes, did not impair meat quality traits. Surprisingly, no protective effect against lipid oxidation was observed with the inclusion of Spirulina in pork after 7 days of storage. This study indicates that growth performance of post-weaning piglets was impaired by the incorporation of 10% Spirulina in the diets, which is mediated by an increase in digesta viscosity and a lower protein digestibility, as a consequence of the resistance of microalga proteins to the action of endogenous peptidases. In addition, it also indicates that lysozyme, in contrast to Rovabio^® Excel AP, is efficient in the degradation of Spirulina cell wall in piglet's intestine. However, the digestion of proteins liberated by Spirulina cell wall disruption is still a challenge.  相似文献   

Proteomic profile of pre-implantational ovine embryos produced in vivo     

Deisy J. D. Sanchez  Fabio R. Vasconcelos  Antônio C. A. Teles-Filho  Arabela G. A. Viana  Aline M. A. Martins  Marcelo V. Sousa  Mariana S. Castro  Carlos A. Ricart  Wagner Fontes  Marcelo Bertolini  Ivan C. Bustamante-Filho  Arlindo A. Moura 《Reproduction in domestic animals》2021,56(4):586-603

The present study was conducted to decipher the proteome of in vivo-produced pre-implantation ovine embryos. Ten locally adapted Morana Nova ewes received hormonal treatment and were inseminated 12 hr after ovulation. Six days later, 54 embryos (morula and blastocyst developmental state) were recovered from eight ewes and pooled to obtain sufficient protein for proteomic analysis. Extracted embryo proteins were analysed by LC-MS/MS, followed by identification based on four database searches (PEAKS, Proteome Discoverer software, SearchGUI software, PepExplorer). Identified proteins were analysed for gene ontology terms, protein clusters and interactions. Genes associated with the ovine embryo proteome were screened for miRNA targets using data sets of TargetScan ( http://www.targetscan.org ) and mIRBase ( http://www.mirbase.org ) servers. There were 667 proteins identified in the ovine embryos. Biological processes of such proteins were mainly related to cellular process and regulation, and molecular functions, to binding and catalytic activity. Analysis of the embryo proteins revealed 49 enriched functional clusters, linked to energy metabolism (TCA cycle, pyruvate and glycolysis metabolism), zona pellucida (ZP), MAPK signalling pathway, tight junction, binding of sperm to ZP, translation, proteasome, cell cycle and calcium/phospholipid binding. Sixteen miRNAs were related to 25 pre-implantation ovine embryo genes, all conserved in human, bovine and ovine species. The interaction network generated by miRNet showed four key miRNAs (hsa-mir-106b-5p; hsa-mir-30-5p; hsa-mir-103a-5p and hsa-mir-106a-5p) with potential interactions with embryo-expressed genes. Functional analysis of the network indicated that miRNAs modulate genes related to cell cycle, regulation of stem cell and embryonic cell differentiation, among others. Retrieved miRNAs also modulate the expression of genes involved in cell signalling pathways, such as MAPK, Wnt, TGF-beta, p53 and Toll-like receptor. The current study describes the first major proteomic profile of 6-day-old ovine embryos produced in vivo, setting a comprehensive foundation for our understanding of embryo physiology in the ovine species.  相似文献   

Testicular shape,scrotal skin thickness and testicular artery blood flow changes in bulls of different ages     

Luís Afonso Marques Claus  Flávio Antônio Barca Junior  Celso Koetz Junior  Gabriel Ribas Pereira  Patrícia da Cruz Fávaro  Fabio Procópio Ferreira  Victor Hugo Gonçalves Galdioli  Marcelo Marcondes Seneda  Edson Luís de Azambuja Ribeiro 《Reproduction in domestic animals》2021,56(7):1034-1039

The aim of this study was to compare the biometric testicular characteristics, skin thickness and haemodynamics of the testicular artery of 12- and 24-month-old bulls using Doppler ultrasonography, the study was conducted using 48 indicus-taurus animals. The scrotal circumference (SC) and biometry characteristics of the bulls were measured to calculate the testicular volume. Doppler ultrasonography was used to obtain the haemodynamic values of the testicular artery. The skin thickness and volume were lower (p<.01) in the younger bulls (12 months:4.68 ± 0.68 mm; 168.76 ± 47.96 cm³) versus 24 months (5.05 ± 0.89; 499.73 ± 129.24 cm³) animals (p<.01). During diastole, mean velocity was lower in the 12 months (7.98 ± 3.83) than in the 24 months (11.37 ± 4.15) animals (p <.05). The 12-month-old animals had higher pulsatility and resistivity indices (0.49 ± 0.02; 0.51 ± 0.20) compared to the 24-month-old animals (0.32 ± 0.16; 0.40 ± 0.15) (p < .05). The final testicular end velocity was lower in animals with long/moderate-shaped (L/M) (7.31 ± 2.91) than in those moderate/oval-shaped (M/O) (11.48 ± 3.88) testicles (p < .05). Animals with L/M testes presented higher pulsatility values and resistivity indices (0.51 ± 0.05; 0.55 ± 0.04) compared to animals with M/O shape (0.29 ± 0.20; 0.36 ± 0.15). We showed that the blood flow of the supra testicular artery between the two evaluated ages differed, and that 24-month-old bulls presented better thermoregulation capacity. Animals with a long/moderate testicular format presented a greater vascular resistance, which was imposed on the blood flow due to the anatomical differences in the testicular artery, resulting in lower velocity, and indicating better heat dissipation in this format.  相似文献   

Effect of water and nutrient supply on root distribution in an eucalyptus globulus plantation     

António Fabião  Manuel Madeira  Eliel Steen 《Water, air, and soil pollution》1990,54(1):635-640

Water and nutrients were supplied to an Eucalyptus globulus plantation in a controlled experiment in west central Portugal. The trees were planted in a sandy soil at a spacing of 3 × 3 m. The experiment consisted of four treatments: irrigation, irrigation plus fertilization, fertilization without irrigation, and a rain fed and unfertilized control. The quantification of root biomass was carried out 13 mo after planting.When the plantation was 31 mo old, a trench was dug in the soil to uncover the distribution of the roots of individual trees. In the irrigated treatments root biomass was higher than in the control and in the fertilized plots. Differences in fine and coarse root distribution along the soil profile were also observed. In both irrigated treatments roots were concentrated along tree rows, where the irrigation tubes were dripping water or water plus nutrients.  相似文献   

Partial purification and characterization of pectin methylesterase from acerola (Malpighia glabra L.)     

De Assis SA  Martins AB  Guaglianoni DG  De Faria Oliveira OM 《Journal of agricultural and food chemistry》2002,50(14):4103-4107

The enzyme pectin methylesterase (PME) is present in acerola fruit and was partially purified by gel filtration on Sephadex G-100. The results of gel filtration showed different PME isoforms. The total PME (precipitated by 70% salt saturation) and one of these isoforms (fraction from Sephadex G-100 elution) that showed a molecular mass of 15.5 +/- 1.0 kDa were studied. The optimum pH values of both forms were 9.0. The total and the partially purified PME showed that PME specific activity increases with temperature. The total acerola PME retained 13.5% of its specific activity after 90 min of incubation at 98 degrees C. The partially purified acerola (PME isoform) showed 125.5% of its specific activity after 90 min of incubation at 98 degrees C. The K(m) values of the total PME and the partially purified PME isoform were 0.081 and 0.12 mg/mL, respectively. The V(max) values of the total PME and the partially purified PME were 2.92 and 6.21 micromol/min/mL/mg of protein, respectively.  相似文献   

Glycaspis brimblecombei Moore, 1964 (Hemiptera: Psyllidae) on eucalyptus: oviposition non-preference and antibiosis     

Zulene Antônio Ribeiro  Bruno Henrique Sardinha de Souza  Eduardo Neves Costa  José Eduardo Petrilli Mendes  Reginaldo Gonçalves Mafia  Arlindo Leal Boiça Júnior 《Euphytica》2015,202(2):285-295

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Effect of stifle flexion on the position of the cranial tibial artery relative to the proximal tibia in dogs     

Ronan A. Mullins MVB  DECVS  Antía Escribano Carrera DVM  MSc  Jorge Espinel Rupérez LV  MS  PhD  Luca Bresciani DVM  PgC  Gareth I. Arthurs MA  VetMB  PGCertMedEd  DSAS  FHEA  Seamus Hoey MVB  DECVDI  DACVR 《Veterinary surgery : VS》2020,49(8):1527-1535

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