Combining ability for grain chemistry quality traits in a white oat diallelic cross     

Maraisa Crestani  Solange Ferreira da Silveira Silveira  Elisane Weber Tessmann  Itamara Mezzalira  Henrique de Souza Luche  José Antonio Gonzalez da Silva  Luiz Carlos Gutkoski  Fernando Irajá Félix de Carvalho  Antonio Costa de Oliveira 《Euphytica》2012,185(1):139-156

There has been a strong demand for oat genotypes that contain caryopsis with high chemical quality which can suit the different market niches. Therefore, the objectives of this study were to assess the general (GCA) and specific combining ability (SCA) of white oat cultivars through diallelic crosses providing information about the genetic effects on expression of grain chemical quality components. Also, it was aimed to estimate the heterosis on F₁ and F₂ generations and the vigor loss due to inbreeding. During 2008, 21 hybrid populations F₁ and F₂ were obtained from artificial crossing among seven Brazilian white oat cultivars, following the complete diallel design, without considering the reciprocals. These populations and their parents were evaluated in the 2009 season in the experimental field in Capão do Leão, RS, Brazil. The higher values of mean squares associated to GCA indicates a strong contribution of additive genetic effects to the expression of grain chemical components. The parents tested showed a tendency to develop progeny with negative heterosis regarding protein, lipid, β-glucan and soluble dietary fiber in the grain, and positive for the content of nitrogen-free extract, total and insoluble dietary fiber. IAC 7 features a potential parent for obtaining grains with high protein and dietary fiber content, and low caloric content, fit to human diet. Meanwhile, UPF 15 and FAPA Louise can represent donors of alleles to increase lipid contents, while FAPA Louise and URS Guapa can be used to raise the grain nitrogen-free extract contents of lines intended for animal feeding.  相似文献   

A gain-of-function polymorphism controlling complex traits and fitness in nature     

KV Prasad  BH Song  C Olson-Manning  JT Anderson  CR Lee  ME Schranz  AJ Windsor  MJ Clauss  AJ Manzaneda  I Naqvi  M Reichelt  J Gershenzon  SG Rupasinghe  MA Schuler  T Mitchell-Olds 《Science (New York, N.Y.)》2012,337(6098):1081-1084

Identification of the causal genes that control complex trait variation remains challenging, limiting our appreciation of the evolutionary processes that influence polymorphisms in nature. We cloned a quantitative trait locus that controls plant defensive chemistry, damage by insect herbivores, survival, and reproduction in the natural environments where this polymorphism evolved. These ecological effects are driven by duplications in the BCMA (branched-chain methionine allocation) loci controlling this variation and by two selectively favored amino acid changes in the glucosinolate-biosynthetic cytochrome P450 proteins that they encode. These changes cause a gain of novel enzyme function, modulated by allelic differences in catalytic rate and gene copy number. Ecological interactions in diverse environments likely contribute to the widespread polymorphism of this biochemical function.  相似文献   

Ribosome profiling shows that miR-430 reduces translation before causing mRNA decay in zebrafish   总被引：1，自引：0，他引：1  

Bazzini AA  Lee MT  Giraldez AJ 《Science (New York, N.Y.)》2012,336(6078):233-237

MicroRNAs regulate gene expression through deadenylation, repression, and messenger RNA (mRNA) decay. However, the contribution of each mechanism in non-steady-state situations remains unclear. We monitored the impact of miR-430 on ribosome occupancy of endogenous mRNAs in wild-type and dicer mutant zebrafish embryos and found that miR-430 reduces the number of ribosomes on target mRNAs before causing mRNA decay. Translational repression occurs before complete deadenylation, and disrupting deadenylation with use of an internal polyadenylate tail did not block target repression. Lastly, we observed that ribosome density along the length of the message remains constant, suggesting that translational repression occurs by reducing the rate of initiation rather than affecting elongation or causing ribosomal drop-off. These results show that miR-430 regulates translation initiation before inducing mRNA decay during zebrafish development.  相似文献   

Changing Concentrations of Heavy Metals in Stream Water as a Result of Slurry Application to Soil     

M.  Mercedes  Taboada-Castro  M.  Luz.  Rodriguez-Blanco Antonio  Dieguez M. Teresa  Taboada-Castro 《农业科学与技术》2012,(4):473-480

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Prevalence of and risk factors for bovine respiratory syncytial virus (BRSV) infection in non-vaccinated dairy and dual-purpose cattle herds in Ecuador     

Saa LR  Perea A  Jara DV  Arenas AJ  Garcia-Bocanegra I  Borge C  Carbonero A 《Tropical animal health and production》2012,44(7):1423-1427

A cross-sectional study was carried out to determine the seroprevalence and risk factors associated with bovine respiratory syncytial virus (BRSV) infection in non-vaccinated dairy and dual-purpose cattle herds from Ecuador. A total of 2,367 serum samples from 346 herds were collected from June 2008 to February 2009. A questionnaire, which included variables related to cattle, health, management measures, and the environment, was filled out in each herd. Presence of antibodies against BRSV was analyzed using a commercial indirect ELISA test. A logistic regression model was used to determine risk factors associated with BRSV at herd level. The individual seroprevalence against BRSV in non-vaccinated herds in Ecuador was 80.48% [1,905/2,367; 95% confidence interval (CI)?=?78.9-82.1]. The herd prevalence was 91.3% (316/346; 95% CI?=?88.3-94.3), and the intra-herd prevalence ranged between 25% and 100% (mean, 90.47%). The logistic regression model showed that the existence of bordering cattle farms, the dual-purpose farms, and the altitude of the farm (more than 2,338?m above sea level) were risk factors associated with BRSV infection. This is the first study about BRSV prevalence in Ecuador. It shows the wide spread of the BRSV infection in the country. The risk factors found will help to design effective control strategies.  相似文献   

A Biomechanical Comparison of Three Hybrid Linear‐Circular External Fixator Constructs     

Caleb C Hudson DVM  MS  Daniel D Lewis DVM  Diplomate ACVS  Alan R Cross DVM  Diplomate ACVS  Nicholas J Dunbar BSc  MaryBeth Horodyski EdD  ATC  LAT  Scott A Banks PhD  MS  Antonio Pozzi DMV  MS  Diplomate ACVS 《Veterinary surgery : VS》2012,41(8):954-965

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A common near infrared—based partial least squares regression model for the prediction of wood density of Pinus pinaster and Larix?×?eurolepis     

Ana Alves  Antonio Santos  Philippe Rozenberg  Luc E. Paques  Jean-Paul Charpentier  Manfred Schwanninger  José Rodrigues 《Wood Science and Technology》2012,46(1-3):157-175

Wood density is defined as the ratio of mass to volume and therefore in principle it should be possible to calculate a unique partial least squares regression (PLS-R) model for several species. PLS-R models for wood density based on X-ray microdensity data were calculated for each species Pinus pinaster and Larix × eurolepis and for both species together. After cross-validation and test set validation the data sets were combined and final models were calculated. The common model gave a residual prediction deviation (RPD) of 3.1, a range error ratio (RER) of 11.7, and a SEP/SEC of 1.06. The single models for Pinus pinaster and Larix?×?eurolepis gave RPD’s of 3.5 and 3.2, RER’s of 13 and 11, and a SEP/SEC of 1.2. To the best knowledge of the authors all obtained PLS-R models are the first ones that fulfil the requirements according to AACC Method 39-00 (AACC in AACC Method, 39-00:15, 1999) to be used at least for screening (RPD?≥?2.5). Although this method and the defined limits were developed for the analysis of grains they can be used as a rough rule of thumb until limits for wood are available. The improvement of the PLS-R models, compared to published results, might be due to three facts (1) the higher number of scans collected for a single spectrum, (2) that the samples were better represented by the NIR spectra and X-ray microdensity values, and (3) that the sites for the measurement of NIR spectra and X-ray microdensity were coincided as strictly as possibly.  相似文献   

Bilateral microphthalmia and aphakia associated with multiple eye abnormalities in a free-living European red deer calf (Cervus elaphus)     

Mutinelli F  Vercelli A  Carminato A  Luchesa L  Pasolli C  Cova M  Marchioro W  Melchiotti E  Vascellari M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(4):527-529

A free-living European red deer calf (Cervus elaphus) was euthanized due to bilateral microphthalmia. Lens was missing, replaced by proliferating squamous epithelial cells; hyperplastic squamous cells, sebaceous and mucinous glands were observed within the cornea with the characteristics of inclusion cyst. Findings were consistent with congenital microphthalmia/aphakia, with multiple eye abnormalities.  相似文献   

Potential for using Datura alba leaf extracts against two major stored grain pests, the khapra beetle Trogoderma granarium and the rice weevil Sitophillus oryzae     

Abid Ali  Farooq Ahmad  Antonio Biondi  Yusha Wang  Nicolas Desneux 《Journal of pest science》2012,85(3):359-366

Various plants are well known for their insecticidal activity and their use was maintained for millennia throughout all the agricultural regions of the world. In a current context, the use of Botanical insecticides represents one of the best alternatives to chemicals for the development of environmental-friendly strategies for stored grain pest control. Datura alba Nees is a plant found extensively in the warmer regions of the world and it is used as a medicinal plant. This study aims to assess the contact toxicity and the trans-generational effect of D.?alba leaf extract (DLE) against two important insect pests on stored rice, Trogoderma granarium and Sitophilus oryzae, under laboratory conditions. Filter papers were soaked in three DLE concentration solutions and in two control treatments: water and acetone. The survivor specimens (F₀) were transferred to a new untreated feeding substrate and the population build up of the two following generations (F₁ and F₂) were counted after 30 and 60?days, respectively. The highest DLE concentration (2.5?%) induced the significantly highest mortality with 33.5 and 45?% mortality in T.?granarium and S.?oryzae after 7?days of exposure, respectively. The DLE long-term effect toward both tested species was also proved by the high demographic decrease in the F₂ generations, when compared to control groups. This study is the first step toward establishing a scientific basis for the effective application of D.?alba plant materials as biorational tools to control stored grain pests.  相似文献   

Black yeast-like fungi associated with Lethargic Crab Disease (LCD) in the mangrove-land crab, Ucides cordatus (Ocypodidae)     

Vicente VA  Orélis-Ribeiro R  Najafzadeh MJ  Sun J  Guerra RS  Miesch S  Ostrensky A  Meis JF  Klaassen CH  de Hoog GS  Boeger WA 《Veterinary microbiology》2012,158(1-2):109-122

Lethargic Crab Disease (LCD) caused extensive epizootic mortality of the mangrove land crab Ucides cordatus (Brachyura: Ocypodidae) along the Brazilian coast, mainly in the Northeastern region. The disease was named after the symptoms of slow movement of infected crabs. Causative agents were suspected to be two black yeast-like fungi of the family Herpotrichiellaceae (ascomycete order Chaetothyriales), judged by infected tissue biopsies from moribund U. cordatus. The aim of the present study is to prove that two species are involved in the disease: the recently described black yeast Exophiala cancerae, but also a less virulent, hitherto undescribed fonsecaea-like species, introduced here as the novel species Fonsecaea brasiliensis. Strains were identified by ITS rDNA sequencing, and species borderlines were established by multilocus sequencing and AFLP analysis. Fonsecaea brasiliensis proved to be closely related to the pathogenic species Cladophialophora devriesii which originally was isolated from a systemic infection in a human patient. The virulence of F. brasiliensis is lower than that of E. cancerae, as established by artificial inoculation of mangrove crabs.  相似文献