A methodology for analyzing rare species distribution patterns utilizing GIS technology: The rare birds of Tanzania     

Ronald I. Miller  Simon N. Stuart  Kim M. Howell 《Landscape Ecology》1989,2(3):173-189

A simple, straightforward, cartographic modelling technique is presented for measuring relations between environmental characteristics and rare species distribution patterns. This approach is corroborated by digitizing rare bird distribution data for Tanzania and statistically analyzing these patterns in relation to geographic and environmental variables. Of the available natural resource data for Africa, only the vegetation and soils data appeared accurate enough to represent regional natural resource distribution patterns. Available data for Tanzania at the regional scale is not currently precise or comprehensive enough to analyze ongoing dynamic ecological processes.Statistical relations, associated with a study quadrangle within Tanzania, are documented for these parameters. Final confirmation of the accuracy of predictions about rare species diversity patterns will ensue from future field observations. When confirmed, this methodology can be used for setting conservation priorities in biologically little known regions of the world.  相似文献   

Experimental infections with fowl pox in chickens.     

L J Howell  R Hunter 《New Zealand veterinary journal》1978,26(11):270-272

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Effects of Larval Stocking Density on Laboratory-Scale and Commercial-Scale Production of Summer Flounder Paraliehthys dentatus     

Nicholas J.  King W. Huntting  Howell Marina  Huber David A.  Bengtson 《Journal of the World Aquaculture Society》2000,31(3):436-445

Abstract.— Three experiments investigating larval stocking densities of summer flounder from hatch to metamorphosis, Paralichthvs dentalus, were conducted at laboratory‐scale (75‐L aquaria) and at commercial scale (1,000‐L tanks). Experiments 1 and 2 at commercial scale tested the densities of 10 and 60 larvae/L, and 10, 20, and 30/L, respectively. The laboratory scale experiment tested the densities of 10, 20, 30, and 40 larvae/L. Experiments were carried out in two separate filtered, flow‐through seawater systems at URI Narragansett Bay Campus (laboratory‐scale), and at GreatBay Aquafarms, Inc. (commercial‐scale). At both locations, the larvae were raised in a “greenwater” culture environment, and fed rotifers and brine shrimp nauplii according to feeding regimes established for each location. Water temperature was maintained at 21C (± 2) and 19C (± 1) for the duration of laboratory and commercial experiments, respectively. Experiments 1 and 2 at the commercial location were terminated at 42 and 37 d post hatch (dph), respectively, and the laboratory experiment lasted 34 DPH. Larvae initially stocked at 10/L grew to an average length of 14.3 and 14.4 mm, and were significantly larger (P < 0.05) than those stocked at 30/L (13.1 mm) and 60/L (11.7 mm) in commercial scale experiments I and 2, respectively. At laboratory scale, no significant differences in length were detected, although mean total length tended to decrease with increasing stocking density (average length of 14.2, 13.3, 12.7, and 12.7 mm for treatments of 10, 20, 30, and 40/L, respectively). Final survival percentage was not affected by stocking density in either commercial experiment, and was 61 and 40% for treatments of 10 and 60/L in Experiment 1, respectively, and 62, 59, and 56% for Experiment 2, respectively. Similarly, there was no significant difference in final survival percentage among treatments in the laboratory experiment, which averaged 59, 55, 56, and 37% for treatments of 10, 20, 30, and 40L. respectively. Since larval length was not different between the intermediate densities (20 and 30 Iarvae/L), and because high‐density rearing can produce a much greater numerical yield per tank, we recommend a density of 30 larvaen as an optimal stocking density for the hatchery production of summer flounder.  相似文献   

Development of a snapback method of single-strand conformation polymorphism analysis for genotyping Golden Retrievers for the X-linked muscular dystrophy allele.     

K Honeyman  K S Carville  J M Howell  S Fletcher  S D Wilton 《American journal of veterinary research》1999,60(6):734-737

OBJECTIVE: To develop a snapback method of single-strand conformation polymorphism (SSCP) analysis for genotyping Golden Retrievers for the X-linked muscular dystrophy allele. ANIMALS: 20 Golden Retriever puppies from a colony with X-linked muscular dystrophy. PROCEDURE: DNA spanning the canine dystrophin mutation was amplified by means of a polymerase chain reaction (PCR), using a primer modified to have an additional sequence at the 5' terminus. The primer was designed so that 1 terminus of the single-stranded PCR product could anneal to the normal sequence flanking the region of the mutation in the allele but not in the mutant allele. True disease status of the dogs was determined by means of a PCR and restriction digest protocol. RESULTS: Snapback SSCP analysis allowed for accurate and unambiguous genotyping of unaffected, carrier, and affected dogs, whereas conventional SSCP analysis, using the unmodified primer, did not. Creatine kinase activities measured within 24 hours after birth were not consistent with genotype. CONCLUSION AND CLINICAL RELEVANCE: Snapback SSCP analysis provided a simple, fast, and accurate method for genotyping Golden Retrievers for the mutation known to cause X-linked muscular dystrophy.  相似文献   

The Effects of Temperature and Salinity on Early Life Stages of Black Sea Bass Centropristis striata     

David L.  Berlinsky Janalyn C.  Taylor  Rachel A.  Howell Terence M.  Bradley Theodore I. J.  Smith 《Journal of the World Aquaculture Society》2004,35(3):335-344

Along the Atlantic coast black sea bass occur from the Gulf of Maine to Florida and support important commercial and recreational fisheries. Interest in commercial production of black sea bass has increased in recent years due to high demand and limited seasonable availability. Efforts towards large-scale production have been hampered by a high incidence of early larval mortality. Two of the most important environmental variables affecting hatchery production of marine finfish larvae are temperature and salinity. In the wild, larval black sea bass are found in waters with temperatures of 12–24 C and salinity levels of 30–35 ppt. Studies were conducted to define the temperature and salinity ranges that support growth and development of black sea bass during early life stages. Three developmental phases were investigated: 1) fertilization to hatch: 2) hatch through yolk sac absorption: and 3) during the initial exogenous feeding stage (5–14 days post hatch: DPH). Fertilized eggs were obtained by manual spawning of fish following administration of LHRH_a. Fertilized eggs were transferred to 300-mL glass Petri dishes or 500-mL beakers to assess the effects of salinity and temperature through hatch and yolk sac absorption, respectively. To determine environmental effects on growth and survival during initial exogenous feeding 400 actively feeding larvae were cultured in green water and fed enriched rotifers for a 9-d period. For investigation of the effect of salinity, sea water (35 ppt) was diluted gradually to 15, 20, 25, and 30 ppt and maintained at 21 C. For examination of the effect of temperature, seawater was adjusted from 21 C to 12, 15, 21, 27, or 30 C at a rate of 3 C/h. No eggs hatched at 12 C or when salinity was maintained at 0 or 5 ppt. Hatching was uniformly high (≥ 85%) at temperatures between 15 and 27 C and at salinities ≥ 15 ppt. Survival through yolk sac absorption was greatest at temperatures between 18 and 27 C and at salinities ≥ 20 ppt. Survival through first feeding stage was highest at temperatures ≥ 18 C and 30 ppt salinity. Larval growth through first feeding was not significantly affected by salinity level but did increase with rearing temperature. The results indicate that survival and development of black sea bass during early life stages are most favorable at temperatures >18 C with salinity levels approaching full strength seawater.  相似文献   

Effect of antioxidants, citrate, and cryoprotectants on protein denaturation and texture of frozen cod (Gadus morhua)   总被引：4，自引：0，他引：4  

Badii F  Howell NK 《Journal of agricultural and food chemistry》2002,50(7):2053-2061

To investigate the role of antioxidants and cryoprotectants in minimizing protein denaturation in frozen lean fish, cod fillets were treated with either antioxidants (vitamin C (500 mg kg(-1)) or vitamin C (250 mg kg(-1)) + vitamin E (250 mg kg(-1))), antioxidants (vitamins C + E 250 mg kg(-1)each) with citrate (100 mg kg(-1)), cryoprotectants (4% (w/w) sucrose + 4% (w/w) sorbitol), or a mixture of antioxidants (vitamins C + E 250 mg kg(1)), citrate (100 mg kg(-1)), and cryoprotectants (sucrose 40 g kg(-1) + sorbitol 40 g kg(-1)). Untreated and treated fish samples were stored at -10 degrees C; cod fillets stored at -30 degrees C were used as a control. Stored frozen samples were analyzed at intervals for up to 210 days for changes in protein extractability, thermodynamic parameters (transition temperature T(m) and enthalpy DeltaH), structure by FT-Raman spectroscopy, and rheological properties by large and small deformation tests. Results indicated that protein denaturation and texture changes were minimized in the presence of cryoprotectants, as well as in the presence of antioxidants with citrate, antioxidants alone, or the mixture of antioxidants, citrate, and cryoprotectants. In the presence of increased formaldehyde levels in fish treated with vitamin C, toughening was still lower compared to that of the -10 degrees C control due to the antioxidant property of vitamin C. Thus, ice crystal formation and lipid oxidation products are the major factors that cause protein denaturation in lean frozen fish, and antioxidants in addition to cryoprotectants can be used to minimize toughness.  相似文献   

Patterns of water chemistry among twenty-seven oligotrophic lakes in Kejimkujik National Park,Nova Scotia     

B. Freedman  J. Kerekes  G. Howell 《Water, air, and soil pollution》1989,46(1-4):119-130

A cluster analysis was used to apportion 27 oligotrophic lakes in southwestern Nova Scotia into five multivariate groups on the basis of patterns of covariation of 11 chemical variables (Cl, SO₄, Gran alkalinity, organic anions, Ca, Mg, K, Na, H⁺, and color). These groups are described in terms of the average values of the chemical variables. The relationships among the groups were investigated by an ordination by detrended correspondence analysis. The first, and by far the strongest axis of the ordination separated lakes with relatively large concentrations of alkalinity, from strongly colored lakes with large concentrations of H⁺ and organic anions. Axis 2 separated acidic lakes, from lakes with large concentrations of Ca and alkalinity.  相似文献   

Response of sugarbeets to irrigation frequency and cutoff on a clay loam soil     

T. A. Howell  L. H. Ziska  R. L. McCormick  L. M. Burtch  B. B. Fischer 《Irrigation Science》1987,8(1):1-11

Summary Sugarbeets (Beta vulgaris L.) on a Panoche clay loam soil were subjected to 3 different irrigation frequencies and 3 irrigation cutoff dates prior to harvest to determine the effects on evapotranspiration, growth, and sucrose yield. Lengthening the irrigation interval from 1 to 3 weeks reduced evapotranspiration without a significant decline in sucrose production. Increased irrigation cutoff from 3 to 7 weeks prior to harvest significantly increased sucrose percentage within the root and resulted in similar total sucrose yields. Lengthening the irrigation interval only slightly reduced both fresh vegetative biomass and leaf area index (significant differences occurred only at one plant sampling date). The combination of less frequent irrigation and an early cutoff date increased the amount of soil water extracted by sugarbeets. The water use of sugarbeets can be reduced without a significant decline in sucrose production through optimizing irrigation frequency to about 14 to 20 days on this soil and cutting off irrigations about 40 to 45 days before harvest, provided irrigations replenish soil water depletions.Contribution from USDA, Agricultural Research Service, Water Management Res. Laboratory, 2021 S. Peach Avenue, Fresno, CA 93747, USA  相似文献   

Mesothelioma in horses     

C M Colbourne  J R Bolton  J N Mills  D Whitaker  J V Yovich  J M Howell 《Australian veterinary journal》1992,69(11):275-278

Pleural mesothelioma and pericardial mesothelioma with spread to the pleural cavity were diagnosed in 2 horses. Clinical signs included respiratory distress, depression and pleural pain. The affected body cavities contained copious, serous effusions containing very large neoplastic mesothelial cells with densely-staining cytoplasm, hyperchromic nuclei and prominent, often multiple, nucleoli. Ultrastructurally, the neoplastic cells appeared as solid aggregates, with profuse microvillous borders and neolumen formation. Cytological examination, together with confirmative ancillary diagnostic tests, including electron microscopy and immunocytochemistry, may be used to obtain an ante-mortem diagnosis of mesothelioma.  相似文献   

THE CHEMICAL REGULATION OF THE PROCESSES OF THE BODY BY MEANS OF ACTIVATORS,KINASES AND HORMONES     

Howell WH 《Science (New York, N.Y.)》1910,31(786):93-100

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