A genome‐wide association study for fat‐related traits computed by image analysis in Japanese Black cattle          下载免费PDF全文

Ayaka Nakajima  Fuki Kawaguchi  Yoshinobu Uemoto  Moriyuki Fukushima  Emi Yoshida  Eiji Iwamoto  Takayuki Akiyama  Namiko Kohama  Eiji Kobayashi  Takeshi Honda  Kenji Oyama  Hideyuki Mannen  Shinji Sasazaki 《Animal Science Journal》2018,89(5):743-751

The objective of this study was to identify genomic regions associated with fat‐related traits using a Japanese Black cattle population in Hyogo. From 1836 animals, those with high or low values were selected on the basis of corrected phenotype and then pooled into high and low groups (n = 100 each), respectively. DNA pool‐based genome‐wide association study (GWAS) was performed using Illumina BovineSNP50 BeadChip v2 with three replicate assays for each pooled sample. GWAS detected that two single nucleotide polymorphisms (SNPs) on BTA7 (ARS‐BFGL‐NGS‐35463 and Hapmap23838‐BTA‐163815) and one SNP on BTA12 (ARS‐BFGL‐NGS‐2915) significantly affected fat percentage (FAR). The significance of ARS‐BFGL‐NGS‐35463 on BTA7 was confirmed by individual genotyping in all pooled samples. Moreover, association analysis between SNP and FAR in 803 Japanese Black cattle revealed a significant effect of SNP on FAR. Thus, further investigation of these regions is required to identify FAR‐associated genes and mutations, which can lead to the development of DNA markers for marker‐assisted selection for the genetic improvement of beef quality.  相似文献   

Foot rot disease of sweet potato in Japan caused by Diaporthe destruens: first report,pathogenicity and taxonomy     

Maeda  Ai  Minoshima  Ayaka  Kawano  Shinji  Nakamura  Misa  Takushi  Tetsuya  Yamashiro  Maki  Kawamura  Futoshi  Oshiro  Atsushi  Ichinose  Katsuya  Okada  Yoshihiro  Rossman  Amy Y.  Hirooka  Yuuri 《Journal of General Plant Pathology》2022,88(1):33-40

Journal of General Plant Pathology - Foot rot, also known as stem rot or tuber-root rot syndrome, on sweet potato occurs in sweet potato fields in Okinawa, Japan. Isolates of one fungal species...  相似文献   

Successful outcome after a single endoscopic fecal microbiota transplantation in a Shiba dog with non-responsive enteropathy during the treatment with chlorambucil     

Koji SUGITA  Ayaka SHIMA  Kaho TAKAHASHI  Yasuyoshi MATSUDA  Masaki MIYAJIMA  Marin HIROKAWA  Hirotaka KONDO  Junpei KIMURA  Genki ISHIHARA  Keitaro OHMORI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(6):984

A 7-year 6-month-old, castrated male Shiba dog presented with a 1-month history of lethargy, anorexia, vomiting, and frequent watery diarrhea. Weight loss, hypoalbuminemia, anemia, and leukocytosis were detected at the first visit. The dog was diagnosed with non-responsive enteropathy (NRE) based on clinical and histopathological examinations. Since the dog did not respond to the immunosuppressive drugs, fecal microbiota transplantation (FMT) was performed during the treatment with chlorambucil. A single endoscopic FMT into the cecum and colon drastically recovered clinical signs and clinicopathological abnormalities and corrected dysbiosis in the dog. No recurrence or adverse events were observed. The present case report suggests that FMT, possibly together with chlorambucil, might be a treatment option for NRE in Shiba dogs that have poorer prognosis compared with other dog breeds.  相似文献