Schirmer tear test I and rebound tonometry findings in healthy calves          下载免费PDF全文

Kyle L. Tofflemire  Elizabeth M. Whitley  Stacie A. Gould  Renee D. Dewell  Rachel A. Allbaugh  Gil Ben‐Shlomo  Annette M. O'Connor  R. David Whitley 《Veterinary ophthalmology》2015,18(2):147-151

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Anhydrous goat's milk fat: thermal and structural behaviors studied by coupled differential scanning calorimetry and X-ray diffraction. 2. Influence of cooling rate     

Amara-Dali WB  Lesieur P  Artzner F  Karray N  Attia H  Ollivon M 《Journal of agricultural and food chemistry》2007,55(12):4741-4751

Crystallization and melting properties of triacylglycerols (TGs) in anhydrous goat's milk fat (AGMF) are investigated by X-ray diffraction as a function of temperature (XRDT) coupled with high-sensitivity differential scanning calorimetry (DSC), using synchrotron radiation and Microcalix. The polymorphic behavior of AGMF was monitored by varying the cooling rates between 5 and 1 degrees C/min from 45 to -20 degrees C with their subsequent melting at 1 degrees C/min. Quenching of AGMF at -20 degrees C was also examined to determine the metastable polymorphic form of AGMF. At intermediate cooling rates, TGs in AGMF crystallize, from about 18 degrees C in two different lamellar structures with triple chain length 3Lalpha stacking of 72 A and a double chain length 2Lalpha stacking of 48 A, which are correlated to two overlapped exothermic peaks recorded by DSC. A reversible transition sub alpha <--> alpha was observed. Subsequent heating at 1 degrees C/min shows numerous structural rearrangements before final melting. At fast cooling of AGMF (5 degrees C/min), similar unstable crystalline varieties are formed while three endotherms are recorded. Several new unstable lamellar structures are observed after quenching. All of these data are compared to those previously reported at slow cooling (0.1 degrees C/min) showing a relative stability of the structures formed. In spite of general similitude, the thermal and structural behavior of the goat's milk is more complex than that of the cow's milk.  相似文献   

沿淮稻茬麦区小麦的产量及籽粒灌浆特性     

姜沣益  王犇  任开明  李哲  黄正来  张文静  马尚宇  樊永惠 《安徽农业大学学报》2022,49(3):395

为给沿淮稻茬麦区小麦高产栽培及品种育种提供依据,在大田条件下,选取沿淮麦区6个高产小麦品种为供试材料,用三次多项式方程对籽粒灌浆过程进行拟合,通过相关性分析对小麦籽粒灌浆参数与粒重的关系进行探究,研究不同小麦品种间产量及其构成因素的差异。结果表明： 6个品种中,安农大1216的产量最高,为8 598.77 kg·hm^-²,烟农19最低,产量为6 031.07 kg·hm^-²;籽粒灌浆过程中安农大1216粒重最高,成熟期千粒重达到51.4 g,郑麦9023的千粒重最低,为47.78 g;各个品种间灌浆速率存在明显差异。不同小麦品种籽粒灌浆过程均呈“S”型变化,因品种不同,灌浆参数也有所不同,但“R²”均达到0.99以上。相关性分析表明,千粒重与理论最大粒重（W）显著相关,与平均灌浆速率（V）、最大灌浆速率（V_max）、有效灌浆持续期平均灌浆速率（Vs）、有效灌浆持续期粒重增加值（Ws）和最大灌浆速率出现时间（T）呈正相关但未达显著水平。可见筛选的W、V、V_max、Vs、Ws和T等灌浆参数值较高的小麦品种在该地区种植有利于提高小麦千粒重,增加产量。此外,必须考虑到小麦品种、高效栽培方式、管理手段和协调产量构成三因素,才会最大可能实现小麦高产高效。  相似文献   

评估鱼种质量的方法   总被引：1，自引：0，他引：1       下载免费PDF全文

张本 《水产学报》1980,4(4):329-338

本文分析研究了鲢、鳙、草鱼、鳊鱼鱼种的体重与体长的相关关系，以及它们与鱼体肥满度、体高率和体厚率的相关关系，得出：鱼种体重的大小能比较正确地反映出鱼种质量的优劣，而体长仅是鱼种质量的参比指标。利用鱼种体重与体长相关点分布面的上周界线、中值线和下周界线的数学方程式，可以推算出各种规格鱼种的优、中、劣的标准，即以鱼种的相对体重来评估鱼种的质量。  相似文献   

海水经济鱼类遗传育种研究进展   总被引：1，自引：0，他引：1  

尹绍武  黄海  雷从改  陈国华  张本 《水产科学》2007,26(7):416-419

近二三十年来，海水养殖方式与设备的不断发展与完善，促进了海水养殖业的发展。但是，由于海水经济鱼类缺乏优良品种，大多数养殖种类为野生种或半野生种，许多养殖种群在累代繁殖后，出现品质退化、生长速度慢、发病率高等不良症状，严重地阻碍着海水经济鱼类养殖业的发展。因此，近年来，世界各国纷纷加强海水鱼类的遗传育种研究工作，  相似文献   

Genotypic characterization by spoligotyping and VNTR typing of Mycobacterium bovis and Mycobacterium caprae isolates from cattle of Tunisia     

Hela Lamine-Khemiri  Remigio Martínez  Waldo Luis García-Jiménez  Jose Manuel Benítez-Medina  Maria Cortés  Inés Hurtado  Mohammed Salah Abassi  Imed Khazri  Mohammed Benzarti  Javier Hermoso-de-Mendoza 《Tropical animal health and production》2014,46(2):305-311

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In silico prediction of Gallibacterium anatis pan-immunogens     

Ragnhild J Bager  Egle Kudirkiene  Isabelle da Piedade  Torsten Seemann  Tine K Nielsen  Susanne E Pors  Andreas H Mattsson  John D Boyce  Ben Adler  Anders M Bojesen 《Veterinary research》2014,45(1)

The Gram-negative bacterium Gallibacterium anatis is a major cause of salpingitis and peritonitis in commercial egg-layers, leading to reduced egg production and increased mortality. Unfortunately, widespread multidrug resistance and antigenic diversity makes it difficult to control infections and novel prevention strategies are urgently needed. In this study, a pan-genomic reverse vaccinology (RV) approach was used to identify potential vaccine candidates. Firstly, the genomes of 10 selected Gallibacterium strains were analyzed and proteins selected on the following criteria; predicted surface-exposure or secretion, none or one transmembrane helix (TMH), and presence in six or more of the 10 genomes. In total, 42 proteins were selected. The genes encoding 27 of these proteins were successfully cloned in Escherichia coli and the proteins expressed and purified. To reduce the number of vaccine candidates for in vivo testing, each of the purified recombinant proteins was screened by ELISA for their ability to elicit a significant serological response with serum from chickens that had been infected with G. anatis. Additionally, an in silico prediction of the protective potential was carried out based on a protein property prediction method. Of the 27 proteins, two novel putative immunogens were identified; Gab_1309 and Gab_2312. Moreover, three previously characterized virulence factors; GtxA, FlfA and Gab_2156, were identified. Thus, by combining the pan-genomic RV approach with subsequent in vitro and in silico screening, we have narrowed down the pan-proteome of G. anatis to five vaccine candidates. Importantly, preliminary immunization trials indicated an in vivo protective potential of GtxA-N, FlfA and Gab_1309.

Electronic supplementary material

The online version of this article (doi:10.1186/s13567-014-0080-0) contains supplementary material, which is available to authorized users.  相似文献   

Early weaning of winter flounder (Pseudopleuronectes americanus Walbaum) larvae on a commercial microencapsulated diet   总被引：1，自引：0，他引：1  

I Ben Khemis  C Audet  R Fournier  & J de la Noüe 《Aquaculture Research》2003,34(6):445-452

Like most small marine fish larvae, the stomachs of winter flounder Pseudopleuronectes americanus are undeveloped at first feeding and have relatively reduced digestive capacity. This work was undertaken to test whether larvae at the onset of stomach differentiation (larval size about 5.5 mm) could be early weaned onto a commercial microencapsulated diet. We assessed the effect of early weaning by first comparing growth performance (standard length, total protein content and age at metamorphosis) of larvae fed enriched live prey from first feeding to a size of 5.5 mm and then reared on three different feeding regimes until metamorphosis: (1) live prey (LP) as a control group; (2) mixed feeding of live prey and microencapsulated diet (LP‐ME); (3) exclusively microencapsulated diet (ME) after fast weaning over 4 days (to a larval size of 6.2 mm). No differences were observed between larval development in the two first groups, which began metamorphosis at 40 days old. The larvae of the third group showed significantly slower growth that resulted in a delay of 4 days in the onset of metamorphosis. Differences in live prey availability between the treatments and the short transition period to allow the larvae to adapt to the new diet were identified as possible contributing factors to the slower growth and to the delay in metamorphosis of early weaned larvae. In a second experiment, the transitional weaning period was increased until the larvae were 6.6 mm in length. Weaning at that size resulted in no slowing of growth or delay in metamorphosis, suggesting that the feeding schedule was adequate.  相似文献   

The diving behaviour of little penguins in Western Australia predisposes them to risk of injury by watercraft     

Belinda Cannell  Yan Ropert‐Coudert  Ben Radford  Akiko Kato 《水产资源保护:海洋与淡水生态系统》2020,30(3):461-474

1. The most western little penguin colony globally, and the most northern in Western Australia (WA) is found on Penguin Island, WA. The penguins use coastal bays that are also used extensively by recreational watercraft. These penguins have been found to either dive predominantly to shallow depths of 1–5 m or to depths >8 m. It is thus hypothesized that (a) both the shallow and deeper diving penguins can potentially be disturbed or injured by these watercraft but that the risk will differ between the two diving strategies, and (b) that risk of injury for both is greater during the summer and autumn, when people are more likely to use watercraft.
2. This was tested by attaching data loggers to little penguins during chick rearing and by investigating necropsy records. Diving activity was studied for the very shallow and relatively deeper diving penguins separately, and we considered the penguins were vulnerable to interactions with watercraft when they were within the top 2 m of the water column or at the surface.
3. Shallow‐diving penguins executed >1,200 dives per day, 64% of dives occurred within the top 2 m, and they were vulnerable for approximately two‐thirds of their time at sea. The deeper diving penguins executed fewer dives. Almost half of dives were to ≥10 m, yet they were vulnerable for almost one‐third of their time at sea. Their post‐dive recovery was also longer. Thus, the risk of interaction from watercraft differs depending on the diving behaviour.
4. This study highlights the potential impact to little penguins throughout Australia and New Zealand.

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Comparison of traditional and environmental DNA survey methods for detecting rare and abundant freshwater fish     

Maxine P. Piggott  Samuel C. Banks  Ben T. Broadhurst  Christopher J. Fulton  Mark Lintermans 《水产资源保护:海洋与淡水生态系统》2021,31(1):173-184

1. Detecting rare species is often a necessity for conservation and management, yet challenging for many field survey methods. Environmental DNA (eDNA) is a highly promising solution that has been shown to outperform many established survey methods.
2. Macquarie perch (Macquaria australasica) is an endangered native species that has declined significantly in range and abundance. Detection of M. australasica was compared with an abundant alien fish species (Oncorhynchus mykiss) using eDNA and three conventional survey methods: gill nets, electrofishing and fyke nets.
3. eDNA occupancy estimates for both fish species were compared using four different models to investigate what effect these differences have on false positives and false negatives for the rare and common fish species. These models used unadjusted eDNA detections in water samples, eDNA detections that have been screened using a limit of detection method to remove potential false positives, eDNA data supplemented with a second survey method, or eDNA data augmented with sequencing of positive polymerase chain reaction replicates.
4. eDNA surveying as a single detection method was found to be more efficient and sensitive compared with each capture method separately and combined. Occupancy estimates for the common and rare species did not vary significantly between the four site occupancy-detection models, suggesting that supplementary data may not have as much effect on occupancy estimates compared with other approaches such as temporal or spatial sampling.
5. We conclude that eDNA outperforms the three established survey methods for both a rare and common freshwater fish species. Although there was no significant effect of augmenting eDNA survey methods with other survey data, additional data may improve confidence in detection, and provide confirmatory evidence for unexpected or new detections of a species.

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