p-Dimethylaminobenzenediazo sodium sulfonate (Fenaminosulf)-reductase from Pseudomonas fragi Bk₉     

N.G.K. Karanth  S.G. Bhat  C.S. Vaidyanathan  V.N. Vasantharajan 《Pesticide biochemistry and physiology》1976,6(1):20-29

p-Dimethylaminobenzenediazo sodium sulfonate (DABDS)-reductase catalyzing the conversion of DABDS to N,N-dimethyl-p-phenylenediamine was purified to homogeneity from the cell-free extracts of Pseudomonas fragi Bk₉, crystallized, and its properties studied. The homogeneity of the enzyme was ascertained by gel electrophoresis and immunodiffusion studies. The molecular weight of the enzyme as determined by the gel filtration method was found to be approximately 87,000. The enzyme was optimally active at pH 7 and 45°C, with an activation energy of 2.5 kcal/mol. NADH, NADPH, and GSH could not function as cofactors, while the enzyme required dithiothreitol as an electron donor. From the Lineweaver-Burk plots, the K_m values were calculated to be 0.90 and 3.53 mM for DABDS and dithiothreitol, respectively. The enzyme did not show any requirement for metal ions and was inhibited to varying degrees by different sulfhydryl reagents.  相似文献   

Response of vanilla (Vanilla planifolia A.) intercropped in arecanut to irrigation and nutrition in humid tropics of India   总被引：1，自引：0，他引：1  

S. Sujatha  Ravi Bhat 《Agricultural Water Management》2010,97(7):988-994

A 5-year field trial to assess the impact of microsprinkler irrigation and nutrition on vanilla grown as intercrop in arecanut plantation was conducted on a laterite soil. Pooled analysis indicated that microsprinkler irrigation at 1.0 Epan resulted in significantly higher green bean yield (842 kg ha⁻¹) than 0.75 Epan (579 kg ha⁻¹). Organic manure application in the form of vermicompost (720 kg ha⁻¹) and FYM (768 kg ha⁻¹) and recommended NPK (718 kg ha⁻¹) produced green bean yield at par with recycling of gliricidia prunings (625 kg ha⁻¹). Irrigation at 1.0 Epan proved superior by registering maximum benefit:cost (B:C) ratio of 2.25 compared to 1.62 at 0.75 Epan. The highest B:C ratio was obtained with recommended NPK (2.27) followed by recycling of gliricidia prunings (2.10), vermicompost (1.87), vermicompost + arecanut husk mulching (1.80) and FYM (1.64). The soil pH increased by 0.4 units in 2008 compared with the pre-experimental soil pH of 5.6 in 2004. Nutrition alone and in combination with irrigation had significant impact on soil pH. Organic manure application increased the soil pH (6.1-6.2) significantly over recommended NPK (5.6) at the end of experiment in 2008. Significant variation in soil organic carbon (SOC) was noticed due to different nutrition treatments. Application of vermicompost and FYM significantly increased the SOC content by 38-54% in 2008 over initial levels in 2004. Bray's P availability was influenced by nutrition and its interaction with irrigation. Application of FYM continuously for 4 years has resulted in significant increase in Bray's P content (41.3 mg kg⁻¹) compared to other nutrition treatments (9.4-17.2 mg kg⁻¹). Irrigation equivalent to 0.75 Epan (223 mg kg⁻¹) increased the K availability significantly over 1.0 Epan (172 mg kg⁻¹). The K availability was significantly higher in recommended NPK (416 mg kg⁻¹) than in other organic treatments (98-223 mg kg⁻¹) at 0-30 cm soil depth. Overall, vanilla responded well to irrigation and nutrition in arecanut-based cropping system with a better economic output and improved soil fertility.  相似文献   

Incidence of cysts in pars distalis of mongrel dogs     

R R Rao  N G Bhat 《The Indian veterinary journal》1971,48(2):128-133

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Pre- and postinvasion defenses both contribute to nonhost resistance in Arabidopsis     

Lipka V  Dittgen J  Bednarek P  Bhat R  Wiermer M  Stein M  Landtag J  Brandt W  Rosahl S  Scheel D  Llorente F  Molina A  Parker J  Somerville S  Schulze-Lefert P 《Science (New York, N.Y.)》2005,310(5751):1180-1183

Nonhost resistance describes the immunity of an entire plant species against nonadapted pathogen species. We report that Arabidopsis PEN2 restricts pathogen entry of two ascomycete powdery mildew fungi that in nature colonize grass and pea species. The PEN2 glycosyl hydrolase localizes to peroxisomes and acts as a component of an inducible preinvasion resistance mechanism. Postinvasion fungal growth is blocked by a separate resistance layer requiring the EDS1-PAD4-SAG101 signaling complex, which is known to function in basal and resistance (R) gene-triggered immunity. Concurrent impairment of pre- and postinvasion resistance renders Arabidopsis a host for both nonadapted fungi.  相似文献   

Inhibition of entry of HIV-1 in neural cell lines by antibodies against galactosyl ceramide.   总被引：54，自引：0，他引：54  

J M Harouse  S Bhat  S L Spitalnik  M Laughlin  K Stefano  D H Silberberg  F Gonzalez-Scarano 《Science (New York, N.Y.)》1991,253(5017):320-323

Although the CD4 molecule is the principal cellular receptor for the human immunodeficiency virus (HIV), several CD4-negative cell lines are susceptible to infection with one or more HIV strains. These findings indicate that there are alternate modes of viral entry, perhaps involving one or more receptor molecules. Antibodies against galactosyl ceramide (galactocerebroside, or GalC) inhibited viral internalization and infection in two CD4-negative cell lines derived from the nervous system: U373-MG and SK-N-MC. Furthermore, recombinant HIV surface glycoprotein gp120 bound to GalC but not to other glycolipids. These results suggest a role for GalC or a highly related molecule in HIV entry into neural cells.  相似文献   

AFLP Analysis of Genetic Diversity in Indian Soybean [Glycine max (L.) Merr.] Varieties     

C. Tara Satyavathi  K. V. Bhat  C. Bharadwaj  S. P. Tiwari  V. K. Chaudhury 《Genetic Resources and Crop Evolution》2006,53(5):1069-1079

AFLP technique was used to assess genetic diversity in 72 soybean varieties under cultivation in India. Selected 12 AFLP primer pairs produced 1319 products of which 1257 were polymorphic (95%). Wide variations were observed for the number of amplification products, percent polymorphism and average polymorphism information content (PIC). The Jaccard's similarity indices (J) based on the AFLP profiles of the 72 soybean varieties were subjected to UPGMA cluster analysis. The dendrogram generated revealed four major clusters, which were strongly supported by the high bootstrap values obtained from analyses of 1000 bootstrap samples. In addition, the Mantel's test for cophenetic correlation with r = 0.955 indicated very good fit of the varieties to a group in the cluster analysis. Some correspondence between the clustering pattern and the pedigree, place of release or target area of the variety was observed. Overall moderately high genetic diversity was observed which appears to be due to the higher genetic diversity prevalent in 12 of the varieties included in three diverse clusters and was indicative of the need to include more diverse germplasm in the soybean improvement programs.  相似文献   

Studies on Management of Chromium(VI) – Contaminated Industrial Waste Effluent using Hydrous Titanium Oxide (HTO)     

Ghosh  Uday Chand  Dasgupta  Manotosh  Debnath  Sushanta  Bhat  Subhas Chandra 《Water, air, and soil pollution》2003,143(1-4):245-256

The anion exchange behaviour of hydrous titanium oxide(HTO) has been exploited for the management of industrialwaste effluents contaminated with chromium(VI). Theadsorption of chromium(VI) by HTO (74.0–140.0 micron) in thepH range 0.5–8.0 has been studied. It is found that theadsorption of chromium(VI) by HTO is at a maximum in the pHrange 1.5–2.0. The interference of diverse foreign ionssuch as nitrate, chloride, sulfate, phosphate, calcium,magnesium, nickel, iron(III), barium etc. on the adsorptionof chromium(VI) by HTO at optimum pH has been investigatedby a batch-operation technique. Break-through capacity,adsorption and elution of chromium(VI) using HTO have beenstudied. It is found that HTO could be reused as anadsorbent for chromium(VI). Finally, chromium(VI) wasrecovered as insoluble chromate compound from waste effluentof Hindustan Motor Limited (HML) of Hooghly, West Bengal, India.  相似文献   

Potential therapeutic applications of some antinutritional plant secondary metabolites   总被引：8，自引：0，他引：8  

Singh B  Bhat TK  Singh B 《Journal of agricultural and food chemistry》2003,51(19):5579-5597

Plant-based formulations have been used since ancient times as remedial measures against various human and animal ailments. Over the past 20 years interest in traditional medicines has increased considerably in many parts of the world. Whereas modifications in lifestyles, including diet, have had a profound effect on the increased risks of various diseases, there is considerable scientific evidence, both epidemiological and experimental, regarding vegetables and fruits as key features of diets associated with reduced risks of diseases such as cancers and infections. This has led to the use of a number of phytometabolites as anticarcinogenic and cardioprotective agents, promoting a dramatic increase in their consumption as dietary supplements. There are changing perceptions regarding the therapeutic potential of various plant secondary metabolites (PSMs), some of which have also been known to possess certain antinutritional qualities. The knowledge gained at the cellular and molecular levels, and biological activities of PSMs including tannin-polyphenols, saponins, mimosine, flavonoids, terpenoids, and phytates, would be useful in planning for future epidemiological studies and human cancer prevention trials, especially when a large pure dosage is not the option to deliver the active compounds to many tissues. It is well observed that alteration of cell cycle regulatory gene expression is frequently found in tumor tissues or cancer cell lines, and studies have suggested that the herbal-based or plant-originated cell cycle regulators might represent a new set of potential targets for anticancer drugs. The recent upsurge of interest in this area of research and advances made therein indicate that the impact of a number of diseases affecting humans and animals may be lessened, if not prevented, by simple dietary intake of PSMs with putative therapeutic properties.  相似文献   

Organization and expression of the mitochondrial genome in CMS (Moricandia) Brassica juncea: nuclear-mitochondrial incompatibility results in differential expression of the mitochondrial atpα gene     

K. Gaikwad    A. Baldev    P. B. Kirti    T. Mohapatra    S. R. Bhat    V. Chopra    S. Prakash 《Plant Breeding》2006,125(6):623-628

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Validation of markers linked to late leaf spot and rust resistance,and selection of superior genotypes among diverse recombinant inbred lines and backcross lines in peanut (Arachis hypogaea L.)     

M. Sukruth  S. A. Paratwagh  V. Sujay  V. Kumari  M. V. C. Gowda  H. L. Nadaf  B. N. Motagi  S. Lingaraju  M. K. Pandey  R. K. Varshney  R. S. Bhat 《Euphytica》2015,204(2):343-351

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