Efficacy of a transmissible gastroenteritis coronavirus with an altered ORF-3 gene.   总被引：1，自引：0，他引：1  

R D Woods 《Canadian journal of veterinary research》2001,65(1):28-32

Serial passage of virulent transmissible gastroenteritis virus through cell culture reduced its virulence in 3-day-old piglets. Intramuscular inoculation of pregnant gilts with 2 doses of this modified-live virus elicited a level of lactogenic immunity that protected their nursing piglets against a lethal dose of challenge virus. Sequence analysis of a 637-bp fragment of the spike gene containing most of the aminopeptidase receptor and the 4 major antigenic sites from the original and the serially passed viruses were nearly identical. Gel analysis revealed that the fragment from the ORF-3 gene of virulent virus was smaller than the corresponding fragment from the serially passed virus. Sequence analysis of the fragment from the passed virus revealed that the sequence between nt 5310 and nt 5434 was replaced by a 636-bp fragment from the polymerase 1A gene. This replacement resulted in the loss of the CTAAACTT leader RNA-binding site and ATG start codon for the ORF-3A gene but it did not affect the ORF-3B gene.  相似文献   

Neospora-like protozoal infections associated with abortion in goats.   总被引：7，自引：0，他引：7  

B C Barr  M L Anderson  L W Woods  J P Dubey  P A Conrad 《Journal of veterinary diagnostic investigation》1992,4(3):365-367

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Renewal and regeneration     

Bill Scanlan 《Australian veterinary journal》1997,75(4):243-243

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A procedure for combining a quantitative inheritance study with the first cycle of a breeding program     

M. J. Bassett  F. E. Woods 《Euphytica》1978,27(1):295-303

Summary The number of effective factors controlling the inheritance of a quantitative character can be estimated by analysis of F₃ lines where the parental lines are homozygous. The procedure is illustrated with data from a study of pod length inheritance in common bean (Phaseolus vulgaris L.). The mean pod length of Sprite, the parent with longer pods, was compared statistically with the means of F₃ progenies derived from F₂ plants obtained from the appropriate tail of the F₂ frequency distribution for pod length. The data indicate that analysis of F₃ progenies avoids much of the underestimation of the effective factor number which is characteristic of other procedures. The number of effective factors was estimated to be a minimum of 4 and the correlation between random F₂ parents and F₃ progeny means was r=0.82.Florida Agricultural Experiment Station Journal Series No. 406.  相似文献   

The use of seed acid phosphatases in the determination of the purity of F1 hybrid brussels sprout seed     

S. Woods  D. A. Thurman 《Euphytica》1976,25(1):707-712

Summary Starch gel electrophoresis of single seeds of five different F₁ hybrids of Brussels sprouts showed that they possessed three cathodal acid phosphatases. By comparison with cathodal acid phosphatases present in their inbred parents these have been interpreted as the two parental types plus a hybrid enzyme. All of the parental material could be classified into two groups depending upon whether or not their cathodal acid phosphatase was fast or slow moving. It was shown that these acid phosphatases are suitable for the determination of sibs in F₁ hybrid sprout seed provided that one of the parents possesses the slow moving cathodal acid phosphatase and the other the fast moving one. A survey of 35 different F₁ hybrids showed that 18 could be analysed for sibs using this method, those which could not were assumed to have had parents who possessed cathodal acid phosphatases of the same mobilities.  相似文献   

A spatial analysis of forest management and its contribution to maintaining the extent of shrubland habitat in southern New England, United States     

Bill Buffum  Scott R. McWilliamsPeter V. August 《Forest Ecology and Management》2011,262(9):1775-1785

Conservation organizations in the northeastern United States (US) recommend forest clearcutting to create shrubland habitat, which is required by many wildlife species with declining populations. The planning of habitat management programs is hampered by a lack of information on the current extent of shrubland habitat and the current rate of forest clearcutting that creates shrubland habitat. We addressed these information gaps by using a combination of automated and manual approaches to determine the extent and spatial configuration of shrubland habitat and recent forest clearcuts. We focused on the state of Rhode Island because (a) it is representative of the northeastern US in terms of the prevalence of private ownership of forests, and the ongoing decline in the populations of many shrubland wildlife species; (b) federal, state and private conservation groups are actively promoting clearcuts to create shrubland habitat; (c) many state-wide GIS databases are available; and (d) the spatial extent of the state made our results both generalizable and politically relevant. Our fine-scale mapping allowed a detailed analysis of shrubland distribution in conjunction with other available GIS layers that facilitates identification of priority areas for habitat management. We found that the extent of upland shrubland in non-coastal areas is decreasing by at least 1.5% annually. Considering the lack of consensus about conservation targets for the amount of shrubland, we propose that conservation organizations attempt to stabilize rather than expand the extent of shrubland habitat. This approach would provide an opportunity to assess whether the current extent of shrubland is sufficient to maintain reduced but stable wildlife populations that require this habitat. We propose a coordinated forest management program with targets for increased forest management on conservation lands. We found that the average patch size of shrubland created by recent clearcuts is large enough for most shrubland bird species, but too small for the New England cottontail (Sylvilagus transitionalis), which has been proposed for threatened and endangered status.  相似文献   

BIOLOGICAL ABSTRACTS     

Osborn H  Hitchens AP  Hooker DR  Kofoid CA  Lewis IF  Ball ED  McClung CE  Schramm JR  Woods AF  Greenman MJ 《Science (New York, N.Y.)》1924,60(1561):485-489

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Neuroscience. Crossing the midline     

Woods CG 《Science (New York, N.Y.)》2004,304(5676):1455-1456

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THE NON-IDENTITY OF "PURE" AND "ISOELECTRIC" GELATINS     

Williams RJ  Friedman L  Woods DM 《Science (New York, N.Y.)》1932,75(1937):199-200

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DEER AS CARRIERS OF ANAPLASMOSIS     

Boynton WH  Woods GM 《Science (New York, N.Y.)》1933,78(2033):559-560

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