Virulence factors in Escherichia coli strains isolated from urinary tract infection and pyometra cases and from feces of healthy dogs     

Siqueira AK  Ribeiro MG  Leite Dda S  Tiba MR  Moura Cd  Lopes MD  Prestes NC  Salerno T  Silva AV 《Research in veterinary science》2009,86(2):206-210

The aim of this study was to compare the prevalence of virulence genes in 158 Escherichia coli strains isolated from 51 clinical cases of UTIs, 52 of pyometra and from 55 fecal samples from healthy dogs by PCR. papC was found in 12 (23.5%) strains isolated from UTIs, 19 (36.5%) from pyometra and 10 (18.2%) from feces. papGII was observed in 3 (5.8%) strains from pyometra, and papGIII in 10 (19.6%) from UTIs, 15 (28.8%) from pyometra and 9 (16.4%) from feces. sfaS was detected in 22 (43.1%) strains from UTIs, 24 (46.1%) from pyometra and 19 (34.5%) from feces. hlyA was observed in 17 (33.3%) strains from UTIs, 18 (34.6%) from pyometra and 7 (12.7%) from feces, while cnf-1 was detected in 11 (21.6%) from UTIs, 21 (40.4%) from pyometra and 9 (16.4%) from feces. iucD was observed in 12 (23.5%) strains from UTIs, 9 (17.3%) from pyometra and 1 (1.8%) from feces. usp was found 17 (33.3%) isolates from UTIs and 36 (69.9%) from pyometra.  相似文献   

Type 2 heat-labile enterotoxin (LT-II)-producing Escherichia coli isolated from ostriches with diarrhea     

Nardi AR  Salvadori MR  Coswig LT  Gatti MS  Leite DS  Valadares GF  Neto MG  Shocken-Iturrino RP  Blanco JE  Yano T 《Veterinary microbiology》2005,105(3-4):245-249

The culture supernatant of Escherichia coli, isolated from ostriches with diarrhea in Brazil, caused elongation in Vero cell, rounding in Chinese hamster ovary (CHO) cells and a cytoplasmic vacuolation in ostrich embryo fibroblasts (OEF), but it was not cytotoxic for chicken embryo fibroblasts (CEF). These effects were not neutralized by antiserum to cholera toxin. Polymerase chain reaction assays showed that the ostrich E. coli contained the gene encoding (eltII-A), but not those for type 1 heat-labile enterotoxin (eltA), heat-stable enterotoxins (estA, estB), verocytotoxins (stx-I, stx-II), or cytotoxic necrotizing factors (cnf 1, cnf 2). All isolates belonged to serotype O15:H8. The enteropathogenic relevance of LT-II in ostrich diarrhea remains undetermined.  相似文献   

Investigation of putative CDT gene in Escherichia coli isolates from pigs with diarrhea     

da Silva AS  da Silva Leite D 《Veterinary microbiology》2002,89(2-3):195-199

In this study, 98 Escherichia coli isolates from 42 diarrheic neonatal piglets were screened for the presence of cytolethal distending toxin coding gene by polymerase chain reaction (PCR). PCR yielded a single product which was specifically generated for E. coli cdt(+) control strain and not for other control strains. Twenty two (22.4%) of the isolates tested were cdtB positive, and 50% of the cdtB(+) isolates were also estII positive. The most prevalent pathotype was O32 cdtB(+) estII(+), which accounted for 59% of the cdtB positive strains. These results indicate an association between the presence of the cdtB gene and diarrhea, and support the need for further studies to determine the role of this toxin in diarrhea.  相似文献   

Partial characterization of the extracellular matrix released onto hydrophobic surfaces by conidia and conidial germlings ofColletotrichum graminicola     

J.A Sugui  B Leite  R.L Nicholson 《Physiological and Molecular Plant Pathology》1998,52(6):411-425

The extracellular matrix (ECM) released by conidia ofColletotrichum graminicolawhen incubated on artificial hydrophobic substrata was investigated. Time-course experiments revealed that proteins and carbohydrates were released in the ECM of both ungerminated and germinated conidia. Stains for protein and carbohydrate revealed that part of the ECM remained attached to the substratum after the fungus had been removed. The ECM isolated from substrata where ungerminated conidia had adhered and from substrata where germinated conidia had adhered exhibited the same profiles when analysed by SDS-PAGE for proteins and glycoproteins and by HPLC for carbohydrate analysis. The ECM was found to be composed of glycoproteins with a molecular weight of around 200 kDa. Deglycosylation of the isolated ECM material resulted in the release of a protein with a molecular weight of approx. 116 kDa, indicating that the original protein was extensively glycosylated. Carbohydrate analysis demonstrated that mannose was the predominant sugar. That the ECM remained attached to the surface of the hydrophobic substrata suggests that it has a role in adhesion.  相似文献   

Comparative study of the microbial profile from bilateral canine otitis externa     

Oliveira LC  Leite CA  Brilhante RS  Carvalho CB 《The Canadian veterinary journal. La revue veterinaire canadienne》2008,49(8):785-788

Fifty dogs with bilateral otitis externa were studied over a 10-month period. The exudates of both external ears were obtained, using sterile swabs, and microorganisms were isolated according to standard microbiological techniques. Antimicrobial susceptibility testing of Staphylococcus intermedius was done by the agar diffusion method. There was bacterial and/or fungal growth in all of the samples. These were all polymicrobial infections. Anaerobic bacteria were not isolated in any sample. The most common pathogens isolated were S. intermedius and Malassezia pachydermatis. A statistically significant difference (P < 0.05) was observed in the isolation pattern between the right and left ears in 34 of the 50 animals (68%). High resistance rates of S. intermedius strains to penicillin, ampicillin, erythromycin, tetracycline, and clindamycin were found. The results suggest that in bilateral canine otitis externa, each ear should be cultured separately and considered as separate units.  相似文献   

Note: Flight capacity,parasitism and emergence of five<Emphasis Type="Italic">Trichogramma</Emphasis> (Hymenoptera: Trichogrammatidae) species from forest areas in Brazil     

M. A. Soares  G. L. D. Leite  J. C. Zanuncio  S. L. Rocha  V. G. M. de Sá  J. E. Serrão 《Phytoparasitica》2007,35(3):314-318

The flight and parasitism capacities and emergence rate of fiveTrichogramma species [T. acacioi (Brun),T. bruni (Nagaraja),T. demoraesi (Nagaraja),T. maxacalii (Voegelé & Pointel) andT. soaresi (Nagaraja)] were compared. The flight capacity of these parasitoids was assessed in test units. A tube was placed inside each test unit with a piece of card with 300Anagasta kuehniella (Zeller) (Lepidoptera: Pyralidae) eggs parasitized by one of theTrichogramma species. The emerged adults inside the test units were classified according to their position as flyers, non-flyers and walkers. Cards withA. kuehniella eggs, parasitized by one of theTrichogramma species, were assessed to determine the parasitism rate and emergence of these natural enemies. The data were submitted to analysis of variance and the means compared by the Scott-Knott test at 5% probability.Trichogramma maxacalii exhibited better flight capacity than the other species, in addition to good parasitism rate and high percentage of emergence. http://www.phytoparasitica.org posting May 10, 2007.  相似文献   

Parasitic helminth infection in young cattle raised on silvopasture and open-pasture in Southeastern Brazil     

R. M. A. de Mendonca  R. C. Leite  A. M. Q. Lana  J. O. Costa  G. Toth 《Agroforestry Systems》2014,88(1):53-62

More than 95 % of Brazil’s beef production occurs in pastures where parasitic helminths are a serious problem, potentially inhibiting an average annual weight gain of 8 kg per young animal. Current pharmaceutical treatments for parasitic infestation are expensive (estimated to cost Brazil 370 million U.S. dollars per year) and sometimes inefficient due to rapid adaptation of worms to new drugs. The presence of trees in pastures (silvopasture) has proven to be environmentally beneficial, but may favor multiplication of parasites and cause higher infestation rates compared to traditional pastures. This study in Southeastern Brazil compared the weight gains of crossbred Holstein and Gir (Zebu) bovines, 8–24 months of age, under two different grazing systems: open (tree-less) pasture, and silvopasture (primarily “Sucupiras Brancas” at 156 trees ha^?1). Despite the favorable environment for helminths, silvopasture did not increase the worm egg count per gram of animal feces and did not create overall weight or weight gain patterns different from those of animals grazing on the open pasture. The experiment took place from August 2008 to February 2009, encapsulating the transition from the dry to rainy season; a period when worm infection rates are highest, causing the greatest impact in animal development. The significance of this period aside, the results must be taken as indicative due to the short time frame and the extraneous challenges of climate variation, individual immune system resistance development, and natural helminth predator growth conditions.  相似文献   

Effects of submergence in water on seed germination and vigor of the Copaifera lucens （Fabaceae） seedlings     

Daniela Baldez Vidal  ;Isis Leite Andrade  ;Eusinia Louzada Pereira Andrade  ;Marcelo Schramm Mielke 《林业研究》2014,25(4):903-908

We analyzed the tolerance of Copaifera lucens seeds to sub- mersion in water to assess the use of this species for direct seeding in riparian forest restoration programs. Seeds were submerged in water for 2, 4, 8, 16 and 32 days or not submerged （control = 0 days of submer- gence）. For the control and at the end of each period of submersion, germination and seedling vigor tests were carried out. For germination tests, seeds were sown in plastic pots containing sand and kept in labora- tory conditions. The percentage of seed germination, the germination rate and the average germination time were analyzed. For seedlings, total biomass, leaf area, leaf mass per area and leaf area ratio were analyzed. Submersion time drastically affected the dissolved oxygen content and seed germination. Between 4 and 8 days of submersion there was a de- crease from 83.8% to 15.6% in the germination percentage. No seed germination occurred after 16 days of submersion. Although there was a significant decrease in the percentage of seed germination between 4 and 8 days of submersion, seedling vigor was not affected. Seeds of this species were partially tolerant to submersion in water, suggesting that C. lucens is a promising species for direct seeding in riparian forest restora- tion projects.  相似文献   

Histomorphometrical study on the skin of different regions of the Caribbean common opossum (Didelphis marsupialis Linnaeus, 1758)     

Reda Mohamed  William Pérez  Marcelo Leite da Veiga 《Anatomia, histologia, embryologia》2021,50(1):144-150

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Tantalum Dissolution Rate in Simulant Lung Fluid     

C. Lima  M. J. Coelho  K. C. P. Dalia  C. V. Barros Leite  G. Medeiros  K. Dias da Cunha 《Water, air, and soil pollution》2007,186(1-4):365-371

The aim of this study is to determine the solubility rate of tantalum contained in pyrochlore, columbite–tantalite and columbite in simulant lung fluid. The International Commission on Radiological Protection (ICRP), in its recommendation for limiting intakes of radionuclide by workers, has consistently recognized that the biological behavior of any specific material after incorporation can significantly diverge from model prevision. Model parameters should be adjusted to adapt the model for each specific substance material in order to estimate the dose due to this element intake. The most recent ICRP publication 66, respiratory tract dosimetry model, point out that information as data like particle size, aerosols solubility and the material chemical compounds are important parameters in the dose coefficients calculation. This paper studies the solubility in Simulant Lung Fluid (SLF) of Ta present in mineral dust particles. For this study 3 minerals were selected: pyrochlore, columbite–tantalite and columbite. Tantalum dissolution in vitro samples were obtained using the Gamble solution and PIXE (Particle Induced X-ray Emission) as analytical technique. In order to characterize the worker exposure to Ta bearing particles, one Brazilian niobium mine was selected. The mineral dust particles were collected using a six-stage cascade impactor and the elemental mass concentrations and the MMAD (Mass Median Aerodynamic Diameter) were determined. Concentrations of radionuclides from natural series of thorium and uranium were also determined. The results show that the workers are exposed to Ta bearing particles in the respirable fraction of aerosols (aerodynamic diameter (d _aer) <2.5 μm) during the mineral processing to obtain Fe–Nb alloy. The solubility in Simulant Lung Fluid (SLF) of Ta present in mineral dust particles depends on the mineral characteristics. The solubility half-time varies between 34 and 62 h depending on the associated mineral.  相似文献