Effect of some viticultural parameters on the grape carotenoid profile     

Oliveira C  Ferreira AC  Costa P  Guerra J  Guedes De Pinho P 《Journal of agricultural and food chemistry》2004,52(13):4178-4184

The effect of some viticultural parameters on the grape carotenoid profile was investigated. Grape cultivar, ripeness stage, sunlight and shade exposure, altitude, and vegetative height were studied. Differences between cultivars were observed in eight different black grape varieties: Touriga Brasileira (TBR), Tinta Barroca (TB), Tinta Amarela (TA), Souz?o (S), Touriga Franca (TF), Touriga Nacional (TN), Tinta Roriz (TR), and Tinto C?o (TC), from the Douro region. TA and TBR clearly produced higher concentrations of carotenoids. Results showed that carotenoid content decreased during ripening. Decreases of lutein were observed until 66%, whereas beta-carotene slowly decreased, having a constant level until the harvest date. Carotenoid contents were consistently higher in grapes exposed to shade than in those exposed to direct sunlight in both studied white grape varieties, Maria Gomes (MG) and Loureiro (L). In the Douro Valley, high-elevation terraces, which presented a lower temperature and higher humidity during the maturation period, appeared to produce grapes with higher carotenoid values. Grapes grown with higher vegetative height seem to have higher carotenoid levels; furthermore, grapes grown with lower vegetative height had higher weight and sugar concentrations.  相似文献   

IgG isotype profile is correlated with cardiomegaly in Beagle dogs infected with distinct Trypanosoma cruzi strains     

Guedes PM  Veloso VM  Gollob KJ  Afonso LC  Caldas IS  Vianna P  de Lana M  Chiari E  Bahia MT  Galvão LM 《Veterinary immunology and immunopathology》2008,124(1-2):163-168

A systematic study following infection by various strains of the protozoan parasite, Trypanosoma cruzi, and the simultaneous monitoring of the humoral immune response together with the elicited cellular response, could add greatly to our understanding of differences between strains of this important human pathogen. In that sense, acute and chronic infections with distinct T. cruzi strains (Y, Berenice-78 and ABC) in Beagle dogs were studied through a longitudinal evaluation of immunoglobulin G (IgG), IgG1 and IgG2 isotypes (by ELISA and flow cytometry (FC)), as well as measurements of peripheral blood mononuclear cell (PBMC) proliferation over a 100-week period, and their correlation with cardiomegaly. Our results show that infected animals presenting cardiomegaly showed lower or absent levels of IgG1 during the chronic phase of the infection, when compared to those that did not show an increase in heart weight. In that manner, our results suggest that IgG1 could be used as a marker for cardiac pathogenicity in Chagas disease.  相似文献   

Occurrence and genotype characterization of Giardia duodenalis in goat kids from the Canary Islands, Spain     

Ruiz A  Foronda P  González JF  Guedes A  Abreu-Acosta N  Molina JM  Valladares B 《Veterinary parasitology》2008,154(1-2):137-141

Giardia duodenalis (syn. Giardia lamblia, Giardia intestinalis) is a wide-spread intestinal protozoa of both humans and animals. Although giardiosis in goat is commonly asymptomatic, young kids may bear an enteric disease associated with persistent diarrhoea and delayed weight gain. In the present study we have analysed the occurrence of Giardia in 315 young goat kids (2–6 months old) from Gran Canaria Island (Spain) through visualization of faecal cysts. The identification of genotypes of G. duodenalis among the farms was attained by nested PCR of the triophosphate isomerase (TPI) and single PCR of β-giardin genes and subsequent sequencing. Positive samples were found in 42.2% of the animals and 95.5% of the farms. Goat faecal specimens were positive for only livestock-associated G. duodenalis assemblage E genotype for both TPI and β-giardin genes. The genetic analysis of these two loci revealed the presence of different haplotypes among the farms included in the survey and high homology with homologous genes from cattle and sheep. Altogether, the data presented here provide additional information to the prevalence and genetic characterization of Giardia isolates. The absence of assemblages A and B in this study suggests that zoonotic transmission of Giardia from goats could be of low epidemiological significance, although these findings should be validated in studies including other geographical areas, age groups and larger number of samples.  相似文献   

Transversus abdominis plane block in ponies: a preliminary anatomical study     

Caroline F. Baldo  Daniel Almeida  Erin Wendt-Hornickle  Alonso Guedes 《Veterinary anaesthesia and analgesia》2018,45(3):392-396

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In vitro antimicrobial activity against equine Lawsonia intracellularis strains     

C. E. R. Pereira  T. P. Resende  E. Vasquez  L. Marshall-Lund  R. M. C. Guedes  C. J. Gebhart 《Equine veterinary journal》2019,51(5):665-668

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Pharmacokinetics and antinociceptive effects of the soluble epoxide hydrolase inhibitor t‐TUCB in horses with experimentally induced radiocarpal synovitis          下载免费PDF全文

A. G. P. Guedes  F. Aristizabal  A. Sole  A. Adedeji  R. Brosnan  H. Knych  J. Yang  S.‐H. Hwang  C. Morisseau  B. D. Hammock 《Journal of veterinary pharmacology and therapeutics》2018,41(2):230-238

This study determined the pharmacokinetics, antinociceptive, and anti‐inflammatory effects of the soluble epoxide hydrolase (sEH ) inhibitor t ‐TUCB (trans ‐4‐{4‐[3‐(4‐Trifluoromethoxy‐phenyl)‐ureido]‐cyclohexyloxy}‐benzoic acid) in horses with lipopolysaccharide (LPS )‐induced radiocarpal synovitis. A total of seven adult healthy mares (n  = 4–6/treatment) were administered 3 μg LPS into one radiocarpal joint and t ‐TUCB intravenously (i.v.) at 0 (control), 0.03, 0.1, 0.3, and 1 mg/kg in a blinded, randomized, crossover design with at least 3 weeks washout between. Two investigators independently assigned pain scores (at rest, walk and trot) and lameness scores before and up to 48 hr after t ‐TUCB /LPS . Responses to touching the joint skin to assess tactile allodynia, plasma, and synovial fluid (SF ) t ‐TUCB concentrations were determined before and up to 48 hr after t ‐TUCB /LPS . Blood and SF were collected for clinical laboratory evaluations before and up to 48 hr after t ‐TUCB /LPS . Areas under the curves of pain and lameness scores were calculated and compared between control and treatments. Data were analyzed using repeated measures ANOVA with Dunnett or Bonferroni post‐test. p  < .05 was considered significant. Data are mean ± SEM . Compared to control, pain, lameness, and tactile allodynia were significantly lower with 1 mg/kg t ‐TUCB , but not the other doses. For 0.1, 0.3, and 1 mg/kg t ‐TUCB treatments, plasma terminal half‐lives were 13 ± 3, 13 ± 0.5, and 24 ± 5 hr, and clearances were 68 ± 15, 48 ± 5, and 14 ± 1 ml hr^?1 kg^?1. The 1 mg/kg t ‐TUCB reached the SF at high concentrations. There were no important anti‐inflammatory effects. In conclusion, sEH inhibition with t ‐TUCB may provide analgesia in horses with inflammatory joint pain.  相似文献   

Further insights into the role of methional and phenylacetaldehyde in lager beer flavor stability   总被引：2，自引：0，他引：2  

Soares da Costa M  Gonçalves C  Ferreira A  Ibsen C  Guedes de Pinho P  Silva Ferreira AC 《Journal of agricultural and food chemistry》2004,52(26):7911-7917

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Influence of Delipidation on Hg Analyses in Biological Tissues: A Case Study for an Antarctic Ecosystem     

Caio V. Z. Cipro  Paco Bustamante  Rosalinda C. Montone 《Water, air, and soil pollution》2017,228(5):188

The use of low-polarity organic solvents is widespread in cleanup/extraction processes in order to carry compounds of interest, remove interferences and separate phases, among other uses. A large number of studies have used delipidation to remove excess of lipids to analyse carbon stable isotopes in biological tissues for trophic and behavioural ecology investigations. In this context, the primary aim of this study is to assess the influence of one delipidation process on the results of total mercury (Hg) analyses and the possible use of delipidated samples from previous analyses, such as for stable isotopes, in Hg level determination. Samples of vegetation (angiosperm, lichens and mosses), invertebrates (krill and limpets), fish (marbled and black rockcod), bird liver and eggs (Antarctic, Gentoo and Adélie penguins, kelp gull, Antarctic tern, cape petrel and giant southern petrel) and pinniped tissues (Weddell seal, crabeater seal, southern elephant seal and Antarctic fur seal) were analysed for Hg before and after delipidation by cyclohexane. The difference between the two measurements ranged individually from ?63 to 136% (in the moss Sanionia uncinata) and the averages ranged from ?60 to 66% (in pinniped tissues). The proportion of organic Hg, which presents considerable lipophilicity, but also high affinity for sulfhydryl groups in proteins, might be responsible for such variability. Given the limitations of our study, we think it is safe to say that delipidated samples could not be used to infer total Hg values in non-delipidated ones.  相似文献   

Computed tomography diagnosis of eight dogs with brain infarction     

AEH Paul  Z Lenard  CS Mansfield 《Australian veterinary journal》2010,88(10):374-380

Objective Medical records of eight dogs presenting with acute onset of neurological signs and a diagnosis of brain infarction as determined by computed tomography (CT) imaging were reviewed. Design Retrospective single-centre case review Results Ischaemic infarction in the territory of the rostral cerebellar artery was identified in three spaniel-breed dogs. All cerebellar infarcts were non-haemorrhagic. Telencephalic infarcts were identified in five dogs, in the territories of the middle cerebral artery (2/5) and rostral cerebral artery (3/5). One of these dogs had an ischaemic infarction, but all other infarctions appeared haemorrhagic. All dogs were geriatric (≥8 years old), with concurrent medical conditions identified in six dogs. One dog was euthanased after diagnosis because of the severity of its neurological signs and one dog was euthanased as a result of associated renal disease 2 months after diagnosis. Six dogs were alive at least 1 year after diagnosis. Conclusions CT is useful in the diagnosis of cerebrovascular accident in dogs, which can present as a spectrum of images with early changes in attenuation and subtle mass effects detected after infarction. CT is particularly sensitive for detecting haemorrhagic infarction, but under-represent ischaemic and lacunar infarctions when compared with MRI.  相似文献   

Designing artificial anterior cruciate ligaments based on novel fibrous structures     

J. Cruz  S. Rana  R. Fangueiro  R. Guedes 《Fibers and Polymers》2014,15(1):181-186

The present paper reports the development of novel braided structures using polyamide 6.6 fibers for application as artificial anterior cruciate ligaments (ACLs). The developed structures were circular braids, axially reinforced with either a number of core yarns or braided structures. Tensile behavior of these structures was characterized and the effects of number of axial yarns or braids and, the number of yarns used in the axial braids were thoroughly investigated. From the experimental results, it was observed that the braided structures with axial braids could mimic the load-elongation behaviour of native ACL. The average breaking extension and strain at toe region were 30 % and 4.3 % respectively, which are in the range of native ACLs. The maximum breaking force and stiffness achieved with 7 axial braids, each produced using 6 yarns were 274 N and 13.5 N/mm respectively and, both breaking force and stiffess showed linear increase with the number of axial braids as well as number of yarns used in the axial braids. Therefore, it is possible to design an artificial graft using these novel braided sturctures with mechanical properties similar to that of native ACLs, through adjustment of these structural parameters, as these braided structures have much smaller diameter (0.5 mm) than native ACLs (11 mm).  相似文献