排序方式： 共有85条查询结果，搜索用时 15 毫秒
11.
Carine de Freitas Souza Matheus Dellaméa Baldissera Adriane Erbice Bianchini Elisia Gomes da Silva Rosa Helena Veraz Mourão Lenise Vargas Flores da Silva Denise Schmidt Berta Maria Heinzmann Bernardo Baldisserotto 《Fish physiology and biochemistry》2018,44(1):21-34
The viability using Lippia alba essential oil as an anesthetic for fish was studied, particularly with respect to physiological effects during recovery. Anesthesia of silver catfish (Rhamdia quelen) using 100 and 300 μL L?1 of two different chemotypes of L. alba essential oil (citral EO-C and linalool EO-L) prevented the increase of plasma cortisol levels caused by handling, but did not avoid alterations in energetic metabolism. Silver catfish did not have increased the levels of thiobarbituric acid reactive species in the kidney and liver during recovery after anesthesia with either EO, avoiding lipid damage. On the other hand, fish anesthetized with EO-C showed higher protein carbonylation levels, superoxide dismutase, catalase, and glutathione S-transferase activities and non-protein thiol group levels in both tissues compared to controls. Our results suggest that both oils show antioxidant capacity, but anesthesia with EO-L does not cause damage to lipids or proteins, only temporary changes, typical of physiological adjustments during recovery from anesthesia. Therefore, EO-L is an effective anesthetic for silver catfish with fewer side effects than EO-C. 相似文献
12.
Janaína?Camacho?da?Silva Antonio?Sergio?Varela Junior J?sie?Shwartz?Caldas Clarissa?da?Silva Freitas Joziel?Gon?alves?Botelho Elton?Pinto?Colares Carine?Dahl?CorciniEmail authorView author&#;s OrcID profile 《Fish physiology and biochemistry》2016,42(1):93-102
Sperm quality tests on fish are classically used for evaluating cryopreservation procedures, and they are also promising to assess aquatic toxicity and biomarkers of xenobiotic effects on reproduction. Osmotic shock from the storage medium is one of the main factors affecting sperm quality during evaluation. Thus, the objective of this study was to evaluate the effects of different osmolalities (240–460 mOsm/kg) for at least 4 days on the sperm quality parameters of the viviparous fish Jenynsia multidentata. The level of significance was (P < 0.05). The plasma osmolality of J. multidentata is 326 ± 3.9 mOsm/kg. The motility of fresh semen was higher in osmolalities of 280 and 300 mOsm/kg but did not differ between osmolalities from 240 to 320 mOsm/kg. Above 380 mOsm/kg, the motility observed was 0 %. Over the time period studied motility increased with increasing osmolality, and the most constant and long-lasting rates were between 300 and 320 mOsm/kg. On the 4th day of evaluation, higher membrane integrity rates were observed between 280 and 360 mOsm/kg, higher mitochondrial membrane potential was observed between 300 and 460 mOsm/kg, and higher DNA integrity rates were observed between 260 and 380 mOsm/kg. Moreover, osmolalities ≥460 and ≤240 resulted in the lowest motility and DNA integrity levels. Over 4 days, the plasma membrane integrity was significantly lower at ≤260 and ≥400 mOsm/kg, and the mitochondrial membrane potential was significantly lower only in osmolalities ≤240 mOsm/kg. Therefore, we conclude that for sperm quality preservation in J. multidentata, an osmolality of 300–320 mOsm/kg of the most suitable diluent is necessary. Furthermore, we conclude that the storage of sperm in a hyposmotic (<260 mOsm/kg) or hyperosmotic (>400 mOsm/kg) solution affects not only motility but also other sperm quality parameters. 相似文献
13.
Cesar García-Hernández Javier Arece-García Rolando Rojo-Rubio German David Mendoza-Martínez Benito Albarrán-Portillo José Fernando Vázquez-Armijo Leonel Avendaño-Reyes Agustín Olmedo-Juárez Carine Marie-Magdeleine Yoel López-Leyva 《Tropical animal health and production》2017,49(1):55-61
Forty-five Pelibuey sheep were experimentally infested with nematodes to evaluate the effect of three free condensed tannin (FCT) levels of Lysiloma acapulcensis on fecal egg counts (FECs), packed cell volumes (PCV), ocular mucosa colors (OMC), average daily gain (ADG), and adult nematode count. Five treatments were used: 12.5, 25.0, and 37.5 mg of FCT kg?1 of body weight (BW); sterile water (control); and ivermectine (0.22 mg kg?1 of BW) as chemical group. The data were processed through repeated measurement analysis. Even though the three FCT doses decreased (P?<?0.05) the FEC, the highest reduction was obtained with 37.5 mg kg?1 of BW. No differences were observed in PCV and OMC. Higher ADG (P?<?0.05) was observed with 37.5 mg kg?1 of BW of FCT. The count of adult nematodes (females and males) in the higher dose of FCT was similar to chemical treatment. Dose of 37.5 mg kg?1 of BW decreased the parasite infection and improved the lamb performance. Therefore, this dose could be used as a nutraceutic product in sheep production. 相似文献
14.
Diogenes Dezen Franciscus Antonius Maria Rijsewijk Thais Fumaco Teixeira Carine Lidiane Holz Samuel Paulo Cibulski Ana Cláudia Franco Odir Antonio Dellagostin Paulo M. Roehe 《Research in veterinary science》2010,88(3):436-440
Multiply-primed rolling-circle amplification (MPRCA) was used to amplify porcine circovirus type 2 (PCV2) genomes isolated from tissues of pigs with signs of post-weaning multisystemic wasting syndrome (PMWS). Two of the amplified PCV2 genomes were cloned in prokaryotic plasmids and sequenced. Both were nearly identical (1767 nt) except for one silent substitution in the region coding for the capsid protein (ORF2). In addition, they showed high nucleotide sequence similarity with PCV2 isolates from others countries (93–99%). To investigate whether the MPRCA amplified PCV2 genomes could be used to produce infectious virus, the cloned genomes were isolated from the plasmids, recircularized and used for transfection in PK-15 cells. This procedure led to the production of infectious virus to titres up to 105.55 TCID50/mL. It was concluded that MPRCA is a useful tool to amplify PCV2 genomes aiming at sequencing and virus isolation strategies, where particularly useful is the fact that it allows straightforward construction of PCV2 infectious clones from amplified genomes. However, it was less sensitive than PCR for diagnostic purposes. 相似文献
15.
Laureyns J Pardon B Letellier C Deprez P 《The Canadian veterinary journal. La revue veterinaire canadienne》2011,52(10):1135-1139
After 3 cows of a dairy herd had died from severe hemorrhagic diarrhea, a 4th sick cow was transported to the clinic. Blood analyses revealed the complete absence of white blood cells, the presence of a type 1b strain of bovine viral diarrhea virus (BVDV), and seroconversion to BVDV. 相似文献
16.
Salvatori M. De Groeve J. van Loon E. De Baets B. Morellet N. Focardi S. Bonnot N. C. Gehr B. Griggio M. Heurich M. Kroeschel M. Licoppe A. Moorcroft P. Pedrotti L. Signer J. Van de Weghe N. Cagnacci F. 《Landscape Ecology》2022,37(5):1453-1468
Landscape Ecology - Diel use of forest and open habitats by large herbivores is linked to species-specific needs of multiple and heterogeneous resources. However, forest cover layers might deviate... 相似文献
17.
Bruno Vincent Fotini Koutroumpa Valérie Altemayer Géraldine Roux-Morabito Jeremy Gevar Carine Martin François Lieutier 《Annals of Forest Science》2008,65(1):111-111
As a consequence of the recent introduction of the pine wood nematode Bursaphelenchus xylophilus in Portugal, nematodes of the genus Bursaphelenchus were looked for in various French pine forests, in trees attacked by Monochamus galloprovincialis, the vector insect of B. xylophilus, and in the insects themselves. Trap trees were felled in 12 localities distributed all over the country. Nematodes were extracted from transversal stem discs; insects emerging from the trap trees were studied. B. hellenicus, B. leoni, B. mucronatus and B. sexdentati were isolated, but not B. xylophilus. The presence of B. mucronatus and the absence of B. xylophilus were confirmed by molecular markers. B. mucronatus was isolated from several regions with an average prevalence of 19%. The infestation of M. galloprovincialis by B. mucronatus reached 26.7%. The wide distribution of B. mucronatus in France could have an effect on the extension of B. xylophilus in a case of an introduction. 相似文献
18.
19.
Carine K. Souza Daiane P. Oldiges Ana Paula S. Poeta Itabajara da S. Vaz Rejane Schaefer Danielle Gava Janice R. Ciacci‐Zanella Cludio W. Canal Luís G. Corbellini 《Zoonoses and public health》2019,66(1):125-132
Backyard pig populations are not monitored for influenza A virus (IAV) in Brazil and there are limited data about seroprevalence and risk factors in these populations. Our goal was to assess possible factors associated with IAV seroprevalence in backyard pig populations using an indirect ELISA protocol based on a recombinant nucleoprotein. Following the IAV screening using NP‐ELISA, subtype‐specific serology based on hemagglutination inhibition (HI) assay of the ELISA‐positive pigs was conducted. The survey comprised a total of 1,667 sera samples collected in 2012 and 2014 in 479 holdings and the estimated seroprevalence was 5.3% (3.84%–7.33%) and 2.3% (1.34%–3.71%) in the respective years. In both years, H1N1pdm09 was the most prevalent subtype. The multivariable analysis showed main factors such as “age,” “sex,” “number of suckling pigs” and “neighbours raising pigs” that presented the greatest effect on IAV seroprevalence in these pig populations. These factors may be associated with the low biosecurity measures and management of backyard holdings. In addition, the low IAV seroprevalences found in these backyard pig populations could be related to a low number of animals in each pig holding and low animal movement/replacement that do not favour IAV transmission dynamics. This low frequency of H1N1pdm09 seropositive pigs could also be due to sporadic human‐to‐pig transmission of what is now a human seasonal influenza A virus; however, these factors should be explored in future studies. Herein, these results highlight the importance of IAV continued surveillance in backyard pig holdings, since it is poorly known which IAVs are circulating in these populations and the risk they could pose to public health and virus transmission to commercial farms. 相似文献
20.
Tnia C. Leite Fernanda Picoli Diogo de A. Lopes Matheus D. Baldissera Carine F. Souza Bernardo Baldisserotto Ana Paula O. Costa Luisa Nora Alexandre H. Marcelino Aleksandro S. Da Silva 《Aquaculture Research》2021,52(1):395-402
The aim of the present study was to determine whether açai oil (Euterpe oleraceae) addition in the diets of juvenile Nile tilapia (Oreochromis niloticus) in biofloc system would stimulate hepatic energy metabolism and antioxidant responses. Treatments are as follows: T0 (6%‐soybean oil), T1 (1%‐AÇAI; 5%‐SOY), T3 (3% AÇAI; 3%‐SOY) and T6 (6%‐AÇAI). In the liver, reactive oxygen species levels were significantly lower in T1, T3 and T6 than in T0, lipid peroxidation levels were significantly lower in T3 and T6, and nitric oxide levels were significantly lower in the T6 than in T0. Glutathione peroxidase activity was significantly higher in the T3 and T6 than in T0, while superoxide dismutase activity and total antioxidant capacity levels were significantly higher in the T6 than in T0. Adenylate kinase activity was significantly lower in the T6 than in T0. These results suggest that the açai oil addition in Nile tilapia diets stimulated antioxidant responses. 相似文献