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71.
Carine Rizzolatti Paul Bury Edouard Tatara Pierre A. Pin Nathalie Rodde Hélène Bergès Françoise Budar Hakim Mireau Jan J. L. Gielen 《Euphytica》2017,213(12):276
Hybridization technology has proven valuable in enhancing yields in many crops, but was only recently adopted in the small grain cereals. Hybrid varieties in barley (Hordeum vulgare) rely on the cytoplasmic male sterility (CMS) system msm1 derived from Hordeum vulgare ssp. spontaneum. The major restorer gene described for the msm1 system is known as Rfm1 and maps to the top of chromosome 6H. To gain further insight into mechanisms underlying male fertility restoration in barley, we used a map-based cloning approach to identify the nuclear gene involved in the restoration mechanism of this hybridization system. Taking advantage of the available genomic resources in barley in combination with a custom-made non-gridded BAC library developed from a restorer line, we cloned and sequenced the Rfm1 restorer locus. The characterization and annotation of the nucleotide sequence for the Rfm1 restorer allele allowed for the identification of the candidate gene for Rfm1. The Rfm1 locus carries a tandem repeat of a gene encoding a pentatricopeptide repeat (PPR) protein. Surprisingly, Rfm1 belongs to the PLS-DYW subfamily of PPR genes known for their involvement in RNA editing in plants organelles, but that to date have not been identified as restorer genes. 相似文献
72.
Jane Mello Lopes Carine de Freitas Souza Etiane Medianeira Hundertmarck Saccol Maria Amlia Pavanato Alfredo Antoniazzi Monique Tomazele Rovani Berta Maria Heinzmann Bernardo Baldisserotto 《Aquaculture Nutrition》2019,25(2):310-318
The effects of dietary supplementation of graded level (0, 0.25, 0.5, 1.0, and 2.0 ml/kg diet) of Citrus aurantium essential oil (EOCA) on the growth, metabolic, and oxidative parameters of silver catfish (Rhamdia quelen) were investigated in a 60‐day growth trial. Fish fed with 2.0 ml EOCA per kg exhibited significantly better growth performance than those fed the control diet. Glucose, lactate, and protein levels in liver and muscle were altered significantly by dietary addition of EOCA. Hepatic lipid peroxidation levels, measured using thiobarbituric acid reactive substance and lipid hydroperoxides assays, were reduced in animals receiving the diet containing EOCA. Superoxide dismutase activity was higher, while glutathione S‐transferase activity was lower in the liver of fish receiving 0.5, 1.0, and 2.0 ml EOCA per kg of diet than in control. The nonprotein thiols content was higher in fish receiving the EOCA‐containing diet. Thus, dietary addition of EOCA improved growth, biochemical, and antioxidant parameters in silver catfish and could be useful as dietary supplement. 相似文献
73.
Wassim Habib Carine Saab Robert Malek Louis Kattoura Caterina Rotolo Elvis Gerges Farah Baroudy Stefania Pollastro Francesco Faretra Rita M. De Miccolis Angelini 《Plant pathology》2020,69(8):1453-1468
Tomato and strawberry are the most important protected crops in Lebanon and are seriously affected by grey mould disease, caused by Botrytis cinerea. In the present study, the fungicide sensitivity assays revealed medium to high frequencies of B. cinerea isolates resistant to benzimidazoles, dicarboximides, and anilinopyrimidines on tomato and strawberry. Fludioxonil- and boscalid-resistant mutants were uncommonly found at generally low frequency on both crops. Resistance to fenhexamid was detected in only one site on tomato but in most sites on strawberry with high frequencies, and the occurrence of resistance to QoI fungicides was ascertained on both crops. The majority of the tested isolates (>90%) exhibited multiple fungicide resistance, and isolates resistant to the seven antibotrydial fungicide classes were detected on strawberry in three locations. A high level of resistance was shown by B. cinerea mutants resistant to boscalid, fenhexamid, and QoI fungicides, while two levels of moderate and high resistance to anilinopyrimidines were identified. Genetic analysis revealed point mutations in the target genes commonly associated with resistance in B. cinerea isolates, with all mutants resistant to dicarboximides, fenhexamid, boscalid, and QoI fungicides carrying single-nucleotide polymorphims in BcOS1 (I365S/N, Q369P, and N373S), Erg27 (F412V/I), SdhB (H272R/Y), and cytb (G143A) genes, respectively. The general incorrect use of fungicides has caused the development and spread of fungicide resistance as a widespread phenomenon on protected tomato and strawberry in Lebanon. The implementation of appropriate antiresistance strategies is highly recommended. 相似文献
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75.
Rosaria Cataudella Marina Paolucci Carine Delaunay Anne Ropiquet Alexandre Hassanin Maria Balsamo Frédéric Grandjean 《水产资源保护:海洋与淡水生态系统》2010,20(3):261-268
- 1. Ten populations of Austropotamobius italicus from the Marches region (Central Italy), two populations from the Appennines of Calabria and Basilicata (Southern Italy) and four populations from Friuli Venezia Giulia region were sampled to elucidate the geographical pattern of genetic variability in relation to recent and historical factors.
- 2. A total of nine COI haplotypes belonging to three separate lineages have been characterized. Both lineage A (Northern Marches region) and C (Friuli Venezia Giulia region) were represented by one haplotype and were related to the Istria 1 peninsula and SouthAlps/Western Balkans lineages respectively. The lineage B included seven haplotypes belonging to the Appenines lineage.
- 3. Two hypotheses on the origin of individuals belonging to the Istrian lineage in Marches are discussed: (i) a natural colonization from the Istria peninsula refugia by migrating westwards across the bridge between the Istria peninsula and Italy, (ii) an anthropogenic origin. Based on 16S rRNA sequences, the taxonomic status of lineage B and C, defined from COI mtDNA markers, belongs to A. i. meridionalis and that of lineage A belongs to A. i. carsicus. The existence of a complex geographic structure between Marches populations (Φst=0.66) of A. i. meridionalis should be further considered in conservation management. Copyright © 2010 John Wiley & Sons, Ltd.
76.
Jean Heinrich Daugrois Carine Edon-Jock Sandrine Bonoto Jean Vaillant Philippe Rott 《European journal of plant pathology / European Foundation for Plant Pathology》2011,129(1):71-80
Sugarcane yellow leaf virus, the causal agent of yellow leaf, is transmitted from plant to plant by aphids. Understanding and evaluating the epidemic
risks due to spread of yellow leaf by aphids is an important feature for sugarcane production. Four distinct sugarcane trials
were set up with disease-free plants to study the relationship between spread of yellow leaf, the vector dynamics and environmental
conditions that may favour yellow leaf epidemics. The study was performed by surveys of vector populations and determination
of plant infections. Sugarcane cultivar SP71-6163, highly susceptible to yellow leaf, was analyzed spatially at different
dates in all four trials and compared to commercial cultivars in two of the four trials. These surveys allowed us to identify
a correlation between the aphid dynamics in the field and yellow leaf progress. Additionally, a negative correlation was found
between rainfall during the first weeks after transferring sugarcane plants to the field and aphid dispersal within the field.
This later result revealed an impact of rainfall on aphid invasion and subsequent plant infection by SCYLV. If aphids are
the key factor for disease spread, plant response varied also according to cultivar resistance with high variation depending
on rain conditions. 相似文献
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This study under field experimental conditions in apple orchard agroecosystems investigated the effects of pest management strategies (i.e. none, organic, conventional and integrated) on enzyme activities, in relation to soil properties. Enzyme activities chosen are implicated in the major biogeochemical nutrient cycles such as C (cellulase, fluoresceine diacetate hydrolase, β-galactosidase, β-glucosidase, phenol oxidase), N (arylamidase), P (acid and alkaline phosphomonoesterases, phosphodiesterase and phosphotriesterase) and S (arylsulfatase). Redundancy analyses and decomposition of the variances were performed to clarify how enzyme activities are affected by management strategy and soil properties. Results showed that the effects and their proportion attributable to management strategy and soil properties varied considerably depending on enzyme activity. Phenol oxidase activity was the only case where total variance was principally explained by management strategy (i.e. conventional and integrated) rather than by soil properties, and thus it seems to be an attractive potential indicator to assess soil quality in this agrochemical context. 相似文献
79.
Fotini A. Koutroumpa Bruno Vincent Géraldine Roux-Morabito Carine Martin François Lieutier 《Annals of Forest Science》2008,65(7):707-707
80.
Emmie Dornez Kurt Gebruers Iris J. Joye Bart De Ketelaere Jonathan Lenartz Carine Massaux Bernard Bodson Jan A. Delcour Christophe M. Courtin 《Journal of Cereal Science》2008
The effects of genotype, harvest year and their interaction on the levels of arabinoxylans (AX), endoxylanases and endoxylanase inhibitors in wheat were studied using 14 varieties grown in three successive growing periods with diverse climatological conditions. Relations with more commonly evaluated wheat characteristics such as yield, thousand kernel weight, specific weight, protein level, Hagberg falling number (HFN) and α-amylase activity level were examined. Water extractable arabinoxylan (WE-AX) levels in wheat varied much more than total arabinoxylan (TOT-AX) levels. This variability was mainly genetically determined, but harvest year also had an important effect. Total endoxylanase activity levels varied more than a factor of 20 between the different wheat samples. Endogenous endoxylanases typically accounted for only 10–15% of this activity, while wheat-associated microbial endoxylanases accounted for the remaining 85–90%. However, when preharvest sprouting occurred, the contribution of endogenous endoxylanases could sometimes amount to over 40% of this total activity. Endogenous endoxylanase activity levels were mainly determined by the interaction of genotype and harvest year, while wheat-associated microbial endoxylanase activity levels were predominantly determined by genotype alone. Endogenous and microbial endoxylanase activity levels were strongly correlated, suggesting that wheat varieties which are susceptible to preharvest sprouting are often also susceptible to microbial contamination. The TAXI and XIP-type endoxylanase inhibitor levels varied by a factor of 8 and 1.8, respectively. They were mainly determined by genotype and were rather similar in the different growing periods. 相似文献