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61.
Despite a general consent about the beneficial contribution of arbuscular mycorrhizal fungi (AMF) on natural ecosystems, there is an intense debate about their role in agricultural systems. In this work, soybean (Glycine max L.) and sunflower (Helianthus annuus L.) field plots with different P availabilities were sampled across the Pampean Region of Argentina (> 150 samples from Mollisols) to characterize the relationship between available soil P and indigenous mycorrhizal colonization. A subsequent pot experiment with soybean and sunflower was carried out to evaluate the effect of P supply (0, 12, and 52 mg P kg–1) and AMF inoculation on AMF colonization and crop responsiveness to P in a Mollisol. Both crops showed high AMF colonization in the field (average: 55% for soybean and 44% for sunflower). While mycorrhizal colonization in soybean was significantly and negatively related to available soil P, no such trends were apparent in sunflower. Also, total biomass was 3.5 and 2.0 times higher in mycorrhizal than in nonmycorrhizal pot‐grown soybean under low‐ and medium‐P conditions, respectively. Sunflower, on the other hand, did not benefit from AMF symbiosis under medium and high P supply. While mycorrhization stimulated P‐uptake efficiency in soybean, the generally high P efficiency in sunflower was not associated with AMF symbiosis.  相似文献   
62.
63.
The potato leafroll virus (PLRV) P0 protein (P0PL) is a suppressor of RNA silencing. In this study, we showed that P0 protein from an Argentinian isolate of PLRV (P0PL-Ar) has an additional activity not described for other PLRV or P0 proteins from poleroviruses. Besides reporting that P0PL-Ar displays both local and systemic silencing suppressor activity, we demonstrated, for the first time, that P0PL-Ar impedes accumulation of dsRNA-derived siRNAs. We also showed that P0PL-Ar interacts with Solanum tuberosum SKP1 orthologue (StSKP1) and triggers destabilization of ARGONAUTE 1 (AGO1) and that these actions are mediated by the F-box-like domain. A mutant in the GW/WG motif within the P0PL-Ar F-box-like motif lost the suppression activity, the interaction with StSKP1 and abolished AGO1 decay. Interestingly, a mutant in the L76/P77 residues within the P0PL-Ar F-box-like motif, which lost the suppression activity and the interaction with StSKP1, retained the capacity to enable AGO1 decay. Thus, unlike other P0 proteins of previously characterized poleroviruses, P0PL-Ar seems to have a dual activity, according to the findings of this study. This protein would act at both an upstream and a downstream step of the RNA silencing pathway: upstream of Dicer-like enzyme (DCL)-mediated primary siRNA production and downstream at the RNA-induced silencing complex (RISC) complex level. Our results contribute to the understanding of the different ways PLRV P0 proteins function as silencing suppressors.  相似文献   
64.

Background

Efficient division of reproductive labor is a crucial characteristic of social insects and underlies their ecological and evolutionary success. Despite of the harmonious appearance of insect societies, nestmates may have different interests concerning the partitioning of reproduction among group members. This may lead to conflict about reproductive rights. As yet, few studies have investigated the allocation of reproduction among queens in multi - queen societies ("reproductive skew"). In the ant Leptothorax acervorum, reproductive skew varies considerably among populations. While reproduction is quite equally shared among nestmate queens in most populations from boreal Eurasia (low skew), colonies from populations at the edge of the species' range are characterized by "functional monogyny," i.e., high skew. The proximate mechanisms underlying high skew, in particular how workers influence which queen lays eggs, are not well understood. We investigated the behavior of queens and workers in functionally monogynous colonies of L. acervorum from two mountain ranges in central Spain.

Results

We provide evidence for both queen and worker influence on the outcome of conflict over reproduction in colonies of L. acervorum from Spain. The patterns of queen - queen aggression and worker - queen grooming and feeding after hibernation allowed predicting, which queen later began to lay eggs. In contrast, worker aggression towards queens was not clearly associated with a queen's future reproductive success. Queen - queen and worker - queen aggression differed in quality: queens typically engaged in ritualized dominance behavior, such as antennal boxing, while workers also attacked queens by biting and prolonged pulling on their legs and antennae. In several cases, overt worker aggression led to the expulsion of queens from the nest or their death.

Conclusion

We conclude that queens of L. acervorum from Spain establish rank orders by ritualized dominance interactions, such as antennal boxing. Workers may reinforce these hierarchies by preferentially feeding and grooming high ranking queens and attacking lower ranking queens. Aggressive worker policing may thus stabilize functional monogyny. Optimal skew models predict that high skew in ants is associated with high dispersal costs. In central Spain, L. acervorum is restricted to small patches at higher elevations, which presumably makes dispersal and colony founding difficult. Because of the ecological requirements of L. acervorum and the predicted large impact of global change on central Spain, the functionally monogynous populations of this ant must be considered as threatened.  相似文献   
65.

Precision agriculture (PA) has a huge potential for growth in sub-Saharan Africa (SSA), but it faces a number of social-economic and technological challenges. This study sought to map existing PA research and application in SSA countries following the methodology for systematic mapping in environmental sciences. After screening for relevance, the initial about 7715 articles was reduced to 128. Results show that most of the studies were conducted in countries with socio-economic and technological advancement, mainly South Africa followed by Nigeria and Kenya. The studies were conducted at various scales ranging from field to country level with field scale studies being the most common. Most studies were conducted in relatively small farms typical of most farmlands in SSA. Studies done in relatively large farms are fewer, and such farms would likely belong to a few organisations and individuals with high economic capacity. Many of these studies have been conducted by researchers from outside SSA and a combination of researchers from within and outside SSA. However, based on authorship of the articles, it appears that most of the studies conducted in SSA on precision agriculture have either involved or depended on non-African researchers. It is concluded that there have been significant strides towards use of precision agriculture in SSA. However, with about 21 countries having no research done, there exists greater potential for precision agriculture in the region. Besides, there is need for more research to investigate the low usage of precision agriculture for livestock management.

  相似文献   
66.
Eleven experimental and three commercial white quality protein maize (QPM) hybrids and two regular endosperm controls were planted at Celaya, Guanajuato, Mexico with the aim of comparing grain physical characteristics, protein quality, lime-cooking and tortilla making properties. All genotypes were planted under irrigation using a density of 80,000 plants/ha and fertilized with 250 kg N-60 P-60 K per hectare. When compared with the controls these QPM genotypes had lower test (77.4 vs. 76.5 kg/hL) and 1,000 kernel weights (327 vs. 307 g), softer endosperm texture (2.5 vs. 1.8 where 1 = soft, 2 intermediate and 3 hard endosperm), lower protein (10.0 vs. 8.0%), higher nixtamal water uptake after 30 min lime-cooking (50.0 vs. 53.1% moisture) and lower pericarp removal scores. The lower thousand-kernel weight and softer endosperm texture observed in the QPM genotypes lowered the optimum lime-cooking time as estimated with regression equations. Most QPM genotypes had higher amounts of lysine, tryptophan and albumins/globulins when compared with the controls. QPMs HEC 424973, HEC 774986 and HEC 734286 had the best grain traits for nixtamalization and therefore the best potential for industrial utilization. The commercial use of these QPM hybrids should benefit Mexicans who depend on tortillas as the main staple.  相似文献   
67.
To understand the distribution pattern and divergence of Rhizoctonia solani in a field over a 4-year period, R. solani AG1-IA isolates were collected from diseased tissues of several crops. Pairing tests between isolates to detect hyphal anastomosis and vegetatively compatible population (VCP) groupings were done on 2% water agar and potato dextrose agar. A single VCP of R. solani AG1-IA dominated a large upland crop field at the Institute of Plant Breeding, University of the Philippines at Los Ba?os. The VCP changed more slowly and at a lower frequency as compared to other reports. Received 27 September 1999/ Accepted in revised form 3 February 2000  相似文献   
68.
Summary A reverse of the delaying effect of gibberellic acid (GA3) was observed in in vitro tuberization of potato plantlets when the medium contained 0.8×10−5 M jasmonic acid (JA). The promoting effect of JA seemed to be correlated with the initial absence of growing roots, probably through direct activity of JA in microtuber-producing buds in the stoloniferous shoots. This is based on the fact that JA did not inhibit root growth a posteriori. Endogenous gibberellins (GAs) synthesized by roots did not interfere with the previous activity of JA in tuberization. The absence of a JA promoting-effect on tuberization of previously-rooted plantlets could be related to the capacity of potato roots to synthesize endogenous GAs which might antagonize the JA effect on buds of stoloniferous shoots.  相似文献   
69.
Plant Foods for Human Nutrition - Sorghum is a major cereal crop with various agronomic advantages, contains health-promoting compounds and is gluten-free. There is a growing tendency to use...  相似文献   
70.
动物精子的性别可通过流式精子分选仪和DNA标识进行鉴定,而利用鉴定性别的精子(即性控精子)并借助人工授精技术或其它授精技术产生的后代,在过去5年中估计已多达30000个(其中大多数是母牛)。有关文献资料证明,能有效地区分X精子和Y精子的唯一标记物是精子染色体中的DNA。众所周知,目前世界各地采用的方法是Beltsville精子分选技术,该技术根据X精子群和Y精子群中DNA相对含量上的差异,用荧光染液(Hoechst33342染液)标记精子,随后利用流式细胞仪分选经荧光标记的精子,从而达到分离精子的目的。目前,X精子或Y精子正常的生产速度是每小时1500万个,该项技术已在家畜、实验动物和动物园动物中应用,如果将该技术应用在人上,在预选后代性别比例上可达90%~95%的成功率。因动物品种不同,性控精子在动物体内的授精部位也不相同。常规的人工授精技术、宫内授精技术、输卵管内授精技术、用于胚胎移植的体外受精(in-vitro fertilization,IVF)技术或子宫角深部授精技术均能有效地使动物怀孕,至于利用哪种技术进行性控精子的精则取决于动物品种。尽管所有动物都能获得高纯度的分选精子,但是在实际生产中利用低剂量精子还难以让母猪怀孕。子宫角深部授精技术每次授精0.5~1.00亿个性控精子已能产生可喜的效果:利用特制的输精管,输入常规人工授精所需精子量的五十分之一的性控精子,足以使动物怀孕。性控精子通过常规的授精技术能够被猪成功利用前,还需重新设计输精管,同时输精的次数和每次授精时精子数量也需作进一步的研究。分选精子的低温保存技术已被牛人工授精普遍采用。尽管已能利用冷冻的性别分选精子生产小猪,但是性别分选精子经冷冻和解冻处理后在常规生产中的应用还未达到最理想的效果。本文将讨论猪精子性别分选的最新研究成果及其发展趋势,并重点探讨将性别分选技术应用于养猪生产中必须对其进行必要的技术开发。  相似文献   
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