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A high frequency of sprouting (80.0%) and shoot differentiation was observed in the primary cultures of nodal explants of Morus indica L. cultivar M-5 on MS medium supplemented with 2,4-D (0.3 mg/l). In vitro proliferated shoots were multiplied rapidly by culture of shoot tips on MS medium with BAP (0.5 and 1.0 mg/l) which produced the greatest multiple shoot formation. Multiplication was also achieved by culture of shoot tips on MS medium with BAP (4.0 mg/l) and GA3 (0.05 mg/l) which facilitated the elongation of shoots followed by sprouting of axillary buds of in vitro grown shoots. A high frequency of rooting (86.7%) with development of healthy roots was observed from shoots cultured on medium with 2,4-D (1.0 mg/l). Plants with well developed roots were transferred to soil with a survival frequency of 80%. 相似文献
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Soil cadmium (Cd) causes toxicity and oxidative stress, alters biochemical processes and rootknot formation in rice. Irrigation of exogenous peroxidase (POX) together with its co-substrate H2O2(POXRice + H2O2), is likely to have protective effect upon the biochemical and nodular changes in ricegrown in Cd-rich soil. Exposure to Cd concentration of 1.00 mg/L increased oxidative stress, loss of cellviability, electrolyte leakage and root knot formation, whereas it significantly lowered the chlorophyll leveland rhizobium growth in rice. Irrigation of exogenous POXRice + H2O2 to Cd-stressed rice seedlingsreversed the Cd-induced alterations in rice to levels similar in control (non-stressed) seedlings. Resultsprovided strong evidence of exogenous POXRice + H2O2-mediated reversal and restoration of physiologicaland biochemical processes as well as increased resistance of rice seedlings to root knot formation.Irrigation with POXRice + H2O2 appeared to contribute towards bringing normoxic conditions in the otherwisehypoxic soil environment by enhancing the O2 in pot-experiments due to reduced Cd uptake, enhancedmineral homeostasis of essential elements viz. P, Fe, Mo, Mg and Mn for maintenance of root architecturedamaged by lipid peroxidation and reduction in oxidative stress by reducing Cd-induced reactive oxygenspecies generation. Therefore, the mitigation of Cd-toxicity in rice through this novel approach appeared tobe a promising mode to limit Cd-uptake, modulate protective and tolerance mechanisms for sustainablerice yield in Cd-contaminated rice-croplands and prevent nematode attack in rice, however, more detailedstudies are needed prior to large scale applications. 相似文献
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Pretreatment of radish (Raphanus sativus L.) seedlings with exogenous hormones in the absence of external nitrate, resulted in a system having enough hormone levels to mediate the responses or activate the metabolic pathways which are necessary for nitrate (NO3) uptake and reduction. Effects of pretreatment of radish seedlings with KN, GA, and ABA on the induction of NO3 transport and corresponding NR activity, upon exposure to NO3 were investigated. A low NO3, uptake rate was observed with hormones when compared to DW‐control, while its induction pattern exhibited a sort of biphasic kinetics. It was observed that each hormone treatment affected NO3 uptake and reduction specifically at different ambient concentrations of NO3. On the basis of this, the operation of different constitutive and inducible (CHATS, LATS, HATS, and IHATS) transport systems was resolved for different hormonal treatments. Further, the hypothesis that nitrate uptake has direct role on nitrate reduction was supported by all treatments, except KN where highest NR activity despite lowest NO3 uptake, was recorded. The results with KN points towards enhanced NR mRNA and de novo synthesis of enzyme. 相似文献
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U. Chitra U. Singh P. Venkateswara Rao 《Plant foods for human nutrition (Dordrecht, Netherlands)》1996,49(4):307-316
The objective of this project was to determine the effect of various types of processing on selected nutrition related parameters of commonly consumed Indian pulses and soybean. Germination reduced the phytic acid content of chickpea and pigeonpea seeds by over 60%, and that of mung bean, urd bean, and soybean by about 40%. Fermentation reduced phytic acid contents by 26–39% in all these legumes with the exception of pigeonpea in which it was reduced by more than 50%. Autoclaving and roasting were more effective in reducing phytic acid in chickpea and pigeonpea than in urd bean, mung bean, and soybean. Germination and fermentation greatly increased the in vitro protein digestibility (IVPD). IVPD was only slightly increased by roasting and autoclaving of all legumes. Germination and fermentation also remarkably decreased the total dietary fiber (TDF) in all legumes. Autoclaving and roasting resulted in slight increases in TDF values. All the processing treatments had little effect on calcium, magnesium and iron contents. 相似文献
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New Forests - The East Indian sandalwood (Santalum album L), one among the valued timber species in the global market due to its sweet-scented heartwood, is facing a drastic decline of its natural... 相似文献
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OBJECTIVES: To ascertain the breakfast habits of 10-15-year-old schoolchildren and to assess the quality of this meal as well as its relationship to the food consumption pattern for the full day. DESIGN: Cross-sectional survey. SUBJECTS AND SETTING: Eight hundred and two schoolchildren, boys and girls, aged 10-15 years, belonging to different urban schools located in Secunderabad, Andhra Pradesh, India. METHOD: The breakfast eating patterns of the children were studied and their impact on growth was assessed, using dietary recalls and anthropometric measurements. RESULTS: Only 42.8% of the children ate breakfast regularly. Over half of the children skipped breakfast, ranging from daily to once in two weeks. The energy and protein composition of breakfasts eaten by the children indicated that those who did not skip breakfast met one-quarter to one-third of their total daily energy and protein requirements. Mean nutrient intakes calculated from 24-hour recalls revealed that the children's diets were inadequate compared with the recommended values for energy and protein. The inadequate energy intake was reflected in a high incidence of malnutrition in both boys and girls; 40.3% of the boys and 32.1% of the girls studied were found to be underweight. Protein intake was also inadequate among boys and girls, although a higher percentage of children met their protein requirements. CONCLUSIONS: Over half of the schoolchildren studied skipped breakfast frequently, the main reason being getting up late. Children who consumed breakfast had higher daily intakes of energy and protein than children who skipped breakfast. These data confirm the importance of breakfast to overall dietary quality and adequacy in school-aged children. 相似文献