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551.
Rural habitat as risk factor for hepatitis E virus seroconversion in HIV‐infected patients: A prospective longitudinal study 下载免费PDF全文
A. Martinez‐Peinado A. Camacho L. M. Real M. Frias A. Gordon S. Cantisán J. Torre‐Cisneros J. A. Pineda A. Rivero 《Zoonoses and public health》2017,64(7):e60-e64
Our objective was to determine the incidence and clinical manifestations of acute hepatitis E virus (HEV) in HIV‐infected patients. A prospective longitudinal study including HIV‐infected HEV‐seronegative patients was conducted; HEV seroconversion (to IgG and/or IgM) was the main outcome variable. All patients were tested for HEV antibodies every 3–6 months. For patients who developed HEV seroconversion, a data collection protocol was followed to identify associated clinical manifestations and analytical alterations. A total of 627 patients (89.9%) were followed during a median of 11.96 months (IQR: 8.52–14.52 months) and formed the study population. Forty‐one patients developed detectable anti‐HEV antibodies (7.2 cases per 100 patients/year). Our study found a high incidence of HEV in HIV‐infected patients in southern Spain strongly associated with a rural habitat. 相似文献
552.
Pharmacokinetics and distribution in interstitial and pulmonary epithelial lining fluid of danofloxacin in ruminant and preruminant calves 下载免费PDF全文
D. A. Mzyk R. E. Baynes K. M. Messenger M. Martinez G. W. Smith 《Journal of veterinary pharmacology and therapeutics》2017,40(2):179-191
The objective of this study was to compare active drug concentrations in the plasma vs. different effector compartments including interstitial fluid (ISF) and pulmonary epithelial lining fluid (PELF) of healthy preruminating (3‐week‐old) and ruminating (6‐month‐old) calves. Eight calves in each age group were given a single subcutaneous (s.c.) dose (8 mg/kg) of danofloxacin. Plasma, ISF, and bronchoalveolar lavage (BAL) fluid were collected over 96 h and analyzed by high‐pressure liquid chromatography. PELF concentrations were calculated by a urea dilution assay of the BAL fluids. Plasma protein binding was measured using a microcentrifugation system. For most preruminant and ruminant calves, the concentration–time profile of the central compartment was best described by a two‐compartment open body model. For some calves, a third compartment was also observed. The time to maximum concentration in the plasma was longer in preruminating calves (3.1 h) vs. ruminating calves (1.4 h). Clearance (CL/F) was 385.15 and 535.11 mL/h/kg in preruminant and ruminant calves, respectively. Ruminant calves maintained higher ISF/plasma concentration ratios throughout the study period compared to that observed in preruminant calves. Potential reasons for age‐related differences in plasma concentration–time profiles and partitioning of the drug to lungs and ISF as a function of age are explored. 相似文献
553.
Briana L. Gross Marshall J. Wedger Marlyn Martinez Gayle M. Volk Cindy Hale 《Genetic Resources and Crop Evolution》2018,65(5):1317-1327
Apple (Malus?×?domestica Borkh.) trees, either abandoned or cared for, are common on the North American landscape. These trees can live for decades, and therefore represent a record of large- and small-scale agricultural practices through time. Here, we assessed the genetic diversity and identity of 330 unknown apple trees in northern Minnesota with 9 simple sequence repeat (SSR) markers. The unknown (not identified by cultivar name) trees were compared to >?1000 named cultivars in the U.S. Department of Agriculture Plant Genetic Resources Unit Malus collection and also to each other to identify repeated genotypes. Overall, the 330 unknown trees had high diversity (average He?=?0.75), and consisted of 264 unique genotypes. A total of 76 of the unknown trees were matched to 20 different named cultivars, and these cultivars were mainly derived from either the local breeding program at the University of Minnesota, or were Russian cultivars imported for horticulture in the northern US. This study demonstrates the importance of local breeding programs, and also the challenges associated with identifying clones in a genetically diverse crop like apple. 相似文献
554.
Núbia Rosa da Silva Maaike De Ridder Jan M. Baetens Jan Van den Bulcke Mélissa Rousseau Odemir Martinez Bruno Hans Beeckman Joris Van Acker Bernard De Baets 《Annals of Forest Science》2017,74(2):30