High‐throughput identification and quantification of Vagococcus salmoninarum by SYBR Green I‐based real‐time PCR combined with melting curve analysis     

Yolanda Torres‐Corral  Clara Fernndez‐lvarez  Ysabel Santos 《Journal of fish diseases》2019,42(10):1359-1368

This work describes a primer pair and a high‐throughput SYBR Green I‐based real‐time PCR protocol combined with melting curve analysis for identification and quantification of Vagococcus salmoninarum in bacterial cultures and infected fish tissues. The 16S rRNA gene was selected for the design of the primer pair (SalF and SalR). The sensitivity and specificity of this primer pair were compared with other previously designed for conventional PCR. Although both primer pairs showed 100% specificity using pure bacterial cultures or DNA extracted from bacteria or fish tissues, the primer pairs designed in this study showed the highest sensitivity with a detection limit of 0.034 × 10⁰ amplicon copies per assay (equivalent to 2 × 10^?11 ng/µl, C_q value of 30.49 ± 1.71). The developed qPCR protocol allowed the detection of V. salmoninarum in non‐lethal and lethal fish samples with detection levels of 0.17 × 10⁰ gene copies in tissues artificially infected and 0.02 × 10⁰ in tissues of fish experimentally infected with V. salmoninarum. The high sensitivity of the developed method suggests that it could be considered as a useful tool for diagnosis of vagococcosis and the detection of V. salmoninarum in asymptomatic or carrier fish.  相似文献   

Specificity dependence between serological tests for diagnosing bovine brucellosis in Brucella-free farms showing false positive serological reactions due to Yersinia enterocolitica O:9     

Mainar-Jaime RC  Muñoz PM  de Miguel MJ  Grilló MJ  Marín CM  Moriyón I  Blasco JM 《The Canadian veterinary journal. La revue veterinaire canadienne》2005,46(10):913-916

When brucellosis false positive serological reactions happen in cattle, the serial use of pairs of specificity-correlated serological tests (rose bengal, complement fixation, competitive ELISA) results in specificities lower than expected. In this situation, highly specific tests, such as the indirect ELISA used alone, may be more adequate than serial testing.  相似文献   

Alfaxalone for total intravenous anaesthesia in bitches undergoing elective caesarean section and its effects on puppies: a randomized clinical trial          下载免费PDF全文

Clara Conde Ruiz  Andrea P Del Carro  Emilie Rosset  Emilie Guyot  Laura Maroiller  Samuel Buff  Karine Portier 《Veterinary anaesthesia and analgesia》2016,43(3):281-290

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Designing Nutritious and Sustainable Biscuits Using Upcycled Fibre-Rich Ingredients Obtained by Hot Air - Microwave Drying of Orange by-Products     

Talens  Clara  Rios  Yolanda  Alvarez-Sabatel  Saioa  Ibargüen  Mónica  Rodríguez  Raquel 《Plant foods for human nutrition (Dordrecht, Netherlands)》2022,77(2):271-278

Plant Foods for Human Nutrition - Circular use of resources implies developing mild processes to transform food by-products into value-added products, without using organic solvents or extensive...  相似文献   

Arthroscopic Biceps Ulnar Release Procedure (BURP): Technique Description and In Vitro Assessment of the Association of Visual Control and Surgeon Experience to Regional Damage and Tenotomy Completeness     

David M. Wilson DVM  Clara S.S. Goh BVSc  MS  Diplomate ACVS  Ross H. Palmer DVM  MS  Diplomate ACVS 《Veterinary surgery : VS》2014,43(6):734-740

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The competitive cost of antibiotic resistance in Mycobacterium tuberculosis     

Gagneux S  Long CD  Small PM  Van T  Schoolnik GK  Bohannan BJ 《Science (New York, N.Y.)》2006,312(5782):1944-1946

Mathematical models predict that the future of the multidrug-resistant tuberculosis epidemic will depend on the fitness cost of drug resistance. We show that in laboratory-derived mutants of Mycobacterium tuberculosis, rifampin resistance is universally associated with a competitive fitness cost and that this cost is determined by the specific resistance mutation and strain genetic background. In contrast, we demonstrate that prolonged patient treatment can result in multidrug-resistant strains with no fitness defect and that strains with low- or no-cost resistance mutations are also the most frequent among clinical isolates.  相似文献   

A circular dichroism and fluorescence spectrometric assessment of effects of selected chemical denaturants on soybean (Glycine max L.) storage proteins glycinin (11S) and beta-conglycinin (7S)     

Clara Sze KW  Kshirsagar HH  Venkatachalam M  Sathe SK 《Journal of agricultural and food chemistry》2007,55(21):8745-8753

Soybean glycinin (11S) and beta-conglycinin (7S) were subjected to select chemical treatments at various concentrations and resulting changes in protein structures were investigated by circular dichroism (CD) and fluorescence spectrometry. Fluorescence quenching results indicated that urea >/=3 M caused significant unfolding of 11S, but not that of 7S. GuHCl was more effective than urea in denaturation of 11S. A two-step transition in 11S structure was observed with a possible existence of a folding intermediate at 2.5 M GuHCl. Sodium dodecyl sulfate (SDS) measurably altered secondary and tertiary structures of 11S and 7S below SDS critical micellar concentration (CMC), possibly due to formation of mixed peptide-SDS micelles. SDS treatment increased alpha-helical and unordered structures of both proteins at the expense of beta-sheet structure. NaCl and CaCl 2 caused a significant decrease in fluorescence intensity without shifting emission lambda max. Exposure of 7S and 11S to NaSCN respectively at >/=0.3 and >/=0.6 M NaSCN caused a significant increase in fluorescence intensity measured at the corresponding lambda max of the protein. beta-Mercaptoethanol (beta-ME), N-ethylmaleimide (NEM), and phytic acid caused variable red shifts, 2.5-4 nm, in the emission lambda max.  相似文献   

Avian tuberculosis of zoonotic importance at a zoo on the Bogotá Andean plateau (Sabana), Colombia     

Angela del Pilar Silva  Clara Inés Leon  Martha Inírida Guerrero  Rafael Neira  Leonardo Arias  German Rodriguez 《The Canadian veterinary journal. La revue veterinaire canadienne》2009,50(8):841-845

Given that exposure to captive wild animals at circuses or zoos can be a source of zoonotic infection, a case and control study was carried out with a collection of exotic fowl at a zoo in Bogotá, Colombia. The presence of Mycobacterium avium-II was directly related to the death of birds kept in the original enclosure, and of 50% of a group of sentinel birds. Failure to detect the organism in a control group of birds outside the enclosure indicated that the infection was limited to the original enclosed area. We demonstrated that M. gordonae-IV was disseminated in all organs from 1 bird with macroscopic granulomatous lesion, a finding which has not been reported previously. We emphasize the importance of establishing handling norms to reduce the risk of zoonotic transmission.  相似文献   

Biology and molecular characterization of necroviruses affecting <Emphasis Type="Italic">Olea europaea</Emphasis> L.: a review     

Maria do Rosário Félix  Carla M. Reis Varanda  Maria Ivone E. Clara 《European journal of plant pathology / European Foundation for Plant Pathology》2012,133(1):247-259

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Cryptogein and capsicein promote defence responses in Quercus suber against Phytophthora cinnamomi infection     

Clara Medeira  Virgínia Quartin  Isabel Maia  Inês Diniz  Ma Céu Matos  José Nobre Semedo  Paula Scotti-Campos  José C. Ramalho  Isabel P. Pais  Paula Ramos  Elvira Melo  António E. Leit?o  Alfredo Cravador 《European journal of plant pathology / European Foundation for Plant Pathology》2012,134(1):145-159

The decline of cork oak (Quercus suber) stands in Iberian Peninsula is associated with infection by Phytophthora cinnamomi. Most Phytophthora species secrete elicitins, which can enhance defence reactions against some pathogens. Here cytological and physiological effects of the elicitins cryptogein and capsicein on cork oak root infection by P. cinnamomi were evaluated. The progression of the pathogen in root tissue and its effects on total fatty acid (TFA) composition of roots and leaves were analysed in seedlings. Net photosynthesis (P _n ), stomatal conductance (g _s ), chlorophyll a fluorescence measurements (quantum yield of linear electron transport ? _e , photochemical quenching q _P, non-photochemical quenching NPQ) and carotenoid determinations were carried out in well established (4?months) plants. In elicitin-treated roots, 2?days after inoculation, the pathogen which presented loss of viability and membrane degradation was mainly restricted to the intercellular spaces of the cortical parenchyma, and did not reach the vascular cylinder. Electron dense materials accumulated in the intercellular spaces of the cortex next to disorganized hyphae, suggested to be related with defence reactions. Cryptogein (or its interaction with P. cinnamomi) induced enhanced lipid synthesis in leaves, which may contribute to preserve membrane stability. P. cinnamomi decreased P _n , g _s , ? _e , and q _P, whereas elicitin-treated plants displayed values similar to controls. Overall, the results indicated a resistance response of cork oak against this oomycete, induced by the elicitins.  相似文献