Cloning and expression in Escherichia coli of Mycoplasma gallisepticum antigens recognized by sera from infected chickens     

D C Krause  S H Kleven  K K Lee 《Avian diseases》1990,34(2):466-472

A clone bank of Mycoplasma gallisepticum (MG) strain A5969 DNA was prepared in the expression vector phage lambda gt11. Approximately 75% of the resulting phages were recombinants, based upon the insertional inactivation of the lacZ gene of the vector. Clones were screened immunologically with serum prepared from specific-pathogen-free white leghorn chickens that had been infected with aerosolized MG. Approximately 250 clones, or less than 1% of the recombinant phage, reacted positively to various degrees with the test serum and failed to react with serum from uninfected specific-pathogen-free control chickens. A single clone was chosen at random for comparison with a vector control by western immunoblot, revealing a polypeptide of 140,000 molecular weight in the clone profile but not the control profile that reacted with immune serum. Clones expressing MG antigens recognized during infection may provide an improved means for antigen preparation for serologic diagnosis of mycoplasmosis.  相似文献   

Viewpoints on synergising ASEAN academic visibilities through research collaboration and the establishment of an ASEAN Citation Index Database1     

Narongrit Sombatsompop  Nongyao Premkamolnetr  Teerasak Markpin  Santi Ittiritmeechai  Chatree Wongkaew  Wutthisit Yochai  Preeyanuch Ratchatahirun  Lee Inn Beng 《Asia Pacific viewpoint》2011,52(2):207-218

This opinion article expresses two key viewpoints regarding the options for Association of South‐East Asian Nations (ASEAN) universities in pursuit of fulfilling the demand for greater research visibility and academic reputation. The first viewpoint outlines the importance of promoting research collaboration among ASEAN researchers and their international peers in increasing the visibility and impact of ASEAN research. The second viewpoint examines the ASEAN research characteristics, the challenges and existing best practices of its journal publishing landscape, the potential role of non‐ASEAN international journals in improving the profile of ASEAN research and explores the needs and benefits of establishing an ASEAN Integrated Journal Publishing Network (IJPN). It is through these combined approaches, we believe, that effective policy change, the standardisation of publishing structure, procedures, and systems to drive journal development, improvement of journal quality, and establishment of a consistent framework for measuring researcher performance will result. This article also includes a proposal for the IJPN structure and implementation plan, and highlights the key benefits of taking a collaborative approach to achieving this common goal.  相似文献   

Movement of nutrient and non-nutrient elements in the liquid phase in sheep rumen     

K N Joblin  J Lee 《Journal of animal science》1990,68(7):2067-2074

Changes in the concentrations of B, Ca, Fe, K, Mg, Mn, Na, P, S, Sr and Zn in the liquid phase in sheep rumen were determined to obtain information on their rate of disappearance from the soluble pool after feeding. Sheep were fed alfalfa (Medicago sativa) hay hourly. The concentration of infused Cr(III)-EDTA into the rumen (over a period of 4 d) was used to establish steady-state conditions, after which feeding was stopped and the rapidly changing elemental concentrations in 30,000 x g ruminal supernatant fluid were determined by plasma emission spectrometry. The concentrations of all elements, apart from Fe, Na and P, decreased in a first-order manner over 5 h after feeding. For one sheep, these concentrations (microM) were: B, 199 to 126; Cr, 184 to 111; Ca, 4,150 to 1,130; K, 51,000 to 28,800; Mn, 11 to 4.2; Mg, 4,190 to 1,810 and Zn, 14 to 7.8. For S, first-order decay was observed over only the 0- to 2-h period (2230 microM to 865 microM). Phosphorus increased from 10.4 to 13.1 mM over the 5-h period, whereas Na remained constant (85 mM); this was attributed to saliva input. Mean half-lives for B (8.8 h), K (7.5 h) and Zn (5.3 h) were similar to those of Cr (6.3 +/- .3 h), the water flow marker. Mean half-lives of Ca (2.6 h), Mg (4.0 h), Mn (3.3 h), Sr (3.3 h) and S (1.8 h) were shorter (P less than .01) than that of the Cr marker.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Alteration in intestinal morphologic features associated with extensive large-colon resection in horses     

A L Bertone  G L Cockerell  R E Lee  T S Stashak 《American journal of veterinary research》1990,51(9):1471-1475

Light microscopy, morphometry, and scanning electron microscopy were used to examine the mucosal morphologic features of 7 intestinal specimens (3 from the small intestine; 4 from the large intestine) from each of 8 horses 1 year after sham operation (group 1; n = 3) or extensive large-colon resection (group 2; n = 5). Qualitative light microscopic examination did not reveal differences between groups, but morphometry revealed significantly (P less than 0.05) greater intercrypt area and distance in horses with colon resection and this was most pronounced in the cecum and remaining right ventral and dorsal colon. Crypt area and depth were similar for horses with colon resection and sham operation (P greater than 0.05). Qualitative evaluation of the scanning electron micrographs revealed more prominent crypt orifices in the large intestine of horses with colon resection. The larger intercrypt distance in the colon of horses with resection was not an obvious feature of the qualitative evaluation of the surface with scanning electron microscopy. Small intestinal morphologic features were variable and significant differences were not detected between horses with sham operation and colon resection. Horses adapted to extensive large-colon resection within 1 year by increasing the absorptive (intercrypt) surface area of the remaining large intestine.  相似文献   

College News     

R. Lee Pyle VMD 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1990,4(5):275-275

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Apparent Digestibility Coefficients for Some Carbohydrates in Diets for Hybrid Striped Bass Morone saxatilis × M. chrysops     

Margie Lee  Gallagher 《Journal of the World Aquaculture Society》1997,28(4):429-431

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Pharmacokinetics of single doses of digoxin administered intravenously to ducks, roosters, and turkeys     

W M Pedersoli  W R Ravis  H S Lee  L M Krista  J S Spano 《American journal of veterinary research》1990,51(11):1751-1755

A single dose of digoxin was injected, IV, into 5 mature male turkeys (0.066 mg/kg of body weight), 8 male ducks (0.066 mg/kg), and 6 roosters (0.33 mg/kg). Twenty-three serial venous blood samples were collected before (baseline) and after the administration of digoxin to turkeys, ducks, and roosters. Plasma concentrations of digoxin were determined in duplicate by a radioimmunoassay that was validated for avian species. The plasma concentrations were best fitted by a 3 (turkeys, ducks)- and 2 (roosters)-compartment open model, with first-order elimination from the central compartment. Significant (P less than 0.05) kinetic differences were determined among species. Mean half-life (t1/2) for ducks, roosters, and turkeys were 8.30 +/- 2.70 (mean +/- SD), 6.67 +/- 3.50, and 23.7 +/- 4.8 hours, respectively. The volume of distribution at steady state (Vss) was 14.7 +/- 2.9, 3.13 +/- 0.49, and 2.27 +/- 0.36 L/kg, and total body clearance (CL) of drug was 1.54 +/- 0.43, 0.461 +/- 0.187, and 0.136 +/- 0.022 L/h/kg for ducks, roosters, and turkeys, respectively. The mean residence time was 10.3 +/- 3.9, 8.37 +/- 4.97, and 16.8 +/- 2.2 hours, respectively. Volume of distribution at steady state and CL in ducks were several fold higher than that in turkeys. The terminal half-life of digoxin determined for ducks and roosters in this study was considerably shorter than those previously reported for several mammalian species.  相似文献   

Determining the optimum digestible isoleucine to lysine ratio for Ross 708 x Ross YP male broilers from 0 to 18 d of age     

A.T. Brown  J. Lee  R. Adhikari  K. Haydon  K.G.S. Wamsley 《The Journal of Applied Poultry Research》2022,31(1):100217

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Failure of excess leucine to impact live performance and carcass traits in male and female Cobb MV × 500 broilers during a 15- to 32-day grower period     

C.W. Maynard  G.J. Mullenix  C.J. Maynard  J.T. Lee  S.K. Rao  L.D. Butler  J.Z. Hiltz  S.K. Orlowski  M.T. Kidd 《The Journal of Applied Poultry Research》2022,31(2):100242

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Titration of dietary isoleucine and evaluation of branched-chain amino acid levels in female Cobb 500 broilers during a 22- to 42-day finisher period     

C.W. Maynard  G.J. Mullenix  C.J. Maynard  S.C. Wells-Crafton  J.T. Lee  S.K. Rao  L.D. Butler  S.K. Orlowski  M.T. Kidd 《The Journal of Applied Poultry Research》2022,31(2):100245

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