Extrinsic cardiac nerve segments in the domestic dog (Canis familiaris- Linnaeus, 1758). Comparative study in young and adult dogs     

Brugnaro M  De Souza RR  Ribeiro AA 《Anatomia, histologia, embryologia》2003,32(4):228-232

In this paper, important connections between the two main contingents of the autonomic nervous system, intrinsic and extrinsic visceral plexus were analysed. Concerning heart innervation, the territories of extrinsic innervation are very important in the treatment of congenital or acquired cardiopathy, thoracic neoplasia and aortic arch persistence, among others. This research compared young and adult extrinsic cardiac innervation and described the surgical anatomic nerve segments. Animals were perfused with a 10% formaldehyde solution in PBS (0.1 m) (pH 7.4) and submitted to macro- and meso-scopic dissection immersed in 60% acetic acid alcoholic solution and 20% hydrogen peroxide aqueous solution. The nerve segments were assigned as: right vagus nerve segment, left vagus nerve segment, right middle cervical ganglion segment, left middle cervical ganglion segment, right caudal laryngeal nerve segment, left caudal laryngeal nerve segment, right phrenic nerve segment and left phrenic nerve segment.  相似文献   

Effects of nonesterified fatty acids and beta-hydroxybutyrate on functions of mononuclear cells obtained from ewes     

Lacetera N  Franci O  Scalia D  Bernabucci U  Ronchi B  Nardone A 《American journal of veterinary research》2002,63(3):414-418

OBJECTIVE: To assess the effects of nonesterified fatty acids (NEFA) and beta-hydroxybutyrate (BHBA) on functions of mononuclear cells obtained from ewes. ANIMALS: 6 Sardinian ewes. PROCEDURE: Mononuclear cells were cultured with concentrations of NEFA (0, 15.6, 31.2, 62.5, 125, 250, 500, 1,000, or 2,000 micromol/L) and BHBA (0, 0.45, 0.9, 1.8, or 3.6 mmol/L). Concentrations of NEFA and BHBA were intended to mimic those of ketotic or healthy ewes, and NEFA and BHBA were tested alone and in combination. Synthesis of DNA was stimulated by use of concanavalin A (Con A) or pokeweed-mitogen (PWM). Secretion of IgM was stimulated by use of PWM. RESULTS: Synthesis of DNA stimulated by Con A and PWM was significantly inhibited by high concentrations of NEFA (> or = 250 micromol/L) or by a combination of high concentrations of NEFA (> or = 250 micromol/L) and all concentrations of BHBA (> or = 0.45 mmol/L). In contrast, DNA synthesis was not inhibited by low concentrations of NEFA (< or = 125 micromol/L) or by a combination of low concentrations of NEFA (< or = 125 micromol/L) and the lowest concentration of BHBA (0.45 mmol/L). Secretion of IgM was significantly inhibited by all concentrations of NEFA and by all combinations of NEFA and BHBA concentrations. When used alone, none of the concentrations of BHBA inhibited DNA synthesis or IgM secretion. CONCLUSIONS AND CLINICAL RELEVANCE: Reduced immunoresponsiveness during ketosis is likely to be associated with an increase in plasma concentration of NEFA and not with an increase in plasma concentration of BH BA.  相似文献   

Effects on functions of ovine blood mononuclear cells for each of several fatty acids at concentrations found in plasma of healthy and ketotic ewes     

Lacetera N  Franci O  Scalia D  Bernabucci U  Ronchi B  Nardone A 《American journal of veterinary research》2002,63(7):958-962

OBJECTIVE: To assess effects on functions of peripheral blood mononuclear cells (PBMC) obtained from ewes for each of several fatty acids represented in ovine plasma at concentrations mimicking those of ketotic or healthy ewes. SAMPLE POPULATION: Blood samples obtained from 6 Sardinian ewes. PROCEDURE: The PBMC were cultured in media that contained oleic (OA), palmitic (PA), stearic (SA), linoleic (LA), or palmitoleic (POA) acid at concentrations similar to those of ketotic or healthy ewes. Synthesis of DNA was stimulated by use of concanavalin A or pokeweed mitogen (PWM). Secretion of IgM was stimulated by use of PWM. RESULTS: High concentrations (900, 450, and 225 micromol/L) of OA significantly inhibited DNA synthesis and IgM secretion of PBMC. Conversely, low concentrations (56 or 28 micromol/L) of OA significantly enhanced DNA synthesis of PBMC. High concentrations of PA (600, 300, 150, 75, 375, or 18.7 micromol/L) and SA (300, 150, or 75 micromol/L) significantly inhibited DNA synthesis of PBMC. High concentrations of PA (600, 300, 150, 75, 375, or 18.7 micromol/L) and SA (300, 150, 75, or 38 micromol/L) also significantly inhibited IgM secretion of PBMC. None of the concentrations of LA and POA affected PBMC functions. CONCLUSION AND CLINICAL RELEVANCE: Impaired immunoresponsiveness of ketotic ewes is likely associated with an increase of plasma concentrations of OA, PA, or SA and not with that of LA or POA. At physiologic concentrations, single fatty acids are likely to participate in modulation of immunoresponsiveness by exerting suppressive or stimulatory effects on immune cells.  相似文献   

Diaphragmatic paralysis due to phrenic nerve degeneration in a llama     

Bedenice D  Mazan MR  Kuehn H  Hoffman AM 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2002,16(5):603-606

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Production and characterization of a panel of monoclonal antibodies for the identification of antigens of Babesia bovis     

Figueiredo JF  Martins MS  Ribeiro MF  Passos LM 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2000,47(2):121-126

A panel of monoclonal antibodies was produced and characterized by an indirect fluorescent antibody test (IFAT), an enzyme-linked immunosorbent assay (ELISA) and Western blotting with the aim of identifying antigens of Babesia bovis. After fusion, the resultant hybrids were selected by the IFAT, cloned, maintained in culture in vitro, and cryopreserved in liquid nitrogen. Ten clones producing monoclonal antibodies were found to react against the entire merozoites, three reacted on the surface of the merozoites, and one clone reacted against the polar region of the merozoites. All monoclonal antibodies reacted in ELISA, with the optical density varying from 0.368 to 0.502 (cut off = 0.022). The bands recognized by the monoclonal antibodies in Western blotting had molecular weights ranging from 162 to 19 kDa. Four clones recognized a single band of 73 kDa, and another four did not react in Western blotting.  相似文献   

Potato deforming mosaic disease is caused by an isolate of Tomato yellow vein streak virus     

S. G. Ribeiro  A. K. Inoue-Nagata  J. Daniels  A. C. de Ávila 《Plant pathology》2006,55(4):569-569

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Uncoupling and inhibition properties of 3,4-seco-friedelan-3-oic acid isolated from Maytenus imbricata     

Silvia Ribeiro de Souza e Silva  Luiz Claúdio de Almeida Barbosa  Beatriz King-Diaz  Blas Lotina-Hennsen 《Pesticide biochemistry and physiology》2007,87(2):109-114

3,4-Seco-friedelan-3-oic acid was isolated from Maytenus imbricata (Celastraceae). At low concentrations it inhibited non-cyclic electron transport and ATP synthesis in spinach chloroplasts, i.e., it behaved as a Hill reaction inhibitor, and at high concentrations it acts as an uncoupler by enhancing uncoupled electron transport and Mg²⁺-ATPase activity. 3,4-Seco-friedelan-3-oic acid did not inhibit PSII electron transport from DPC to DCPIP_ox and photosystem I activity, but it enhanced from TMQH₂ to MV, corroborating its action as uncoupler. It inhibits electron flow through PSII from water to sodium silicomolybdate. The whole results indicate that the 3,4-seco-friedelan-3-oic acid target is at the OEC complex enzyme, the donor side of PSII. The fluorescence decay data shows the formation of the K-band, which match this result, acting as inhibitor at the donor side of PSII and it as an uncoupler.  相似文献   

Leaf necrosis and twig dieback of sweet persimmon (Dyospiros kaki L.) caused by Pseudomonas syringae pv. syringae in Italy     

M. Scortichini  D. D'Ascenzo  Daniela Di Silvestro 《European journal of plant pathology / European Foundation for Plant Pathology》1998,104(6):625-627

In spring 1996, extensive leaf necrosis and twig dieback were observed on young sweet persimmon (Dyospiros kaki L.) trees, cultivars O'Gosho, Hachija, Mercatelli and Kaki-tipo planted in the Abruzzo region (central Italy). Many trees were killed. When the dieback reached the trunk, in many cases, new vegetation was noticed above the graft point. The cultivar Jiro-C was not affected by the disease. During 1997, no symptoms were observed on any plant. The orchard was planted in a clay soil with a very low content of organic matter. Biochemical, nutritional and pathogenicity tests indicated Pseudomonas syringae pv. syringae van Hall as the causal agent of the disease. This is the first report of this bacterium as a pathogen of sweet persimmon in Europe.  相似文献   

Variability in feeding ecology of a South American cichlid: a reason for successful invasion in mediterranean‐type rivers?     

F. Ribeiro  R. L. Orjuela  M. F. Magalhães  M. J. Collares-Pereira 《Ecology of Freshwater Fish》2007,16(4):559-569

Abstract –  Ecological attributes behind the success of the South American cichlid Australoheros facetus in mediterranean-type rivers remain unclear. This study addresses this issue, by analysing its abundance and feeding patterns in the lower Guadiana drainage (Portugal), during the dry season of 2003. Despite slight spatial variations in abundance and individual size, A. facetus seemed well established in the Guadiana, Vascão and Ardila rivers. No seasonal or size-related changes in feeding intensity were found, but the diet changed across rivers, over time and throughout ontogeny. Feeding seemed highly generalist, with fish apparently reacting to both spatial and temporal changes in food supply and tending to use the most abundant and easily accessible food items. Overall prey breadth was broad relative to that of other native and non-native species. It is suggested that a generalist feeding strategy may play a significant role in the establishment of non-native fish in mediterranean-type rivers.  相似文献   

The isolation and serology of Brucella melitensis in a flock of goats in central RSA     

L M Ribeiro  S Herr  F Chaparro  F H van der Vyver 《The Onderstepoort journal of veterinary research》1990,57(2):143-144

Brucella melitensis biotype 1 was isolated in pure culture from the lungs, liver, spleen, kidney, stomach contents, abomasum and brain of an aborted caprine (Boer goat) foetus in the district of Cullinan near Pretoria. The 18 does and 1 ram in the flock of Boer goates were examined serologically by means of the complement fixation (CF) test, using Brucella abortus antigen. Six weeks later they were examined again, using B. abortus as well as B. melitensis biotype 1 antigens. No significant differences were found between the 2 CF tests using B. abortus antigen, or between the results obtained by using the B. abortus and B. melitensis antigens. Twelve goats, showing CF antibody titres, were slaughtered and examined bacteriologically. No relationship was found between the serological and bacteriological results.  相似文献