Progress in the utilization of Cajanus platycarpus (Benth.) Maesen in pigeonpea improvement     

Nalini Mallikarjuna  Senthilvel Senapathy  Deepak R. Jadhav  Kulbhushan Saxena  Hari C. Sharma  Hari D. Upadhyaya  Abishek Rathore  Rajeev Varshney 《Plant Breeding》2011,130(5):507-514

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Pyramiding of two bacterial blight resistance and a semidwarfing gene in Type 3 Basmati using marker-assisted selection     

Deepak Rajpurohit  Rahul Kumar  Mankesh Kumar  Priyanka Paul  Anjali Awasthi  P. Osman Basha  Anju Puri  Tripta Jhang  Kuldeep Singh  Harcharan Singh Dhaliwal 《Euphytica》2011,178(1):111-126

A traditional Type 3 Basmati rice cultivar grown in India is tall and lodges even under low nitrogen fertilizer dose. In addition to lodging, it is highly susceptible to several diseases and pests including bacterial blight (BB). BB resistance genes (Xa21 and xa13) and a semidwarfing gene (sd-1) were pyramided in Type 3 Basmati from a rice cultivar PR106-P2 using marker-assisted selection (MAS). Foreground selection for BB resistance genes, Xa21 and xa13 and reduced plant height gene, sd-1 was carried on the basis of linked molecular markers pTA248, RG136 and ‘h’, respectively. The BC₂F₃ progenies with both the BB resistance genes were highly resistant with lower lesion length than either of the genes individually. Background profiling of the selected 16 BC₂F₃ progenies was done using 95 anchored SSR and 12 ISSR markers. Among the selected 16 BC₂F₃ progenies, 38-5-2 and 38-5-36 closely clustered along with the recipient parent Type 3 Basmati showing above 85% genetic similarity with the same. Further selection was continued till F₅ generation for higher recovery for Type 3 Basmati characteristics. The desirable alleles of intermediate amylose content (wx) and aroma (fgr) loci of Type 3 Basmati were also tracked using the linked SSR markers. The BC₂F₅ pyramid lines T3-4, T3-5, T3-6 and T3-7 homozygous for the three target genes Xa21, xa13 and sd-1 from the donor parent with wx and fgr alleles of Type 3 Basmati had excellent cooking quality and strong aroma.  相似文献   

Morphological features of an open flower mutant plant and characterization of their progenies in lentil (Lens culinaris Medik.)     

Meenakshi Dheer  S. S. Punia  Baldev Ram  N. K. Jain  N. R. Koli  V. Bhatnagar  O. P. Khedar 《Genetic Resources and Crop Evolution》2014,61(5):879-886

The lentil (Lens culinaris Medik.) is a self-pollinated seed legume with cleistogamous flowers. A spontaneous open flower semi leaf-less mutant, was observed from segregating generation (F₄) of a cross IPL 313 × RKL 1001 in which reproductive organs were not enclosed by the keel petals and thus remained exposed. Leaves on this mutant plant was very less and random with 1–3 pairs of leaf lets whereas in normal plant leaves are present at every reproductive node with 5–7 pairs of leaf lets. A very large number of open flowers (>90 %) remained sterile in mutant plant and their progenies, though its pollen fertility was as high as the standard cultivars. The progenies of the mutant open flower plant were segregates in three morphological different plant types as (1) open flower leaf-less plants, (2) open flower semi leaf-less plants and (3) normal plants having cleistogamous (closed) flowers and normal bearing leaves. The open flower leafless plant were low yielder whereas open flower semi leafless progenies have good yield potential and are important source of rare novel mutant alleles for important economic traits like multiple peduncles per reproductive node, more number of flowers/pods per peduncle and long reproductive phase. The open flower trait offers opportunity for exploring hybrid technology in the lentil and mutant alleles can play a crucial role in understanding the genetics of the target trait and for improvement of lentils.  相似文献   

Are marketing intermediaries exploiting mountain farmers in Nepal? A study based on market price,marketing margin and income distribution analyses     

Deepak M. Pokhrel  Gopal B. Thapa 《Agricultural Systems》2007

Against the backdrop of viewing marketing intermediaries in developing countries as parasites, this study examined the validity of such a view, especially in the context of mandarin (a species of orange) marketing in a mountain district of Nepal. Necessary information was collected from all major stakeholders such as farmers, collectors and commission agents, and the relative position of farmers in terms of their gains was analyzed by employing three criteria: price of mandarin, marketing margin and income distribution. Income distribution among marketing functionaries “with” and “without” transaction cost has been analyzed. Similarly, marketing margin and farmers’ share of gross income are also analyzed ‘with’ and ‘without’ the cost of malicious practices by marketing intermediaries. Findings of the study revealed that farmers in the study area are receiving a fair share of the benefit accruing from the marketing of mandarin. However, taking advantage of their weak bargaining power and poor economic condition, marketing intermediaries are harassing and cheating them in different ways. Policy recommendations are made to institutionalize and strengthen the group-marketing system to address such inherent problems.  相似文献   

Spontaneous chondrosarcoma in Wistar rat: a case report     

Seema Balani  Mohmad Sadik Mulla  Vijayakumar Subramanian  Jomy Jose  Upendra Bhatnagar 《Journal of toxicologic pathology》2021,34(1):119

In rats, chondrosarcomas have been reported to occur both spontaneously and secondary to chemical induction. In a rare case, a spontaneous chondrosarcoma was identified in the deformed femur of a young male Wistar rat. After gross examination of the femur and knee joint, tissue was collected and preserved. The formalin-fixed tissue was decalcified, embedded in paraffin, sectioned, and stained with hematoxylin and eosin. Microscopic examinations revealed a large, highly proliferative, noncapsulated growth of chondrocytic or chondroblastic origin in the femoral bone, with proliferating chondrocytes invading the bone and surrounding tissues in an infiltrative growth pattern. Based on its histomorphological features, the lesion was diagnosed as a malignant cartilaginous neoplasm of spontaneous origin.  相似文献   

Residues of organochlorine insecticides in two lakes of Jaipur,India     

Surendar Kumar  Rup Lal  Pradeep Bhatnagar 《Water, air, and soil pollution》1988,42(1-2):57-65

The concentration of organochlorine insecticides/metabolites, namely, lindane, heptachlor, aldrin, p,p′-DDL, p,p′-DDD, and p,p′-DDT were determined in water samples taken from two lakes, Jalmahal and Mahalon, from June 1985 to July 1986. These lakes are contaminated with moderate to high level of residues of different insecticides. In Mahalon lake the residues were highest (6.6 μg L^?1) during October 1985 whereas in Jalmahal lake they were highest (9.6 μg L^?1) in September 1985. In both lakes, residues of DDT exceeded the recommended limit for water quality criteria. Lindane was found in most of the samples but in lower quantities. Aldrin was the second most common residue in these lakes.  相似文献   

Effect of isocyanide on the ultrastructure of mouse pineal     

N Chang  K P Bhatnagar 《Anatomia, histologia, embryologia》1984,13(2):181-188

To investigate the possible influence of a strong malodorant on the pineal gland, the effects of tertiary n-butyl isocyanide on the ultrastructure of pinealocytes were studied. Adult male mice were adapted to a 23 hr dark: 1 hr light “long night” regimen to stimulate their pineal glands. Pinealocytes in “long night” adapted animals appeared metabolically active. Dramatic ultrastructural changes seen in pinealocytes of “long night” adapted animals after a six-hour exposure to the malodorant followed by a ten-hour period of rest included an increase in density of the nuclear chromatin, dilatations of the nuclear envelope, dilatation of the smooth endoplasmic reticulum and swelling of mitochondria. Isocyanide treatment did not affect the ultrastructure of liver, kidney, olfactory bulb or striate cortex of the animals exposed to odor. Our observations indicate that inhalation of isocyanide induces specific ultrastructural changes in the pineal gland which may be suggestive of its altered synthetic activity.  相似文献   

Nitric oxide is involved in chitosan‐induced systemic resistance in pearl millet against downy mildew disease     

Girigowda Manjunatha  Sathyanaraya Niranjan‐Raj  Geetha N Prashanth  Shantharaj Deepak  Kestur N Amruthesh  Hunthrike S Shetty 《Pest management science》2009,65(7):737-743

BACKGROUND: The nature and durability of resistance offered by chitosan and the involvement of nitric oxide (NO) in chitosan‐induced defence reactions in pearl millet against downy mildew disease were investigated. RESULTS: It had previously been reported that chitosan seed priming protected pearl millet plants against downy mildew disease. Further elucidation of the mechanism of resistance showed that chitosan seed priming protects the plants systemically. A minimum 4 day time gap is required between the chitosan treatment and pathogen inoculation for maximum resistance development, and it was found to be durable. Chitosan seed priming elevated NO accumulation in pearl millet seedlings, beginning from 2 h post‐inoculation, and it was found to be involved in the activation of early defence reactions such as hypersensitive reaction, callose deposition and PR‐1 protein expression. Pretreatment with NO scavenger C‐PTIO and nitric oxide synthase (NOS) inhibitor L‐NAME before pathogen inoculation reduced the disease‐protecting ability of chitosan, and defence reactions were also downregulated, which indicated a possible role for NO in chitosan‐induced resistance. CONCLUSION: Protection offered by chitosan against pearl millet downy mildew disease is systemic in nature and durable. Chitosan‐induced resistance is activated via NO signalling, as defence reactions induced by chitosan were downregulated under NO deficient conditions. Copyright © 2009 Society of Chemical Industry  相似文献   

Ubiquitinated TDP-43 in frontotemporal lobar degeneration and amyotrophic lateral sclerosis   总被引：4，自引：0，他引：4  

Neumann M  Sampathu DM  Kwong LK  Truax AC  Micsenyi MC  Chou TT  Bruce J  Schuck T  Grossman M  Clark CM  McCluskey LF  Miller BL  Masliah E  Mackenzie IR  Feldman H  Feiden W  Kretzschmar HA  Trojanowski JQ  Lee VM 《Science (New York, N.Y.)》2006,314(5796):130-133

Ubiquitin-positive, tau- and alpha-synuclein-negative inclusions are hallmarks of frontotemporal lobar degeneration with ubiquitin-positive inclusions and amyotrophic lateral sclerosis. Although the identity of the ubiquitinated protein specific to either disorder was unknown, we showed that TDP-43 is the major disease protein in both disorders. Pathologic TDP-43 was hyper-phosphorylated, ubiquitinated, and cleaved to generate C-terminal fragments and was recovered only from affected central nervous system regions, including hippocampus, neocortex, and spinal cord. TDP-43 represents the common pathologic substrate linking these neurodegenerative disorders.  相似文献   

Cumulative effects of tree-based intercropping on arbuscular mycorrhizal fungi     

Ashok Shukla  Anil Kumar  Anuradha Jha  Shiv Kumar Dhyani  Deepak Vyas 《Biology and Fertility of Soils》2012,48(8):899-909

In tree-based intercropping system (agroforestry), the role of perennial trees in maintaining active populations and mycelial networks of arbuscular mycorrhizal fungi (AMF) is well documented. Agroforestry positively influences the AMF community, but complete studies regarding mycorrhization in such systems are scarce. The present study was conducted to assess the effect of tree introduction in agriculture fields on mycorrhization. In particular, we investigated the effect of trees on AMF colonization of intercrops and vice versa, the effect of canopy management of trees on their root colonization, and the cross-infectivity of AMF isolated from tree rhizosphere in intercrops and vice versa. The results of the field study suggest that in agroforestry systems, trees acted as AMF inoculum reservoir for intercrops, especially during the rainy season. Intercropping (Phaseolus mungo and Triticum aestivum in the rainy and winter seasons, respectively) increased mycorrhization, i.e., root colonization and spore population in the rhizosphere of Albizia procera and Eucalyptus tereticornis. Canopy management, i.e., shoot pruning, reduces root colonization in A. procera, Anogeissus pendula, Dalbergia sissoo, Hardwickia binata, and Tectona grandis, especially in April 2005 (late spring), but during subsequent periods, differences among the treatments were at par. Results from greenhouse suggest that AMF are nonspecific in their selection of host since species isolated from tree rhizosphere could colonize the roots of crops and vice versa.  相似文献