Restoration and Rehabilitation of Mixed Espinales in Central Chile: 10-Year Report and Appraisal     

Carlos Ovalle  James Aronson  Alejandro Del Pozo  Julia Avendan 《Arid Land Research and Management》2013,27(4):369-381

A synthesis of progress achieved thus far is provided for a 10 - year research and development program aimed at the ecological and economic rehabilitation of agroecosystems in the unirrigated portions of the central valley of Chile's Mediterranean climate region. We review our data on (1) revised management techniques aimed at restoring the gross superstructure and former levels of diversity and productivity of a mixed espinales formation; (2) selection and utilization of ecotypes of the naturalized annual Medicago polymorpha L. and the N2-fixing microsymbiont Rhizobium meliloti, for gradual improvement of espinal soil fertility; and (3) studies of the outstandingly well adapted and fast growing Canary Island tree Chamaecytisus proliferus (L. fil.) Link ssp. palmensis (Christ) Kunkel (Tagasaste), which along with about three dozen other woody nitrogen - fixing legume and several nonlegume multipurpose trees, was considered to be of potential value for deep soil layer rehabilitation combined with economic improvements. In all these subprograms, efforts were made to identify techniques or organisms that could provide short-term benefits to landowners, especially in the form of wood, fuelwood, or forage for livestock. We also consider various approaches to be added to the program over the next decade, especially in view of the overarching goal of reintegrating fragmented landscapes and combining restoration with rehabilitation and reallocation at this spatial scale.  相似文献   

Perturbation of the EphA2-EphrinA1 System in Human Prostate Cancer Cells by Colonic (Poly)phenol Catabolites     

Tognolini M  Giorgio C  Hassan Mohamed I  Barocelli E  Calani L  Reynaud E  Dangles O  Borges G  Crozier A  Brighenti F  Del Rio D 《Journal of agricultural and food chemistry》2012,60(36):8877-8884

The Eph tyrosine kinase receptors and their ephrin ligands play a central role in human cancer as their deregulated expression induces tumorigenesis with aggressive phenotypes. To evaluate their potential contribution to EphA2-ephrinA1 modulation, several colonic catabolites of dietary (poly)phenolics, known to be generated in vivo, were screened using an ELISA-based binding assay. Some of the catabolites inhibited the binding in a dose-dependent manner (IC(50) values from 0.26 to 43 μM). Functional studies on prostate adenocarcinoma cells revealed that pyrogallol and protocatechuic acid specifically antagonized ephrinA1-Fc-induced EphA2 phosphorylation at concentrations that were not cytotoxic. The active concentrations of pyrogallol appear to be close to what can be reached in vivo under physiological conditions. Finally, because of the roles played by the Eph-ephrin system not only in cancer development but also in neurodegeneration and diabetes, pyrogallol and protocatechuic acid are candidates for more detailed functional studies to elucidate their role in these pathophysiological processes.  相似文献   

Rhizospheric organic compounds in the soil–microorganism–plant system: their role in iron availability     

T. Mimmo  D. Del Buono  R. Terzano  N. Tomasi  G. Vigani  C. Crecchio  R. Pinton  G. Zocchi  S. Cesco 《European Journal of Soil Science》2014,65(5):629-642

Poor iron (Fe) availability in soil represents one of the most important limiting factors of agricultural production and is closely linked to physical, chemical and biological processes within the rhizosphere as a result of soil–microorganism–plant interactions. Iron shortage induces several mechanisms in soil organisms, resulting in an enhanced release of inorganic (such as protons) and organic (organic acids, carbohydrates, amino acids, phytosiderophores, siderophores, phenolics and enzymes) compounds to increase the solubility of poorly available Fe pools. However, rhizospheric organic compounds (ROCs) have short half‐lives because of the large microbial activity at the soil–root interface, which might limit their effects on Fe mobility and acquisition. In addition, ROCs also have a selective effect on the microbial community present in the rhizosphere. This review aims therefore to unravel these complex dynamics with the objective of providing an overview of the rhizosphere processes involved in Fe acquisition by soil organisms (plants and microorganisms). In particular, the review provides information on (i) Fe availability in soils, including mineral weathering and Fe mobilization from soil minerals, ligand and element competition and plant‐microbe competition; (ii) microbe–plant interactions, focusing on beneficial microbial communities and their association with plants, which in turn influences plant mineral nutrition; (iii) plant–soil interactions involving the metabolic changes triggered by Fe deficiency and the processes involved in exudate release from roots; and (iv) the influence of agrochemicals commonly used in agricultural production systems on rhizosphere processes related to Fe availability and acquisition by crops.  相似文献   

Pain induced by a minor medical procedure (bone marrow aspiration) in dogs: comparison of pain scales in a pilot study     

Guillot M  Rialland P  Nadeau MÈ  Del Castillo JR  Gauvin D  Troncy E 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2011,25(5):1050-1056

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Plants as biofactories: physiological role of reactive oxygen species on the accumulation of phenolic antioxidants in carrot tissue under wounding and hyperoxia stress     

Jacobo-Velázquez DA  Martínez-Hernández GB  Del C Rodríguez S  Cao CM  Cisneros-Zevallos L 《Journal of agricultural and food chemistry》2011,59(12):6583-6593

Plants subjected to postharvest abiotic stresses synthesize secondary metabolites with health-promoting properties. Here, we report the potential use of carrots (Daucus carota) as biofactories of caffeoylquinic acids when subjected to wounding and hyperoxia stresses. Wounding stress induced an increase of ～287% in total phenolic content (PC) in carrots stored for 48 h at 20 °C. This increase was higher (～349%) in the wounded tissue treated with hyperoxia stress. To further understand the physiological role of reactive oxygen species (ROS) as a signaling molecule for the stress-induced accumulation of phenolics in carrots, the respiration rate as well as the enzymatic activities of NADPH oxidase, superoxide dismutase, ascorbate peroxidase, and catalase were evaluated. Likewise, shredded carrots were treated with diphenyleneiodonium chloride solution to block NADPH oxidase ROS productions, and the phenylalanine ammonia lyase activity and total PC were evaluated. Results demonstrated that ROS play a key role as a signaling molecule for the stress-induced accumulation of PC in carrots.  相似文献   

Surgical removing of an ectopic tooth in an Iceland mare     

Dicht S  Del Chicca F  Fürst A 《Schweizer Archiv für Tierheilkunde》2011,153(12):569-572

Ectopic teeth occur because of failure of the first branchial cleft to close during development and are found mostly in young horses. Such dentigerous cysts are often located at the base of the ear, forming a notable swelling with a fistula, as it was the case with the two year old Iceland mare ?Runa?. In order to confirm the diagnosis, x-ray images were taken, which is also necessary to locate the ectopic tooth correctly. While operating, the whole cystic membrane should be removed and it is important to prevent adjacent nerves and blood vessels from damage. Prognosis for complete healing after removing an ectopic tooth is excellent.  相似文献   

The 2010 AAVPT/EAVPT/ECVPT bioequivalence workshop     

Martinez M  Hunter RP  Baynes R  Bermingham E  Claxton R  Cole C  Del Castillo J  Gehring R  Harshman K  Lainesse C  Lucas A  Modric S  Robinson J 《Journal of veterinary pharmacology and therapeutics》2011,34(2):105-107

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Effect of the US3 protein of bovine herpesvirus 5 on the actin cytoskeleton and apoptosis     

Ladelfa MF  Kotsias F  Del Médico Zajac MP  Van den Broeke C  Favoreel H  Romera SA  Calamante G 《Veterinary microbiology》2011,153(3-4):361-366

The US3 protein is a unique protein kinase only present in the Alphaherpesvirinae subfamily of the herpesviruses. Studies performed with several alphaherpesviruses demonstrated that the US3 protein is involved in cytoskeleton modifications during viral infection and displays anti-apoptotic activity. However, the US3 protein of BoHV-5 has not been studied up to now. As reported for other alphaherpesviruses, our results showed that BoHV-5 US3 confers resistance against apoptosis and induces cytoskeletal reorganization leading to cell rounding, actin stress fiber breakdown and cell projections that interconnect cells. The expression of a kinase-dead version of BoHV-5 US3 showed that the anti-apoptotic activity and the induction of cell projections are kinase-dependent whereas kinase activity is not absolutely required for actin stress fiber breakdown. Besides, the kinase-dead version of US3, but not the wild type protein, was found excluded from the nucleus. These results constitute the first report on the BoHV-5 US3 functions, and highlight that there are functional differences and similarities among US3 proteins of different alphaherpesviruses.  相似文献   

Use of near infrared reflectance and transmittance coupled to robust calibration for the evaluation of nutritional value in naked oats     

Bellato S  Del Frate V  Redaelli R  Sgrulletta D  Bucci R  Magrì AD  Marini F 《Journal of agricultural and food chemistry》2011,59(9):4349-4360

A rapid accurate and precise method for simultaneous determination of β-glucan and protein content in naked oat samples, based on the coupling of near-infrared spectroscopy and chemometrics, is presented. In particular, three different spectroscopic approaches [near infrared reflectance (NIR) and transmittance (NIT) on flour and NIT on whole grains] and various spectral pretreatments were considered. To account for the possibility of outlying samples, a robust version of the PLS algorithm (namely partial robust M-regression) was used. All the models resulted as accurate as the reference methods, reflectance spectroscopy being the technique providing the best outcomes. Variable reduction by inclusion of the most relevant predictors only (as evaluated by VIP scores) resulted in simpler and, in one case, more parsimonious models, without loss in accuracy.  相似文献   

Potential diffusion of doramectin into a soil amended with female pig manure. A field experiment     

Gil-Díaz Mdel M  Pérez-Sanz A  Martín M  Lobo MC 《Journal of agricultural and food chemistry》2011,59(19):10635-10640

Doramectin is a veterinary drug used as an antihelminthic and is excreted mainly in the feces as the nonmetabolized drug. This study investigated the time profile of doramectin excretion in pig feces and the potential transfer and persistence of doramectin in the soil when the pig manure is used as an organic amendment to the soil. The concentration of doramectin in feces peaked at 143.0 ng/g in the dry feces 4 days after treatment. On day 62, the drug was still detected in the pig feces. After the land application of pig manure, the maximum concentration of doramectin in soil (ppb level) was detected 6 days after treatment. Seven months after the manure application, traces of doramectin were detected in the soil from the surface to a depth of 90 cm. Successive applications of manure from pigs treated with doramectin in a specific area could produce an accumulation of this drug in the soil.  相似文献