Antibody directed against Marek's disease-associated tumor surface antigen can be eluted from Marek's disease tumor cells.     

H K Pradhan  G C Mohanty  W Y Lee  B Patnaik 《Veterinary immunology and immunopathology》1991,29(3-4):229-238

Antibody directed against Marek's disease-associated tumor surface antigen (MATSA) was eluted from tumor cells of lymphomas and peripheral blood lymphocytes that were isolated from Marek's disease virus-infected chickens. Feather follicular Marek's disease virus (MDV) antigen could not be demonstrated with this antibody by indirect immunofluorescent (IF) staining. Monoclonal antibody directed against MATSA could completely block the activity of eluted antibody and vice versa. By indirect IF staining using eluted antibody and fluorescein isothiocyanate (FITC) labelled antichicken globulin conjugate. MATSA-bearing cells were detected in MDV infected and herpes virus of turkey (HVT) vaccinated birds. Blocking of immunoglobulin molecules present on B-cells by anti-chicken globulin is critical in this test.  相似文献   

DOES KETAMINE PROVIDE ADEQUATE VISCERAL ANALGESIA WHEN USED ALONE OR IN COMBINATION WITH ACEPROMAZINE,DIAZEPAM, OR BUTORPHANOL IN CATS?     

D.C. Sawyer  R.H. Rech  R.A. Durham 《Veterinary anaesthesia and analgesia》1991,18(Z1):381-381

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Characterization of and radioimmunoassay for canine thyroglobulin     

C. P. Verschueren  P. J. Selman  J. J. M. de Vijlder  J. A. Mol 《Domestic animal endocrinology》1991,8(4):509-519

Canine thyroglobulin (cTg) has been isolated and purified. It has similar electrophoretic patterns as Tg from other mammalian species. The main fraction had a MW of 660,000, whereas also fractions of a MW of approximately 1,300,000 (dimer) and 330,000 (subunit) were present. The iodine content was 0.8 to 1.0 % (w/w). cTg did not cross-react with antibodies against human Tg to a degree that would allow the use of a radioimmunoassay for human Tg for the determination of cTg in serum or plasma. Therefore a polyclonal antiserum was raised against cTg and a homologous radioimmunoassay was developed, which was sensitive (0.4 μg/l) and specific (cross-reactivity in cTg assay of human Tg, goat Tg, T₄, T₃, and DIT < 0.01 %).

Plasma Tg levels in normal dogs of both sexes and aged 3–15 years amounted to 192 ± 73 μg/l (mean ± SD, n=30). There was no relation between plasma Tg and T₄ levels.  相似文献   

Factors associated with Malassezia colonization in the ear canals of healthy dogs     

C. A. Reme 《Veterinary dermatology》2004,15(S1):15-15

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Recent advances in filtered-air positive-pressure (FAPP) housing for the production of disease-free chickens     

B W Mitchell  C W Beard  H W Yoder 《Avian diseases》1989,33(4):792-800

Design and performance information on a filtered-air positive-pressure (FAPP) housing system for disease-free poultry flocks is presented. The system includes many special features that result in excellent biological security, easy cleanup and maintenance, efficient control of environment, and a centralized alarm in the event of problems. The system has now housed eight flocks without any major problems. Based on its performance thus far, it should be useful as a reliable housing system for disease-free poultry.  相似文献   

A new virus disease of watercress in England1     

J. A. WALSH  C. M. CLAY  A. MILLER 《EPPO Bulletin》1989,19(3):463-470

In 1983 plants showing symptoms typical of watercress chlorotic leafspot agent (WCLSA) were found in Kent (GB). Later, plants showing similar symptoms were found in Dorset (1986) and Hampshire (1987), the main watercress-producing regions of England. These plants, unlike those infected with WCLSA, contained virus particles which were isometric with diameters of 37-38 nm. An antiserum to the virus was produced, with a titre in immunodiffusion tests of 1/128 and this gave good results in immunosorbent electron microscopy (ISEM) and ELISA tests on crude plant sap. Purified preparations of the virus reacted positively in ISEM and immunodiffusion tests with an antiserum to watercress yellow spot virus, a partially described virus occurring in France. Tests suggest that the crook root fungus Spongospora subterranea f.sp. nasturtii is probably the vector of this virus but other means of transmission cannot be excluded. Virus incidence within watercress beds varied, with little or no infection in the water inlet region of the bed where there are also low levels of crook root infection, higher levels in the central region and highest levels at the outlet region where crook root infection is also highest.  相似文献   

Effect of orally administered furazolidone on volume and sperm concentration of dwarf and non-dwarf cock semen     

B S Gehlaut  N S Mangle  I C Datta 《Archiv fuer experimentelle veterinaermedizin》1989,43(4):609-612

2 groups of 20 cocks each were selected at random from non-dwarf White Leghorn (28 weeks post-hatch) and dwarf Krishna-J (38 weeks post-hatch) genotypes. The treated groups comprised 10 White Leghorn and 10 Krishna-J cocks. The remaining birds served as controls. 8 weeks prior to furazolidone treatment, semen was collected from both control groups at regular 4-day intervals, for 4 weeks. Cocks of the treated groups of both genotypes were administered furazolidone (0.14 g/bird/day) for 7 consecutive days. Semen was collected from all cocks at regular 4-day intervals for 4 weeks. Semen from the cocks of the same group was pooled. The pooled ejaculate volume and sperm density did not differ significantly in the 2 genotypes. The semen output as well as sperm density increased along with progressive attainment of sexual maturity. Furazolidone treatment caused significant reduction in semen volume as well as sperm concentration in either genotype.  相似文献   

Book review     

A. C. Rowlands 《Veterinary research communications》1989,13(2):171-171

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Ocular pathology of caprine beta-mannosidosis     

J A Render  K L Lovell  M Z Jones  C A Wheeler 《Veterinary pathology》1989,26(5):444-446

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Insertion of a transtympanic ventilation tube for the treatment of otitis media with effusion     

C. L. Cox  R. W. T. Slack  G. J. Cox† 《The Journal of small animal practice》1989,30(9):517-519

In this paper, a case of otitis media with effusion (‘glue ear’) is described in a Cavalier King Charles spaniel. Its presentation, diagnosis and surgical management by the insertion of a trans-tympanic ventilation tube (grommet) is discussed.  相似文献