全文获取类型
收费全文 | 4145篇 |
免费 | 241篇 |
国内免费 | 508篇 |
专业分类
林业 | 711篇 |
农学 | 750篇 |
基础科学 | 263篇 |
636篇 | |
综合类 | 1158篇 |
农作物 | 243篇 |
水产渔业 | 146篇 |
畜牧兽医 | 538篇 |
园艺 | 125篇 |
植物保护 | 324篇 |
出版年
2024年 | 7篇 |
2023年 | 40篇 |
2022年 | 93篇 |
2021年 | 180篇 |
2020年 | 155篇 |
2019年 | 147篇 |
2018年 | 120篇 |
2017年 | 133篇 |
2016年 | 130篇 |
2015年 | 200篇 |
2014年 | 181篇 |
2013年 | 198篇 |
2012年 | 235篇 |
2011年 | 260篇 |
2010年 | 293篇 |
2009年 | 233篇 |
2008年 | 207篇 |
2007年 | 199篇 |
2006年 | 202篇 |
2005年 | 157篇 |
2004年 | 100篇 |
2003年 | 89篇 |
2002年 | 90篇 |
2001年 | 119篇 |
2000年 | 101篇 |
1999年 | 118篇 |
1998年 | 146篇 |
1997年 | 114篇 |
1996年 | 101篇 |
1995年 | 83篇 |
1994年 | 78篇 |
1993年 | 76篇 |
1992年 | 51篇 |
1991年 | 63篇 |
1990年 | 55篇 |
1989年 | 34篇 |
1988年 | 34篇 |
1987年 | 19篇 |
1986年 | 11篇 |
1985年 | 11篇 |
1984年 | 7篇 |
1983年 | 5篇 |
1982年 | 8篇 |
1981年 | 7篇 |
1980年 | 2篇 |
1979年 | 2篇 |
排序方式: 共有4894条查询结果,搜索用时 46 毫秒
991.
992.
993.
Effect of dietary vanadium and vitamin C on egg quality and antioxidant status in laying hens
下载免费PDF全文
![点击此处可从《Journal of animal physiology and animal nutrition》网站下载免费的PDF全文](/ch/ext_images/free.gif)
J. P. Wang K. R. He X. M. Ding Y. H. Luo S. P. Bai Q. F. Zeng Z. W. Su Y. Xuan K. Y. Zhang 《Journal of animal physiology and animal nutrition》2016,100(3):440-447
This study assessed the effect of dietary vanadium (V) and vitamin C (VC) on production performance, egg quality and antioxidant status in laying hens. A total of 360 laying hens (31‐week‐old) were randomly allotted into a 3 × 3 factorial arrangement treatments (four replicates and 10 chicks per replicate) with three levels of dietary V (0, 5 and 10 mg/kg) and three levels of vitamin C (0, 50 and 100 mg/kg) for 12 weeks. The effect of V and VC did not alter egg production, egg weight, average daily feed intake and feed conversion ratio during 1–12 week. Albumen height and Haugh unit value were linearly decreased (p < 0.001) by addition of V, whereas the effect of 100 mg/kg VC was observed to counteract (p < 0.05) this effect in V‐containing treatments during 1–12 week. Hens fed V‐containing diet laid lighter (linear effect, p < 0.05) coloured eggs (higher lightness value, lower redness and yellowness value), and the VC exerted no influence on it during 1–12 week. The serum superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH‐Px) activities, ability to inhibit hydroxyl radical, were significantly decreased, and the malondialdehyde (MDA) and V contents were increased (p < 0.05) by effect of V during 4, 8 and 12 week. The effect of VC alone and the interactive effect between VC and V were shown to increase serum (p < 0.05) SOD activity in 4 week and decrease MAD levels in 12 week. The result indicate that V decreased the egg quality and caused the oxidative stress at level of 5 mg/kg and 10 mg/kg, and the addition of 100 mg/kg vitamin C can alleviate its egg quality reduction effect and can mitigate the oxidative stress to some extent. 相似文献
994.
Impact of fitting dominance and additive effects on accuracy of genomic prediction of breeding values in layers
下载免费PDF全文
![点击此处可从《Zeitschrift für Tierzüchtung und Züchtungsbiologie》网站下载免费的PDF全文](/ch/ext_images/free.gif)
M. Heidaritabar A. Wolc J. Arango J. Zeng P. Settar J.E. Fulton N.P. O'Sullivan J.W.M. Bastiaansen R.L. Fernando D.J. Garrick J.C.M. Dekkers 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2016,133(5):334-346
Most genomic prediction studies fit only additive effects in models to estimate genomic breeding values (GEBV). However, if dominance genetic effects are an important source of variation for complex traits, accounting for them may improve the accuracy of GEBV. We investigated the effect of fitting dominance and additive effects on the accuracy of GEBV for eight egg production and quality traits in a purebred line of brown layers using pedigree or genomic information (42K single‐nucleotide polymorphism (SNP) panel). Phenotypes were corrected for the effect of hatch date. Additive and dominance genetic variances were estimated using genomic‐based [genomic best linear unbiased prediction (GBLUP)‐REML and BayesC] and pedigree‐based (PBLUP‐REML) methods. Breeding values were predicted using a model that included both additive and dominance effects and a model that included only additive effects. The reference population consisted of approximately 1800 animals hatched between 2004 and 2009, while approximately 300 young animals hatched in 2010 were used for validation. Accuracy of prediction was computed as the correlation between phenotypes and estimated breeding values of the validation animals divided by the square root of the estimate of heritability in the whole population. The proportion of dominance variance to total phenotypic variance ranged from 0.03 to 0.22 with PBLUP‐REML across traits, from 0 to 0.03 with GBLUP‐REML and from 0.01 to 0.05 with BayesC. Accuracies of GEBV ranged from 0.28 to 0.60 across traits. Inclusion of dominance effects did not improve the accuracy of GEBV, and differences in their accuracies between genomic‐based methods were small (0.01–0.05), with GBLUP‐REML yielding higher prediction accuracies than BayesC for egg production, egg colour and yolk weight, while BayesC yielded higher accuracies than GBLUP‐REML for the other traits. In conclusion, fitting dominance effects did not impact accuracy of genomic prediction of breeding values in this population. 相似文献
995.
以采集于甘肃省临泽县境内的芒颖大麦草(Hordeum jubatum)种子为材料,测定了种子的休眠率,并研究了去除种柄、预先冷冻、预先干热、硝酸钾(KNO_3)、赤霉素(GA3)、蒸馏水浸种和开水浸种7种处理方法破除休眠的效果。结果显示,参试种子的休眠率为73%~77%。预先冷冻、预先干热、KNO_3、GA3和蒸馏水浸种均能显著降低参试种子的休眠率(P0.05),但以预先冷冻和GA3溶液预湿发芽床两种处理的效果为最好。预先冷冻和GA3处理后种子的萌发率和萌发指数达到最大值,分别为92%、89%和39.80、42.99;平均萌发时间为最小值,分别为2.43和3.10d。去除种柄和开水浸种均未能有效破除种子休眠(P0.05)。综上分析认为,参试种子休眠属于轻度生理休眠。 相似文献
996.
苜蓿、小麦、玉米经济效益比较 总被引:3,自引:0,他引:3
基于甘肃省5个样本县的调研数据,对农户苜蓿(Medicago sativa)、小麦(Triticum aestivum)和玉米(Zea mays)种植的投入和产出、要素产出弹性和要素边际产出进行测算并比较。结果表明,苜蓿在三者中投入最少,玉米产出最高,而苜蓿产出最低;现阶段小麦和玉米处于规模报酬递减阶段而苜蓿处于规模报酬递增阶段,苜蓿产业有很大的上升空间;小麦和玉米各要素边际产量基本趋近于零,苜蓿种子投入和肥料投入的边际产量均远大于零,增加苜蓿种子和肥料投入可实现苜蓿增产。 相似文献
997.
茶小绿叶蝉具有虫体较小易隐蔽、行动活跃易逃脱、若虫脆弱易死亡等特点,导致室外捕虫工作量大、室内挑虫分装处理困难,是阻碍该虫抗药性研究进展缓慢的重要原因.通过采集叶蝉发生茶园嫩梢于室内水培、培育小绿叶蝉;通过调节改进的粉尘采样设备的不同采样流量收集叶蝉于带芽离心管;最后,通过联苯菊酯处理离心管内芽叶,测定管内小绿叶蝉的LC50值,校验方法的可行性.研究结果表明,田间虫口密度为1.538(头·梢-1)的茶园,室内水培7 d后可孵化出若虫至3.181(头·梢-1),收集室内叶蝉可减少室外采虫工作量.采用10 L·min-1的采虫流量,叶蝉收集速度快,且存活率为100%,改进的设备可简化挑虫难题.联苯菊酯对叶蝉的LC50为163.55 mg·L-1,毒力测定方法快速简便. 相似文献
998.
In vivo activity of cefquinome against Riemerella anatipestifer using the pericarditis model in the duck
下载免费PDF全文
![点击此处可从《Journal of veterinary pharmacology and therapeutics》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Y. Qu Y. Lu M. Sun Y. Zhang J. Zhong Z. Zeng 《Journal of veterinary pharmacology and therapeutics》2016,39(3):299-304
Cefquinome is a fourth‐generation cephalosporin with broad‐spectrum antibacterial activity, including activity against enteric gram‐negative bacilli such as Riemerella anatipestifer. The pericarditis model was used to examine the pharmacodynamic characteristics of cefquinome against R. anatipestifer. Serum levels of cefquinome following the administration of different doses were determined by LC‐MS/MS. Ducks with ca. 106 CFU/mL at the initiation of therapy were treated with cefquinome at doses that ranged from 0.0156 to 2 mg/kg of body weight/day (in 3, 6, 12, or 24 divided doses) for 24 h. The percentage of a 24‐h dosing interval that the unbound serum cefquinome concentrations exceeded the MIC (fT > MIC) were the pharmacokinetic (PK)–pharmacodynamic (PD) parameter that best correlated with efficacy (R2 86.3% for R. anatipestifer, compared with 58.9% for the area under the concentration–time curve/MIC and 10.6% for peak/MIC). A sigmoid Emax model was used to estimate the magnitudes of the %fT > MIC associated with net bacterial stasis, a 1‐log10 CFU reduction from baseline, and a 2‐log10 CFU reduction from baseline; the corresponding values were (22.5 ± 1.3) %, (35.2 ± 4.5) %, and (42.4 ± 2.7) %. These data showed that treatment with cefquinome results in marked antibacterial effects in qvivo against R. anatipestifer and that the host's immunity may also play a key role in the anti‐infective therapy process. 相似文献
999.
Yulong He Zhan Sang Yisha Zhuo Xueyi Wang Zeheng Guo Lihua He Cuiping Zeng Hanchuan Dai 《Journal of animal physiology and animal nutrition》2019,103(5):1521-1529
Pig transportation is associated with intestinal oxidative stress and results in destruction of intestinal integrity. Autophagy has been contributed to maintain cell homeostasis under stresses. The purpose of this study was to evaluate the effects of transport stress on morphology, intestinal mucosal barrier and autophagy/mitophagy levels in pig jejunum. A total of 16 finishing pigs were randomly divided into two groups. The control group was directly transported to the slaughterhouse and rested for 24 hr. The experimental groups were transported for 5 hr and slaughtered immediately. The results showed that transportation induced obvious stress responses with morphological and histological damage in jejunum accompanying with an elevated level of malondialdehyde (MDA; p < .05), endotoxin (LPS; p < .05), lactic dehydrogenase (LDH; p < .05) and a decreased level of serum superoxide dismutase (SOD; p < .05). Also, hemeoxy genase 1 (HO‐1; p < .01) as well as tight junction protein (claudin‐1 [p < .001], occludin [p < .05] and zonula occludens 1 [ZO‐1; p < 0.05]) levels were attenuated in jejunum tissue, and NADPH oxidase 1 (NOX1; p < .01) mRNA expression was up‐regulated. Further research indicated that transport stress could induce autophagy through increasing microtubule‐associated protein light chain 3 (LC3; p < .05) and autophagy‐related gene 5 (ATG5; p < .01) levels and suppressing p62 expression. Additionally, transport stress increased the protein levels of PTEN‐induced putative kinase 1 (PINK1; p < .05) and Parkin (p < .05) which was associated with mitophagy. In conclusions, transport stress could induce the destruction of intestinal integrity and involve in the intestinal mucosal barrier oxidative damage, and also contribute to activation of autophagy/mitophagy. 相似文献
1000.