Poisoning of horses by bamboo, Bambusa vulgaris     

Jos Diomedes Barbosa  Carlos Magno C. de Oliveira  Marcos Dutra Duarte  Gabriela Riet-Correa  Paulo Vargas Peixoto  Carlos Hubinger Tokarnia 《Journal of Equine Veterinary Science》2006,26(9):393-398

The clinical and pathological aspects of a neurological disease observed in 16 horses in Pará, Amazonia, Brazil, are presented. The symptoms were mainly motor incoordination, paresis of the tongue, somnolence, difficulties in apprehension, chewing and swallowing of food, as well as instability and standing with abducted members. The clinical course was subacute or chronic and in most cases was not fatal. Postmortem examination performed in one already very sick, euthanized animal, did not show significant macroscopic lesions; histopathological examination revealed slight edema and degenerative alterations of a few axons, mainly in the medulla oblongata. In all pastures where horses were affected, plenty of bamboo had been eaten, probably because of scarcity of pasture. By feeding large amounts of fresh bamboo leaves of this region, in different growing stages, to three horses (horse 1, 47 g/kg/d for 30 days; horse 2, 10 g/kg/d for 60 days; horse 3, 18 g/kg on the first day, and 31 g/kg/d for 6 more days)—the animals ate the leaves unassisted—it was possible to reproduce nervous symptoms essentially identical to those observed in the natural disease 24 to 72 hours after the first feeding of the plant. In spite of continuous administration of the plant, intensity of the clinical signs did not increase. Based on field observations and comparison of the clinical and pathological pictures seen in the natural and experimental disease, the described illness can be concluded to be caused by the ingestion of large amounts of the leaves of Bambusa vulgaris f. vulgaris.  相似文献   

Eco-friendly new products from enzymatically modified industrial lignins     

G. Sena-Martins  E. Almeida-Vara  J.C. Duarte 《Industrial Crops and Products》2008,27(2):189-195

Industrial lignins are by-products from the pulp and paper industry, as well as from other biomass-based industries. They are non-toxic, potentially of high value, inexpensive and available in large amounts. They possess highly reactive locations that can be enzymatically modified to develop new and environmentally friendly products.The oxidative enzymes produced by ligninolytic fungi are the catalysts that have mostly been used for the up-grading of these new technologies.This paper aims to provide a general picture of the variety of new and eco-friendly products that have recently been produced through enzyme-based technologies and using industrial lignins as raw materials, namely for the production of lignin-based copolymers by grafting, binders for wood composites, chelating agents, compositions for treating porous materials, coatings and paintings. In addition, it introduces fundamental aspects related to the enzymes used to modify the lignin structure to the interested readers that are not familiar with this field of research.  相似文献   

An easy,accurate and efficient procedure to create forest fire risk maps using the SEXTANTE plugin Modeler     

Lia Duarte  Ana Cláudia Teododo 《林业研究》2016,27(6):1361-1372

To prevent, detect, and protect against forest fires, forest personnel need to define rules for determining forest fire risk. In Portugal, all municipalities must annually produce forest fire risk(FFR) maps. To produce more reliable FFR maps more easily, we developed an open source model using the Modeler plugin of SEXTANTE in the program QGIS version 2.0 Dufour. The model provides all the maps involved in the FFR model(susceptibility map, hazard map, vulnerability map, economic value map,and potential loss map) and was produced according to Portuguese Forest Authority's(AFN, Autoridade Florestal Nacional) rules for determining the FFR. This model was tested for the Portuguese municipality Santa Maria da Feira, where 40 % of the total municipality area falls in the category ‘‘very high' or ‘‘high' fire risk. The ‘‘very high'fire risk area is mainly classified as broad-leaved forest and has the steepest slopes(15 %). The distance of burned areas to roads was also analyzed; the proportion of burned areas increased with increasing distance to the main roads.In addition, 92.6 % of the ‘‘high' and ‘‘very high' risk zones were located in areas with lower elevation. These results confirmed that forest fire is strongly influenced not only by environmental factors but also by anthropogenic factors. The procedure implemented here was compared with our open source application already available in QGIS and also to the same procedure implemented in GIS proprietary software. Although the results were obviously the same, the model developed here presents several advantages over the other two approaches. Besides being faster,it is easy to change the model parameters according to user needs(i.e., to the rules of different countries), and can be modified and adapted to other variables and other areas to create risk maps for different natural phenomena(e.g.,floods, earthquakes, landslides). The model is easy to use and to create risk and hazard maps rapidly in a free, open source environment that does not require any programming knowledge.  相似文献   

Valine,isoleucine, arginine and glycine supplementation of low-protein diets for broiler chickens during the starter and grower phases     

I. C. Ospina-Rojas  C. R. A. Duarte  C. Eyng  C. A. L. Oliveira  V. Janeiro 《British poultry science》2014,55(6):766-773

1. Two experiments were performed to study the supplementation of valine, isoleucine, arginine and glycine (Val, Ile, Arg, Gly) in low-protein diets for broiler chickens in the starter (1–21 d; Exp. 1) and grower (22–42 d; Exp. 2) phases.

2. A low-crude protein (CP) diet was formulated to meet the requirements of all amino acids (AA) supplied by the control diet except for Val, Ile, Arg and Gly. The other experimental diets were obtained by the isolated or combined supplementation of the studied AA in the low-CP diet.

3. Growth, serum parameters and litter characteristics were taken in both of the experiments. Carcass measurements were taken in Experiment 2.

4. In the starter and grower phases, low-CP diets without supplementation resulted in birds with a poorer weight gain and feed conversion than those of the birds that received the control diet.

5. In the starter phase, individual supplementation with Val and Gly, but not Ile and Arg, restored the weight gain of the birds, while diets with the addition of Val + Gly, Val + Ile + Arg, Val + Ile + Gly and Val + Ile + Arg + Gly restored their feed conversion.

6. In the grower phase, weight gain was re-established at the same rate as the control diet for the diets supplemented with Val + Ile, Val + Ile + Arg, Val + Ile + Gly and Val + Ile + Arg + Gly. However, the feed conversion was restored only in birds that received the diet supplemented with all studied AA.

7. The supplementation of Val and Gly in low-CP diets was sufficient to avoid adverse effects in the performance and serum parameters of broilers in the starter phase. However, birds in the grower phase required the combined supplementation of Val, Ile, Arg and Gly, to prevent compromised performance.  相似文献   

Oral Recombinant Feline Interferon-Omega as an alternative immune modulation therapy in FIV positive cats: Clinical and laboratory evaluation     

S. Gil  R.O. Leal  D. McGahie  N. Sepúlveda  A. Duarte  M.M.R.E. Niza  L. Tavares 《Research in veterinary science》2014

Recombinant-Feline Interferon-Omega (rFeIFN-ω) is an immune-modulator licensed for use subcutaneously in Feline Immunodeficiency virus (FIV) therapy. Despite oral protocols have been suggested, little is known about such use in FIV-infected cats. This study aimed to evaluate the clinical improvement, laboratory findings, concurrent viral excretion and acute phase proteins (APPs) in naturally FIV-infected cats under oral rFeIFN-ω therapy (0.1 MU/cat rFeIFN-ω PO, SID, 90 days). 11 FIV-positive cats were treated with oral rFeIFN-ω (PO Group). Results were compared to previous data from 7 FIV-positive cats treated with the subcutaneous licensed protocol (SC Group). Initial clinical scores were similar in both groups. Independently of the protocol, rFeIFN-ω induced a significant clinical improvement of treated cats. Concurrent viral excretion and APP’s variation were not significant in the PO Group. Oral rFeIFN-ω can be an effective alternative therapy for FIV-infected cats, being also an option for treatment follow-up in cats submitted to the licensed protocol.  相似文献   

Effect of dietary supplementation with an ethanolic extract of propolis on broiler intestinal morphology and digestive enzyme activity     

C. Eyng  A. E. Murakami  C. R. A. Duarte  T. C. Santos 《Journal of animal physiology and animal nutrition》2014,98(2):393-401

The present study aimed to evaluate the effect of different levels of an ethanolic extract of propolis (EEP) on broiler performance, carcass characteristics, weight of gastrointestinal organs, intestinal morphometry and digestive enzyme activity. 1020 male broiler chicks were assigned in a completely randomised experimental design to six treatments (EEP supplement levels of 0, 1000, 2000, 3000, 4000 and 5000 ppm) and five replications, and 34 birds per experimental unit. The experimental diets were administered from 1 to 21 days of age, and the birds were subsequently provided a ration based on corn and soybean meal. EEP supplementation from 1 to 7 days negatively affected (p < 0.05) the weight gain and feed intake. The proventriculus weight at 7 days exhibited a quadratic response (p < 0.05), which predicted a lower weight at a dose of 2865 ppm of the EEP. For the duodenum at 21 days of age, the response pattern (p < 0.05) predicted that birds that were fed 2943 and 3047 ppm of the EEP would exhibit an improved crypt depth and villus‐to‐crypt ratio respectively. The villus height, crypt depth and villus‐to‐crypt ratio in the jejunum and the ileum were not affected (p > 0.05). With increased EEP doses, the duodenal sucrase activity linearly decreased at 7 days of age and linearly increased in the jejunum at 21 days of age (p < 0.05), while pancreatic enzyme activity was unaffected (p > 0.05). Although the carcass and cut yields did not improve, the percentage of abdominal fat decreased (p < 0.05). The supplementation of the broiler pre‐starter diet with 1000–5000 ppm of the EEP impaired performance at this stage, most likely due to the decreased sucrase activity. However, the EEP supplementation from 3000 ppm improved intestinal morphophysiology at 21 days of age and did not affect the performance or carcass yield at 42 days of age.  相似文献   

Effect of Breeding Activity on the Microflora of the External Genitalia and in the Semen of Stallions,and the Relationship Between Micro‐organisms on the Skin and on the External Genitalia     

T Guimarães  C Miranda  M Pinto  E Silva  L Damásio  AL Costa  MJ Correia  JC Duarte  C Cosinha  G Lopes  G Thompson  A Rocha 《Reproduction in domestic animals》2014,49(6):926-933

A possible role of breeding activities in the composition of the microbial population in stallions' external genitalia (EG) and the relationship between micro‐organisms colonizing the skin of the abdomen and the ones colonizing the EG have not been studied. In experiment 1, EG microbiological samples were collected from 41 stallions used for both natural cover and semen collection (BST) and from 18 non‐breeding stallions (NBST). A higher (p < 0.05) frequency of isolation of potentially pathogenic species was found for BST. Age did not influence number of micro‐organism species isolated both in BST and NBST. In experiment 2, the microbial content of the EG and semen was compared in 23 BST. Most micro‐organisms isolated from the EG were present in semen, albeit with a numerically lower prevalence. In 7 stallions, six microbial species isolated from semen were absent from the EG cultures, suggesting contamination by the operator. In experiment 3, a numerically higher number of micro‐organism species was isolated from the EG of 31 stallions, than from their skin of the ventral abdomen in contact with the penis or from the skin of the thorax. With the sole exception of Escherichia coli, potentially pathogenic bacteria were only isolated from the EG but not from the skin. Results suggest that breeding activity increased the number of species colonizing the EG; most species isolated from the EG were also found in semen even if with a lower frequency, and additional semen contamination seemed to occur during its manipulation. Many micro‐organism species of the skin were also isolated from the penis, but independently of being or not in contact with the penis, skin did not seem to provide an adequate environment for the growth of potentially pathogenic bacteria that were isolated from EG, with the sole exception for E. coli.  相似文献   

The use of eutectic mixture of lidocaine and prilocaine in mice (Mus musculus) for tail vein injections     

John M David  Sandra Duarte Vogel  Kimberly Longo  Daniel Sanchez  Gregory Lawson 《Veterinary anaesthesia and analgesia》2014,41(6):654-659

ObjectiveTo investigate a topical local anesthesia technique as a means to prevent and/or diminish pain in mice in a laboratory setting associated with tail vein injections performed by personnel in training.Study designProspective, randomized experimental trial.AnimalsThirty six adult female, 23–28 g CD-1 mice from an in-house training colony. They were acclimated to routine training and handling classes.MethodsEutectic mixture of local anesthetics (EMLA) cream (2.5% lidocaine/2.5% prilocaine) or a bland ointment control (n = 18) was applied on the tail prior to intravenous injection. The injections were performed by novices, who had never attempted the procedure, and experienced personnel. All participants were blinded to treatment groups. Three injection attempts were allowed per animal. The mice were observed and scored by blinded evaluators for behavioral and physiological changes, including respiratory rate, vocalization, tail flick, and escape behaviors, during and after the injection.ResultsThis study demonstrates that aversive behaviors induced by lateral tail vein injection were not changed by the preemptive application of EMLA cream. The aversive behaviors associated with lateral tail vein injection were significantly affected by the number of injection attempts and the individual's experience level.Conclusions and clinical relevanceTopical EMLA cream did not reduce signs of aversive reaction to tail vein injection and thus we did not find support for its use in mouse training programs for tail vein injections.  相似文献   

Tuberculosis survey of free-ranging marsh deer (Blastocerus dichotomus) in Brazil.     

Janaina O Luna  Manoel A A Santos  Edison L Durigon  Jo?o P Araújo Júnior  José M B Duarte 《Journal of zoo and wildlife medicine》2003,34(4):414-415

Esophageal-pharyngeal fluids from 53 free-ranging marsh deer (Blastocerus dichotomus) captured for a research program in the state of Mato Grosso do Sul, Brazil, were assayed for tuberculosis. Total DNA was extracted. amplified by polymerase chain reaction using specific primers for Mycobacterium tuberculosis complex (M. tuberculosis, M. bovis, M. microti, and M. africanum), and observed by agarose gel electrophoresis stained with ethidium bromide. All samples were negative. This, along with necropsy and histopathology data, suggests that these animals are not shedding and probably do not have active disease.  相似文献   

Arabinogalactan proteins may facilitate the movement of pollen tubes from the stigma to the ovules in Actinidia deliciosa and Amaranthus hypochondriacus     

Sílvia Coimbra  Cristina Duarte 《Euphytica》2003,133(2):171-178

Sexual plant reproduction is a complex process that involves a series of interactions between the male gametophyte and the different cell types of the pistil. These interactions are believed to direct the pollen tube growth until its final target, the embryo sac. Arabinogalactan proteins are complex proteoglycans that are believed to be involved in these processes. The pistil is enriched in these highly glycosylated proteins and we provide results that show the selective presence of different AGP epitopes at the surface of the cells or in the ECM of the tissues that correspond exactly to the pollen tube growth pathway in Amaranthus hypochondriacus and Actinidia deliciosa. We also show that in Actinidia deliciosa, which is a dioecious plant with the male flowers having rudimentary ovaries where fertilization does not occur, there is no presence at all of the epitopes recognised by the monoclonal antibodies utilized in this study. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献