Evaluation of soybean germplasm conserved in NIAS genebank and development of mini core collections     

Akito Kaga  Takehiko Shimizu  Satoshi Watanabe  Yasutaka Tsubokura  Yuichi Katayose  Kyuya Harada  Duncan A. Vaughan  Norihiko Tomooka 《Breeding Science》2012,61(5):566-592

Genetic variation and population structure among 1603 soybean accessions, consisted of 832 Japanese landraces, 109 old and 57 recent Japanese varieties, 341 landrace from 16 Asian countries and 264 wild soybean accessions, were characterized using 191 SNP markers. Although gene diversity of Japanese soybean germplasm was slight lower than that of exotic soybean germplasm, population differentiation and clustering analyses indicated clear genetic differentiation among Japanese cultivated soybeans, exotic cultivated soybeans and wild soybeans. Nine hundred ninety eight Japanese accessions were separated to a certain extent into groups corresponding to their agro-morphologic characteristics such as photosensitivity and seed characteristics rather than their geographical origin. Based on the assessment of the SNP markers and several agro-morphologic traits, accessions that retain gene diversity of the whole collection were selected to develop several soybean sets of different sizes using an heuristic approach; a minimum of 12 accessions can represent the observed gene diversity; a mini-core collection of 96 accession can represent a major proportion of both geographic origin and agro-morphologic trait variation. These selected sets of germplasm will provide an effective platform for enhancing soybean diversity studies and assist in finding novel traits for crop improvement.  相似文献   

Enabling Conditions for Successful Community Forest Enterprises   总被引：1，自引：1，他引：0  

Duncan Macqueen 《Small-Scale Forestry》2013,12(1):145-163

In search of sustainable forest landscapes that reduce poverty and mitigate climate change, many countries have adopted a forest policy model that encourages community forest enterprise (CFE). This paper draws on international experience from the Forest Connect alliance, involving teams supporting small forest enterprises in 12 countries with more than 800 associate members from 60 countries. The hypothesis emerging from this alliance is that three main enabling conditions are required for successful CFEs: accessible commercial forest rights; processes of enterprise-oriented social organisation; and infusion of competitive business skills. Having established criteria and indicators of successful CFE, this paper critically examines a series of eight national and sub-national case studies (for Brazil, China, Ethiopia, Ghana, Guatemala, Laos, Mozambique and Nepal) to test this central hypothesis. Findings demonstrate a clear association between the implementation of these three enabling conditions and indicators of successful CFE. Examples from Dolakha and Sindhupalchowk (Nepal) and Monapo (Mozambique) illustrate the importance of ensuring these conditions. The paper concludes by drawing a number of policy implications about how to foster the enabling conditions necessary for successful CFE.  相似文献   

Muller and Kirk's Small Animal Dermatology     

Duncan Graham BVSc  BSc 《New Zealand veterinary journal》2013,61(4)

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Enzymatic transformation of humic substances by NDO     

Samer AbuBakr  Simone L. Macmil  Mark A. Nanny  Kathleen E. Duncan 《Soil biology & biochemistry》2008,40(8):2055-2062

Enzymatic transformation of humic acids (HA), fulvic acids (FA) and indole was examined using naphthalene 1,2-dioxygenase (NDO). NDO was used as a model for dioxygenase enzymes found in various microbial species. Indole was used as a model substrate for NDO-catalyzed reactions resulting in condensation products. Although NDO is not classified as a soil enzyme, all HA and FA tested were susceptible to NDO-induced transformation. The extent of NDO-specific NADH oxidation in solutions containing HA and FA paralleled the percent aromaticity of the HA and FA. Furthermore, the UV–Vis absorptive properties of NDO-treated HA and FA were altered in a manner suggesting condensation reactions similar to the formation of indigo from indole. Condensation reactions were enhanced in NDO-treated mixtures containing indole and an FA. NDO retained activity for 2 weeks under ambient conditions, and retained some enzymatic activity for 9 days based on detection of specific metabolites by HPLC, suggesting prolonged extracellular activity. Humic substances have not previously been known to be substrates for dioxygenases; even more significant was that dioxygenase enzymes can facilitate condensation reactions between indole-like functional groups well-known to be present in HA and FA. These results illustrate how dioxygenases can be potential humic-modifying enzymes when released into the environment upon microbial death and concurrent cell lysis which could alter the bioavailability of organic contaminants associated with dissolved organic matter through specific modulation of enzyme activity involving substrate competition.  相似文献   

Bartonella-associated meningoradiculoneuritis and dermatitis or panniculitis in 3 dogs     

Cross JR  Rossmeisl JH  Maggi RG  Breitschwerdt EB  Duncan RB 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2008,22(3):674-678

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Evaluation of wraps covering the distal aspect of pelvic limbs for prevention of bacterial strike-through in an ex vivo canine model     

Vince KJ  Lascelles BD  Mathews KG  Altier C  Roe SC 《Veterinary surgery : VS》2008,37(4):406-411

Objective— To determine differences in bacterial strike-through for materials commonly used to cover the distal aspect of the pelvic limb during operative site preparation.
Study Design— Randomized block design; ex vivo model.
Animals— Canine cadaveric pelvic limbs (n=40).
Methods— Pelvic limbs (n=40) were randomly assigned to 4 treatment groups: Group 1=Vetrap^™+sterile Coban^™; Group 2=latex glove+Vetrap^™+sterile Coban^™; Group 3=latex glove+Vetrap^™+sterile Coban^™+sterile latex glove+sterile Coban^™; and Group 4=latex glove+Vetrap^™+sterile disposable drape+sterile Coban^™. Limbs were contaminated with a standardized bacterial solution and routinely prepared using the assigned distal leg wrap. Bandages were fluid challenged with a saline (0.9% NaCl) solution-soaked laparotomy sponge for 30 seconds. The wrap surface was sampled for microbial culture before surgical preparation, immediately after, and 60 minutes after applying a sterile leg wrap.
Results— Bacterial growth occurred in all Group 1 cultures, 90% of Group 2 cultures, and none of the Group 3 and 4 cultures, 60 minutes after applying the sterile wrap.
Conclusion— A distal leg wrap of Vetrap^™+sterile Coban^™ is not effective in preventing bacterial strike-through.
Clinical Relevance— If similar results occur in the live animal, then a sterile impermeable barrier must be incorporated into the distal leg wrap to prevent bacterial strike-through.  相似文献   

Evaluation of the recognition of Theileria parva vaccine candidate antigens by cytotoxic T lymphocytes from Zebu cattle     

Akoolo L  Pellé R  Saya R  Awino E  Nyanjui J  Taracha EL  Kanyari P  Mwangi DM  Graham SP 《Veterinary immunology and immunopathology》2008,121(3-4):216-221

East Coast fever (ECF) is a highly fatal lymphoproliferative disease of cattle caused by Theileria parva, a tick-borne intracellular apicomplexan parasite. Parasite antigens that are targets of protective cytotoxic T lymphocyte (CTL) responses are required to formulate a sub-unit vaccine against ECF. A number of CTL target antigens have recently been identified and initial evaluation has shown their vaccine potential. This study aimed to evaluate whether these antigens were recognised by CTL obtained from six genetically diverse Zebu cattle immunized with a cocktail of T. parva stocks. T. parva Muguga specific polyclonal CD8(+) CTL lines were generated and confirmed to specifically lyse autologous infected cells. CTL recognition of autologous skin fibroblasts (iSF) transduced with recombinant modified vaccinia virus Ankara strain (MVA) expressing previously identified T. parva Muguga vaccine candidate antigens was evaluated using an IFN-gamma ELISpot assay. CTL lines from one of the four calves, BY120, responded specifically to cells infected with MVA expressing the antigen Tp2 and synthetic peptides were employed to map a new CTL epitope on this antigen. Immunoscreening of the T. parva genome with these CTL lines should identify novel antigens that will constitute valuable additions to the vaccine candidates currently being evaluated.  相似文献   

Body temperature,activity patterns and hunting in free‐living cheetah: biologging reveals new insights     

Robyn S. HETEM  Duncan MITCHELL  Brenda A. DE WITT  Linda G. FICK  Shane K. MALONEY  Leith C. R. MEYER  Andrea FULLER 《Integrative zoology》2019,14(1):30-47

As one of the few felids that is predominantly diurnal, cheetahs (Acinonyx jubatus) can be exposed to high heat loads in their natural habitat. Little is known about long‐term patterns of body temperature and activity (including hunting) in cheetahs because long‐term concurrent measurements of body temperature and activity have never been reported for cheetahs, or, indeed, for any free‐living felid. We report here body temperature and locomotor activity measured with implanted data loggers over 7 months in 5 free‐living cheetahs in Namibia. Air temperature ranged from a maximum of 39 °C in summer to ?2 °C in winter. Cheetahs had higher (～0.4 °C) maximum 24‐h body temperatures, later acrophase (～1 h), with larger fluctuations in the range of the 24‐h body temperature rhythm (approximately 0.4 °C) during a hot‐dry period than during a cool‐dry period, but maintained homeothermy irrespective of the climatic conditions. As ambient temperatures increased, the cheetahs shifted from a diurnal to a crepuscular activity pattern, with reduced activity between 900 and 1500 hours and increased nocturnal activity. The timing of hunts followed the general pattern of activity; the cheetahs hunted when they were on the move. Cheetahs hunted if an opportunity presented itself; on occasion they hunted in the midday heat or in total darkness (new moon). Biologging revealed insights into cheetah biology that are not accessible by traditional observer‐based techniques.  相似文献   

Ex vivo evaluation of a novel surgical guide on the accuracy of closing wedge osteotomies     

Shelly K. Shamir  Jakob G. Wolynski  Colleen G. Duncan  Christian Puttlitz  Felix M. Duerr 《Veterinary surgery : VS》2019,48(8):1429-1436

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Development of a comprehensive protein microarray for immunoglobulin E profiling in horses with severe asthma     

Samuel White  Meriel Moore‐Colyer  Eliane Marti  Laurent Coüetil  Duncan Hannant  Eric A. Richard  Marcos Alcocer 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(5):2327-2335

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