Down Under     

Ed Squires  Dr. 《Journal of Equine Veterinary Science》2008,28(4):202

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The Integrated Plan     

Ed SquiresJames A. Orsini  DVM  ACVS 《Journal of Equine Veterinary Science》2008

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A novel strategy for resynchronization of ovulation in Nelore cows using injectable progesterone (P4) and P4 releasing devices to perform two timed inseminations within 22 days     

Guilherme Pugliesi  Danilo Zago Bisinotto  Barbara Piffero Mello  Fbio C. Lahr  Catia A. Ferreira  Gabriela Dalmaso Melo  Michele Ricieri Bastos  Ed Hoffmann Madureira 《Reproduction in domestic animals》2019,54(8):1149-1154

We aimed to evaluate the reproductive performance of Nelore lactating cows submitted to a resynchronization 12 days after timed artificial insemination (TAI) with or without a long‐acting progesterone (P4‐LA) treatment. Nelore cows were submitted to a P4/oestradiol‐based TAI protocol (D0 = insemination). On D12, cows in the control group (n = 184) received a new P4 intravaginal device (0.96 g), whereas cows in the P4‐LA group (n = 192) received the P4 device and 75 mg P4‐LA. Cows identified as non‐pregnant (n = 120) by regression of corpus luteum using colour Doppler ultrasonography on D20 had the P4 device removed and received 500ug of sodium cloprostenol, 1 mg of oestradiol cypionate and 300 IU of eCG and were re‐inseminated on D22. There was no difference (p > 0.10) in the pregnancy rate at D20, D30 and D60 after first TAI between the control (69%, 59.7% and 57%, respectively) and P4‐LA (67%, 55.7%, and 55.2%, respectively) groups. Pregnancy losses were similar between both groups (p > 0.1). For cows submitted to the second TAI, the pre‐ovulatory follicle size did not differ (p > 0.1), but the oestrous detection and pregnancy rates were greater (p < 0.05) in the P4‐LA group (92.2% [59/64] and 60.9% [39/64], respectively) than in controls (75% [42/56] and 44.6% [25/56]). The cumulative pregnancy rate after two TAIs did not differ (p > 0.1) between control (73.3% [135/184]) and P4‐LA (76% [146/192]) groups. The use of P4‐LA at 12 days after TAI potentially increases the pregnancy rates for a new early resynchronization strategy associated with the Doppler imaging for pregnancy diagnosis and results in an alternative to perform two TAIs in 22 days in beef cows.  相似文献   

Comparative Evaluation of 2 In-Clinic Assays for Vector-Borne Disease Testing in Dogs     

Jiayou Liu  Jan Drexel  Blaine Andrews  Matt Eberts  Ed Breitschwerdt  Ramaswamy Chandrashekar 《Topics in companion animal medicine》2018,33(4):114-118

Vector-borne agents comprise medically important infections affecting dogs throughout much of the world. Sensitive detection of antibodies directed at tick-borne disease-causing organisms in dogs is diagnostically important for veterinarians, pets and their owners, and epidemiologically important for public health surveillance. The SNAP 4Dx Plus Test (IDEXX Laboratories, Inc., Westbrook, ME) identifies antibodies to or infection with multiple tick-borne pathogens and canine heartworm antigen in a single assay. Recently, VetScan FLEX4 Rapid Test (Abaxis, Inc., Union City, CA) was launched as a new assay to detect tick-borne pathogen antibodies and heartworm antigen. In the present study, we evaluated the comparative performance of SNAP 4Dx Plus (SNAP) and FLEX4 Rapid Test (FLEX4) using samples selected based on geographic distributions for canine vector borne diseases, including Borrelia burgdorferi (n = 105), Anaplasma phagocytophilum (160), Anaplasma platys (115), Ehrlichia canis (154), Ehrlichia ewingii (163), Ehrlichia chaffeensis (151) and Dirofilaria immitis (105). Canine vector borne diseases infection status was established for each sample by a combination of reference methods that included necropsy (D. immitis, heartworm disease), Western immunoblotting (B. burgdorferi), immunofluorescence assays (A. phagocytophilum and E. canis) and species-specific ELISAs (A. platys, E. canis, E. ewingii and E. chaffeensis). For comparisons among the 2 assays, samples were evaluated per the manufacturers’ instructions for each test kit.By testing each same sample set compared to the defined reference results, sensitivities differed substantially between SNAP and FLEX4, at 95.5 vs. 40.9%, respectively for B. burgdorferi, 97.1% vs. 61.4% for E. canis, 98.2% vs. 59.3% for E. ewingii, 64.3% vs. 35.7% for E. chaffeensis, 84.5% vs. 12.7% for A. phagocytophilum, 83.3% vs. 33.3% for A. platys, and 94.1% vs. 88.2% for D. immitis. Specificities for both rapid assay tests ranged from 98% to 100%.Based upon the comparative results derived from this study, the SNAP test was more sensitive than the FLEX4 test for detection of antibodies to all tick-borne pathogens and heartworm disease (Dirofilaria immitis) antigen in dogs.  相似文献   

An investigation of the effects of intratesticular alfaxalone and lidocaine during castration in piglets     

Trina M. Hancock  Nigel A. Caulkett  Ed A. Pajor  Leanna Grenwich 《Veterinary anaesthesia and analgesia》2018,45(6):858-864

Objective

To determine whether intratesticular injection of an alfaxalone–lidocaine combination can induce anesthesia and provide a rapid recovery in piglets undergoing surgical castration.

Study design

Randomized experimental study.

Animals

A group of 30 male piglets, aged 2–10 days, weighing 1.3–4.6 kg.

Methods

Animals were randomly divided into three equal groups for intratesticular administration of alfaxalone + lidocaine: high dose (group HD; 8 mg kg^–1 + 2.5 mg kg^–1), medium dose (group MD; 6 mg kg^?1 + 2 mg kg^?1) and low dose (group LD; 4 mg kg^?1 + 1.5 mg kg^?1). Induction and recovery times, movement and vocalization were recorded. Pulse rate (PR), oxygen saturation, respiratory rate (f_R), rectal temperature, blood pressure and end-tidal carbon dioxide were recorded until recovery.

Results

Induction time did not differ significantly among groups (p = 0.19); mean time of 2.2, 3.3 and 3.7 minutes for HD, MD and LD, respectively. Recovery time to sternal recumbency was significantly faster in LD compared with HD and MD (p = 0.005). Time to standing was mean 34.1, 31.6 and 29.6 minutes for HD, MD and LD, respectively (p = 0.58). Incidences of movement and vocalization during the castration procedure were decreased in HD and MD compared with LD, but were not statistically different. There were no differences in the physiologic data among the groups except for PR, which decreased in all three groups (p < 0.05), and f_R, which increased in MD and LD (p < 0.05).

Conclusions and clinical relevance

The alfaxalone–lidocaine combinations investigated in this study induced deep sedation in all piglets. Physiologic data remained within clinically acceptable ranges, suggesting that this drug combination by intratesticular injection prior to castration in neonatal piglets is well tolerated. The authors recommend the alfaxalone (6 mg kg^?1) + lidocaine (2 mg kg^?1) dose.  相似文献   

Recreational sandboxes for children and dogs can be a source of epidemic ribotypes of Clostridium difficile          下载免费PDF全文

Cristina Orden  Carlos Neila  José L. Blanco  Sergio Álvarez‐Pérez  Celine Harmanus  Ed J. Kuijper  Marta E. García 《Zoonoses and public health》2018,65(1):88-95

Different studies have suggested that the sand of public playgrounds could have a role in the transmission of infections, particularly in children. Furthermore, free access of pets and other animals to the playgrounds might increase such a risk. We studied the presence of Clostridium difficile in 20 pairs of sandboxes for children and dogs located in different playgrounds within the Madrid region (Spain). Clostridium difficile isolation was performed by enrichment and selective culture procedures. The genetic (ribotype and amplified fragment length polymorphism [AFLP]) diversity and antibiotic susceptibility of isolates was also studied. Overall, 52.5% (21/40) of samples were positive for the presence of C. difficile. Eight of the 20 available isolates belonged to the toxigenic ribotypes 014 (n = 5) and 106 (n = 2), both regarded as epidemic, and CD047 (n = 1). The other 12 isolates were non‐toxigenic, and belonged to ribotypes 009 (n = 5), 039 (n = 4), and 067, 151 and CD048 (one isolate each). Nevertheless, all isolates (even those of a same ribotype) were classified into different AFLP genotypes indicating non‐relatedness. In conclusion, our results revealed the presence of epidemic ribotypes of C. difficile in children's and dog's sandboxes located nearby, which constitutes a major health risk.  相似文献   

Population structure and regeneration of multiple-use tree species in a semi-deciduous African tropical rainforest: Implications for primate conservation     

Edward N. Mwavu  Ed T.F. Witkowski 《Forest Ecology and Management》2009

The conservation of threatened frugivorous primates (e.g. chimpanzees) and birds in the Albertine Rift Ecoregion rainforests requires the conservation of food tree species aided by an understanding of their natural regeneration and population trends. However, little is known of the population structure and regeneration patterns of a wide range of multiple-use tree species in many of these forests.  相似文献   

Promoter anchored amplified polymorphism based on random amplified polymorphic DNA (PAAP-RAPD) in cotton   总被引：1，自引：0，他引：1  

Mingxiong Pang  R. G. Percy  Ed. Hughs  Jinfa Zhang 《Euphytica》2009,167(3):281-291

Non-coding sequences account for a majority of the higher plant genome, some of which have important effects in gene regulation and plant development. In an effort to develop molecular marker systems to search for polymorphisms associated with high fiber yield and quality in cotton, we have developed a methodology that could specifically target the regulatory regions of the cotton genome. In this study we designed 10-nucleotide degenerate promoter primers based on conserved core promoter sequences and tested their applicability in PCR amplifications in combination with 10-mer random amplified polymorphic DNA (RAPD) primers. The amplified markers are called promoter anchored amplified polymorphism based on RAPD (PAAP-RAPD). Forty cotton genotypes with diverse genetic and geographical backgrounds were used to test the PAAP-RAPD system using polyacrylamide gel electrophoresis. Based on PAAP-RAPD markers amplified from 12 primer combinations, the 40 genotypes were classified into five distinctive groups: two Upland cotton (Gossypium hirsutum) groups from China, another two Upland cotton groups from the USA, and one group from American Pima cotton (G. barbadense). The groupings are in general consistent with their genetic and geographical origins. Thirty-six PAAP-RAPD and RAPD fragments were cloned and four of them were further subjected to sequence analysis. Signal scanning using software PLACE confirmed that they contained an array of cis-regulatory sequences in addition to the core promoter sequences. The results demonstrate the potential application of PAAP-RAPD as a new marker system specifically targeting regulatory regions of the plant genome.  相似文献   

The Potato Association of America     

Steven R. James  Lori Wing  Ronald Knight  Barbara Harrity  Devid H. Lambert  Bud Platt  Al Reeves  Bill Bohl  Lind Sanford  Alexander D. Pavlista  Ed Plissey  Richard Sawyer  Joe Guenthner  Willem Schrage  Edward B. Radcilffe  Nora Olsen  Jeff Miller  Rikki Sterrett  Gale W. Harding  Marcin Topoleweki 《American Journal of Potato Research》2000,77(6):369-390

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Beobachtungen über Dicerca aenea (Buprestis), meffingfarbiger Spikprachtfäfer     

Ed. Afterberg 《European Journal of Forest Research》1860,4(1):439-441

Ohne Zusammenfassung  相似文献