Phenethyl isothiocyanate as an anti-nutritional factor attenuates deoxynivalenol-induced IPEC-J2 cell injury through inhibiting ROSmediated autophagy     

Shuiping Liu  Xinru Mao  Lei Ge  Lili Hou  Guannan Le  Fang Gan  Lixin Wen  Kehe Huang 《动物营养（英文）》2022,8(1):300

Deoxynivalenol(DON)is considered to be the most harmful mycotoxin that affects the intestinal health of animals and humans.Phenethyl isothiocyanate(PEITC)in feedstuff is an anti-nutritional factor and impairs nutrient digestion and absorption in the animal intestinal.In the current study,we aimed to explore the effects of PEITC on DON-induced apoptosis,intestinal tight junction disorder,and its potential molecular mechanism in the porcine jejunum epithelial cell line(IPEC-J2).Our results indicated that PEITC treatment markedly alleviated DON-induced cytotoxicity,decreasing the apoptotic cell percentage and pro-apoptotic mRNA/protein levels,and increasing zonula occludens-1(ZO-1),occludin and claudin-1 mRNA/protein expression.Meanwhile,PEITC treatment ameliorated DON-induced an increase of the inducible nitric oxide synthase(iNOS)and cyclooxygenase 2(COX-2)mRNA levels and intracellular reactive oxygen species(ROS)level,and a decrease of glutathione peroxidase 1(GPx1),superoxide dismutase 2(SOD2),catalase(CAT)and heme oxygenase 1(HO-1)mRNA levels.Additionally,PEITC treatment significantly down-regulated autophagy-related protein 5(ATG5),beclin-1 and microtubuleassociated protein 1 light chain 3B(LC3-II)mRNA/protein levels,decreased the number of green fluorescent protein-microtubule-associated protein 1 light-chain 3(GFP-LC3)puncta and phosphatidylinositol 3 kinase(PI3K)protein expression,and up-regulated phospho-protein kinase B(p-Akt)and phospho-mammalian target of rapamycin(p-mTOR)protein expression against DON.However,the activation of autophagy by rapamycin,an autophagy agonist,abolished the protective effects of PEITC against DON-induced cytotoxicity,apoptosis and intestinal tight junction disorder.Collectively,PEITC could confer protection against DON-induced porcine intestinal epithelial cell injury by suppressing ROSmediated autophagy.  相似文献   

不同酶制剂对水稻秸秆和白酒糟混合青贮品质的影响   总被引：1，自引：0，他引：1  

寇江涛  程钰  张海波  郭冬生  雷佳星  孟佳琪  刘鑫  李乐 《草地学报》2021,29(2):396-401

为研究不同酶制剂对水稻(Oryzasativa L.)秸秆和白酒糟(m︰m=1︰1)混合青贮品质的影响,设置CK(对照)、CEL(250 U·g-1纤维素酶)和XYL(250 U·g-1木聚糖酶)3个处理,青贮120 d后取样测定其青贮品质.结果表明:CEL处理的感官评定等级为良好;CEL和XYL处理的干物质、粗脂肪、...  相似文献   

GA3介导miR171s及其靶基因VvSCLs调控葡萄种子发育的作用分析     

王文然  解振强  诸葛雅贤  白云赫  管乐  吴伟民  张培安  郑婷  房经贵  王晨 《中国农业科学》2021,54(2):357-369

[目的]鉴定vvi-miR171s成员及其靶基因,明确vvi-miR171s成员及其靶基因在葡萄种子发育中的重要作用及其响应赤霉素(Gibberellin,GA)调控种子发育的表达模式.[方法]以'魏可'葡萄('Wink')果实为试材,利用miR-RACE技术鉴定vvi-miR171a/b/e/f/g/h的成熟体序列;...  相似文献   

球孢白僵菌生物学性状与对玉米螟毒力相关性分析     

李乐  路杨  赵宇  李启云  徐文静  张正坤  毛刚 《玉米科学》2021,29(1):162-169

球孢白僵菌的生物学性状与对靶标害虫的毒力具有显著相关性,对球孢白僵菌高毒力菌株的初步筛选具有重要意义.鉴定随机分离自亚洲玉米螟幼虫僵虫的10个球孢白僵菌菌株,对其进行产孢量、孢子萌发率、菌落生长速度以及对亚洲玉米螟幼虫的毒力测定.结果表明,不同菌株的生物学特性和毒力均呈现显著差异.在此基础上,分别进行不同菌株生物学性状...  相似文献   

头孢氨苄对斑节对虾幼体发育及育苗水体细菌数量的影响     

马之明  周发林  黄建华  叶乐  江世贵 《南方水产》2005,1(4):36-40

研究了不同浓度头孢氨苄(Cefalexin)对斑节对虾Penaeusmonodon幼体发育以及对斑节对虾育苗水体中异养菌总数和弧菌数量的影响。在试验初期,无论是对照组还是施用头孢氨苄的各试验组,育苗水体中的总异养菌数量和弧菌数量都呈现出较为明显的上升趋势;在第二次施用头孢氨苄后,各试验组的异养菌总数和弧菌数量的增幅明显低于对照组(或出现异养菌总数和弧菌数量缓慢下降),而对照组异养菌总数和弧菌数量则呈现显著的上升。施用0.5～1.0μL·L-1头孢氨苄对育苗水体中的总异养菌有显著的杀灭及抑制作用,但对弧菌数量杀灭及抑制作用并不显著;施用1.5μL·L-1头孢氨苄对育苗水体中的总异养菌和弧菌数量的杀灭及抑制作用显著。当幼体变态发育至仔虾第8天(P8)时,对照组异养菌总数和弧菌数量分别达6.33×105和3.55×104CFU·mL-1,均远远高于试验组的异养菌总数和弧菌数量增长的幅度。试验组的幼体变态到P1期的时间,比对照组缩短了10h以上;试验组的成苗率也比对照组提高了3.2%～13.1%。  相似文献   

两种杂交石斑鱼子一代杂种优势的微卫星标记分析   总被引：2，自引：3，他引：2  

周翰林  张勇  齐鑫  张海发  张磊  王乐  蒙子宁  刘晓春  林浩然 《水产学报》2012,36(2):161-169

使用6对微卫星引物对两种杂交子一代(青龙斑和虎龙斑)及其亲本(斜带石斑鱼、棕点石斑鱼和鞍带石斑鱼)共5个群体进行微卫星分析,计算等位基因频率、有效等位基因数、基因杂合度、多态信息含量、相似指数和Nei氏遗传距离。结果显示,在5种石斑鱼中,棕点石斑鱼的平均有效等位基因数最大(6.849 3),最小的是鞍带石斑鱼(2.608 6)。6对微卫星引物在这5个群体中具有丰富的多态性,平均多态信息含量(PIC)分别为0.666 7、0.751 3、0.441 9、0.664 0、0.542 6。在平均观测杂合度(Ho)中,虎龙斑和青龙斑的最高均为0.940 0,鞍带石斑鱼的最低为0.508 3。遗传距离和遗传相似率结果显示,青龙斑和虎龙斑均与父本鞍带石斑鱼的亲缘关系较近。杂交子一代群体的等位基因基本来自父母本群体双方,可推断杂交子一代的遗传物质来自父母双方,属两性融合生殖,是真正意义上的杂交种。杂交后代的遗传变异水平明显增强,这是杂种优势得以形成的重要遗传物质基础。  相似文献   

我国水产养殖保险发展的几点建议     

王莉  骆乐 《渔业经济研究》2006,(1):32-35

水产养殖业是风险较大的产业,水产养殖者在水产养殖的过程中,难免遭受自然灾害和意外事故,保险机构如能对水产养殖者所遭受的经济损失及时提供经济保障,必将为水产养殖业的稳定发展起到重要作用。本文对中国水产养殖保险发展现状、必要性及存在的问题等进行了阐述,并提出一些建议。  相似文献   

Most fish destined for fishmeal production are food‐grade fish          下载免费PDF全文

Tim Cashion  Frédéric Le Manach  Dirk Zeller  Daniel Pauly 《Fish and Fisheries》2017,18(5):837-844

Marine fisheries target and catch fish both for direct human consumption (DHC) as well as for fishmeal and fish oil, and other products. We derived the fractions used for each for 1950–2010 by fishing country, and thus provide a factual foundation for discussions of the optimal use of fisheries resources. From 1950 to 2010, 27% (~20 million tonnes annually) of globally reconstructed marine fisheries landings were destined for uses other than DHC. Importantly, 90% of fish destined for uses other than DHC are food‐grade or prime food‐grade fish, while fish without a ready market for DHC make up a much smaller proportion. These findings have implications for how we are using fish to feed ourselves or, more appropriately, how we are not using fish to feed ourselves.  相似文献   

Effects of Thyroxin and Domperidone on Oocyte Maturation and Spawning Performances in the Rabbit Fish,Siganus guttatus          下载免费PDF全文

Hung Q. Pham  Hoang M. Le 《Journal of the World Aquaculture Society》2016,47(5):691-700

Thyroxin (T₄) is proven to have various functions in fish reproduction. In this study, we investigated oocyte maturation and spawning performance in the rabbit fish, Siganus guttatus, orally exposed to different doses of T₄ (0.05, 0.25, and 0.5 mg/kg fish) during vitellogenesis. No statistically significant differences of reproductive characteristics were observed among the treatments. However, the group that was exposed to the dose of 0.5 mg/kg spawned after receiving a hormone injection. The findings suggest that feeding T₄ during vitellogenesis did not advance oocyte maturation, but probably could induce spawning in rabbit fish. In a separate experiment, we investigated the effects of orally administered domperidone (DOM) on reproductive performances of the female rabbit fish. The study showed that oral administration of DOM during the early stages of the reproductive cycle had no significant effects on oocyte maturation and spawning performance. Overall, these findings suggest that DOM may not be needed for the induction of maturation and spawning of this species under aquaculture conditions. These practical investigations were conducted with a view to attempt reducing drug use, production costs, and support sustainable aquaculture.  相似文献   

Study on the Stability of Blue-tongue Virus after Long Time Storage in Cold Room     

MIAO Hai-sheng  LI Le  ZHU Jian-bo  XIAO Lei  LI Hua-chun 《中国畜牧兽医》2013,40(6):41-44

In order to understand the stability of blue-tongue virus (BTV)storage in cold room for long time, 18 tissue samples which were kept in 4℃ for 10 to 15 years were test by Real-time PCR, and 10 blood samples which were kept in 4℃ for 15 years were subjected to virus isolation process, then was proved by monoclonal antibody staining,research results showed all old samples were positive in Real-time PCR, further more, 5 live virus were isolated from 10 old blood samples. The results suggested the stability of BTV was higher than we thought before.  相似文献