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Proteins from pollen and leaves of parent forms and of a hybrid and an amphiploid Aegilops geniculata × S. cereale were subjected to a study by two-dimensional gel electrophoresis (2-DE). The majority of peptides found in the parents were
revealed in the amphiploid pollen and the hybrid and amphiploid leaves, however, numerous parental peptides were absent both
in hybrid and amphiploid. There were detected characteristic peptides in leaves of the hybrid as well as in the leaves and
pollen of the amphiploid, which were absent in the material from both parents. 52 peptides were common for pollen and leaves
of the amphiploid what corresponds to 14.2% and 25.7% of peptides in pollen and leaves, respectively. Three characteristic
peptides were common for leaves and pollen of the amphiploid (MW 48.0 kDa in pH 5.9, MW 49.0 kDa in pH 6.9 and MW 22.0 kDa
in pH 8.8).
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
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Wojciechowska JI Hewson CJ Stryhn H Guy NC Patronek GJ Timmons V 《American journal of veterinary research》2005,66(8):1453-1460
OBJECTIVE: To develop a preliminary discriminative questionnaire for assessment of nonphysical aspects of the quality of life (QOL) of pet dogs and evaluate the questionnaire's content validity, test-retest reliability, and internal consistency. STUDY POPULATION: Owners of 120 dogs. PROCEDURE: Each QOL question had 4 response options, representing descending levels of QOL that were equally weighted. A maximum of 38 items contributed to the QOL score. The questionnaire was administered by telephone to owners of dogs with appointments at a veterinary teaching hospital before the appointment. After the appointment, each dog was classified as sick or healthy by use of defined criteria; owners of healthy dogs had a second interview 3 to 4 weeks later. Test-retest reliability (kappa), internal consistency (Cronbach alpha), and respondents' comprehension were used as criteria for excluding an item. RESULTS: There were 77 sick and 43 healthy dogs. Twenty-two QOL questions had significant kappa values (0.11 to 0.91). The Cronbach alpha values for 5 domains of QOL ranged from 0.45 to 0.61, indicating that the domains had moderate internal consistency (homogeneity). The initial pool of 38 items was reduced to 27. CONCLUSIONS AND CLINICAL RELEVANCE: The questionnaire was designed to complement veterinary assessment of dogs' physical health. The questionnaire may be a useful tool in making decisions regarding dogs' QOL. 相似文献
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Non‐specific cellular defence mechanisms of rainbow trout (Oncorhynchus mykiss) in intensive and extensive rearing technologies 
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下载免费PDF全文 Elżbieta Terech‐Majewska Patrycja Schulz Edyta Kaczorek Andrzej K Siwicki Józef Szarek Krystyna Skibniewska 《Aquaculture Research》2016,47(11):3585-3592
Water as a habitat is highly variable in terms of the physico‐chemical properties. The aim of the studies was to analyze the quality of the technology by evaluating the non‐specific cellular defence potential of rainbow trout (Oncorhynchus mykiss) in diversified intensity culture systems and in different periods of the breeding cycle. The evaluation was conducted in order to establish the scientific basis for the development of principles for recognition of the culture of this species as ‘organic’. Six rainbow trout rearing farms were selected for the studies and divided into two equal groups according to the production technology: farms with a flow through system (with single water usage – OOH) and facilities with multiple water usage via its backflow (re‐circulating system – RAS). The samples for tests were taken from 20 individuals from each fish farm. In all fish that originated both from OOH‐type farms and RAS operations, an increase in the activity of non‐specific cellular immunity in the autumn was observed. Statistically significant reduction in the activity of cellular defensive mechanisms in small (300–500 g) and big (501–850 g) fish occurred on two 3‐OOH and 3‐RAS farms in spring and autumn in 2011. During these periods, the research has shown the presence of Infectious Pancreatic Necrosis Virus (IPNV), which provoked an immune suppression. Based on the obtained results it is concluded that there were no significant differences in the parameters of non‐specific cellular immunity in rainbow trout originating from different farming systems OOH and RAS. 相似文献
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Edyta Hewelke Jan Szatyłowicz Piotr Hewelke Tomasz Gnatowski Rufat Aghalarov 《Water, air, and soil pollution》2018,229(2):51
The contamination of soil with petroleum products is a major environmental problem. Petroleum products are common soil contaminants as a result of human activities, and they are causing substantial changes in the biological (particularly microbiological) processes, chemical composition, structure and physical properties of soil. The main objective of this study was to assess the impact of soil moisture on CO2 efflux from diesel-contaminated albic podzol soils. Two contamination treatments (3000 and 9000 mg of diesel oil per kg of soil) were prepared for four horizons from two forest study sites with different initial levels of soil water repellency. CO2 emissions were measured using a portable infrared gas analyser (LCpro+, ADC BioScientific, UK) while the soil samples were drying under laboratory conditions (from saturation to air-dry). The assessment of soil water repellency was performed using the water drop penetration time test. An analysis of variance (ANVOA) was conducted for the CO2 efflux data. The obtained results show that CO2 efflux from diesel-contaminated soils is higher than efflux from uncontaminated soils. The initially water-repellent soils were found to have a bigger CO2 efflux. The non-linear relationship between soil moisture content and CO2 efflux only existed for the upper soil horizons, while for deeper soil horizons, the efflux is practically independent of soil moisture content. The contamination of soil by diesel leads to increased soil water repellency. 相似文献
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Edyta Łokas Przemyslaw Wachniew Dariusz Ciszewski Piotr Owczarek Nguyen Dinh Chau 《Water, air, and soil pollution》2010,207(1-4):57-71
This study focuses on the distribution of selected trace metals, 137Cs and 210Pb, in floodplain deposits of the lowland Warta River (southern Poland) downstream of Cz?stochowa, a large city with an iron smelter. The depth profiles of trace metal (Zn, Pb, Cu, Ni, Cd and Mn), 210Pb and 137Cs contents in floodplain sediments were used to derive deposition rates on the floodplain for the twentieth century. The applicability of particular chronometric tools is considered within the context of their mutual relationships and confirmed by the consistency of the results. Deposition rate estimates for the past 50 years based on the vertical patterns of trace metal concentrations, which were correlated with particular events in the development of the smelter, range from 0.4 cm·year?1 in profiles situated in backswamps far from the channel to over 1.1 cm·year?1 in profiles of the natural levee adjacent to the river. Deposition rates based on 210Pb inventories in the profiles range from 0.08 to 0.66 g·cm?2·year?1, which corresponds to linear sedimentation rates of 0.10 to 0.91 cm·year?1, respectively. Dating of characteristic levels associated with peak fallout of 137Cs gives sediment accretion rates resembling those obtained from trace metals and 210Pb. The period of the highest sediment accumulation rate could be related to the highest loads of effluent from the iron smelter and city of Cz?stochowa, which were substantially reduced after the construction of effluent treatment plant. 相似文献
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Edyta Hewelke Jan Szaty&#x;owicz Tomasz Gnatowski Ryszard Oleszczuk 《Land Degradation \u0026amp; Development》2016,27(4):955-964
An understanding of soil moisture content variability is fundamental in hydrological studies of peat soils, whose preservation depend on water‐related processes. Dehydration of fens and adapting them for agricultural production have contributed to the degradation of peat soils. The goal of this study was to determine how the critical soil moisture content (CSMC) and soil water repellency (SWR) affect soil moisture patterns in a degraded peat‐muck soil profile. SWR was measured under laboratory conditions using the water drop penetration time test, and then the CSMC was assessed. An investigation of moisture patterns was based on soil moisture data collected over short distances in a grass‐covered peat‐muck soil profile on seven dates. Observed differences in moisture patterns demonstrate that the CSMC can be used for the prediction of preferential flow occurrences in peat‐muck soils. Lower values of the CSMC and lower levels of SWR persistence in muck layers than in peat layers indicate that degradation of peat soils improves their wettability. The relatively low values of CSMC and the low shrinkage potential in the muck layer suggest that preferential water flow in the degraded organic soils can occur when heavy rains are preceded by long periods of summer drought. Copyright © 2014 John Wiley & Sons, Ltd. 相似文献
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Agnieszka Piernik Katarzyna Hrynkiewicz Anna Wojciechowska Sonia Szymańska Marta Izabela Lis Adele Muscolo 《Archives of Agronomy and Soil Science》2017,63(10):1404-1418
The aim of this study was to determine the influence of selected halotolerant endophytic bacteria isolated from the roots of Salicornia europaea on the growth parameters of Beta vulgaris under different concentrations of salinity. Two endophytic strains were selected as inocula for the pot experiment: Pseudomonas sp. ISE-12 (B1) and Xanthomonadales sp. CSE-34 (B2). Surface-sterilised seeds were incubated in the bacterial inoculation suspensions before sowing and cultivated in a sterile mixture of sand and vermiculite (1:1). Six salinity treatments were taken into account: 0, 50, 100, 150, 200 and 300 mM NaCl. Inoculation of seeds with B1 and B2 positively affected germination percentage and germination index and shortened mean germination time, which led to a quickening of the growth stages of seedlings. After 42 days inoculated plants had, in general, a greater root length, higher dry biomass, lower tissue water content and lower specific leaf area compared with the control. While the positive effect of B2 bacteria was visible only at low salinity, strain B1 stimulated plant growth at higher salinities (200 and 300 mM NaCl). We suggest that the superior growth promotion observed for B1 may be related to the higher metabolic activity of these bacteria. 相似文献
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Dunn ME Taylor SM Shmon CL Wilkinson AA Matte GG Dudzic EM Cantwell S Semple H 《American journal of veterinary research》2002,63(9):1320-1322
OBJECTIVE: To determine tracheal mucociliary clearance rate (TMCCR) by use of a standard protocol in healthy anesthetized cats and to determine the effect of theophylline on TMCCR in healthy anesthetized cats. ANIMALS: 6 healthy cats. PROCEDURE: Cats were anesthetized with propofol, and a droplet of the radiopharmaceutical technetium Tc 99m macroaggregated albumin was placed endoscopically at the carina. Dynamic acquisition scintigraphic imaging was performed, using the larynx as the end point. The TMCCR was determined by measuring the distance the droplet traveled by frame rate. Each cat was imaged 6 times as follows: 3 times following placebo administration and 3 times following the administration of sustained release theophylline (25 mg/kg, PO). Serum theophylline concentrations were assessed during imaging to ensure therapeutic concentrations. RESULTS: The TMCCR in healthy adult cats anesthetized with propofol was 22.2 +/- 2.8 mm/min. Tracheal mucociliary clearance rate in cats receiving theophylline was 21.8 +/- 3.5 mm/min. Theophylline administration did not significantly alterTMCCR. CONCLUSIONS AND CLINICAL RELEVANCE: Theophylline has been shown to increase TMCCR in humans and dogs. In our study, we determined TMCCR in healthy anesthetized cats and found that it was not accelerated by the administration of theophylline. 相似文献