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981.
Katherinne Valderrama Manuel Soto‐Dvila Cristopher Segovia Ignacio Vsquez My Dang Javier Santander 《Journal of fish diseases》2019,42(11):1601-1608
Aeromonas salmonicida subsp. salmonicida (hereafter A. salmonicida) is the aetiological agent of furunculosis in marine and freshwater fish. Once A. salmonicida invade the fish host through skin, gut or gills, it spreads and colonizes the head kidney, liver, spleen and brain. A. salmonicida infects leucocytes and exhibits an extracellular phase in the blood of the host; however, it is unknown whether A. salmonicida have an intraerythrocytic phase. Here, we evaluate whether A. salmonicida infects Atlantic salmon (Salmo salar) erythrocytes in vitro and in vivo. A. salmonicida did not kill primary S. salar erythrocytes, even in the presence of high bacterial loads, but A. salmonicida invaded the S. salar erythrocytes in the absence of evident haemolysis. Naïve Atlantic salmon smolts intraperitoneally infected with A. salmonicida showed bacteraemia 5 days post‐infection and the presence of intraerythrocytic A. salmonicida. Our results reveal a novel intraerythrocytic phase during A. salmonicida infection. 相似文献
982.
983.
Snjeana Pe
ur Kazazi Natalija Topi Popovi Ivan
ica Strunjak‐Perovi Daniela Florio Maria Fioravanti Sanja Babi Rozelindra o‐Rakovac 《Journal of fish diseases》2019,42(8):1201-1209
MALDI‐TOF MS was tested for the identification of Photobacterium damselae subsp. piscicida on isolates grown on two media, cultured at three incubation times and applied on the target plate by the direct sample spotting (DS), by the on‐target extraction (OTE) and by the full extraction (FE) method, in triplicates. The identification of samples grown on blood agar (BA) outperformed identification on tryptic soya agar (TSA) by 0.64% for DS and OTE. The OTE gave the highest scores in both culture media, all incubation times and replicates. Reliable 24‐hr species identification was 61.54%, 84.61% and 53.85% for samples grown on TSA and identified by DS, OTE and FE, respectively. For isolates grown on BA, they were 76.92%, 96.15% and 30.77%, respectively. When identified by OTE, the 48‐hr identification was 93.58%, but for 72 hr declined to 71.79%. The reliable identification with the highest score from the first measurement was 100% only for OTE from BA (24 hr), whereas OTE from TSA gave 84.61% (24 hr), 76.92% (48 hr) and 84.61% (72 hr). The reliable MALDI‐TOF MS identification of Ph. damselae subsp. piscicida is incubation time, media, target plate preparation and replicate‐dependent. 相似文献
984.
985.
Jacquetta Udy Stephen Wing Sorrel O'Connell‐Milne Stina Kolodzey Rebecca McMullin Leonardo Durante Russell Frew 《水产资源保护:海洋与淡水生态系统》2019,29(9):1503-1519
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986.
987.
Marília Hauser Carolina R.C. Doria Roberto V. Santos Aurea García‐Vasquez Marc Pouilly Christophe Pcheyran Emmanuel Ponzevera Gislene Torrente‐Vilara Sylvain Brail Jacques Panfili Audrey Darnaude Jean‐Franois Renno Carmen García‐Dvila Jesus Nuez Franck Ferraton Gladys Vargas Fabrice Duponchelle 《水产资源保护:海洋与淡水生态系统》2019,29(3):397-408
988.
989.
990.