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11.
The authors examined 1,027 persons divided into two groups by means of the toxoplasmin skin test. Group I contained 472 persons who owned domestic cats. Of these 255, i.e. 54.0% gave a positive reaction. Group II consisted of 555 persons who did not own cats. Of these 237, i.e. 42.7% reacted positively. A statistically significant difference at the 1% level of significance was assessed between the two groups. A difference of highest diagnostic importance was found in persons of unexposed occupations in the lowest age group, 14-19 years: 39.5% positive reactions among cat owners and 16.1% among those who did not own cats. The results support the assumption on the role played by domestic cats in the epidemiology of toxoplasmosis. An analysis of the ethology of the cat as regards its importance in the spread of Toxoplasma infection is the subject of the present study. 相似文献
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Lettuce (Lactuca sativa) is the major leafy vegetable that is susceptible to powdery mildew disease under greenhouse and field conditions. Quantitative trait loci (QTLs) for resistance to powdery mildew under greenhouse conditions were mapped in an interspecific population derived from a cross between susceptible L. sativa cultivar Salinas and the highly susceptible L. serriola accession UC96US23. Four significant QTLs were detected on linkage groups LG 1 (pm‐1.1), LG 2 (pm‐2.1 and pm‐2.2) and LG 7 (pm‐7.1), each explaining between 35 to 42% of the phenotypic variation. The four QTLs are not located in the documented hotspots of lettuce resistance genes. Alleles for the disease resistance at the four QTLs originated from both parents (two from each), demonstrating that even highly susceptible accessions may provide alleles for resistance to powdery mildew. These QTLs appeared to operate during limited periods of time. Results of the field trials with F2:3 and F3:4 families derived from a Soraya (moderately resistant) × Salinas cross demonstrated effective transfer of resistance to powdery mildew in this material. An integrated rating approach was used to estimate relative levels of resistance in 80 cultivars and accessions tested in a total of 23 field and greenhouse experiments. Generally, very low resistance was observed in crisphead‐type lettuces, while the highest relative resistance was recorded in leaf and butterhead types. Comparison of two disease assessment methods (percentage rating and the area under the disease progress steps, AUDPS) for detection of QTLs shows that the two approaches complement each other. 相似文献
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Huang Y Olson DJ Gordon JR Middleton DM Simko E 《Canadian journal of veterinary research》2012,76(1):1-7
Absorption of colostral proteins ingested by neonatal piglets within 24 to 36 h after birth is generally considered to be non-selective. Nevertheless, the transfer of colostral proteins, except immunoglubulins, from gut to bloodstream after natural suckling is still poorly characterized. The purpose of this study was to investigate the changes in 2-dimensional electrophoretic plasma protein profiles of neonatal piglets before and after suckling, in order to characterize the gastrointestinal absorption of colostral proteins into the neonatal bloodstream. As expected, the most significant change in plasma after suckling is the presence of a large amount of immunoglobulin. However, while the concentration of a few proteins was mildly increased in post-suckling plasma, the evidence of absorption of colostral non-immunoglobulin proteins by neonatal piglets was not detected in this study. 相似文献
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Simko E Wilcock BP Yager JA 《The Canadian veterinary journal. La revue veterinaire canadienne》2003,44(1):38-42
Apocrine sweat gland adenocarcinomas (AACs) are relatively uncommon skin tumors in dogs. Little prognostic or behavioral information has been published for these tumors. In this retrospective study, 44 AACs from diagnostic archives were reexamined and clinical postexcisional follow-ups for 25 of the 44 cases were obtained by a survey. There were 28 out of 44 (65.9%) AACs that invaded the capsule, stroma, or both, 5 of 44 (11.4%) invaded blood vessels and stroma, and 1 out of 25 (4%) had distant metastases. The presence or absence of stromal and vascular invasion was predicted by clinical examination with more than 80% accuracy. Postexcisional median survival of dogs with AACs was 30 mo at the time of survey. Determination of a correlation between histological features and malignant behavior of AACs was compromised by the low number of cases with clinical AAC-related problems; however, it appears that intravascular invasion is an important indicator of potential systemic metastases. 相似文献
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Farshid Shahriar John R. Gordon Elemir Simko 《Canadian journal of veterinary research》2006,70(4):243-250
Septicemia and endotoxemia initiated by bacterial lipopolysaccharide (LPS) are relatively common in suckling and weaned piglets. Maternal milk is a source of both nutrition and immune protection for piglets. Passive transfer of colostral antibodies is necessary for protection of neonatal piglets against diseases, but the concentration of immunoglobulins in milk rapidly declines during the 1st wk of lactation in all mammals. We hypothesized, therefore, that nonimmunoglobulin substances in milk contribute to the innate protection of neonates against septicemia during the suckling period. Using LPS-affinity chromatography for isolation of LPS-binding proteins and liquid chromatography-mass spectrometry for their identification, we identified in porcine milk the following proteins with LPS-binding capacity: lactoferrin, soluble CD14, serum amyloid A, alpha-S1 casein, beta-casein, and kappa-casein. For lactoferrin, alpha-S1 casein, and kappa-casein, in vitro pepsin digestion did not inhibit LPS-binding activity, whereas combined digestion with pepsin and pancreatin abolished it. The biologic functions of these LPS-binding proteins and peptides were not determined. 相似文献
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S Simko 《Veterinární medicína》1984,29(2):113-119
In flocks with latent infections, 2724 strains of salmonellae (19 serotypes) were isolated from the birds. The following species were represented as follows: S. gallinarum-pullorum (65.08%), S. typhimurium (9.10%), S. bareilly (6.90%), S. enteritidis (2.38%) and S. agona (1.98%). In the foci of salmonelloses 893 strains of salmonellae (19 serotypes) were isolated. The highest representation was found in the S. gallinarum-pullorum (54.20%), S. typhimurium (24.53%), S. bareilly (7.72%), S. choleraesuis (2.29%), S. enteritidis (1.68%), S. infantis (1.54%) and S. anatum (1.23%). Post-mortem examination resulted in recording 15 887 strains of salmonellae (41 serotypes). The following were represented by the largest proportions: S. gallinarum-pullorum (26.62%), S. typhimurium (25.20%), S. bareilly (18.93%), S. infantis (7.20%), S. enteritidis (4.62%), S. agona (3.51%), S. choleraesuis (3.17%), S. anatum (2.19%), S. lille (1.54%) and S. bredeney (1.16%). 相似文献
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Predrag Novakovic Yanyun Y. Huang Betty Lockerbie Farshid Shahriar John Kelly John R. Gordon Dorothy M. Middleton Matthew E. Loewen Beverly A. Kidney Elemir Simko 《Canadian journal of veterinary research》2015,79(2):120-128
F4ac-positive enterotoxigenic Escherichia coli (ETEC) must attach to the intestinal mucosa to cause diarrhea in piglets. Prevention of bacterial attachment to the intestinal mucosa is the most effective defense against ETEC-induced diarrhea. Porcine milk fat globule membranes (MFGM) were shown to be able to inhibit attachment of ETEC to the intestinal brush border; however, the specific components of porcine MFGM that inhibited attachment of ETEC to enterocytes were not identified. Accordingly, the purpose of this study was to identify F4ac-binding MFGM proteins by overlay Western blot and affinity chromatography. The proteome of porcine MFGM was characterized and the following F4ac-binding proteins were detected by overlay Western blot and affinity chromatography: lactadherin, butyrophilin, adipophilin, acyl-CoA synthetase 3, and fatty acid-binding protein 3. The biological function of these proteins was not investigated but it is possible that their interaction with F4ac fimbria interferes with bacterial attachment and colonization. 相似文献
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S Simko 《Veterinární medicína》1984,29(5):287-291
The occurrence of salmonellae in pigs in Slovakia is described for the period from 1971 to 1980. On the whole, 1430 strains (11 serological types) of salmonellae were isolated in stocks with latent infections. The proportions of the serological types were as follows: S. agona 0.69%, S. anatum 0.14%, S. arizona 0.07%, S. bareilly 0.14%, S. decatur 0.07%, S. enteritidis 1.12%, S. give 0.28%, S. heidelberg 0.07%, S. choleraesuis 93.71%, S. panama 0.07% and S. typhimurium 2.45%. In 1315 salmonellosis foci 1333 strains (six serological types) of salmonellae were isolated. The proportions of the serological types were as follows: S. agona 0.37%, S. anatum 0.07%, S. bareilly 0.22%, S. enteritidis 1.20%, S. choleraesuis 90.59% and S. typhimurium 5.30%. The annual pattern of the occurrence of the most frequent serological types is described. 相似文献
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Embury-Hyatt CK Wobeser G Simko E Woodbury MR 《The Canadian veterinary journal. La revue veterinaire canadienne》2005,46(8):702-708
A newly recognized syndrome, characterized by sudden death of farmed deer that are in good to excellent nutritional condition, with lesions of small intestinal mucosal hemorrhage and splenomegaly, is described. Other frequently observed lesions were small intestinal mucosal necrosis, abomasal hemorrhage, random hepatic necrosis, and multifocal hepatic congestion. Clostridium perfringens type A was isolated in high numbers from the intestines of many of the deer affected by the syndrome; however, the numbers were not significantly different from those of control deer. The syndrome did not appear to be related to dietary or any other specific management factors and a definitive cause of the syndrome was not identified. 相似文献