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31.
Universal RAPD primers (OPA02 and OPA07) have been revealed for ladybird beetles of the Coccinellidae family. Using these primers, comparative PCR analysis of ten different ladybird beetle species was conducted. It was shown that Harmonia axyridis beetle species had the highest level of DNA polymorphism and genetic diversity. The RAPD-PCR method for analysis of interspecies differences along with other molecular genetics methods can be used. 相似文献
32.
CCL2 induces neural stem cell proliferation and neuronal differentiation in
Niemann-Pick type C mice
Yu Ri HONG Hyun LEE Min Hee PARK Jong Kil LEE Ju Youn LEE Hwa Deok SUH Min Seock JEONG Jae-Sung BAE Hee Kyung JIN 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(6):693-699
Niemann-Pick type C disease (NP-C) is a rare and ultimately fatal lysosomal storage
disorder with variable neurologic symptoms. Loss of neuronal function and neuronal cell
death occur in the NP-C brain, similar to the findings for other neurodegenerative
diseases. Targeting of neuronal cells in the brain therefore represents a potential
clinical intervention strategy to reduce the rate of disease progression and improve the
quality of life. We previously reported that bone marrow stem cells show a neurogenic
effect through CCL2 (also known as monocyte chemoattractant protein-1, MCP-1) secretion in
the brains of NP-C mice. However, the direct effect of CCL2 on neurogenesis has not been
ascertained. Here, to define neurogenic effects of CCL2 in NP-C, we applied human
recombinant CCL2 to neural stem cells (NSCs) derived from NP-C mice. CCL2-treated NSCs
showed significantly increased capacity for self-renewal, proliferation and neuronal
differentiation. Similar results were observed in the subventricular zone of NP-C mice
after CCL2 treatment. Furthermore, infusion of CCL2 into the NP-C mouse brain resulted in
reduction of neuroinflammation. Taken together, our results demonstrate that CCL2 is a
potential new therapeutic agent for NP-C. 相似文献
33.
Digestible and metabolizable energy in corn grains from different origins for growing pigs 
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下载免费PDF全文 An experiment was conducted to determine the digestible energy (DE) and metabolizable energy (ME) concentrations in nine sources of corn grains fed to growing pigs and to compare the energy values among their countries of origin. A total of nine sources of corn grains including five sources of yellow corn from the United States (USY), two sources of yellow corn from South Africa (SAY), and two sources of white corn from South Africa (SAW) were used. Nine barrows with an initial body weight of 37.1 ± 8.6 kg were allotted to a 9 × 9 Latin square design with nine diets and nine periods. The DE concentration in SAY (3347 kcal/kg) was greater (P < 0.001) than in USY (3269 kcal/kg), but was less (P < 0.001) than in SAW (3436 kcal/kg) on an as‐fed basis. Similarly, the ME concentration in SAY (3291 kcal/kg) was greater (P < 0.001) than in USY (3209 kcal/kg), but was less (P < 0.001) than in SAW (3386 kcal/kg). In conclusion, the DE and ME concentrations in nine sources of corn grains are different among their countries of origin. 相似文献
34.
755 sheep from 41 farms in the districts of Trebisov, Michalovce, Humenné and Roznava in the East Slovakian region were investigated on the presence of antibodies to Bhanja (neutralization test) and tick-borne encephalitis (haemagglutination-inhibition test) viruses. In respective districts 2.2%, 0.4%, 1.7% and 26.7% of animals (total 5.3%) were found to be positive on Bhanja virus, 4.4%, 1.5%, 1.7% and 5.0% (total 2.6%) on tick-borne encephalitis (TBE) virus. The considerable infection rate in sheep with Bhanja virus in the district of Roznava (mean 26.7%-on one farm as high as 63.9% serologically positive animals) can be explained by the local occurrence of the tick Haemaphysalis punctata, the main vector of this virus in Europe. The serologic screening conducted indicates a low activity of natural foci of TBE in eastern Slovakia today, and confirms the existence of a natural focus of infection with Bhanja virus in the district of Roznava. Recent circulation of Bhanja virus is indicated by antibodies detected in several one-year-old sheep. 相似文献
35.
V. I. Kil’ V. Ya. Ismailov I. S. Agas’eva E. N. Besedina E. V. Fedorenko 《Russian Agricultural Sciences》2012,38(3):218-221
The biology and bioregulatory activity of a population of the North American predatory two-spotted stink bug Perillus bioculatus F. are studied and the genetic similarity between this species of predatory bugs and other representatives of the family Pentatomidae is analyzed. It is established that P. bioculatus is of great importance for biological control of the Colorado potato beetle by the natural biocenotic regulation method based on its reproduction under natural conditions. Genetic similarity of the Perillus bug to other representatives of the subfamily Asopinae is shown with the use of RAPD analysis of DNA. 相似文献
36.
J. Kim E.‐J. Kil S. Kim H. Seo H.‐S. Byun J. Park M.‐N. Chung H.‐R. Kwak M.‐K. Kim C.‐S. Kim J.‐W. Yang K.‐Y. Lee H.‐S. Choi S. Lee 《Plant pathology》2015,64(6):1284-1291
Sweet potato leaf curl virus (SPLCV) infects sweet potato and is a member of the family Geminiviridae (genus Begomovirus). SPLCV transmission occurs from plant to plant mostly via vegetative propagation as well as by the insect vector Bemisia tabaci. When sweet potato seeds were planted and cultivated in a whitefly‐free greenhouse, some sweet potato plants started to show SPLCV‐specific symptoms. SPLCV was detected by PCR from all leaves and floral tissues that showed leaf curl disease symptoms. More than 70% of the seeds harvested from SPLCV‐infected sweet potato plants tested positive for SPLCV. SPLCV was also identified from dissected endosperm and embryos. The transmission level of SPLCV from seeds to seedlings was up to 15%. Southern blot hybridization showed SPLCV‐specific single‐ and double‐stranded DNAs in seedlings germinated from SPLCV‐infected seeds. Taken altogether, the results show that SPLCV in plants of the tested sweet potato cultivars can be transmitted via seeds and SPLCV DNA can replicate in developing seedlings. This is the first seed transmission report of SPLCV in sweet potato plants and also, to the authors' knowledge, the first report of seed transmission for any geminivirus. 相似文献
37.
Young Cheol Kim Hyunchae Jung Kil Yong Kim Seur Kee Park 《European journal of plant pathology / European Foundation for Plant Pathology》2008,120(4):373-382
Phytophthora blight of pepper caused by Phytophthora capsici has devastating consequences when combined with other pathogens, including Rhizoctonia solani, Fusarium oxysporum, and Fusarium solani. In order to develop a field-effective biocontrol strategy against Phytophthora blight of pepper, three chitinolytic bacteria,
Serratia
plymuthica strain C-1, strongly antagonistic to P. capsici, Chromobacterium sp. strain C-61, strongly antagonistic to R. solani, and Lysobacter enzymogenes strain C-3, antagonistic to R. solani and Fusarium spp., were selected. In pot studies, application of cultures combining the three bacterial strains effectively suppressed
Phytophthora blight more than application of any single bacterial strain. Bioformulations developed from growth of the strains
in a simple medium containing chitin under large batch conditions resulted in effective control in field applications. Efficacy
of the bioformulated product depended on both the dose and timing of application. Although the undiluted product suppressed
Phytophthora blight under all field conditions, a 10-fold diluted product was effective in solar-sterilized greenhouses and
in fields with crop rotation. These results suggest that the developed product could be a new effective system to control
Phytophthora blight disease in pepper. 相似文献
38.
Sung Dae LEE † Hoi Yun KIM † Young Min SONG Hyun Jung JUNG Sang Yun JI Hae Dong JANG Jae Weon RYU June Cheol PARK Hong Kil MOON In Cheul KIM 《Animal Science Journal》2009,80(1):41-45
The aim of the present study was to investigate the effect of Eucommia ulmoides leaf (EUL) supplementation on the growth performance, blood and meat quality parameters in growing and finishing pigs. Ninety gilts (L × LW × D, 20 kg initialBW) were housed 10 per pen in a front‐open building with three replicate pens per treatment. Experimental treatment was started from the beginning of the growing stage (20 ± 3 kg) by supplementing EUL at 0(C), 3(T1) and 5% (T2) to the growing and finishing diet. Pigs were slaughtered by electrical stunning at 105 ± 3 kg live weight. Average daily feed intake (ADFI, kg/day) decreased (P < 0.05) by addition of EUL in growth performance, average daily gain (ADG, kg/day) was lower (P < 0.05) in T1 and T2 than in C. In hematology, leukocytes (WBC, 103/mm3) decreased (P < 0.05) in T1 and T2 than in C. Erythrocytes (RBC, 106/mm3), hemoglobin (HGB, g/dL) and hematocrit (HCT, %) increased (P < 0.05) in T1 and T2 than in C. Platelet (PLT, 103/mm3) was lower (P < 0.05) in T2 than in C and T1. In biochemical composition of serum, total protein (g/dL), r‐GTP (μ/L), total cholesterol (mg/dL) and triglycerides (mg/dL) were lower (P < 0.05) in T1 and T2 than in C. On longissimus dorsi muscle, crude protein was higher (P < 0.05) in T1 than in C. Crude ash was higher (P < 0.05) in T1 and T2 than in C. Yellow to blue color scale (CIE b*) in meat color was higher (P < 0.05) in T2 than in C. CIE b* in back fat color was higher (P < 0.05) in T2 than in the other treatments. In sensory evaluation scores for fresh meat, the values of meat color, fat color, drip loss and marbling were not significantly affected by addition of EUL. In cooked meat, the values of chewiness and overall acceptability were higher (P < 0.05) in T1 and T2 than in C. The results indicate that the addition of EUL affected growth performance, blood parameters and meat quality parameters in growing and finishing pigs. 相似文献
39.