Pathogenicity assays on Arabidopsis thaliana infected with Xanthomonas campestris pv. campestris seize aggressiveness variation at the race and isolate levels     

Távora  Fabiano T. P. K.  Moura  Dayane R.  Cury  Natália F.  Mendes  Pollyana N.  Quezado-Duval  Alice M.  Mehta  Angela 《European journal of plant pathology / European Foundation for Plant Pathology》2022,164(1):139-145

European Journal of Plant Pathology - Black rot of crucifers caused by Xanthomonas campestris pv. campestris (Xcc) is one of the most destructive diseases affecting vegetable Brassica crops, as...  相似文献   

Gastro-intestinal parasites in calves in colombia     

Bernardo Rivera  Danilo Parra  Obed García  Eduardo Aycardi 《Tropical animal health and production》1983,15(2):107-114

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Ingestive behaviour of steers grazing Brachiaria brizantha cultivar Marandu and in feedlot in Brazil     

de Souza  Sinvaldo Oliveira  Silva  Robério Rodrigues  da Silva  Fabiano Ferreira  de Carvalho  Gleidson Giordano Pinto  da Silva  Ana Paula Gomes  da Silva  João Wilian Dias  Santos  Laize Vieira 《Tropical animal health and production》2019,51(8):2161-2166

Tropical Animal Health and Production - The objective of this study was to evaluate the ingestive behaviour of steers grazing Brachiaria brizantha cv. Marandu and in feedlot regimen in Brazil....  相似文献   

Inclusion of licuri meal in the diet of pasture dairy cows     

Costa  Evely Giovanna Leite  da Silva  Fabiano Ferreira  Silva  Robério Rodrigues  Porto  Antonio Ferraz  Santiago  Bismarck Moreira  Rocha  Leone Campos  Cruz  Aline Gonçalves  Guedes  Anna Caroline Ferreira  Neto  Teotônio Martins  Vieira  Edvaldo Alves 《Tropical animal health and production》2019,51(8):2505-2511

Tropical Animal Health and Production - The objective of this study was to evaluate the inclusion of increasing levels of licuri meal as a substitute for soybean bran in the diet of pasture-based...  相似文献   

Causes of bruising in carcasses of beef cattle during farm,transport, and slaughterhouse handling in Brazil     

Fbio S. Mendona  Ricardo Z. Vaz  Fabiano N. Vaz  Willian S. Leal  Isabella D. B. Silveira  Joo Restle  Arione A. Boligon  Fernando F. Cardoso 《Animal Science Journal》2019,90(2):288-296

This study aimed to assess preslaughter factors responsible for the occurrence of bruises to front, rib, loin, hip, and round sites of cattle carcasses. Data from the slaughter of 148 loads of cattle were assessed, totaling 4,611 carcasses. Evaluated bruising factor variables were animal gender, journey distance (km), vehicle type (m²), journey time (hours), load density (kg/m²), handling and facilities conditions (optimal, acceptable, and unacceptable), and unloading waiting time (min). Gender had the strongest influence on carcass bruising, being the first variable to enter in the majority of models related to the carcass sites. Female carcasses displayed higher bruising in all sites. Densities above 401 kg/m² caused more bruising. With the exception of the loin, unacceptable, and acceptable conditions assigned to farm facilities and handling resulted in higher average bruising. Articulated vehicles with a greater load capacity were more likely to cause bruising. The effects of variables causing bruises act differently in the various carcass sites. In this sense, the results of this study suggested possible management practices for specific problems during the preslaughter period to minimize losses by bruising to different carcass sites.  相似文献   

The use of sulfur hexafluoride microbubbles for contrast‐enhanced ocular ultrasonography of the pecten oculi in birds     

Thiago Alegre Coelho Ferreira  Gabrielle Fornazari  Andr Saldanha  Bruno Lunardeli  Bret A. Moore  Fabiano Montiani‐Ferreira 《Veterinary ophthalmology》2019,22(4):423-429

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Lectin histochemical pattern on the normal and cystic ovaries of sows     

Marcelo HAF Mendes  Maria HB Pinto  Eduardo J Gimeno  Claudio G Barbeito  Fabiano JF de Sant'Ana 《Reproduction in domestic animals》2019,54(10):1366-1374

The lectin histochemical pattern (LHP) was characterized and compared in normal and cystic ovaries of sows. Six biotinylated lectins (PNA, SBA, WGA, RCA‐1, DBA and UEA‐1) were used on tissue sections. In the normal ovaries, the reaction to UEA‐1 and SBA was mild to moderate in mesothelial and endothelial cells. RCA‐1 staining was mild to moderate in theca interna of growing follicles, corpora luteum and mesothelium. In addition, this lectin presented strong reaction in endothelial cells, granulosa cells of atretic follicles, zona pellucida of growing follicles and plasma. DBA showed strong intensity in mesothelial and endothelial cells. There was mild to moderate reactivity to WGA in granulosa cells, corpus luteum and theca interna of follicles in development, and moderate in zona pellucida, in granulosa cells of atretic follicles and mesothelium. PNA staining was mild to moderate in oocytes and in the adventitia and media of medullary arteries. Changes in the LHP of the cystic ovaries were noted; however, there were no differences in these findings between the follicular and luteinized cysts. UEA‐1 reactivity in the cystic ovaries was moderately reduced in the mesothelial and endothelial cells, whereas there was mild reduction in the DBA staining in the granulosa cells. Reaction to RCA‐1 and WGA in the cysts also was decreased in theca interna, zona pellucida and granulosa cells of atretic follicles. Furthermore, endothelium and theca interna in the cystic ovaries presented mild reduction of marcation to SBA, whereas there was decreased reactivity to PNA in the oocytes and adventitia and media layers of the medullary arteries. The results of the current study show that cysts modify the LHP in swine ovaries. These changes of glycoconjugates in many ovarian structures could modify diverse process and may be one of the reasons for decreased fertility in sows.  相似文献   

Development of a qPCR for the detection of infectious laryngotracheitis virus (ILTV) based on the gE gene     

Silvana Santander Parra  Luis Nunez  Marcos R. Buim  Claudete S. Astolfi-Ferreira 《British poultry science》2018,59(4):402-407

1. Infectious laryngotracheitis is a respiratory disease that affects the poultry industry worldwide. It is common in flocks with high-bird density, causing major economic losses.

2. In this study, a SYBR® FAST polymerase chain reaction (PCR) double-strand DNA intercalating agent assay was performed for the detection of infectious laryngotracheitis virus (ILTV) in clinical samples in comparison with a conventional nested-PCR, both based on the glycoprotein E encoding gene. This assay amplified 56 bp and was capable of detecting 1⁹ to 1 copies of virus.

3. In total, 164 clinical samples were obtained from birds with respiratory problems from the period of 2009–2016. In the nested-PCR, there were 45.12% positive samples and 54.88% negative samples, while in the real-time PCR (qPCR), there were 81.1% positive samples and 18.9% negative samples.

4. In conclusion, qPCR from the DNA double-strand intercalating agent SYBR® GREEN FAST was useful for the diagnosis of ILTV because it detected samples that were negative in nested-PCR. This assay has advantages, such as a shortened processing-time, and no need for post-amplification processing (electrophoresis) with additional reagents, such as MgCl₂ and agarose. Hence, qPCR proved to be useful, rapid and low cost for use with clinical samples.  相似文献   

Increasing levels of supplementation for crossbred steers on pasture during the dry period of the year     

Daniele Soares Barroso Neves  Robério Rodrigues Silva  Fabiano Ferreira da Silva  Laize Vieira Santos  George Abreu Filho  Sinvaldo Oliveira de Souza  Marceliana da C. Santos  Weder Jansen Rocha  Ana Paula Gomes da Silva  Mateus de Melo Lisboa  Maria Magna Silva Pereira  Venício Macedo Carvalho 《Tropical animal health and production》2018,50(7):1411-1416

The aim of this study was to evaluate the effect of increasing concentrate supplementation levels on the intake, nutrient digestibility, and performance of crossbred steers during the dry period of the year. The experiment was developed on Princesa do Mateiro farm, in the municipality of Ribeirão do Largo, located in the southwest region of Bahia State, Brazil. Forty uncastrated male crossbred (½ Holstein-Zebu) steers with an average body weight (BW) of 232.55?±?24.97 kg were distributed into four treatments in a completely randomized design with ten replicates. The animals were managed in an experimental area formed by Brachiaria brizantha cv. Marandu, in an intermittent grazing system. Treatments consisted of the following supplementation levels: 0.2% BW, with 60% crude protein (CP); 0.3% BW, with 40% CP; 0.4% BW, with 30% CP; and 0.5% BW, with 24% CP. The intakes of forage dry matter in kg/day and %BW and neutral detergent fiber corrected for ash and protein (NDFap) in %BW decreased linearly, whereas the intake of non-fibrous carbohydrates corrected for ash and protein in kg/day and average daily gain increased linearly. Therefore, the use of supplementation at 0.5% BW (24% crude protein) to provide gains of up to 0.500 kg/day is recommended for grazing steers during the post-weaning period in the dry season of the year.  相似文献   

A morphometric study of the canine skull and periorbita and its implications for regional ocular anesthesia          下载免费PDF全文

Paulo Roberto Klaumann  Juan Carlos Duque Moreno  Fabiano Montiani‐Ferreira 《Veterinary ophthalmology》2018,21(1):19-26

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