Preliminary study of a teaching model for ultrasound-guided peripheral nerve blockade and effects on the learning curve in veterinary anesthesia residents     

Luciano C.B.A. da Silva  Fábio P. Sellera  Ronaldo G. Gargano  Thais C. Rossetto  Giancarlo B. Gomes  Fabiano T. Miyahira  Fábio Futema  Silvia R.G. Cortopassi 《Veterinary anaesthesia and analgesia》2017,44(3):684-687

Objective

To evaluate the use of an experimental colloid model for teaching veterinary anesthesia residents ultrasound-guided technique for nerve blockade.

Study design

Prospective, blinded and randomized.

Methods

Colloid models were constructed for practice in ultrasound-guided needle location. Nine veterinary anesthesia residents with no prior experience of ultrasound-guided technique for nerve blocks were randomly divided into three groups. Each group received theoretical orientation. Two groups were assigned to practical training using the experimental model: group 1 (G1) received 2 hours of training and group 2 (G2) received 1 hour of training prior to testing with specific tasks. Group 3 (G3) received no practical training. During testing, the time required for task completion (e.g., display of structures and positioning a needle) and the number of failures were recorded.

Results

The average times to completion of the tasks and the number of technical failures were: G1, 47 seconds and 1 failure; G2, 68 seconds and 2 failures; G3, 187 seconds and 7 failures.

Conclusions and clinical relevance

In residents with no prior experience of ultrasound-guided needle placement, using an experimental colloid model and a longer training period was associated with increased accuracy and decreased time to task completion. Based on the results of this study, training with an experimental model can be recommended to improve the speed and accuracy of needle manipulation using ultrasound in clinicians with no prior experience of ultrasound-guided technique.  相似文献   

New Insights on the Terpenome of the Red Seaweed Laurencia dendroidea (Florideophyceae,Rhodophyta)     

Louisi Souza de Oliveira  Diogo Antonio Tschoeke  Aline Santos de Oliveira  Lilian Jorge Hill  Wladimir Costa Paradas  Leonardo Tavares Salgado  Cristiane Carneiro Thompson  Renato Crespo Pereira  Fabiano L. Thompson 《Marine drugs》2015,13(2):879-902

The red seaweeds belonging to the genus Laurencia are well known as halogenated secondary metabolites producers, mainly terpenoids and acetogennins. Several of these chemicals exhibit important ecological roles and biotechnological applications. However, knowledge regarding the genes involved in the biosynthesis of these compounds is still very limited. We detected 20 different genes involved in the biosynthesis of terpenoid precursors, and 21 different genes coding for terpene synthases that are responsible for the chemical modifications of the terpenoid precursors, resulting in a high diversity of carbon chemical skeletons. In addition, we demonstrate through molecular and cytochemical approaches the occurrence of the mevalonate pathway involved in the biosynthesis of terpenes in L. dendroidea. This is the first report on terpene synthase genes in seaweeds, enabling further studies on possible heterologous biosynthesis of terpenes from L. dendroidea exhibiting ecological or biotechnological interest.  相似文献   

Thymus vulgaris essential oil and thymol against Alternaria alternata (Fr.) Keissler: effects on growth,viability, early infection and cellular mode of action          下载免费PDF全文

Fabiano J Perina  Douglas C Amaral  Rafael S Fernandes  Claudia RG Labory  Glauco A Teixeira  Eduardo Alves 《Pest management science》2015,71(10):1371-1378

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The Use and Exchange of Biological Control Agents for Food and Agriculture     

J.C.van Lenteren  ;M.J.W.Cock  ;J.Brodeur  ;B.Barratt  ;F.Bigler  ;K.Bolckmans  ;F.Haas  ;P.G.Mason  ;J.R.P.Parra 《中国生物防治》2010,(Z1):123-127

The report sets out to summarize the past and current situation regarding the practice of biologicalcontrol inrelationtothe use and exchange of genetic resources relevant for BCAs.It considers the twomain categories of biological control:classical and augmentative.Allowing access to BCAs for use inanother country imposes no risk of liability to the source country.Local scientific knowledge abouthabitats,fauna andflora,can be helpful  相似文献   

CASE REPORT: Bilateral phacoemulsification in an orangutan (Pongo pygmaeus)     

Fabiano Montiani‐Ferreira  Leandro Lima  Marianna Bacellar  Ricardo G. D’Otaviano Vilani  José Daniel Fedullo  Rogério R. Lange 《Veterinary ophthalmology》2010,13(Z1):91-99

A 14‐year‐old, female, captive‐born orangutan (Pongo pygmaeus) developed bilateral cataracts. Ultrasonography, electroretinography and cataract correction using phacoemulsification were performed bilaterally. This case report aims to describe the ophthalmic procedures performed in this animal critically endangered of extinction. The surgery successfully restored vision and normal activity to the patient.  相似文献   

Localization and genotyping of canine papillomavirus in canine inverted papillomas     

Margherita Orlandi  Maurizio Mazzei  Marta Vascellari  Erica Melchiotti  Claudia Zanardello  Ranieri Verin  Francesco Albanese  Fabiano Necci  Luca Pazzini  Giulia Lazzarini  Francesca Abramo 《Journal of veterinary diagnostic investigation》2021,33(6):1069

Numerous canine papillomaviruses (CPVs) have been identified (CPV1–23). CPV1, 2, and 6 have been associated with inverted papillomas (IPs). We retrieved 19 IPs from 3 histopathology archives, and evaluated and scored koilocytes, inclusion bodies, giant keratohyalin granules, cytoplasmic pallor, ballooning degeneration, and parakeratosis. IHC targeting major capsid proteins of PV was performed, and CPV genotyping was achieved by PCR testing. Tissue localization of CPV DNA and RNA was studied by chromogenic and RNAscope in situ hybridization (DNA-CISH, RNA-ISH, respectively). IPs were localized to the limbs (50%), trunk (30%), and head (20%), mainly as single nodules (16 of 19). In 15 of 19 cases, immunopositivity was detected within the nuclei in corneal and subcorneal epidermal layers. PCR revealed CPV1 in 11 IPs and CPV2 DNA in 3 IPs. Overall, 14 of 17 cases were positive by both DNA-CISH and RNA-ISH, in accord with PCR results. A histologic score >5 was always obtained in cases in which the viral etiology was demonstrated by IHC, DNA-CISH, and RNA-ISH. IHC and molecular approaches were useful to ascertain the viral etiology of IPs. Although IHC is the first choice for diagnostic purposes, ISH testing allows identification of PV type and the infection phase. RNA-ISH seems a promising tool to deepen our understanding of the pathogenesis of different PV types in animal species.  相似文献   

Impact of new measured Mediterranean mineralization rates on the fate of simulated aquaculture wastes     

Patrizia De Gaetano  Paolo Vassallo  Marco Bartoli  Daniele Nizzoli  Andrea M. Doglioli  Marcello G. Magaldi  Mauro Fabiano 《Aquaculture Research》2011,42(9):1359-1370

In order to provide values of key parameters in aquaculture waste degradation modelling specifically for the Mediterranean, sampling campaigns were carried out in 2006. Accurate measurements of particulate carbon input and benthic respiration rates were performed using sediment traps and intact core incubations. The in situ measurements, compared with data from Atlantic salmon production, showed lower carbon flux and oxygen consumption, while a greater degradation capability was observed. Moreover, a temperature dependence of the benthic parameters was highlighted. Successively, the model Finite Organic Accumulation Module was used for an accurate comparison between different parameterizations. Finite Organic Accumulation Module simulates the organic carbon degradation and the net carbon accumulation on the sediment, yielding a benthic state index. In comparison with previous results, there was a decrease in benthic impacts due to minor inputs of carbon and higher mineralization rates. Moreover, a seasonal variation could be observed in the organic carbon concentration. Nevertheless, the new results remain consistent with the old ones on two points: (a) the negligible benthic impact of faeces with respect to uneaten feed and (b) the dependence of that impact on the different feed release conditions.  相似文献   

Pacific approaches to fundraising in the digital age: COVID-19, resilience and community relational economic practices     

Lorena de la Torre Parra  Apisalome Movono  Regina Scheyvens  Sophie Auckram 《Asia Pacific viewpoint》2023,64(2):222-238

The aim of this paper is to discuss how community relational economic practices in virtual spaces are effective in building resilience because they are borne of and sustained by familiar traditional Fijian values of collective work and social interdependence. The researchers adopted a pandemic-induced methodology, conducting online-based talanoa (fluid conversations between two or more people) with a number of people leading, or involved in, these initiatives. We also engaged with online community groups behind a number of initiatives. Examples are provided of online crowdfunding, livestreaming of concerts to solicit donations, and bartering facilitated by social media sites. To conclude, we stress the enduring nature of communal bonds and traditional systems which Pacific people readily adapt and translate into different forums and forms in the face of challenges such as the restrictions and financial hardships caused by COVID-19. The findings highlight that solesolevaki – a tradition of working together for a common cause – can also occur in the digital era: this demonstrates the deep connection of Fijian peoples and their sense of obligation to one another and to their culture, regardless of where they are in the world.  相似文献   

Gram-negative bacterial diversity and evidence of international clones of multidrug-resistant strains in zoo animals     

João Pedro Rueda FURLAN  Micaela Santana RAMOS  Fábio Parra SELLERA  Irys Hany Lima GONZALEZ  Patrícia Locosque RAMOS  Eliana Guedes STEHLING 《Integrative zoology》2024,19(3):417-423

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Genetic diversity and structure of wild and managed populations of Polaskia chende (Cactaceae) in the Tehuacán-Cuicatlán Valley,Central Mexico: insights from SSR and allozyme markers     

Gonzalo Contreras-Negrete  M. Eva Ruíz-Durán  Dánae Cabrera-Toledo  Alejandro Casas  Ofelia Vargas  Fabiola Parra 《Genetic Resources and Crop Evolution》2015,62(1):85-101

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