Methods of microbial control in marine fish larval rearing: clay‐based turbidity and passive larval transfer          下载免费PDF全文

Kevin Stuart  Federico Rotman  Mark Drawbridge 《Aquaculture Research》2016,47(8):2470-2480

This study focused on methods to reduce bacterial loads in the larval culture tanks of California yellowtail (Seriola lalandi). We conducted two trials to evaluate methods to minimize bacterial loads in the larval rearing water. The first trial examined the use of bentonite clay as a turbidity agent to replace algae in a green water‐type environment. This trial consisted of three treatments: (1) clay with continuous feeding (CCO), (2) clay with batch feedings (CBA) and (3) algae paste with batch feedings (ALG). The results showed that both clay treatments had significantly fewer Vibrio colonies in the water column (CBA – 180 ± 78; CCO – 377 ± 120 CFU mL^?1) than the ALG treatment (5692 ± 2396 CFU mL^?1) after 14 days of culture. Survival was significantly higher in the CCO treatment (14.1 ± 2.6%) than either the CBA (2.3 ± 0.5%) or ALG treatments (2.8 ± 1.5%). The second trial attempted to limit bacterial loading in the larval culture tank by passively transferring the larvae into an adjacent, clean tank at 1, 5 and 9 days post hatch during the first 2 weeks of culture. The results from this trial showed that after 12 days of culture, water in the transfertank had fewer Vibrio colonies (1025 ± 541 CFU mL^?1) than the water in the control tanks (1962 ± 1415 CFU mL^?1). Also, survival was significantly higher among larvae that were transferred (43.9 ± 13.5%) than in the control tanks (23.1 ± 6.3%).  相似文献   

Assessing the agronomic potential of linseed genotypes by multivariate analyses and association mapping of agronomic traits     

Braulio?J.?Soto-Cerda  Fernando?Westermeyer  Federico?I?iguez-Luy  Gastón?Mu?oz  Adolfo?Montenegro  Sylvie?CloutierEmail author 《Euphytica》2014,196(1):35-49

High prices of fish oil make linseed attractive for aquaculture and animal feed. To ensure a constant supply of linseed, the development of stable cultivars is of strategic importance. In this study, 35 linseed genotypes were evaluated in five Chilean environments (E) from 2009 to 2012. The additive main effect and multiplicative interaction analysis (AMMI), genotype (G) plus genotype by environment (GE) interaction (GGE) biplot analysis and three stability parameters were tested with the aim of identifying adapted genotypes for the development of linseed cultivars. An association mapping (AM) analysis was also conducted for four agronomic traits and the stability of the associated markers was evaluated using the QQE (QTL main effect and QTL by environment interaction) approach. Combined analysis of variance for yield, seeds per boll (SPB), plant height (PH) and days to flowering (DTF) were significant for G, E and GE (P < 0.001). The combined stability analysis identified some Canadian, Argentinean and Chilean accessions to be the best adapted and highest yielding genotypes. Coancestry analysis indicated that crossing Canadian and Chilean genotypes could maximize transgressive segregation for yield. Significant associations for DTF, PH and SPB explained up to 59 % of the phenotypic variation for these traits. The QQE and AM analyses were consistent in identifying marker LGM27B as the most stable and significant across all environments with the largest effect in reducing DTF. The combined application of the stability, AM and QQE analyses could accelerate the development of marketable linseed cultivars adapted to Southern Chile.  相似文献   

Differential response of male and female Diabrotica speciosa (Coleoptera: Chrysomelidae) to bitter cucurbit-based toxic baits in relation to the treated area size     

G. Cabrera Walsh  Federico Mattioli  Donald C. Weber 《国际虫害防治杂志》2014,60(2):128-135

Cucurbitacin-rich extracts are phagostimulants for both male and female Luperini, but field trials reveal that males are far more attracted to them than females. The sex ratio and number of beetles killed by an application of cucurbitacin-based toxic baits was assessed at two different scales: small areas of 100 m², and a larger area of 10,000 m². The small area bait applications killed mostly males (95.2%), but the 1-ha treated plot showed a lower sexual bias (57% male). An earlier study indicated point sources (sticky traps) attracted only males. Results indicate that the distance attraction to bitter cucurbit extracts is almost exclusive to males, but the wide dispersal of a toxic bait based on them should promote encounter and control of both sexes within the treated area. No significant non-target effects were observed.  相似文献   

A wind-oriented sticky trap for evaluating the behavioural response of the leaf-beetle Diabrotica speciosa to cucurbit extracts     

G. Cabrera Walsh  Federico Mattioli  Donald C. Weber 《国际虫害防治杂志》2014,60(1):46-51

Cucurbitacins are plant defensive chemicals that attract many species of adapted insect herbivores such as luperine leaf beetles, and they have been employed in traps and toxic baits. Male and female beetles feed avidly on baits containing these compounds, but field trials have revealed that males find them far more attractive than females. A wind-oriented baited sticky trap was designed to evaluate the response of Diabrotica speciosa to cucurbit extracts, by means of a mark–release–recapture experiment. The spatial range of attraction was less than 20 m, and captures were almost exclusively of males (99.91%). Distance attraction for D. speciosa to a point-source of bitter cucurbit juices is probably exclusive to males. These conclusions probably apply to most Diabrotica species, of which many are crop pests.  相似文献   

PCV2-DNA in formalin-fixed and paraffin embedded lymph nodes of wild boar (Sus scrofa ssp. scrofa): one sampling approach for two laboratory techniques     

Federico Morandi  Serena Panarese  Ranieri Verin  Fabio Ostanello  Cinzia Benazzi  Giuseppe Sarli 《Acta veterinaria Scandinavica》2012,54(1):17

Superficial inguinal lymph nodes from 72 wild boars examined in a previous immunohistochemical (IHC) study on porcine circovirus type 2 (PCV2) were selected for a PCV2 polymerase chain reaction (PCR) analysis. Four of these lymph nodes were PCV2-IHC strongly positive with PMWS histological lesions (outcome 1), 6 weak to mild PCV2-IHC positive without PMWS histological lesions (outcome 2) and 62 PCV2-IHC negative. Considering IHC the gold standard for diagnosis, the aims of the study were to evaluate the suitability of the PCV2-DNA extraction from formalin-fixed and paraffin-embedded (FFPE) tissue and the sensitivity and specificity of PCR under two IHC interpretations criteria: (A) the sample was considered positive if the result was outcome 1; (B) the sample was considered positive if the result was outcome 1 or 2. Under (A) criteria, sensitivity and specificity of PCR were 100% and 89.7%, respectively; the Cohen''s Kappa coefficient was 0.49. Under (B) criteria, sensitivity and specificity of PCR were 80.0% and 95.2%, respectively; the Cohen''s Kappa coefficient was 0.72. The high Cohen''s Kappa coefficient under the (B) interpretative criteria indicates good agreement between the two methods. In conclusion, 1) DNA extracted from FFPE specimens of wild boar is suitable for PCR and further represents a screening test for PCV2/PCVD (PCV2 Diseases) investigations in wild boar as well; 2) routine histological sampling can also be useful for PCV2 virological studies in wild boar.  相似文献   

Multiple attribute decision making for individual tree detection using high-resolution laser scanning     

Giovanni Forzieri  Leonardo Guarnieri  Enrique R. Vivoni  Fabio Castelli  Federico Preti 《Forest Ecology and Management》2009,258(11):2501-2510

A canopy height model (CHM) is a standard LiDAR-derived product for deriving relevant forest inventory information, including individual tree positions, crown boundaries and plant density. Several image-processing techniques for individual tree detection from LiDAR data have been extensively described in literature. Such methods show significant performance variability depending on the vegetation characteristics of the monitored forest. Moreover, over regions of high vegetation density, existing algorithms for individual tree detection do not perform well for overlapping crowns and multi-layered forests. This study presents a new time and cost-efficient procedure to automatically detect the best combination of the morphological analysis for reproducing the monitored forest by estimating tree positions, crown boundaries and plant density from LiDAR data. The method needs an initial calibration phase based on multi attribute decision making-simple additive weighting (MADM-SAW). The model is tested over three different vegetation patterns: two riparian ecosystems and a small watershed with sparse vegetation. The proposed approach allows exploring the dependences between CHM filtering and segmentation procedures and vegetation patterns. The MADM architecture is able to self calibrate, automatically finding the most accurate de-noising and segmentation processes over any forest type. The results show that the model performances are strongly related to the vegetation characteristics. Good results are achieved over areas with a ratio between the average plant spacing and the average crown diameter (TCI) greater than 0.59, and plant spacing larger than the remote sensing data spatial resolution. The proposed algorithm is thus shown a cost effective tool for forest monitoring using LiDAR data that is able to detect canopy parameters in complex broadleaves forests with high vegetation density and overlapping crowns and with consequent significant reduction of the field surveys, limiting them over only the calibration site.  相似文献   

Technology generation to dissemination: lessons learned from the tef improvement project     

Gina Cannarozzi  Solomon Chanyalew  Kebebew Assefa  Abate Bekele  Regula Blösch  Annett Weichert  Dominik Klauser  Sonia Plaza-Wüthrich  Korinna Esfeld  Moritz Jöst  Abiel Rindisbacher  Habte Jifar  Victoria Johnson-Chadwick  Ermias Abate  Wuyan Wang  Rizqah Kamies  Negussu Husein  Worku Kebede  Kidist Tolosa  Yazachew Genet  Kidu Gebremeskel  Brikti Ferede  Firew Mekbib  Federico Martinelli  Hans Christian Pedersen  Suhail Rafudeen  Shimelis Hussein  Muluneh Tamiru  Naomi Nakayama  Mike Robinson  Ian Barker  Samuel Zeeman  Zerihun Tadele 《Euphytica》2018,214(2):31

Indigenous crops also known as orphan crops are key contributors to food security, which is becoming increasingly vulnerable with the current trend of population growth and climate change. They have the major advantage that they fit well into the general socio-economic and ecological context of developing world agriculture. However, most indigenous crops did not benefit from the Green Revolution, which dramatically increased the yield of major crops such as wheat and rice. Here, we describe the Tef Improvement Project, which employs both conventional- and molecular-breeding techniques to improve tef—an orphan crop important to the food security in the Horn of Africa, a region of the world with recurring devastating famines. We have established an efficient pipeline to bring improved tef lines from the laboratory to the farmers of Ethiopia. Of critical importance to the long-term success of this project is the cooperation among participants in Ethiopia and Switzerland, including donors, policy makers, research institutions, and farmers. Together, European and African scientists have developed a pipeline using breeding and genomic tools to improve the orphan crop tef and bring new cultivars to the farmers in Ethiopia. We highlight a new variety, Tesfa, developed in this pipeline and possessing a novel and desirable combination of traits. Tesfa’s recent approval for release illustrates the success of the project and marks a milestone as it is the first variety (of many in the pipeline) to be released.  相似文献   

Cytology of the healthy canine and feline ocular surface: comparison between cytobrush and impression technique          下载免费PDF全文

Anna Perazzi  Federico Bonsembiante  Maria Elena Gelain  Marco Patruno  Enzo Di Iorio  Angelo Migliorati  Ilaria Iacopetti 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2017,46(1):164-171

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A tree species range in the face of climate change: cork oak as a study case for the Mediterranean biome     

Federico Vessella  Javier López-Tirado  Marco Cosimo Simeone  Bartolomeo Schirone  Pablo J. Hidalgo 《European Journal of Forest Research》2017,136(3):555-569

Species distribution models are feasible methods for projecting theoretical responses of living organisms’ occurrence under several future climate change scenarios. The major interest is focused on trees, which regulate the equilibrium within ecosystems and guarantee the survival of many life forms on the Earth. The repercussions of climatic drivers are expected to pose the strongest threats for the Mediterranean biome, an acknowledged hotspot of biodiversity. Here, we focused on cork oak (Quercus suber L.), a keystone species of many landscapes, sustaining a rich biodiversity, ecological processes and economic incomes. Results of 8 combined ecological modelling techniques and two Global Circulation Models highlight a broad contraction of the species potential range over the twenty-first century, both under intermediate and high emissions scenarios. Coupled northward and upward shifts are predicted, mostly pertaining Iberia and North Africa. The potential areas detected at Levantine will likely undergo disappearance. To exacerbate the impacts of climate change, the future of the ecosystems linked to cork oak remains uncertain, because of the expected implications on the phenotypic plasticity or evolutionary responses. A synergy among niche-based, physiological and eco-genetic investigations is strongly needed in the field of applied research, to improve the assessment of conservation and reforestation actions.  相似文献   

Characterisation of the phospholipid fraction of hulled and naked tetraploid and hexaploid wheats     

Marco Pelillo  Federico Ferioli  Giovanna Iafelice  Emanuele Marconi  Maria Fiorenza Caboni 《Journal of Cereal Science》2010

Phospholipid (PL) profile was determined on lipids extracted from the whole grains of 10 genotypes of tetraploid wheats (Triticum dicoccon Schrank and Triticum durum Desf.) and 10 genotypes of hexaploid wheats (Triticum spelta L. and Triticum aestivum L.) by a liquid chromatography system coupled on-line with an evaporative light scattering detector (HPLC-ELSD). Phosphatidylethanolamine, phosphatidylinositol and phosphatidylcholine were the major PL determined, accounting for more than 70% of total PL. PL represented about 10% of total lipids and were detected in naked wheats at levels of 10.1 and 10.4 g/100 g of lipids (T. durum and T. aestivum, respectively) whereas in the corresponding ancestors T. dicoccon and T. spelta PL amounts were 8.4 and 9.1 g/100 g of lipids, respectively. PL amount in naked wheats was about 17% higher than that determined on average in hulled wheats: 10.2 ± 2.8 vs. 8.7 ± 2.2 g/100 g of lipids, respectively. A hyphenated system HPLC-ELSC was revealed as a useful and more informative tool to identify different PL classes with a minimal experimental workout in comparison to the organic phosphorus assay. The identification of PL molecular species was performed by HPLC, employing as detector a mass spectrometer working in negative ASI-ES mode.  相似文献