Bioactivity of extracts and isolated compounds from Vitex polygama (Verbenaceae) and Siphoneugena densiflora (Myrtaceae) against Spodoptera frugiperda (Lepidoptera: Noctuidae)     

Gallo MB  Rocha WC  da Cunha US  Diogo FA  da Silva FC  Vieira PC  Vendramim JD  Fernandes JB  da Silva MF  Batista-Pereira LG 《Pest management science》2006,62(11):1072-1081

The effects of crude extracts, fractions and isolated compounds from Vitex polygama Cham. and Siphoneugena densiflora Berg were evaluated on the development of Spodoptera frugiperda JE Smith, a destructive insect pest of corn and several other crops. The extracts and fractions were incorporated into an artificial diet at 1 mg g(-1) and offered to the insect during its larval stage. Length and viability of larval and pupal stages as well as pupal weight were assessed. Isolated compounds were tested through superficial contamination of the diet at 0.1 mg g(-1). Weight and viability of ten-day-old larvae were determined. Methanolic and hydroalcoholic S. densiflora extracts caused 100% larval mortality, while leaf and fruit hydroalcoholic extracts from V. polygama were the most active. Among the isolated compounds, flavonoids presented the best insecticidal results, and tannins the best larval growth inhibition.  相似文献   

Molluscicidal and cercaricidal activities of curcumin on Biomphalaria glabrata and Schistosoma mansoni cercariae     

Jaqueline L Matos  Karen R da Silva  Lucas A de Lima Paula  Wilson R Cunha  Salvador B Ramos  Vanderlei Rodrigues  Fernanda J Cabral  Lizandra G Magalhes 《Pest management science》2020,76(4):1228-1234

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Effect of yeast products supplementation during transition period on metabolic profile and milk production in dairy cows     

Faccio-Demarco  Claudia  Mumbach  Tatiele  Oliveira-de-Freitas  Vanessa  Fraga e Silva-Raimondo  Raquel  Medeiros-Gonçalves  Fernanda  Nunes-Corrêa  Marcio  Burkert-Del Pino  Francisco Augusto  Mendonça-Nunes-Ribeiro Filho  Henrique  Cassal-Brauner  Cássio 《Tropical animal health and production》2019,51(8):2193-2201

Tropical Animal Health and Production - The aim of the current study was to assess the effect that supplementation with yeast culture plus enzymatically hydrolyzed yeast (YC-EHY) during the...  相似文献   

Supplementation with Ca salts of soybean oil interacts with concentrate level in grazing dairy cows: intake,ingestive behavior,and ruminal parameters     

Fernanda Lopes Macedo  Fernanda Batistel  Jonas de Souza  Lucas Jado Chagas  Flávio Augusto Portela Santos 《Tropical animal health and production》2016,48(8):1593-1598

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Electrocardiographic parameters of captive lions (Panthera leo) and tigers (Panthera tigris) immobilized with ketamine plus xylazine     

Maria Helena M A Larsson  Fernanda M Coelho  Valéria M C Oliveira  Fernanda L Yamaki  Guilherme G Pereira  Elaine C Soares  José Daniel L Fedullo  Roberto C Pereira  F H Ito 《Journal of zoo and wildlife medicine》2008,39(3):314-319

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Effect of human tubal fluid medium and hyperactivation inducers on stallion sperm capacitation and hyperactivation     

Camila Arroyo‐Salvo  Francisco Sanhueza  Fernanda Fuentes  Favin Treuln  María Elena Arias  Paulina Cabrera  Mauricio Silva  Ricardo Felmer 《Reproduction in domestic animals》2019,54(2):184-194

Conventional in vitro fertilization has not yet been implemented in the equine species. One of the main reasons has been the inability to develop a culture medium and incubation conditions supporting high levels of stallion sperm capacitation and hyperactivation in vitro. Although different culture media have been used for this purpose, human tubal fluid (HTF) medium, widely used in the manipulation of human and mice gametes, has not been reported so far in stallion sperm culture. The first part of this study aimed to compare HTF and Whitten's media on different stallion sperm quality and capacitation variables. Additionally, the effect of procaine, aminopyridine and caffeine in both media was evaluated on sperm motility parameters at different incubation times. Integrity and destabilization of the plasma membrane were evaluated by merocyanine 540/SYTOX Green (MC540), mitochondrial membrane potential (?Ψm) using tetramethylrhodamine methyl ester perchlorate (TMRM), acrosome membrane integrity by PNA/FITC and tyrosine phosphorylation by P‐tyrosine mouse mAb conjugated to Alexa Fluor® by flow cytometry. Motility parameters were evaluated using the integrated semen analysis system (ISAS®). We found no differences between Whitten's and HTF media and incubation time in terms of sperm viability, uninduced acrosome membrane damage or mitochondrial membrane potential at 30‐ and 120‐min incubation. Membrane fluidity (MC540) increased in both media at 30‐ and 120‐min incubation compared to noncapacitating conditions. Similarly, tyrosine phosphorylation increased in both media in capacitating conditions at 2‐ and 4‐hr incubation compared to noncapacitating conditions. Although procaine showed the best result in terms of sperm hyperactivated motility in both media, aminopyridine also showed parameters consistent with the hyperactivation including an increase in curvilinear velocity and decrease in straightness. In conclusion, HTF medium and aminopyridine equally support capacitation‐related parameters in stallion sperm.  相似文献   

Low occurrence of pathogenic Yersinia enterocolitica in pig tonsils at slaughter in Southern Brazil     

Wildemann  Paula  Gava  Danielle  Sfaciotte  Ricardo Antônio Pilegi  Melo  Fernanda Danielle  Ferraz  Sandra Maria  da Costa  Ubirajara Maciel  Vaz  Eliana Knackfuss 《Tropical animal health and production》2018,50(3):671-675

Tropical Animal Health and Production - Yersinia enterocolitica is a foodborne pathogen and pigs are the main reservoir of it in their tonsils. As Brazil is a large producer and exporter of pork...  相似文献   

Antibiotic susceptibility of <Emphasis Type="Italic">Staphylococcus aureus</Emphasis> isolated from subclinical bovine mastitis cases and in vitro efficacy of bacteriophage     

Diego F. Varela-Ortiz  José E. Barboza-Corona  Joaquín González-Marrero  Ma. Fabiola León-Galván  Mauricio Valencia-Posadas  Alma A. Lechuga-Arana  Cynthia G. Sánchez-Felipe  Fernanda Ledezma-García  Abner J. Gutiérrez-Chávez 《Veterinary research communications》2018,42(3):243-250

Staphylococcus aureus is an opportunistic pathogen that may cause severe infections in livestock, and represents the major cause of mastitis in dairy cows. Currently, instead of using antibiotics, new strategies are sought to reduce this clinical health problem. The aim of this study was to determine the efficacy of phage therapy to kill S. aureus strains obtained from farms located at the State of Guanajuato, México. Thirty-six S. aureus strains from cow milk with subclinical mastitis were isolated and identified, and the susceptibility to antibiotics and four phages also isolated in this work was tested. It was found that more of 90% of S. aureus isolates were not susceptible to six or more antibiotics, and 100% were resistant to penicillin, dicloxacillin, cefotaxime, ampicillin and cephalothin, and 81 and 77%, to tetracycline and cefuroxime, respectively. Fortunately, 100% of S. aureus isolates were susceptible to phages used in this work, which was detected as clear zones using specific phage. It was shown for the first time, that phages used in this study are active against pathogenic S. aureus and might be incorporated into the therapy as an important tool for the control of staphylococcal bovine mastitis, specially to antibiotic-resistant S. aureus strains isolated in farm located at the state of Guanajuato, México; and its use might be extended to other regions inside or outside the country.  相似文献   

Unraveling avian and reptilian hematology: An optical and electron microscopic study of the buffy coat          下载免费PDF全文

Filipe Fontes Pinto  Célia Lopes  Fernanda Malhão  Ricardo Marcos 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2018,47(3):407-414

Background

Blood centrifugation and buffy coats are at the cornerstone of hematology. In mammals, the buffy coat has a layered disposition (from bottom to top) with neutrophils on top of erythrocytes, followed by monocytes/lymphocytes, and platelets. In nonmammals, this distribution is unknown. Recently, the cell tube block (CTB) technique was developed to study the buffy coat, but it was never applied to nonmammal buffy coats.

Objectives

This study aimed to evaluate using the CTB technique to study reptilian and avian buffy coats and to propose its use for clinical applications.

Methods

Blood from five birds and eight reptiles of different species was obtained to make CTBs that were processed for optical/electron microscopy. H&E, Sirius red, and immunohistochemistry staining against CD3 (to label T lymphocytes) were applied to the CTBs.

Results

In birds, the buffy coat had a layered appearance with the granulocyte layer containing granulocytes (heterophils and eosinophils) and nucleated erythrocytes followed by a mononuclear cell layer containing lymphocytes, monocytes, and thrombocytes. In some animals, a nucleated erythrocyte layer was observed admixed with the granulocyte/mononuclear cell layer. A small clot within the buffy coat was seen in seven reptiles, and less definition of layers occurred in reptiles, with only one or two layers. Lymphocytes appeared toward the top of the buffy coat.

Conclusions

From a comparative hematology perspective, the buffy coat of mammals differs from that of birds and more from that of reptiles. The CTB technique can be used to study these differences in avian and reptilian hematology, especially to study atypical circulating cells, hemoparasites, or blood cell proportions in health and disease.  相似文献   

Phosphorus Budget in Integrated Multitrophic Aquaculture Systems with Nile Tilapia,Oreochromis niloticus,and Amazon River Prawn,Macrobrachium amazonicum          下载免费PDF全文

Fernanda S. David  Danilo C. Proença  Wagner C. Valenti 《Journal of the World Aquaculture Society》2017,48(3):402-414

Integrated multitrophic aquaculture (IMTA) systems are designed mainly for efficient use of resources. Substrates added to aquaculture ponds provide space for periphyton to settle and recover nutrients, making these nutrients available to the species being reared. The present study is centered on the phosphorus budget, analyzing the main ecological compartments of IMTA systems in earthen ponds stocked with Amazon River prawn, Macrobrachium amazonicum, and Nile tilapia, Oreochromis niloticus, with or without different added substrates. The experimental design was completely randomized, with three treatments (without a substrate, with a geotextile fabric substrate, and with a bamboo substrate) and four replications. Phosphorus entered the systems mainly in tilapia feed (ca. 50–61%), inlet water (ca. 17–27%), and fertilizer (ca. 6–7%). Input of phosphorus from other compartments ranged from 1.5 to 1.9%. Most phosphorus was accumulated at the pond bottom as sediment (ca. 60–68%) and fish biomass (ca. 18–26%), or discharged in the outlet water (ca. 7–10%). Feeding is the main driver for the distribution of phosphorus in the ponds. Levels of phosphorus retained in reared animals (20–28%) were higher in these IMTA systems than in tilapia and prawn monocultures (reported as 10–20% and 10–13%, respectively). Nonetheless, the present data showed that the addition of different types of substrates might not improve the recovery of phosphorus in animal biomass as initially supposed. Even so, these IMTA systems decreased the amount of phosphorus released in effluents, and this decrease was enhanced by the addition of substrates, reducing the impact on the receiving waterbodies.  相似文献