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661.
Bussadori C Pradelli D Borgarelli M Chiavegato D D'Agnolo G Menegazzo L Migliorini F Santilli R Zani A Quintavalla C 《Veterinary journal (London, England : 1997)》2009,182(2):187-192
This prospective study was conducted to evaluate the prevalence and severity of ocular lesions in dogs with anemia (packed cell volume 20%) and/or thrombocytopenia (platelet count < 150,000/μL). Dogs were divided into four groups: (1) anemic (n = 17); (2) thrombocytopenic (n = 36); (3) anemic and thrombocytopenic (n = 24); and (4) healthy controls (n = 26). The prevalence of ophthalmic lesions in these four groups was 12%, 42%, 42% and 0%, respectively. Anemia was not associated with the presence of ocular lesions (P = 0.41) or their severity. Thrombocytopenia was significantly associated with the presence of ocular lesions (P = 0.0016) and with their severity (P = 0.017). We conclude that thrombocytopenia, but not anemia, is associated with ocular lesions in dogs. 相似文献
662.
Scaramozzino P Di Cave D Berrilli F D'Orazi C Spaziani A Mazzanti S Scholl F De Liberato C 《Veterinary journal (London, England : 1997)》2009,182(2):231-234
Giardia duodenalis is a protozoan parasite of animals that is zoonotic. Given the capacity of this organism to spread via the faecal–oral route, animals held in overcrowded and unhygienic conditions are at high risk of infection. Faecal samples from dogs in three kennels in Rome were examined by microscopy and PCR for G. duodenalis, and the prevalence data generated were correlated with variables such as kennel identity, age of dog, length of time the dog had been kennelled and clinical signs.The overall prevalence of the parasite in the faecal samples was 20.5% and was higher in samples from the largest kennel, which had the greatest turnover of dogs, and in faecal samples from younger animals. Giardia cysts were found more frequently in diarrhoeic animals but were also found in dogs with no clinical signs. Although the finding that the majority of isolates were dog-specific rather than zoonotic genotypes suggests that the zoonotic risk from this pathogen is less than previously thought, the higher prevalence of infection in younger dogs may pose a specific public health issue as such animals are more frequently re-homed with families. 相似文献
663.
Barbara Lamagna Orlando Paciello† Manuela Ragozzino Serenella Papparella† Serena Montagnaro‡ Francesco Lamagna 《Veterinary ophthalmology》2009,12(2):97-101
Objective To describe a case of a conjunctivo-corneal mass in a cat associated with acid-fast bacilli.
Methods A 2-year-old female black European Short-Hair cat, living outdoors in a suburban environment in Italy, was referred for evaluation of a nodular, vascularized mass of 2 weeks duration. The mass involved the dorsal bulbar conjunctiva at the temporal canthus of OS and invaded the sclera and cornea. Routine ophthalmic and systemic examination, serologic testing, cytology and histology of the mass were performed. Mycobacterium specific polymerase chain reaction (PCR) of variable regions 1, 2 and 3 of the 16S ribosomal RNA (rRNA) gene was also performed.
Results Neutrophils, lymphocytes, macrophages and giant cells with intracytoplasmic acid-fast bacilli were seen on cytological examination. The histological examination confirmed the presence of a granulomatous lesion with acid-fast bacilli within macrophages. Bacteriological culture of the material from the lesion was negative for Mycobacterium spp. Mycobacterium 16S rRNA gene specific PCR was positive.
A diagnosis of feline leprosy was made. The owners refused any treatment, and 1 year later the lesion was still present.
Conclusions Veterinary ophthalmologists should be aware of conjunctivo-corneal leproma as an unusual symptom of leprosy. 相似文献
Methods A 2-year-old female black European Short-Hair cat, living outdoors in a suburban environment in Italy, was referred for evaluation of a nodular, vascularized mass of 2 weeks duration. The mass involved the dorsal bulbar conjunctiva at the temporal canthus of OS and invaded the sclera and cornea. Routine ophthalmic and systemic examination, serologic testing, cytology and histology of the mass were performed. Mycobacterium specific polymerase chain reaction (PCR) of variable regions 1, 2 and 3 of the 16S ribosomal RNA (rRNA) gene was also performed.
Results Neutrophils, lymphocytes, macrophages and giant cells with intracytoplasmic acid-fast bacilli were seen on cytological examination. The histological examination confirmed the presence of a granulomatous lesion with acid-fast bacilli within macrophages. Bacteriological culture of the material from the lesion was negative for Mycobacterium spp. Mycobacterium 16S rRNA gene specific PCR was positive.
A diagnosis of feline leprosy was made. The owners refused any treatment, and 1 year later the lesion was still present.
Conclusions Veterinary ophthalmologists should be aware of conjunctivo-corneal leproma as an unusual symptom of leprosy. 相似文献
664.
The nuclear 28S rRNA and the mitochondrial COII gene were used to establish phylogenetic relationships among species of the family Neanuridae, with special emphasis on species of the subfamily Neanurinae. Phylogenetic analysis was conducted using genetic distances, parsimony and likelihood methods. The D3-D5 fragment of the rRNA gene was very conserved, both in sequence and in secondary structure features. This fragment supplied little information on relationships at this level. The phylogenetic reconstruction based on 1st and 2nd codon positions of the COII gene was partly in accordance with morphological data, but it was discordant for the placement of some species. Relationships among the subfamilies Frieseinae, represented by the Antarctic species Friesea grisea, Pseudachorutinae and Neanurinae were uncertain. The subfamily Neanurinae and its tribes Neanurini and Paleonurini were shown as monophyletic taxa. Relationships between three species of the genus Bilobella were in accordance with morphological and biochemical data. Relationships between genera within the Neanurini were more controversial. In accordance with morphological hypotheses, a basal position of Thaumanura was suggested, but the molecular data placed Neanura muscorum in a derived position, in sharp contrast with morphological evidence. A close relationship was suggested between Deutonura conjuncta, Cansilianura malatestai and Lathriopyga longiseta. The disagreement between molecular and morphological data suggests that one or both data sets might be affected by a certain degree of homoplasy and that these data should be interpreted with caution in phylogenetic reconstructions. 相似文献
665.
666.
667.
Paolo Guidetti Piero Addis Fabrizio Atzori Simona Bussotti Antonio Cal Alessandro Cau Jean‐Michel Culioli Giuseppe De Lucia Antonio Di Franco Manfredi Di Lorenzo Maria Cristina Follesa Vittorio Gazale Giorgio Massaro Francesco Mura Augusto Navone David Pala Pier Augusto Panzalis Antonio Pusceddu Alberto Ruiu Angelo Cau 《水产资源保护:海洋与淡水生态系统》2019,29(4):537-545
668.
Massimo Genovese Concetta Imperatore Marcello Casertano Anna Aiello Francesco Balestri Lucia Piazza Marialuisa Menna Antonella Del Corso Paolo Paoli 《Marine drugs》2021,19(10)
An in-depth study on the inhibitory mechanism on protein tyrosine phosphatase 1B (PTP1B) and aldose reductase (AR) enzymes, including analysis of the insulin signalling pathway, of phosphoeleganin, a marine-derived phosphorylated polyketide, was achieved. Phosphoeleganin was demonstrated to inhibit both enzymes, acting respectively as a pure non-competitive inhibitor of PTP1B and a mixed-type inhibitor of AR. In addition, in silico docking analyses to evaluate the interaction mode of phosphoeleganin with both enzymes were performed. Interestingly, this study showed that phosphoeleganin is the first example of a dual inhibitor polyketide extracted from a marine invertebrate, and it could be used as a versatile scaffold structure for the synthesis of new designed multiple ligands. 相似文献
669.
Modelling giant red shrimp larval dispersal in the Sardinian seas: density and connectivity scenarios 下载免费PDF全文
Francesco Palmas Antonio Olita Piero Addis Roberto Sorgente Andrea Sabatini 《Fisheries Oceanography》2017,26(3):364-378
The connectivity and three‐dimensional (3D) dispersion of the larvae of giant red shrimp, potentially released from known spawning areas along the Sardinia slope in the western Mediterranean Sea, were assessed using Lagrangian simulations forced by a 3D submesoscale permitting a regional ocean model. Biophysical simulations using the hydrodynamic conditions of the year 2012 were run to track propagules released from known spawning areas during the spawning period (May to September). Passive transport (PT) and vertical migration (VM) scenarios were tested, each with two possible pelagic larval durations (PLDs) of 21 or 42 days. Dispersion of propagules in the PT and VM scenarios differed in terms of travelled distance, export out of the domain (larger for VM), and depth distribution (shallower and bimodal for VM due to the larger variability of encountered currents). Connectivity patterns were investigated among eight release areas, and four predetermined Eco‐Regions, and results showed strong connectivity among the North‐Western (NW), Western (W), and Southern (S) regions of Sardinia, whereas the Eastern region was more segregated. Differences in connectivity patterns among scenarios were related mainly to the tendency of greater retention of propagules in the release area for the PT scenarios. This finding, together with existing hypotheses of vertical migration likely occurring during first egg‐larval phases, suggest that the VM scenarios are the most probable of the two hypotheses tested. Strong connectivity between the W and S sides of Sardinia and the relative isolation of the E side could have significant implications for the protection of this important resource. 相似文献
670.
Valerio Bartolino Francesco Colloca Lorna Taylor Gunnar Stefansson 《Fisheries Research》2011,107(1-3):75-83
An age-length structured model was built for European hake Merluccius merluccius in the central Mediterranean Sea using Gadget. This analytical framework allowed to integrate multiple sources of information, including fisheries-dependent and fisheries-independent data, collected at different scales and aggregation levels. The model includes the two main fisheries targeting hake in the area, the trawl and the gillnet fisheries, and account for differences in their selectivity and effort. Alternative models were used for testing different assumptions on recruitment and growth. The model is then used to predict the main trajectory of the stock during the next years and to evaluate the potential effects of implementing closed areas management scenarios in the hake nurseries as a specific tool to reduce fishing mortality on recruits. The modelling framework presented performed successfully also in a commercial landing data limited context, common for the Mediterranean. Our results provide statistical support for fast growth and multiple recruitment events assumptions. Including both these key features represent an unprecedented improvement of modelling hake population dynamics in the Mediterranean. We found that the reduction in the fishing effort that characterized the fisheries in the study area during the last few years, coupled with fast recovery abilities of the hake stock, has the potentiality to allow a moderate increase of the stock during the next years. Interestingly, our simulations show that the positive effects which might be expected from protecting hake nursery grounds are only marginally related to a reduction in hake recruits fishing mortality. Although our model relies on assumptions and surely represents an over-simplification of the real world, it still contributed to improve our understanding of the temporal dynamics of one of the most valuable fish stocks in the central Mediterranean. 相似文献