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Classification of Dutch and German avian reoviruses by sequencing the sigma C protein 总被引:1,自引:0,他引:1
Kant A Balk F Born L van Roozelaar D Heijmans J Gielkens A ter Huurne A 《Veterinary research》2003,34(2):203-212
We have amplified, cloned and sequenced (part of) the open reading frame of the S1 segment encoding the sigma C protein of avian reoviruses isolated from chickens with different disease conditions in Germany and The Netherlands during 1980 up to 2000. These avian reoviruses were analysed phylogenetically and compared with sequences of avian reoviruses in the Genbank database. The avian reoviruses could be grouped in 5 different genotyping clusters and this classification was identical when the sequences were compared of the 5' end, the 3' end or the whole open reading frame of the sigma C protein. Therefore sequencing of either part of the gene encoding the sigma C protein seems to be reliable for classification. We were unable to identify a correlation between sigma C sequences of the avian reoviruses and the disease condition they were isolated from. The sequences found in The Netherlands and in Germany are, like those in Taiwan, more dispersed than the known avian reovirus sigma C sequences in the USA and Australia. We did not establish temporal or geographic differences in the avian reoviruses studied. 相似文献
13.
Perineal hernias almost exclusively affect male dogs (sexually intact or castrated). Factors that may contribute or predispose a dog to perineal hernias include tenesmus, pelvic musculature variations (male vs female), and gonadal hormone influence. In dogs with perineal hernias and tenesmus it is important to include rectal and prostatic diseases, including paraprostatic cysts, in the differential list of potential underlying causes. Surgical correction of the perineal hernia with a perineal herniorrhaphy is indicated. Successful treatment depends on the degree of preexisting neurologic alterations, meticulous surgical technique, and identification and correction of underlying contributing factors. Paraprostatic cysts develop predominantly in sexually intact medium to large breed dogs. These cysts are thin-walled structures often attached to the prostatic dorsal midline. Osseous metaplasia of paraprostatic cysts may occur. Clinical signs of tenesmus often result from compression on adjacent structures (urinary bladder and colon), and tenesmus may contribute to the development of perineal hernias. Preferred treatment of a paraprostatic cyst is surgical removal and castration. This report describes a dog with bilateral perineal hernias and a large mineralized paraprostatic cyst that was identified as a possible contributing factor to the hernias. 相似文献
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Early dexamethasone treatment after implantation of a sciatic-nerve cuff decreases the concentration of substance P in the lumbar spinal cord of rats with neuropathic pain 下载免费PDF全文
Francis Beaudry Christiane Girard Pascal Vachon 《Canadian journal of veterinary research》2007,71(2):90-97
The main purpose of this study was to evaluate the effects of early dexamethasone treatment on pain-related peptides at an early stage in the development of neuropathic pain induced by implantation of a sciatic nerve cuff in Sprague Dawley rats (body weight 250 to 350 g). The rats were tested for touch sensitivity with the use of von Frey filaments before and 3 d after cuff implantation (n = 12) or sham surgery (n = 6). Half of the cuff-implanted rats received dexamethasone, 1 mg/kg intraperitoneally, 1 h after surgery. Spinal cords were collected on the 3rd day after surgery, and the lumbar enlargement was processed for the detection of selected peptides (neurotensin, substance P, cholecystokinin [CCK], vasoactive intestinal peptide, and calcitonin gene-related peptide) by means of liquid chromatography and tandem mass spectrometry. The right sciatic nerve of each rat was collected, fixed, and stained for histopathological evaluation. Except for neurotensin, all the peptides showed an increased concentration with neuropathic pain; however, the differences were significant (P < 0.05) only for substance P and CCK. In the animals treated with dexamethasone, mechanical allodynia was less pronounced (P < 0.01), and only the concentration of substance P was decreased significantly (P < 0.05). Sciatic nerve sections showed a decrease in C (P < 0.01) and Adelta (P < 0.03) fibres with neuropathic pain and a nearly normal percentage of C fibres after dexamethasone treatment. The dexamethasone-treated animals also had less inflammation detectable microscopically at the nerve constriction site compared with cuff-implanted animals that were not treated with dexamethasone. Our results suggest that in the early stages of neuropathic pain induced by an inflammatory process, dexamethasone may be a useful treatment and that substance P plays an important role in pain perception. 相似文献
16.
Susan J. Sprague Marie-Hélène Balesdent Hortense Brun Helen L. Hayden Stephen J. Marcroft Xavier Pinochet Thierry Rouxel Barbara J. Howlett 《European journal of plant pathology / European Foundation for Plant Pathology》2006,114(1):33-40
Resistance of Brassica napus (oilseed rape, canola) conferred by three different major resistance genes has been overcome by changes in virulence of Leptosphaeria maculans populations in France and Australia. In South Australia where B. napus cultivars with major gene resistance derived from Brassica rapa ssp. sylvestris were grown extensively, resistance was rendered ineffective within 3 years of commercial release of the cultivar. Disease
severity was higher on cultivars with sylvestris-derived resistance than cultivars with polygenic resistance. This Australian situation is compared to that in France, where
resistance conferred by the Rlm1 gene was overcome nation-wide in 5 years under commercial cropping practices, and also where a source of resistance introgressed
into B. napus from B. juncea was rendered inefficient in 3 years in experimental field plots near Rennes. 相似文献
17.
R. Delourme A. M. Chèvre H. Brun T. Rouxel M. H. Balesdent J. S. Dias P. Salisbury M. Renard S. R. Rimmer 《European journal of plant pathology / European Foundation for Plant Pathology》2006,114(1):41-52
The most common and effective way to control phoma stem canker (blackleg) caused by Leptosphaeria maculans in oilseed rape (Brassica napus) is through the breeding of resistant cultivars. Race specific major genes that mediate resistance from the seedling stage
have been identified in B. napus or have been introgressed from related species. Many race specific major genes have been described and some of them are probably
identical in B. napus (allotetraploid AACC) and the parental species B. rapa (diploid AA). More work is needed using a set of well-characterised isolates to determine the number of different major resistance genes
available. In some B. napus cultivars, there is resistance which is polygenic (mediated by Quantitative Trait Loci) and postulated to be race non-specific.
Many of these major genes and Quantitative Trait Loci for resistance to L. maculans have been located on B. napus genetic maps. Genes involved in race specific and polygenic resistance are generally distinct. 相似文献
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19.
A simple trapping procedure for concentration of volatile organic compounds present in a head space and the subsequent analysis of the retained compounds by combined gas chromatographymass spectrometry is described. The procedure was used to study the organic metabolites evolved from various soils when they were amended with glucose and incubated under argon. A number of organic acids, alcohols, aldehydes and esters were evolved during a 4 week period. Less than 10 per cent of the added carbon was recovered as organic compounds, which was largely due to poor recovery of volatile organic acids from soil. Some implications of the observations to microbial ecology are discussed. 相似文献
20.
Confronting collinearity: comparing methods for disentangling the effects of habitat loss and fragmentation 总被引:1,自引:1,他引:0
Adam C. Smith Nicola Koper Charles M. Francis Lenore Fahrig 《Landscape Ecology》2009,24(10):1271-1285
Estimating the relative importance of habitat loss and fragmentation is necessary to estimate the potential benefits of specific
management actions and to ensure that limited conservation resources are used efficiently. However, estimating relative effects
is complicated because the two processes are highly correlated. Previous studies have used a wide variety of statistical methods
to separate their effects and we speculated that the published results may have been influenced by the methods used. We used
simulations to determine whether, under identical conditions, the following 7 methods generate different estimates of relative
importance for realistically correlated landscape predictors: residual regression, model or variable selection, averaged coefficients
from all supported models, summed Akaike weights, classical variance partitioning, hierarchical variance partitioning, and
a multiple regression model with no adjustments for collinearity. We found that different methods generated different rankings
of the predictors and that some metrics were strongly biased. Residual regression and variance partitioning were highly biased
by correlations among predictors and the bias depended on the direction of a predictor’s effect (positive vs. negative). Our
results suggest that many efforts to deal with the correlation between amount and fragmentation may have done more harm than
good. If confounding effects are controlled and adequate thought is given to the ecological mechanisms behind modeled predictors,
then standardized partial regression coefficients are unbiased estimates of the relative importance of amount and fragmentation,
even when predictors are highly correlated. 相似文献