Oligoarray analysis of gene expression in ripening Japanese pear fruit     

Chikako Nishitani  Tokurou Shimizu  Hiroshi Fujii  Fumiko Hosaka  Shingo Terakami  Yuri Nakamura  Akihiro Itai  Ayako Yamaguchi-Nakamura  Toshiya Yamamoto 《Scientia Horticulturae》2010

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Detection of Pythium aphanidermatum in tomato using loop-mediated isothermal amplification (LAMP) with species-specific primers     

Shiro Fukuta  Reiko Takahashi  Satoru Kuroyanagi  Noriyuki Miyake  Hirofumi Nagai  Hirofumi Suzuki  Fujio Hashizume  Tomoko Tsuji  Hiromi Taguchi  Hideki Watanabe  Koji Kageyama 《European journal of plant pathology / European Foundation for Plant Pathology》2013,136(4):689-701

A loop-mediated isothermal amplification (LAMP) reaction with a primer set designed from the rDNA ITS sequence of P. aphanidermatum was developed. Results of a specificity test using 57 strains of Pythium spp. indicated that the LAMP assay gave no cross reactions in other 39 Pythium species, 11 strains of Phytophthora spp. and eight other soil borne pathogens. The detection limit was 10 fg of genomic DNA, which was ten times the sensitivity of the polymerase chain reaction. The LAMP assay was applied to hydroponic solution samples from tomato fields, and the results were compared to those of the conventional plating method. LAMP was observed to be effective for the specific detection of P. aphanidermatum. Furthermore, P. aphanidermatum was detected directly in tomato roots infected with P. aphanidermatum without DNA extraction. The LAMP method established in this study is a simple, sensitive and rapid tool for the detection of P. aphanidermatum.  相似文献   

A putative TetR-type transcription factor AZC_3265 from the legume symbiont Azorhizobium caulinodans represses the production of R-bodies that are toxic to eukaryotic cells     

Jun-ichi Matsuoka  Fumiko Ishizuna  Keigo Kurumisawa  Kengo Morohashi  Tetsuhiro Ogawa  Makoto Hidaka 《Soil Science and Plant Nutrition》2013,59(5):452-459

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Stimulatory effect of aluminum on the growth of tea pollen tubes     

Hiromi Yokota  Ikuyo Takamura  Fumiko Ishikawa  Miwako Ohta  Shigeki Konishi 《Soil Science and Plant Nutrition》2013,59(2):457-461

It is well known that when inorganic elements such as boron (B), calcium, cobalt, and manganese are added to media in combination or alone, they stimulate the germination and/or tube growth of various kinds of pollen (Schmücker 1933; Loo and Hwang 1944; Yamada 1958; Brewbaker and Kwack 1963). Among these elements, the stimulatory effect ofB is the most effective and generally recognized. Aluminum (Al) belongs along with B to Group IIIa in the periodic table, and Fähnrich and Ultlich (1964) reported that Al inhibited the germination and growth of the pollen tube of Petunia hybrida and Antirrhinum majus. However, stimulatory effects of Al on pollen germination or pollen tube growth had not been previously reported.  相似文献   

Genome editing of Nf1, Pten,and Trp53 in neonatal mice induces glioblastomas positive for oligodendrocyte lineage transcription factor 2     

Hiromi Yamamoto  Keisuke Yamamura  Haruka Nagasaki  Takamasa Suzuki  Fumiko Ninomiya  Kenji Matsubara  Naomoto Harada  Shuichi Ohkubo 《Journal of toxicologic pathology》2021,34(4):359

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Effects of flooding depth on growth,morphology and photosynthesis in <Emphasis Type="Italic">Alnus japonica</Emphasis> species     

Fumiko Iwanaga  Fukuju Yamamoto 《New Forests》2008,35(1):1-14

The present study deals with effects of flooding depth on growth, morphology and photosynthesis in Alnus japonica species thorough one field study and two controlled experiments. In the field study performed in Kushiro Mire, Hokkaido Island, Japan, tree heights and stem diameters decreased with an increase in water depth accompanied with the reduction of soil redox potential. In contrast, the rate of multiple stems per individual tree increased. In the controlled experiments for seedlings flooding suppressed the shoot elongation and biomass increment in roots. However, diameter increment around water levels, epicormic shoot development and adventitious root formation were enhanced in flooded seedlings. The photosynthetic rate and stomatal conductance of flooded seedlings also were lowered with an increase in flooding depth. The recovery of the reduced photosynthetic rate and stomatal conductance occurred simultaneously with the advancement of adventitious root formation in the flooded seedlings. These results indicate the importance of a series of morphological changes occurring on stems around water levels in flood tolerance in A. japonica species.  相似文献   

Ammonia volatilization from Vietnamese acid sulfate paddy soil following application of digested slurry from biogas digester     

Nakamura  Masato  Oritate  Fumiko  Yuyama  Yoshito  Yamaoka  Masaru  Dan  Nguyen Phuoc  Hanh  Dang Vu Bich 《Paddy and Water Environment》2018,16(1):193-198

Paddy and Water Environment - In some areas of Vietnam, digested slurry from biogas digesters is discharged into canals without wastewater treatment. In order to reduce environmental pollution,...  相似文献   

Use of a beta 2-adrenergic stimulant (clenbuterol) for eliminating night-time calving.     

T Nakao  K Sato  T Nakamura  K Taguchi  M Moriyoshi  K Kawata 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(1):19-22

To determine whether beta 2-adrenergic stimulant (clenbuterol) would exhibit beneficial effects upon the elimination of night-time calving, 42 Holstein Friesian cows and heifers at the first stage of labour were injected intramuscularly twice with clenbuterol; 300 micrograms at 18:00 and 210 micrograms at 22:00. Other 26 cows and heifers of the same breed at the first stage of labour at 18:00 were not treated and served as controls. All of the treated animals, but one, calved after 5:00 in the next morning, showing a peak of parturition between 5:00 and 10:00. In contrast to this, 42% of the control animals calved at night between 22:00 and 5:00. Thus, the double administrations of clenbuterol were shown to be effective for eliminating night-time calving. The cows treated with clenbuterol tended to show lower incidences of dystocia and retained placenta and a higher first insemination conception rate than the control animals. No adverse effects of the treatment on the viability of newborn calves and milk yields were observed.  相似文献   

Harpinpsta from Pseudomonas syringae pv. tabaci Is Defective and Deficient in Its Expression and HR-inducing Activity     

Fumiko TAGUCHI  Rui TANAKA  Sayuri KINOSHITA  Yuki ICHINOSE  Yoshiyuki IMURA  Salamah ANDI  Kazuhiro TOYODA  Tomonori SHIRAISHI  Tetsuji YAMADA 《Journal of General Plant Pathology》2001,67(2):116-123

To elucidate the role of harpins produced by Pseudomonas syringae, the corresponding hrpZ gene was isolated from P. s. pv. tabaci. The sequence information revealed that this gene carries a serious mutation with 326 bp lacking in the central region and potentially encodes only 140 N-terminal amino acids because of a frame shift. The investigation of biological properties using recombinant harpin indicated harpin_psta was incapable of inducing HR in both host and nonhost plants. Based on an immunoblot analysis to detect harpin from P. s. pathovars in hrp-inducing medium, the truncated harpin_psta was neither expressed nor secreted into the culture medium. These results suggest that harpin is not the sole determinant of the host-parasite specificity in P. s. pv. tabaci. Received 10 August 2000/ Accepted in revised form 21 December 2000  相似文献   

Transition state leading to β-O′ quinonemethide intermediate of p-coumaryl alcohol analyzed by semi-empirical molecular orbital calculation     

Mikiji Shigematsu  Takayuki Kobayashi  Hiroyasu Taguchi  Mitsuhiko Tanahashi 《Journal of Wood Science》2006,52(2):128-133

The radical coupling reaction leading to the β-0′ quinonemethide intermediate of p-coumaryl alcohol was analyzed by semi-empirical molecular orbital calculation with MOPAC2002. By analyzing the radical monomer in a one-electron oxidation, the spin density of the unpaired electron at the 4-oxygen was less than half of the values at the C₁, C₃, C₅, and C_β positions. By analyzing the transition state during the radical coupling reaction, the activation enthalpy was evaluated as 9.76 kcal/mol, which corresponds to the activation energies for the propagation of common vinyl polymers. From the analysis of atomic interactions in the transition state, it was found that the activation enthalpy was largely composed of a high coulombic repulsion between C_β of the first monomer and the phenolic oxygen of the second monomer. After passing the transition state, the two radical monomers formed a metastable quinone-methide intermediate. The optimum conformation of the quinonemethide intermediate was formed from the meta-stable conformation through a second transition state with a small energy barrier. Part of this article was presented at the 46th Lignin Symposium, held in Fukuoka, October 31-November 1, 2002  相似文献