Ultrasonographic findings in two calves with bone sequestration of an extremity     

Taguchi K  Hyakutake K 《The Veterinary record》2012,170(25):649

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Salivary secretory IgA concentrations in beagle dogs     

Kikkawa A  Uchida Y  Nakade T  Taguchi K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(6):689-693

The normal concentrations of salivary secretory IgA (sIgA) were examined, and the response of sIgA to acute stress was evaluated in dogs. Ten clinically healthy beagle dogs familiarized with the method of saliva sampling were used. During the non-stress period, saliva samples were collected between 0800 hr and 1700 hr at 1-hr intervals for 7 consecutive days and analyzed for sIgA concentration. After a 1-day control period, a noise stressor was presented for 15 min between 0845 hr and 0900 hr on 2 consecutive days. Saliva was collected at pre-stress, immediately after, 30 min after and 60 min after the stress. The average sIgA concentration over the 2-day period was compared with the control value. Environmental stimuli were restricted. During the non-stress period, significant variations were observed during the diurnal pattern, in which sIgA increased in the morning and then decreased; and the day-to-day variations were significant except at 0800 hr and 0900 hr. During the stress experiments, the sIgA concentration decreased significantly, immediately after and 30 min after the noise stress, and then increased to the same level as the control value by 60 min after the stress. When estimating the effectiveness of salivary sIgA as a marker of stress in dogs, the appropriate time for saliva sampling appears to be in the morning. Salivary sIgA was deemed potentially useful as a marker of stress in dogs.  相似文献   

Studies of certain factors affecting the microenvironment and microflora of the external ear of the dog in health and disease     

Yoshida N  Naito F  Fukata T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(12):1145-1147

A total of 187 dogs, 110 with clinical signs of otitis externa (OE), and 77 without history or clinical signs of OE, were examined microenvironment and microbiological analysis of their ear exudates made. The aural temperature and humidity of 160 dogs were measured. There were no significant difference between healthy dogs and OE dogs. German shepherd showed relatively lower temperature (p<0.01) and higher humidity (p<0.01). The mean log(10) number of microbial organisms of ears of OE dogs (4.16 +/- 0.31 cfu/g) was significantly increased, compared to that from the ears of non-OE group (2.55 +/- 0.24 cfu/g). Pseudomonas spp. and Proteus spp. were detected only from OE dogs. In addition, three enterotoxigenic Staphylococcus aureus were isolated from ear specimens.  相似文献   

Immunohistochemical localization of transforming growth factor alpha in regenerating rat liver   总被引：1，自引：0，他引：1  

Okada M  Sakairi T  Kusakabe M  Goto K  Tsuchiya T  Sugimoto J  Sano F  Mutai M  Morohashi T  Kobayashi K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(11):1045-1048

Immunohistochemical localization of TGF-alpha and cell proliferation kinetics during liver regeneration after two-thirds partial hepatectomy (PH) were investigated. Twenty-four to 72 hr after PH, appreciable increase in the number of TGF-alpha-positive hepatocytes was observed in zones 1 and 2. At the peak at 36 hr, almost all positive cells were stained in their nuclei. Considerable increase in the BrdU labeling index was observed 24-36 hr after PH with a peak at 24 hr in zones 1 and 2. These results indicated an association between TGF-alpha expression and hepatocyte regeneration. It is suggested that immunohistochemical localization of TGF-alpha may be a useful marker of cell proliferation activity in rat liver.  相似文献   

Flagellin from Pseudomonas syringae pv. tabaci induced hrp-independent HR in tomato     

Mizuri Marutani  Fumiko Taguchi  Rena Shimizu  Yoshishige Inagaki  Kazuhiro Toyoda  Tomonori Shiraishi  Yuki Ichinose 《Journal of General Plant Pathology》2005,71(4):289-295

We have previously shown that flagellin of Pseudomonas syringae pv. tabaci is an elicitor that induces a hypersensitive reaction (HR) in nonhost tomato cells. Flagellin is the major HR elicitor produced by this pathogen, as shown by the inability of a flagellin-defective mutant, ΔfliC, to induce HR. Also, a ΔfliD mutant that secretes large amounts of monomer flagellins induces a strong HR in tomato. In this study, the possible involvement of an Hrp type III secretion system (TTSS) in flagellin-induced HR was investigated using flagella-defective mutants or Hrp TTSS-defective mutants. The hrcC gene encodes HrcC protein, which is required for Hrp pilus formation in the outer membrane. An hrcC mutation, introduced into the wild-type, ΔfliC, and ΔfliD mutants of P. syringae pv. tabaci did not affect swimming motility or flagellin secretion, whereas all ΔhrcC, ΔfliC, and ΔfliD mutants lost the ability to cause disease on host tobacco leaves. However, the ΔhrcC mutant and the ΔfliD/ΔhrcC double mutant were still able to induce HR cell death, expression of one of the defense-related genes hsr203J, and the generation of hydrogen peroxide in nonhost tomato cells. Thus, flagellin is required for both pathogenicity in host tobacco and HR in nonhost tomato. On the other hand, hrp TTSS is necessary for pathogenicity on host tobacco but is not indispensable to induce HR in nonhost tomato. These results clearly show that flagellin-induced HR is hrp-independent in tomato.The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under the accession number AB049570  相似文献   

Defense responses of Arabidopsis thaliana inoculated with Pseudomonas syringae pv. tabaci wild type and defective mutants for flagellin (ΔfliC) and flagellin-glycosylation (Δorf1)     

Yasuhiro Ishiga  Kasumi Takeuchi  Fumiko Taguchi  Yoshishige Inagaki  Kazuhiro Toyoda  Tomonori Shiraishi  Yuki Ichinose 《Journal of General Plant Pathology》2005,71(4):302-307

Flagellin, an essential component of the bacterial flagellar filament, is capable of inducing a hypersensitive response (HR), including cell death, in a nonhost plant. A flagellin-defective mutant (ΔfliC) of Pseudomonas syringae pv. tabaci lacks both the flagellar filament and motility, whereas a flagellin-glycosylation-defective mutant (Δorf1) retains the flagellar filament but lacks the glycosyl modification of flagellin protein. To investigate the role of flagellin protein and its glycosylation in the interaction with its nonhost Arabidopsis thaliana, we analyzed plant responses after inoculation with these bacteria. Inoculation with wild-type P. syringae pv. tabaci induced HR, with the generation of reactive oxygen species and cell death. In contrast, inoculation with either ΔfliC or Δorf1 mutant induced a low level of HR, and inoculated leaves developed a disease-like yellowing. These mutant bacteria multiplied better than the wild-type bacteria in A. thaliana. These results indicate that A. thaliana expresses a defense reaction in response to the bacterial flagellin with its glycosyl structure.  相似文献   

Quantitative Histochemical Study of Perineural Nets in the Retrosplenial Cortex of Albino Rats     

W. Mubarak    R. Sayed  A. Ohtsuka    T. Taguchi    T. Murakami 《Anatomia, histologia, embryologia》2005,34(S1):35-35

Thyrocalcitonin cells (C cells) were predominantly demonstrated in the ultimobranchial (UB) structures of the thyroid gland of one-humped camels. The UB population was observed as clear cells, either singly distributed or as groups of cells between the component cellular elements lining the UB structures. They displayed various shapes: oval, pear-like, rounded, cubical, columnar and or ellipsoid. Those populating the cell nests or solid cellular masses were almost polygonal or ovoid in shape. The cytoplasm contained well-developed golgi complexes, several mitochondria and characteristically presented rounded or oval membrane-bound electron dense granules of different sizes. The latter granules were irregularly distributed within the cytoplasm especially at the vascular pole. The nucleus of C cells was relatively large, indented, occupying a more or less central position in the cytoplasm. It presented various chromatin contents; the heterochromatin variety was predominantly demonstrated at the marginal zone. However, the euchromatin occupied a more central position within the nucleus.  相似文献   

Evidence for estrogen receptor expression in the bursal epithelial cells of chicks     

Young-Ha SHIN  Fumiko TAKAGI  Sachi SUGITA  Song HAN  Takehito TSUJI  Asaki ABE  Yasuhiro KONDO 《Animal Science Journal》2005,76(3):255-259

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Generation of transgenic hybrid aspen that express a bacterial gene for feruloyl-CoA hydratase/lyase (<Emphasis Type="Italic">FerB</Emphasis>), which is involved in lignin degradation in <Emphasis Type="Italic">Sphingomonas paucimobilis</Emphasis> SYK-6     

Shinya?KajitaEmail author  Fumiko?Honaga  Mikiko?Uesugi  Yosuke?Iimura  Eiji?Masai  Shinya?Kawai  Masao?Fukuda  Noriyuki?Morohoshi  Yoshihiro?Katayama 《Journal of Wood Science》2004,50(3):275-280

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Anesthetic and cardiopulmonary effects of total intravenous anesthesia using a midazolam, ketamine and medetomidine drug combination in horses     

Yamashita K  Wijayathilaka TP  Kushiro T  Umar MA  Taguchi K  Muir WW 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(1):7-13

The anesthetic and cardiopulmonary effects of midazolam, ketamine and medetomidine for total intravenous anesthesia (MKM-TIVA) were evaluated in 14 horses. Horses were administered medetomidine 5 microg/kg intravenously as pre-anesthetic medication and anesthetized with an intravenous injection of ketamine 2.5 mg/kg and midazolam 0.04 mg/kg followed by the infusion of MKM-drug combination (midazolam 0.8 mg/ml-ketamine 40 mg/ml-medetomidine 0.1 mg/ml). Nine stallions (3 thoroughbred and 6 draft horses) were castrated during infusion of MKM-drug combination. The average duration of anesthesia was 38 +/- 8 min and infusion rate of MKM-drug combination was 0.091 +/- 0.021 ml/kg/hr. Time to standing after discontinuing MKM-TIVA was 33 +/- 13 min. The quality of recovery from anesthesia was satisfactory in 3 horses and good in 6 horses. An additional 5 healthy thoroughbred horses were anesthetized with MKM- TIVA in order to assess cardiopulmonary effects. These 5 horses were anesthetized for 60 min and administered MKM-drug combination at 0.1 ml/kg/hr. Cardiac output and cardiac index decreased to 70-80%, stroke volume increased to 110% and systemic vascular resistance increased to 130% of baseline value. The partial pressure of arterial blood carbon dioxide was maintained at approximately 50 mmHg while the arterial partial pressure of oxygen pressure decreased to 50-60 mmHg. MKM-TIVA provides clinically acceptable general anesthesia with mild cardiopulmonary depression in horses. Inspired air should be supplemented with oxygen to prevent hypoxemia during MKM-TIVA.  相似文献