氧化油脂对奥尼罗非鱼生长和抗氧化性能的影响   总被引：4，自引：0，他引：4  

黄凯  阮栋俭  战歌  甘西  罗永巨  龚竹林 《淡水渔业》2006,36(6):21-24

通过饲喂不同过氧化值油脂的饲料,研究奥尼罗非鱼(Oreochromisniloticus×O·aureus)的生长性能、肝胰腺抗氧化能力与氧化油脂的关系。试验结果表明:添加氧化油脂导致罗非鱼的增重率和饲料转化率显著下降(P<0·05),肝体比显著增加,但肝脂率、肌脂率的变化不明显(P>0·05);相对于过氧化值较低的饲料,饲料过氧化值(POV)为73·44mmol/kg时,肝胰腺中还原型谷胱甘肽(GSH)含量、超氧化物歧化酶(SOD)活力明显增高(P<0·05);氧化油脂对肝胰腺中丙二醛(MDA)含量无明显影响(P>0·05)。  相似文献   

食蚊鱼CYP19a基因的克隆与序列分析     

甘为  方展强 《水产学报》2016,40(10):1542-1555

硬骨鱼类CYP19基因与生物的性别分化和激素调节相关,因此可开发用来探究环境激素污染与基因表达的关系。本研究首次克隆和分析了食蚊鱼CYP19a cDNA的全系列,为将CYP19基因作为监测环境激素生物标志物的研究提供了全面的实验数据。根据CYP19a基因c DNA保守区域设计引物,扩增保守区域并测序。采用RACE法扩增食蚊鱼CYP19a基因c DNA序列全长,对其蛋白序列进行同源性分析,并将序列应用于CYP19a mRNA转录水平的RT-PCR法检测中。成功克隆食蚊鱼CYP19a基因全长,获得CYP19a基因总长为2020 bp,ORF为238~1791 bp,共编码518个氨基酸,对其编码的蛋白质进行有关信号肽、跨膜螺旋、亲水性/疏水性、一级结构、二级结构和三级结构分析,与其他硬骨鱼类底鰆、青鰆、平鲷、鲫、鲤和斑马鱼的性腺CYP19a基因作同源性比较,其基因相似度分别为93%、84%、84%、71%、71%和66%。用MEGA6.0软件对19个物种的CYP19a基因进行聚类分析,食蚊鱼CYP19a基因与底鰆、青鰆同源性最高,说明芳香化酶在进化上相对保守。确定从食蚊鱼性腺所克隆的CYP19a基因是芳香化酶基因,证明食蚊鱼的芳香化酶是由CYP19a和CYP19b两种基因编码的。食蚊鱼卵巢芳香化酶具有3个高度保守的片段,并具有催化活性。  相似文献   

棘胸蛙人工饲养技术     

徐鹏飞 《中国水产》2000,292(3)

棘胸蛙俗称石蛙,是一种牺居在深山密林山涧溪流中的珍稀两栖动物,素有“蛙中皇后”之称。石蛙因长期处于野生状态,资源有限,产品供不应求,价格日益昂贵。因此,积极开拓这种名贵的优良品种,变野生为人工驯养,是一项新兴的养殖业,具有十分广阔的前景。石娃是一种大型肉用蛙,体重可达5皿克以上,肉质洁白脆嫩,味极鲜美,营养丰富,含有高蛋白、氨基酸、多种维生素和矿物质;具有清热解毒、滋补强身、防癌抗癌之功效。目前我国人工养殖的姓类品种,主要有古巴牛蛙和美国青蛙;但这两种蛙的出肉率、肉质鲜味和蛙皮深加工等方面都不及…  相似文献   

东海海鳗基于COⅠ基因片段的遗传多样性分析     

李鹏飞  徐开达  朱文斌  张亚洲 《水产养殖》2013,34(4)

以东海海鳗背部肌肉的线粒体DNA为模板,采用PCR技术对14个个体的CO Ⅰ基因片段进行序列分析.通过双向测序,除去两端的部分序列,最终得到了684 bp的基因片段.经分析,14个序列共有2个单倍型(GenBank登录号:HM068279,HM068292).在14个个体中,仅检测到1个变异位点,为C/T转换.MEGA软件统计表明,密码子的第一位4种碱基的分布比较均一,密码子的第二位T的含量比较高(T=42.1％),密码子的第三位出现G的含量较低的偏倚现象(G=11.0％).利用DNAsp软件对东海区海鳗基于COⅠ基因序列进行了遗传变异参数统计,结果显示,单倍型多样性为0.143,核苷酸多样性指数为0.00021,平均核苷酸差异数为0.143.研究结果表明,海鳗CO Ⅰ基因序列的变异很小,并且处于相当低的遗传多样性水平,对研究海鳗的种群结构和遗传多样性具有一定的应用价值.  相似文献   

柘林湾网箱养殖海域溶解氧分布及其影响因素   总被引：5，自引：0，他引：5  

甘居利  林钦  李纯厚  贾晓平  林燕棠  王小平  杨美兰 《海洋水产研究》2001,22(1):69-74

根据１９９８年７月在南海东北部柘林湾的调查数据，探讨网箱养殖海域溶解氧的分布及其与其他环境因子间的关系，结果显示夏季海水溶解氧浓度为３.03-7.86mg/L( 平均５．３０）,退潮时（平均５.71)高于涨潮时（平均４.65)涨潮时网箱区内贫氧（ ＜０．mg/L).多元逐步回归分析表明，对溶解氧浓度有重要影响的水质因子为pH和水温，其次为盐度，ＣＯＤ和营养盐，它们的变化与ＤＯ的变化有较好的相关性，区域比较表明，海水溶解氧浓度与观测僧置到网箱区的距离呈正相关（Ｐ＜０．０２），与底质硫物，ＰＯ４－Ｐ含量呈负相关（Ｐ＜０．０５）,溶解氧浓度偏低与养殖环境的污染有直接关系。  相似文献   

异型雄性罗氏沼虾遗传多样性的微卫星分析     

吕敏  黄光华  李旻  杨琼  卢小花  甘晖  阮志德  黄立斌  杨彦豪  卢天和  马华威 《水产科学》2019,(3):355-360

利用微卫星DNA标记研究3种近亲交配形态型雄性罗氏沼虾遗传多样性,所用虾样均采自国家级(南宁)罗氏沼虾良种场,共150尾成年近亲交配的雄虾,其中蓝色长臂型、橙色长臂型、小个体型各50尾。研究结果表明,3组样品的生长发育差异显著,蓝色长臂型、橙色长臂型、小个体型各组中微卫星位点的总等位基因分别为24、28和22,3组样品所有位点的平均观察杂合度为0.474～0.556。其平均群体内近交系数为-0.0189,说明杂合子过剩,对比分析和群体间分化系数表明,3组雄虾遗传差异明显,最大遗传间距位于蓝色长臂型和小个体型,最小的遗传间距位于蓝色长臂型和橙色长臂型。研究结果将为罗氏沼虾优质父本选育和调控雄性遗传发育提供基础性遗传信息。  相似文献   

Survival and growth of the sea cucumber Holothuria leucospilota Brandt: a comparison between suspended and bottom cultures in a subtropical fish farm during summer     

Zonghe Yu  Chaoqun Hu  Yi Zhou  Haipeng Li  Pengfei Peng 《Aquaculture Research》2012,44(1):114-124

The feasibility of co‐culturing the sea cucumber Holothuria leucospilota Brandt in a subtropical fish farm was investigated in a field study. Sea cucumbers were cultured in the fish farm in cages suspended at 4 m deep (suspended culture) and directly on the seafloor (bottom culture). The survival and growth of the sea cucumbers were monitored twice during the 3‐month, summer experimental period (May 26–August 14, 2010). Results showed that the suspension‐cultured sea cucumbers exhibited excellent survival rate (100%) during the whole study period. There also occurred no mortality in the bottom‐cultured sea cucumbers during the first culture period (May 26–July 13); but all these died from anoxia caused by water column stratification during the second culture period (July 14–August 14). The specific growth rate of the bottom‐cultured sea cucumbers (1.05 ± 0.21 % day^?1) was nearly double that of the suspended culture animals (0.57 ± 0.21 % day^?1) during the first culture period, and the growth rates of the suspended culture sea cucumbers in the second culture periods (0.46 ± 0.24 % day^?1) was only a little lower than that of the first period. The sea cucumbers H. leucospilota could ingest and assimilate sediment with high organic matter content with an average assimilation efficiency of 14.9 ± 3.9%. This study indicated that fish farm detritus can be effectively used as a food source for the sea cucumber and that it can be turned into a valuable secondary crop in the form of the sea cucumber biomass.  相似文献   

Autophagy and myocardial ischemia/reperfusion injury     

LIU You-bin  YANG Shu-sen  FAN Ying  GAN Run-tao 《园艺学报》2009,25(12):2478-2482

Autophagy is a lysosome-dependent degradative pathway which is characterized by cytoplasmic vacuolization. However, it is not just a simple degradative pathway. Research shows that autophagy is related to many diseases, such as neurodegenerative disease, malignant tumor, ageing, pathogenic microorganism infection, myocardial ischemia/reperfusion injury and so on. Autophagy exactly exists in myocardial ischemia/reperfusion injury, and it becomes a new research hotspot. This review will focus on the occurrence and development of autophagy and its role, signal transduction and research status in myocardial ischemia/reperfusion injury.  相似文献   

Effect of simvastatin on expression of integrin-linked kinase and renal tubulointerstitium injury in rats induced by high fat diet     

DU Xiao-gang  GAN Hua  LVZhi-mei 《园艺学报》2009,25(9):1791-1795

AIM: To explore the effect of simvastatin on expression of integrin-linked kinase (ILK) and the injury of renal tubulointerstitium in the rats induced by high fat diet. METHODS: Fifty-four 6-8 week-old female Wistar rats were randomly assigned to the following three groups: high fat diet group, simvastatin group (rats were fed with high fat diet plus 10 mg·kg-1·d-1 simvastatin) and control group. Six rats in each group were sacrificed at 4th week, 10th week, and the others at 20th week. The injury of renal tubulointerstitium was observed under microscope with HE staining and the expression of renal ILK was determined by Western blotting analysis and immunohistochemistry. Levels of total cholesterol (TC) and triglyceride (TG) in serum were measured by enzymatic colormetric methods. RESULTS: The serum TC and TG levels and the expression of renal ILK significantly increased in both high-fat diet group and simvastatin group, compared to control group at 4th week, reaching a maximum at 20th week (P<0.01). Tubulointerstitium injuries including vacuolar degeneration, syncytial change, clody swelling, necrosis and atrophy of renal tubular epithelial cells, thinning of tubal wall, lumens compensational expansion or even abolition, and inflammatory cell infiltration, interstitial fibrosis were found in both high fat diet group and simvastatin group, compared to control group at 4th week, worsened to a maximum at 20th week. However, all of these ameliorated in simvastatin group, compared to high fat diet group at each time point. CONCLUSION: The results indicate that high-fat diet induces significant lesion of renal tubulointerstitium and increased expression of renal ILK. Simvastatin may play an important role in protecting against tubulointerstitium injury induced by hyperlipoidemia by down-regulating the expression of renal ILK.  相似文献   

观赏竹容器青苗基质开发初步研究     

郭璟  王燕  干甜芳  王洁  应叶青 《北方园艺》2009,(12)

国际市场中要求竹苗的出口不能带土,根据基质选择的原则及评价的标准,配制了8种基质(G1、G2、G3、G4、G5、G6、G7、G8)作为鸡毛竹(S.chinensis Nakai)和黄条金刚竹(Pleioblastus kongosanensis f.aureostriaus)的容器育苗基质.结果表明:在G8中,鸡毛竹、黄条金刚竹的生长情况最好,在G3、G7中,鸡毛竹、黄条金刚竹的生长情况较好,但成本相对较高.在泥炭中加入山核桃壳或煤渣等经济基质材料,降低了成本,改善了基质的通气性和保水性.  相似文献