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11.
Two experiments were conducted to investigate effects of processing on apparent digestibility coefficients (ADCs) of legumes and oilseeds for juvenile silver perch, Bidyanus bidyanus (~49 g). The first experiment evaluated interactive effects of ingredients (lupins or field peas), processing (whole seed; hulls on or hulls off) and extrusion cooking (raw or extruded) on ADCs for juvenile silver perch (~4 g fish?1). The second experiment was a three‐fixed‐factor anova designed to evaluate interactive effects of ingredients (soybean meal or canola meal), extrusion cooking (raw or extruded) and inclusion content (30% or 50% of the diet) on ADCs for juvenile silver perch (~4 g fish?1). Lupin protein was more digestible than that of peas (ADC for crude protein 91% vs. 85% for peas) but the organic matter was less digestible (ADC for organic matter 50% vs. 67% for peas). Dehulling lupins significantly improved ADCs for all indices (dry matter, organic matter, energy and crude protein), but extrusion had no effect because lupins do not contain starch or heat‐labile anti‐nutrients. Conversely, for starch‐rich peas that contain heat‐labile trypsin inhibitors, both dehulling and extrusion significantly improved ADCs. Digestibility of soybean meal was much higher than that of canola meal. For soybean meal, neither processing, content nor their interaction affected digestibility but extrusion improved ADCs for dry matter, organic matter and energy but there was an interaction with content. Although higher overall, digestibility for these indices declined with increasing content for extruded product while there were only minor effects of inclusion for raw product. Benefits of extrusion were attributed to reductions in anti‐nutrients, including phytic acid. For canola, there were no interactions between extrusion and content for any ADC. Increasing content reduced ADCs for crude protein, dry matter and organic matter but did not effect energy. Surprisingly, extrusion of canola also reduced digestibility for all ADCs. Dehulling improved both lupins and peas. Crude protein for all ingredients was well digested with ingredients ranked: lupins>soybean meal>peas>canola meal. Energy digestibility was best for soybean meal and worst for lupins. Extrusion greatly improved digestibility of peas and to a lesser extent soybean meal, gave no benefits to lupins and was detrimental for canola.  相似文献   
12.
Backcross breeding programs have been used to transfer disease resistance and other traits from one forest tree species to another in order to meet restoration objectives. Evaluating the field performance of such material is critical for determining the success of breeding programs. In eastern North America, The American Chestnut Foundation has a backcross breeding program that uses Chinese chestnut (Castanea mollissima Blume) to introduce resistance of the fungal pathogen chestnut blight [Cryphonectria parasitica (Murr.) Barr.] to the native American chestnut [Castanea dentata (Marsh.) Borkh.]. We compared physiological and morphological characteristics among seedlings of American chestnut, Chinese chestnut, and BC1F3, BC2F3, and BC3F3 hybrid chestnuts during their fourth growing season after field-planting. American chestnut and the BC3F3 breeding generation displayed photosynthetic light-response curves that were similar to each other but different from Chinese chestnut. Rates of photosynthesis were higher for American chestnut and the BC3F3 breeding generation when compared to Chinese chestnut for light levels ≥800 μmol m?2 s?1 photosynthetic photon flux density and for maximum photosynthetic capacity. Leaf morphology variables were not different between American chestnut and any of the breeding generations, but leaf area (on a per leaf basis) of Chinese chestnut was lower than that of any other chestnut type. Our results suggest that backcross breeding can be used to transfer desirable traits for restoration of native species threatened by non-native pathogens.  相似文献   
13.
Two experiments were performed to investigate the digestibility and utilization of carbohydrate sources by Australian snapper Pagrus auratus. In the first experiment, snapper of two different size classes (110 and 375 g) were fed a reference diet containing no starch (REF) or diets containing 150 (PN15), 250 (PN25), 350 (PN35) or 450 g kg?1 (PN45) of 100% gelatinized wheat starch to investigate the interactive effects of fish size and starch inclusion level on apparent organic matter (OM) or gross energy (GE) digestibility (ADC), post‐prandial plasma glucose concentration, hepatosomatic index (HSI) and liver or tissue glycogen content. A second experiment used a 72 h time course study to investigate the ability of larger snapper (300–481 g) to clear an intra‐peritoneal injection of 1 g d ‐glucose kg?1 body weight (BW). Organic matter and GE ADCs declined significantly in both fish sizes as the level of starch increased (PN45energy small fishenergy large fish). There was no interaction between fish size and inclusion level with respect to GE or OM ADCs. Gross energy ADC for both sized fish was described by the linear function GE ADC=104.97 (±3.39)–0.109 (±0.010) × inclusion level (R2=0.86). Hepatosomatic index, liver and muscle glycogen concentrations were significantly elevated in both small and large snapper‐fed diets containing gelatinized starch compared with snapper fed the REF diet. Three‐hour post‐prandial plasma glucose concentrations were not significantly affected by fish size, inclusion level or the interaction of these factors (REF=PN15=PN25=PN35=PN45), and ranged between 1.60 and 2.5 mM. The mean±SD resting level of plasma glucose (0 h) was 2.4±1.1 mM. Circulating levels of plasma glucose in snapper peaked at 18.9 mM approximately 3 h after intra‐peritoneal injection and fish exhibited hyperglycaemia for at least 12–18 h. There were no significant differences between the plasma glucose concentrations of snapper sampled 0, 18, 24, 48 or 72 h after injection (0=18=24=48=72<12< 1<3=6 h), indicating snapper required almost 18 h to regulate their circulating levels of glucose to near‐basal concentrations. Australian snapper are capable of digesting moderate levels of gelatinized wheat starch; however, increasing the dietary content of starch resulted in a reduction in OM and GE digestibility. Smaller snapper appear to be less capable of digesting gelatinized starch than larger fish, and levels above 250 and 350 g kg?1 of diet are not recommended for small and large fish respectively. Snapper subjected to an intra‐peritoneal injection of d ‐glucose have prolonged hyperglycaemia; however, the post‐prandial response to the uptake of glucose from normally digested gelatinized starch appears to be more regulated.  相似文献   
14.
15.
The objective of this study was to quantify the genetic variability and determine the inheritance of Al tolerance in lucerne (or alfalfa) using a 4-parent diallel mating design. Regrowth root length (RRL), along with root length (RL) and total root length (TRL), was measured to indicate relative Al stress response using a hydroponic system in a 3 μM Al solution at pH 4.5. A diallel analysis indicated the significance of general combining ability (gca) variance for RRL but not the specific combining ability (sca) variance; the same result was obtained for TRL but not for RL. For both RRL and TRL, genetic variance appeared to be more important than the environmental variance. For RRL, a strong but non-significant correlation was indicated between parental performance and their gca effects; while mid-parent heterosis and/or over-dominance were detected, as associated with the expressed sca effects in several combinations. These results suggest the complex genetic nature and expression of Al tolerance in the 4-parent diallel crossing system tested. Al-tolerant parent, GAAT‘S’, was the most promising parent, conferring the highest gca effects for RRL as well as for other two characters. The existence of significant gca variance in RRL may also suggest the feasibility of improving Al tolerance through enhanced root regrowth using phenotypic recurrent mass selection to pyramid desirable Al-tolerant genes, focussing on parental lines and/or elite individual plants expressing long regrowth roots.  相似文献   
16.
Concentrations of benomyl greater than 10 ng ml?1 inhibited germination and hyphal growth of the vesicular-arbuscular mycorrhizal fungus Glomus caledonicum on water agar. This effect was attributable to only the activity of methyl-2-benzimidazole carbamate, one of the two hydrolysis products of benomyl. Hyphal growth but not germination was affected by the pH of water agar buffered with 10 mm 2-(N-morpholino)ethane sulphonic acid.  相似文献   
17.
House mice Mus musculus have successfully colonized many temperate and sub-Antarctic islands that are the location for breeding colonies of millions of seabirds. Unlike other introduced mammals, the impact of house mice on seabirds and endemic birds is believed to have been negligible. The breeding ecology of seabirds breeding on Gough Island, central South Atlantic Ocean, was studied for the first time during September 2000 to September 2001. Breeding success of the endangered Tristan albatross Diomedea (exulans) dabbenena and endangered Atlantic Petrel Pterodroma incerta were 27.3 and 19.9% respectively. Mortality of large Tristan albatross and Atlantic petrel chicks was observed, and the pattern of wounds and observations of feeding indicate that introduced mice were responsible for this predation. Breeding numbers of the endemic Gough bunting Rowettia goughensis are mostly found in upland areas of Gough Island where mice are scarce and are restricted to inaccessible cliffs in the lowlands where mice are abundant. This pattern, together with the high predation rates of artificial-eggs in lowland habitats in comparison to the uplands, strongly suggests that mice constrain the distribution of Gough buntings. The results of this study provide the first evidence for the role of house mice as a significant predator of endangered and endemic birds. Further research is required to determine if the observed levels of mice predation are a regular occurrence.  相似文献   
18.
19.
The release of mercury from volcanic eruptions on the Island of Hawaii has been well documented, but the dispersion characteristics have not previously been studied in detail. Samples of lichens were collected from around the island, and subsequent analysis showed levels ranging from < 8 to 59 µg Hg g-1. The distribution of the accumulation of mercury clearly shows that there is a major input from the active eruption sites, but that this is significantly modified by variations in wind patterns. Superimposed on this regional distribution are two localised sites where the mercury accumulation is exceptionally high; this is attributed to the release of steam from the circulation of shallow ground waters.  相似文献   
20.
The endangered Tristan albatross Diomedea dabbenena is restricted to Gough and Inaccessible Islands. The species is killed as bycatch by longline fisheries in the South Atlantic, but the impact of this mortality is unknown. We satellite tracked 38 breeding Tristan albatrosses and assessed the seasonal and annual at-sea distribution of these birds in relation to reported pelagic longline fishing effort. These birds ranged across the South Atlantic from 50°W to 15°E with most (97%) daytime satellite fixes between latitudes 30°S and 45°S. Considerable fishing effort occurred within the same latitudes. Although there was no correlation between their at-sea distributions, there was a broad overlap between birds and fishing effort. Estimated bycatch rates for Tristan albatross and other Diomedea species in the South Atlantic, and the spatio-temporal overlap between birds and hooks, yield a predicted annual mortality of 471-554 birds, sufficient to cause population decreases of 3.6-4.3% per year. An index of bird × hook interactions (proportional density of birds multiplied by number of hooks by decadal period for each 5° square of longitude and latitude) indicated that 47% of annual interactions occurred in areas around Gough Island, and 11% and 15% of interactions in areas of the west and east Atlantic, respectively. There were also within seasonal differences in the key areas of overlap. The fishing fleets of Taiwan and Japan are likely to be responsible for most interactions based upon the reported magnitude of effort expended in the South Atlantic by these fleets. Ensuring that licensed fishing vessels within the Tristan da Cunha Exclusive Economic Zone (EEZ) operate using best-practise mitigation measures and with fisheries observer programs, could reduce the potential bycatch mortality of breeding Tristan albatrosses in this region by nearly one third. Thorough implementation of international agreements is required in areas of the high seas where most remaining interactions are predicted to occur.  相似文献   
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