Haematological modulation and growth of Labeo rohita fingerlings: effect of dietary mannan oligosaccharide,yeast extract,protein hydrolysate and chlorella     

Simi Rose Andrews  Narottam P Sahu  Asim K Pal  Shivendra Kumar 《Aquaculture Research》2009,41(1):61-69

The present study was conducted for 60 days to delineate the efficacy of various dietary immunomodulators like mannan oligosaccharide (MOS), yeast extract (YE), protein hydrolysate (PH) and chlorella (CL) in Labeo rohita fingerlings. Five hundred and eighty‐five L. rohita fingerlings (average weight: 4.15 ± 0.07 g) were randomly distributed in 13 treatment groups with each of three replicates. Thirteen semi‐purified isonitrogenous (crude protein 324.7–332.5 g kg^?1) and isocaloric (17.66–17.80 MJ kg^?1) diets were prepared with three graded levels (1%, 2% or 4%) of immunostimulants, except the control. At the end of the feeding trial, weight gain%, specific growth rate, feed conversion ratio, leucocyte count, erythrocyte count, haemoglobin content, serum protein, globulin, albumin–globulin ratio, nitroblue tetrazolium (NBT) value and survival percentage were evaluated. Growth was significantly higher in the MOS‐fed group. All the immune parameters studied were also recorded higher in the MOS 1%‐supplemented group. The survival percentage after challenging with Aeromonas hydrophila was higher (P<0.05) in the MOS‐, YE‐ and PH‐fed groups and the lowest in the CL‐treated group. It can be concluded that dietary supplementation of MOS at a 1% dietary level promotes growth and survival in L. rohita fingerlings. In contrast, higher inclusion levels of immunostimulants led to an immunosuppressive effect in L. rohita fingerlings.  相似文献   

Dietary Crude Protein,Citric Acid and Microbial Phytase Interacts to Influence the Hemato‐Immunological Parameters of Rohu,Labeo Rohita,Juveniles     

Kartik Baruah  Asim K. Pal  Narottam P. Sahu  Dipesh Debnath  Sona Yengkokpam  Parisa Norouzitallab  Patrick Sorgeloos 《Journal of the World Aquaculture Society》2009,40(6):824-831

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Microbial Degradation of Organophosphate Pesticides: A Review     

Shardendu KUMAR  Garima KAUSHIK  Mohd Ashraf DAR  Surendra NIMESH  Ulrico Javier L&#;PEZ-CHUKEN  Juan Francisco VILLARREAL-CHIU 《土壤圈》2018,28(2):190-208

Pesticides have become an inevitable part of the modern environment as they are widely used in agriculture,household,and public health sectors and,hence,are extensively distributed throughout most ecosystems.Currently,organophosphate pesticides are the most commercially favored group of pesticides,with large application areas all over the world.Depending on their fate,these organophosphorus compounds may become bioavailable for microbial degradation.Environmental microbes,such as Aspergillus,Pseudomonas,Chlorella,and Arthrobacter,are capable of coupling a variety of physical and biochemical mechanisms for the degradation of organophosphate pesticides,including adsorption,hydrolysis of P–O alkyl and aryl bonds,photodegradation,and enzymatic mineralization.Enzymes,such as esterase,diisopropyl fluorophosphatase,phosphotriesterase,somanase,parathion hydrolase,and paraoxonase,have been isolated from microbes to study and understand the catabolic pathways involved in the biotransformation of these xenobiotic compounds.This review highlights various aspects of biodegradation of organophosphate pesticides along with biological and molecular characterization of some organophosphate pesticide-degrading bacteria.  相似文献   

Response of gastrointestinal melatonin,antioxidants, and digestive enzymes to altered feeding conditions in carp (<Emphasis Type="Italic">Catla catla</Emphasis>)     

Palash Kumar Pal  Saumen Kumar Maitra 《Fish physiology and biochemistry》2018,44(4):1061-1073

The purpose of present study was to ascertain whether the response of gastrointestinal (gut) melatonin to altered feeding conditions was related to the levels of different antioxidants and digestive enzymes in the same gut tissues of a sub-tropical carp (Catla catla). Accordingly, the fish were subjected to food deprivation for 4 or 8 days and separately to re-feeding for 4 or 8 or 12 days after deprivation of food for 8 days, and their gut tissue homogenates were used to measure the levels of melatonin, both enzymatic [superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST)] and non-enzymatic [reduced glutathione (GSH)] antioxidants, as well as different digestive enzymes (α-amylase, cellulase, protease, and lipase). Notably, the gut levels of melatonin, SOD, CAT, GPx, and GST underwent gradual increase with the progress of food deprivation, but a sudden fall after restoration of food supply for 4 days and a rise thereafter. Conversely, the activity of all the digestive enzymes significantly decreased after deprivation of food, but started increasing when food supply was reinforced. Gut melatonin concentrations by showing a positive correlation with the titers of different antioxidants (in both food-deprived and re-fed fish groups) and a negative (in food-deprived fish) or a positive (in re-fed fish) correlation with the activity of each digestive enzyme underlined possible physiological interplay between them. Collectively, our findings lend support to the hypothesis that gut melatonin response to altered feeding conditions in carp might be associated with the oxidative status as well as the digestive functions of the gastrointestinal tissues itself.  相似文献   

Water use in rice crop through different methods of irrigation in a sodic soil     

Atul Kumar Singh  Sanjay Arora  Yash Pal Singh  Chhedi Lal Verma  Ajay Kumar Bhardwaj  Navneet Sharma 《Paddy and Water Environment》2018,16(3):587-593

Sodic soils are characterized by high exchangeable sodium on exchange sites, soil pH greater than 8.5, relatively low electrical conductivity, low infiltration rate and dispersed clay. These characteristics restrict the capacity of soil to absorb water, resulting in poor infiltration. Evidently, these soils require application of irrigation water at shorter intervals for crop production. Thus, irrigation strategy for sodic soils differs from that of normal soils. An experiment to determine the suitable irrigation strategy along with methods of application namely: surface (farmer’s practice), sprinkler (double nozzle impact sprinkler), and low-energy water application device (LEWA) were initiated in the year 2012 for rice crop. Irrigation depths of 6 cm in case of surface method and 4 cm in case of sprinkler and LEWA methods were applied at each irrigation event. The irrigation events for rice were scheduled at 2-DAD (days after the disappearance of the ponded water), 3-DAD, and 4-DAD through surface method, and at daily, 1- and 2-day intervals (after initial ponding disappeared) by sprinkler and LEWA methods. Sprinkler and LEWA methods resulted in highest rice yield of 4.4 t ha^?1 in irrigated plots at the 2-day interval which was at par with the highest yielding surface-irrigated plot scheduled at 2-DAD. At the same time, irrigation strategy of 2-day interval through sprinkler and LEWA methods registered water saving to the extent of 30–40% over 2-DAD under surface irrigation method. Results revealed that there could be substantial saving of water and energy (electricity and diesel) through the use of sprinkling devices for irrigating rice under sodic soil environments.  相似文献   

Exploring genetic diversity for heat tolerance among lentil (Lens culinaris Medik.) genotypes of variant habitats by simple sequence repeat markers          下载免费PDF全文

Dharmendra Singh  Chandan K. Singh  Ram Sevak S. Tomar  Ashish K. Chaturvedi  Divya Shah  Arun Kumar  Madan Pal 《Plant Breeding》2016,135(2):215-223

The study was carried out to assess genetic diversity among 119 lentil genotypes grown in different habitats for heat tolerance using morpho‐physiological and reproductive traits and SSR markers. High‐temperature stress was applied at seedling (35/33°C) and anthesis stages (35/20°C) to study the effects on morpho‐physiological and reproductive traits under hydroponic condition, which was compared with non‐stressed and stressed field conditions. A set of 209 alleles were identified by 35 SSR markers among the genotypes. Genetic diversity and polymorphism information content values varied between 0.0494–0.859 and 0.0488–0.844, with mean values of 0.606 and 0.563, respectively. Genotypes were clustered into nine groups based on SSR markers. Morpho‐physiological and reproductive traits under heat stress were found to be significantly different among SSR clusters. These findings suggest that heat adaptation is variable among the genotypes and the tolerant materials can be evolved through hybridization using parents from different clusters with diverse mechanisms of heat tolerance.  相似文献   

Effect of Silicon Fertilization on Growth,Yield, and Nutrient Uptake of Rice     

Sajal Pati  Biplab Pal  Shrikant Badole  Biswapati Mandal 《Communications in Soil Science and Plant Analysis》2016,47(3):284-290

A field experiment was conducted to study the effect of silicon (diatomaceous earth, DE) fertilization on growth, yield, and nutrient uptake of rice during the kharif season of 2012 and 2013 in the new alluvial zone of West Bengal, India. Results showed that application of silicon significantly increased grain and straw yield as well as yield-attributing parameters such as plant height (cm), number of tillers m^?2, number of panicle m^?2, and 1000-grain weight (g) of rice. The greatest grain and straw yields were observed in the treatment T₆ (DE at 600 kg ha^?1 in combination with standard fertilizer practice (SFP). The concentration and uptake of silicon, nitrogen (N), phosphorus (P), and potassium (K) in grain and straw were also greater under this treatment compared to others. It was concluded that application of DE at 600 kg ha^?1 along with SFP resulted increased grain, straw, and uptake of NPK.  相似文献   

Assessment of Anthropogenic Impacts on the Water Quality of Marreco River,Brazil, Based on Principal Component Analysis and Toxicological Assays     

Soraya Moreno Palácio  Fernando Rodolfo Espinoza-Quiñones  Aline Roberta de Pauli  Pitágoras Augusto Piana  Caroline Bressan Queiroz  Simony Camila Fabris  Márcia Regina Fagundes-Klen  Márcia Teresinha Veit 《Water, air, and soil pollution》2016,227(9):307

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How methane feedback response influence redox processes in a tropical vertisol     

Santosh Ranjan Mohanty  Sneh Tiwari  Garima Dubey  Usha Ahirwar  Bharati Kollah 《Biology and Fertility of Soils》2016,52(4):479-490

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Thermal tolerance, oxygen consumption and haemato-biochemical variables of Tor putitora juveniles acclimated to five temperatures     

M. S. Akhtar  A. K. Pal  N. P. Sahu  A. Ciji  P. C. Mahanta 《Fish physiology and biochemistry》2013,39(6):1387-1398

A 30-day acclimation trial was conducted using Tor putitora to elucidate its thermal tolerance, oxygen consumption, haemato-biochemical variables and selected enzymatic activities at five acclimation temperatures (AT). Juveniles of T. putitora were randomly distributed among five treatment groups (20, 23, 26, 29 and 32 ± 0.5 °C). There was a significant curvilinear increase in critical thermal maxima (CT_max) (y = ?0.0693x ² + 1.7927x + 34.628, R ² = 0.996) and lethal thermal maxima (LT_max) (y = ?0.1493x ² + 2.3407x + 35.092, R ² = 0.991) with increasing AT. The oxygen consumption rate increased significantly with increasing AT. The Q ₁₀ values were 1.16 between 20 and 23 °C, 3.09 between 23 and 26 °C, 1.31 between 26 and 29 °^C and 1.76 between 29 and 32 °C of AT. The acclimation response ratios were ranged between 0.37 and 0.59. Catalase, superoxide dismutase and ATPase activities were increased linearly in liver, gill and kidney, while brain acetylcholine esterase activity decreased linearly with increasing AT. Blood glucose remained unchanged up to AT of 26 °C and increased significantly at AT of 29 and 32 °C. Haemoglobin content was increased linearly with increasing AT. The highest WBC count was observed at 20 °C, and no significant changes found till AT of 26 °C and significantly decreased at 32 °C. Total serum protein and globulin were significantly decreased with increasing AT. Highest values were observed at 20 °C and remained consistent till 26 °C, then decreased significantly. There was no significant change in A/G ratio through the AT 20–29 °C and increased significantly at 32 °C. The increase in CT_max, LT_max and oxygen consumption rate with increasing AT may suggest that the thermal tolerance of T. putitora is dependent on its prior thermal exposure history, and it could adapt to higher AT by altering its haemato-biochemical variables.  相似文献