Retained surgical swabs in dogs     

Papazoglou LG  Patsikas M  Kazakos G  Tsioli V 《The Veterinary record》2011,169(17):450

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Determination of dapsone in muscle tissue and milk using high-performance liquid chromatography-tandem mass spectrometry     

Kaklamanos G  Theodoridis G 《Journal of agricultural and food chemistry》2012,60(1):29-35

A precise and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of dapsone in muscle tissue and milk has been developed. The sample preparation was based on extraction with organic solvent and automated solid-phase extraction (SPE) cleanup. At least three product ions were monitored for the analyte. The method was validated according to the European Decision 2002/657/EC. Estimated analytical limits were 0.0018 ng/g for CCα and 0.0031 ng/g for CCβ in meat and milk. An excellent linear concentration range was observed for both matrices with a correlation coefficient better than 0.997. Recoveries were 105-117% in meat and 101-108% in milk, with satisfactory precision and coefficients of variance (CV) less than 8%. Additionally, a simplified quantification approach was successfully evaluated depending only on the response factor (F) without the use of calibration curve. The developed method provides reliable and sensitive identification and quantification of dapsone in meat and milk.  相似文献   

Temporal changes in the expression of avian β-defensins in the chicken vagina during sexual maturation and Salmonella infection     

Maria Anastasiadou  Melpomeni Avdi  Alexandros Theodoridis  Georgios Michailidis 《Veterinary research communications》2013,37(2):115-122

Avian β-defensins (AvβDs) constitute a family of antimicrobial peptides that are critical to innate immunity in chickens, providing protection against microbial pathogens including Salmonella Enteritidis (SE). As apart from the digestive tract another main route of SE colonization in birds is via infection of the oviduct and specifically of the vagina, the aim of this study was to investigate the expression of the complete family of AvβDs, in the chicken vagina in vivo, to determine whether sexual maturation affects their mRNA abundance and to investigate whether SE infection alters the vaginal AvβDs expression. Expression analysis revealed that 11 members of the AvβD family were expressed in the chicken vagina. Quantitative real-time PCR analysis revealed that the mRNA abundance of five AvβDs was up regulated and of one AvβD was down regulated with respect to sexual maturation. In addition SE infection resulted in a significant induction of AvβD5, 7, 10, 11, 12 and 14 in the vagina of sexually mature birds, and in a significant induction of AvβD5 and 11 in the vagina of aged birds. These findings provide strong evidence to suggest that an AvβD-mediated immune response mechanism exists in the chicken vagina providing protection against bacterial pathogens including Salmonella species.  相似文献   

Association of bulk tank milk urea nitrogen concentration with elevated individual cow values and investigation of sampling frequency for accurate assessment     

Siachos  Nektarios  Panousis  Nikolaos  Valergakis  Georgios E. 《Tropical animal health and production》2019,51(8):2431-2436

Tropical Animal Health and Production - Individual milk urea nitrogen (MUN) levels ≥?19.63 mg/dL have been recently reported to significantly affect fertility. The objectives...  相似文献   

Isolated metal atom geometries as a strategy for selective heterogeneous hydrogenations     

Kyriakou G  Boucher MB  Jewell AD  Lewis EA  Lawton TJ  Baber AE  Tierney HL  Flytzani-Stephanopoulos M  Sykes EC 《Science (New York, N.Y.)》2012,335(6073):1209-1212

Facile dissociation of reactants and weak binding of intermediates are key requirements for efficient and selective catalysis. However, these two variables are intimately linked in a way that does not generally allow the optimization of both properties simultaneously. By using desorption measurements in combination with high-resolution scanning tunneling microscopy, we show that individual, isolated Pd atoms in a Cu surface substantially lower the energy barrier to both hydrogen uptake on and subsequent desorption from the Cu metal surface. This facile hydrogen dissociation at Pd atom sites and weak binding to Cu allow for very selective hydrogenation of styrene and acetylene as compared with pure Cu or Pd metal alone.  相似文献   

Effects of climate and site characteristics on Scots pine growth     

Georgios Xenakis  Duncan Ray  Maurizio Mencuccini 《European Journal of Forest Research》2012,131(2):427-439

Scots pine is a highly diverse species, extended across Europe from Scandinavia to Spain, Italy, Greece and Turkey. It is also a valuable species, used in many commercial monoculture plantations in Great Britain and particularly in Scotland. Because of the diversity of growing environments and its commercial importance, it is necessary to identify the combination of significant factors affecting the observed variability of growth. Temperature, mainly during the growing season, is quite commonly considered as the most important factor in knowledge-based or empirical models. However, in highly oceanic climates like that of Scotland, the impact of temperature may have a less significant impact on growth. Here we argue that other factors, such as incoming winter solar radiation, frost, drought and management also have a significant effect on the growth of Scots pine. In addition, we argue that the already developed Ecological Site Classification knowledge-based model, used as a forest management tool in Great Britain, should be updated to incorporate our findings. Furthermore, we discuss the need to include management impact and possibly more physiological based components in its growth modelling routines, as these would allow the introduction of the effect of winter solar radiation.  相似文献   

Elimination of <Emphasis Type="Italic">Plum pox virus</Emphasis> through in vitro thermotherapy and shoot tip culture compared to conventional heat treatment in apricot cultivar Bebecou     

Georgios C. Koubouris  Varvara I. Maliogka  Kostadinos Efthimiou  Nikolaos I. Katis  Miltiadis D. Vasilakakis 《Journal of General Plant Pathology》2007,73(5):370-373

The effectiveness of in vitro thermotherapy (explants at 35–37°C for 20 days) in obtaining propagating material of apricot (Prunus armeniaca L.) cultivar Bebecou that is free of Plum pox virus (PPV) was compared to a conventional heat treatment technique (potted plants at 30–35°C for 8 weeks). An improved protocol for in vitro thermotherapy was combined with shoot tip culture. The protocol is 5.8 times more effective than conventional thermotherapy while taking half the time and is likely to have a high commercial impact for nurseries.  相似文献   

A survey of tobacco viruses in tobacco crops and native flora in Greece     

Elisavet K. Chatzivassiliou  Konstantinos Efthimiou  Elisseos Drossos  Alexandra Papadopoulou  Georgios Poimenidis  Nikolaos I. Katis 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(10):1011-1023

The most important tobacco producing areas in Greece were surveyed for virus presence, from 1997 to 2000. Tobacco seedlings or plants showing virus-like symptoms were randomly collected from seedbeds or fields, respectively, and tested by ELISA, and/or mechanical inoculation onto indicator plants. Potato virus Y (PVY), Cucumber mosaic virus (CMV) and Tobacco mosaic virus (TMV) were detected in all sampling areas, with TMV mainly found in oriental varieties. Tomato spotted wilt virus (TSWV) consisted a serious endemic virus in Northern Greece (Thrace, Central and Eastern Macedonia), whereas Alfalfa mosaic virus (AMV) was mainly found in regions, where alfalfa was cultivated in the vicinity of tobacco crops. Eggplant mottled dwarf virus (EMDV) was detected in several areas but always in very low incidence (<0.01%). Surveys were also conducted to assess the potential reservoir hosts of PVY, CMV and AMV among weeds collected from highly infected tobacco fields from 1998 to 2000. Among 3450 samples tested for PVY, plants from 17 species in 10 families were found infected. For CMV, 2891 weed samples were tested and 19 species in 12 families were positive. Assays for AMV infection were made on 961 samples and 12 species in 9 families were identified as hosts of this virus.  相似文献   

Sublethal effects of the insecticidal fusion protein ω‐ACTX‐Hv1a/GNA on the parasitoid Eulophus pennicornis via its host Lacanobia oleracea          下载免费PDF全文

Erich YT Nakasu  Filitsa Karamaouna  Georgios K Partsinevelos  Hesham M Abd El Halim  Elaine C Fitches  Prashant Pyati  John A Gatehouse  Angharad MR Gatehouse  Martin G Edwards 《Pest management science》2016,72(3):585-590

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Sonographic evaluation of the uterus in Holstein-Friesian cows without and with disturbances of the puerperium during the first 14 days postpartum     

Tsousis G  Herzog K  Bitter J  Krüger L  Bollwein H 《Berliner und Münchener tier?rztliche Wochenschrift》2008,121(1-2):78-85

The aim of this study was to investigate if, compared with transrectal manual palpation, B-mode sonography provides additional information about the uterine structures and contents of Holstein-Friesian cows without and with disturbances during the early puerperal phase, that lead to a more precise diagnosis. The examinations were carried out once per cow either during the first four days p. p. (contraction phase, KPH) or on days 5 to 10 (desquamation phase, DPH) or on days 11 to 14 (regeneration phase, RPH) in 57 cows with undisturbed (UP) and 69 with disturbed puerperal phases (GP). The mean size of the uterus measured by transrectal palpation was 0.94 to 1.51 points (on a 6-point scale, p < 0.05) larger throughout the examination period and the fluctuation was 1.29 and 0.92 points (on a 4-point scale, p < 0.05) higher in GP cows compared with UP cows during the KPH and RPH, respectively. During the KPH, contractility of the uterus was 0.56 points (on a 3-point scale, p < 0.05) lower in GP cows compared with UP cows. Sonographically the cross sectional area of the most caudally located caruncle was 0.47 to 3.53 cm2 bigger (p < 0.05) in GP cows than in UP cows. The GP cows showed in the KPH a 0.21 cm thinner (p < 0.05) endometrium compared with the UP cows. In the DPH and RPH, the echogenicity was 1.63 and 1.04 points (on a 4-point scale, p < 0.05) higher and the intraluminal diameter of the uterine body was 0.49 to 0.67 cm greater (p < 0.05) in GP cows compared with UP cows, respectively. By means of dicriminant analysis 96.7% and 91.7% of the animals of the GP and UP group, respectively, where classified correctly during the DPH, whereas in the other two phases these proportions were between 70.0% and 87.5%. The results show that B-mode sonography is an additional valuable method for a more accurate differentiation of uterine involutionary processes between cows without and with disturbances of the puerperal phase.  相似文献