Are Circular External Fixators Weakened by the use of Hemispheric Washers?     

Marcellin-Little DJ  Roe SC  Rovesti GL  Bosio A  Ferretti A 《Veterinary surgery : VS》2002,31(4):367-374

OBJECTIVES: To compare the axial mechanical stability of 3 circular external fixators systems with and without hemispheric washers. STUDY DESIGN: Experimental study. METHODS: The axial stiffness and load necessary to produce 0.5 and 1 mm of displacement of 10 circular external fixator constructs from 3 manufacturers were tested on a materials testing machine. The constructs tested included the Small Bone fixator (SBF; Hofmann S.a.S., Monza, Italy), the IMEX ring fixator (IMEX Inc., Longview, TX), and the Multiplanar C-Fix (MCF; PanVet Distribuzione, Seriate, Italy). Five configurations were tested for each construct: (1) conventional nut fixation, (2) hemispheric washer fixation with connecting rods offset by 0, (3) 1, and (4) 2 holes, and (5) with a ring placed at maximum angulation. RESULTS: The loads resisted at 0.5 and 1 mm of displacement did not differ when frame configurations were compared (P =.25733 and.33769, respectively). The linear stiffness of the following configurations were decreasingly stiff: standard constructs, hemispheric washers with connecting rods perpendicular to rings, hemispheric washers with connecting rods offset by 1 hole, hemispheric washers with connecting rods offset by 2 holes, and ring offset in relation to bone model. The SBF constructs tested were 34% and 41% more rigid than the IMEX and MCF constructs tested despite the larger diameter of the connecting rods for the IMEX frames (6 mm) compared with the SBF frames (5 mm). The IMEX constructs tested were 6% more rigid than the MCF constructs tested. CONCLUSIONS: Adding hemispheric washers and angling connecting rods in relation to rings did not influence the loads resisted at 0.5 and 1 mm displacement but decreased construct stiffness. CLINICAL RELEVANCE: The use of hemispheric washers had minor effects on the biomechanical performance of fixator frames tested in this study when used to angle a ring in relation to connecting rods for circular external fixators.  相似文献   

The double-stranded RNA genome of maize rough dwarfFijivirus contains both mono and dicistronic segments     

Cristina Marzachì'  Sara Antoniazzi  Mariangela d'Aquilio  Guido Boccardo 《European journal of plant pathology / European Foundation for Plant Pathology》1996,102(6):601-605

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Equine adipose-tissue derived mesenchymal stem cells and platelet concentrates: their association in vitro and in vivo     

Del Bue M  Riccò S  Ramoni R  Conti V  Gnudi G  Grolli S 《Veterinary research communications》2008,32(Z1):S51-S55

Equine mesenchymal stem cells (MSC) are of particular interest both for basic research and for the therapeutic approach to musculoskeletal diseases in the horse. Their multilineage differentiation potential gives them the capability to contribute to the repair of tendon, ligament and bone damage. MSCs are also considered a promising therapeutic aid in allogeneic cell transplantation, since they show low immunogenicity and immunomodulating functions.Adipose tissue-derived adult equine stem cells (AdMSC) can be isolated, expanded in vitro and then inoculated into the damaged tissue, eventually in the presence of a biological scaffold. Here we report our preliminary experience with adipose-derived mesenchymal stem cells in allogeneic cell-therapy of tendonitis in the horse. MSCs, derived from visceral adipose tissue, were grown in the presence of autologous platelet lysate and characterized for their differentiation and growth potential. Expanded AdMSC were inoculated into the damaged tendon after their dispersion in activated platelet-rich plasma (PRP), a biological scaffold that plays an important role in maintaining cells in defect sites and contributes to tissue healing. Fourteen out of sixteen treated horses showed a functional recovery and were able to return to their normal activity.  相似文献   

Assessment of primers designed for the subspecies-specific discrimination among Babesia canis canis, Babesia canis vogeli and Babesia canis rossi by PCR assay     

Duarte SC  Linhares GF  Romanowsky TN  da Silveira Neto OJ  Borges LM 《Veterinary parasitology》2008,152(1-2):16-20

Canine babesiosis is an infectious disease caused by either Babesia gibsoni or Babesia canis protozoans. The latter is also classified under three different phylogenetic groups, referred to as subspecies B. canis canis, B. canis vogeli and B. canis rossi. The objective of the present study was to validate and standardize a PCR assay to discriminate the organisms at the subspecies level. First, the reference sequences of the 18S rRNA, 5.8S rRNA and 28S rRNA genes, including the internal transcribed spacer 1 (ITS1) and 2 (ITS2) of the most common species and subspecies of the genus Babesia were retrieved from the GenBank database. Subspecies-specific primers (BAB3, BAB4 and BAB5) and one genus-specific primer were designed from the alignment of the sequences. The PCR assays were evaluated in three different combinations of primer pairs in order to assure complete specificity for each reaction. The results of the tests had demonstrated effectiveness of the novel primer pairs BAB1/BAB3, BAB1/BAB4 and BAB1/BAB5 for the amplification of the subspecies-specific target fragments of 746 bp (B. c. canis), 546 bp (B. c. vogeli) and 342 bp (B. c. rossi) by PCR. The original enzymatic amplification assays with novel primers reported in this paper were confirmed to be a reliable tool for the specific discrimination among B. canis subspecies by single-step PCR assays.  相似文献   

Phytochemical Characterization and Effects on Cell Proliferation of Lentisk (Pistacia lentiscus) Berry Oil: a Revalued Source of Phenolics     

Siano  Francesco  Cutignano  Adele  Moccia  Stefania  Russo  Gian Luigi  Volpe  Maria Grazia  Picariello  Gianluca 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(4):487-494

Plant Foods for Human Nutrition - The ethno-pharmaceutical use of the edible fixed oil produced from lentisk (Pistacia lentiscus) berries covers a long tradition in several Mediterranean regions....  相似文献   

Recognition, certification and the combination of recognition and certification: better half evaluated than as a whole disqualified     

van Heeswijk G 《Tijdschrift voor diergeneeskunde》2004,129(23):821-822

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Mucor Species in Orchard Soil – Population Dynamics and Pathogenicity on Pear Fruit     

Marta Mari  Tiziano Cembali  Lucia Casalini  Gian Carlo Pratella 《European journal of plant pathology / European Foundation for Plant Pathology》2000,106(5):449-454

Strains of Mucor piriformis were isolated from orchard soils and from packinghouse dump-tank waters. Pathogen propagules were not found in fruit sample washings. M. piriformis was the most prevalent of the Mucor isolates, only one of 67 isolates was a different Mucor sp., possibly M. racemosus. The population of the pathogen propagules fluctuated in an annual cyclic pattern, declining in warm months and increasing after harvest. The viability of sporangiospores was markedly affected by rain. There was a good correlation (r=0.88) between the number of recovered propagules in the soil and the amount of rainfall. M. piriformis isolates caused decay on pear at 0°C after 14 days.  相似文献   

Naproxen in the horse: Pharmacokinetics and side effects in the elderly     

G. della Rocca  A. Di Salvo  P. Cagnardi  M.C. Marchesi  M.B. Conti 《Research in veterinary science》2014

It is well-known that old animals show physiologic and/or pathologic variation that could modify the pharmacokinetics of drugs and the related pharmacodynamic response. In order to define the most appropriate therapeutic protocol in old horses, pharmacokinetic profile and safety of naproxen were investigated in horses aged over 18 years after oral administration for 5 days at the dose of 10 mg/kg b.w./day. After the first administration, the maximum concentration (C_max 44.21 ± 9.21 μg/mL) was reached at 2.5 ± 0.58 h post-treatment, the harmonic mean terminal half-life was 6.96 ± 1.73 h, AUC_0–24h was 459.71 ± 69.95 h μg/mL, MRT was 7.44 ± 0.74 h and protein binding was 98.47 ± 2.72%. No drug accumulation occurred with repeated administrations. No clinical and laboratory changes were detected after administration of naproxen. Gastric endoscopies performed after the treatment did not show pathological changes of the gastric mucosa.  相似文献   

Ultrastructure of immature and mature human oocytes after cryotop vitrification     

Maria Grazia PALMERINI  Monica ANTINORI  Marta MAIONE  Fabrizio CERUSICO  Caterina VERSACI  Stefania Annarita NOTTOLA  Guido MACCHIARELLI  Mohammad Ali KHALILI  Severino ANTINORI 《The Journal of reproduction and development》2014,60(6):411-420

In vitro maturation of vitrified immature germinal vesicle (GV) oocytes is a promising fertility preservation option. We analyzed the ultrastructure of human GV oocytes after Cryotop vitrification (GVv) and compared it with fresh GV (GVc), fresh mature metaphase II (MIIc) and Cryotop-vitrified mature (MIIv) oocytes. By phase contrast microscopy and light microscopy, the oolemmal and cytoplasmic organization of fresh and vitrified oocytes did not show significant changes. GVv oocytes showed significant ultrastructural alterations of the microvilli in 40% of the samples; small vacuoles and occasional large/isolated vacuoles were abnormally present in the ooplasm periphery of 50% of samples. The ultrastructure of nuclei and mitochondria-vesicle (MV) complexes, as well as the distribution and characteristics of cortical granules (CGs), were comparable with those of GVc oocytes. MIIv oocytes showed an abnormal ultrastructure of microvilli in 30% of the samples and isolated large vacuoles in 70% of the samples. MV complexes were normal, but mitochondria-smooth endoplasmic reticulum aggregates appeared to be of reduced size. CGs were normally located under the oolemma but presented abnormalities in distribution and matrix electron density. In conclusion, Cryotop vitrification preserved main oocyte characteristics in the GV and MII stages, even if peculiar ultrastructural alterations appeared in both stages. This study also showed that the GV stage appears more suitable for vitrification than the MII stage, as indicated by the good ultrastructural preservation of important structures that are present only in immature oocytes, like the nucleus and migrating CGs.  相似文献   

IMAGING DIAGNOSIS—TRANSESOPHAGEAL ULTRASOUND‐GUIDED REMOVAL OF A MIGRATING GRASS AWN FOREIGN BODY IN A DOG     

Domenico Caivano  Antonello Bufalari  Maria Elena Giorgi  Maria Beatrice Conti  Maria Chiara Marchesi  Giovanni Angeli  Francesco Porciello  Francesco Birettoni 《Veterinary radiology & ultrasound》2014,55(5):561-564

A 3‐year‐old English Setter dog was presented for an acute onset of coughing. Tracheobronchoscopic examination allowed localization and removal of one grass awn foreign body. A second migrated grass awn was suspected to be present in the left caudal lung lobe. Transesophageal ultrasound revealed an area of pulmonary consolidation in the dorsomedial portion of left caudal lobe and a linear hyperechoic structure consistent with a grass awn foreign body within the area of consolidation. Transesophageal ultrasonography was also used to provide anatomical landmarks that facilitated successful thoracoscopic removal of the foreign body.  相似文献