Shifts in circulating lymphocyte subsets in cats with feline infectious peritonitis (FIP): pathogenic role and diagnostic relevance     

Paltrinieri S  Ponti W  Comazzi S  Giordano A  Poli G 《Veterinary immunology and immunopathology》2003,96(3-4):141-148

Cats with feline infectious peritonitis (FIP) are usually lymphopenic and have lymphoid depletion evident in spleen and lymph nodes. In particular, the number of CD4+ lymphocytes in tissues decreases during the evolution of FIP lesions. This decrease is most likely due to increased lymphocyte apoptotic rate. In contrast, cats infected with the Feline Coronavirus (FCoV) develop a follicular hyperplasia in the peripheral lymph nodes. The current study was devised to evaluate the possible pathogenic role of shifts in circulating lymphocyte subsets in FIP. Peripheral blood from cats with FIP was evaluated and compared with peripheral blood from clinically healthy cats living in both FCoV-free and FCoV-endemic catteries. Blood from cats with diseases other than FIP was also examined in order to define the diagnostic relevance of the changes. Lymphocyte subsets were analysed by flow cytometry, using a whole blood indirect immunofluorescence technique and mAbs specific for feline CD5, CD4, CD8, CD21. The results of the current study suggest that cats recently infected with FCoV that do not develop the disease have a transient increase in T cells; cats from groups with high prevalence of FIP have a moderate but persistent decrease in T cell subsets; cats with FIP have a very severe decrease in all the subsets of lymphocytes. Moreover, during FIP many lymphocytes do not express any membrane antigen, most likely due to early apoptosis. Cats with diseases other than FIP also had decreased number of lymphocytes: as a consequence, the diagnostic relevance of these findings is very low. Nevertheless, the lack of flow cytometric changes had a high negative predictive value (NPV), thus allowing to exclude FIP from the list of possible diagnoses in cats with normal cytograms.  相似文献   

Feline cutaneous neuroendocrine carcinoma (Merkel cell tumour): clinical and pathological findings     

Bagnasco G  Properzi R  Porto R  Nardini V  Poli A  Abramo F 《Veterinary dermatology》2003,14(2):111-115

A case of a feline Merkel cell tumour is described. An 8-year-old, female cat developed a round, alopecic, reddish mass on the nose. Wide excisional surgery was performed with cartilage resection. Histologically the mass was composed of solid islands of mostly basophilic densely packed cells with a scant cytoplasm, which was suggestive of a neuroendocrine origin. Results of immunohistochemical studies using antibodies against neurone-specific enolase, chromogranin, synaptophysin and pan-cytokeratin allowed classification of the lesion as a Merkel cell tumour. Ultrastructurally, dense core granules were identified in the cytoplasm. In a 2-year follow-up no relapses or metastases were observed. The clinical course recorded is in contrast with the malignant nature of a Merkel cell tumour recently described in a cat and of the human Merkel cell tumour, but is similar to the course of the canine Merkel cell tumour which is often benign. Early diagnosis along with the use of wide surgical excision might be considered an important factor in preventing relapse of this tumour.  相似文献   

Multiplex Nested Reverse Transcription-Polymerase Chain Reaction in a Single Tube for Sensitive and Simultaneous Detection of Four RNA Viruses and Pseudomonas savastanoi pv. savastanoi in Olive Trees     

Bertolini E  Olmos A  López MM  Cambra M 《Phytopathology》2003,93(3):286-292

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The feline dorsal nerve of the penis arises from the deep perineal nerve and not the sensory afferent branch     

Mariano TY  Boger AS  Gustafson KJ 《Anatomia, histologia, embryologia》2008,37(3):166-168

The cat has been used extensively as an animal model for urogenital studies involving the pudendal nerve. However, discrepancies persist in the literature regarding the origin of the dorsal nerve of the penis (DNP). This study used gross dissections and serial histological cross sections to demonstrate that the DNP arises from the deep perineal nerve and not the sensory afferent branch as previously reported. This finding indicates a better than previously appreciated neuroanatomical homology between the cat and human.  相似文献   

Integrated Pest and Disease Control and Pesticide Residues: Results of a Four-year Study     

Marco Trevisan  Ettore Capri  Paola Gobbi  Elena Graviani  Fernando Sicbaldi  Giorgio Mazzali  Giuseppe Reggiani 《Pest management science》1997,50(2):159-164

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Identification of Polyphenolic Compounds in Edible Wild Fruits Grown in the North-West of Italy by Means of HPLC-DAD-ESI HRMS     

Zorzi  Michael  Gai  Francesco  Medana  Claudio  Aigotti  Riccardo  Peiretti  Pier Giorgio 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(3):420-426

Plant Foods for Human Nutrition - The popularity of edible wild fruits has increased in industrialized countries due to their composition and positive effects. The aim of this study has been to...  相似文献   

Effect of Ahasl1‐1 and Ahasl1‐4 alleles on herbicide resistance and its associated dominance in sunflower     

Gabriela Breccia  Laura Gianotto  Emiliano Altieri  Mariano Bulos  Graciela Nestares 《Pest management science》2019,75(4):935-941

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Aspergillus fumigatus population dynamics and sensitivity to demethylation inhibitor fungicides in whole‐crop corn,high moisture corn and wet grain corn silages     

Davide Spadaro  Slavica Matic  Simona Prencipe  Francesco Ferrero  Giorgio Borreani  Ulrich Gisi  Maria Lodovica Gullino 《Pest management science》2020,76(2):685-694

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Immunomodulatory and Antitumoral Activity of Gold Nanoparticles Synthesized by Red Algae Aqueous Extracts     

Noelia Gonzlez-Ballesteros  Lara Diego-Gonzlez  Mariano Lastra-Valdor  Maria Grimaldi  Antonella Cavazza  Franca Bigi  M. Carmen Rodríguez-Argüelles  Rosana Simn-Vzquez 《Marine drugs》2022,20(3)

This study reports on the green and cost-efficient synthesis of gold nanoparticles from three different red algae extracts. The nanoparticles synthesized were fully characterized by UV-Vis spectroscopy, HRTEM, and Z-potential. Relevant components occurring in the extracts, such as polysaccharides or phenolic content, were assessed by analytical techniques such as spectrophotometric assays and liquid chromatography. Finally, the antioxidant, antitumoral, and anti-inflammatory potential of both the extracts and the gold nanoparticles synthesized were analyzed in order to determine a possible synergistic effect on the nanoparticles. The results obtained confirmed the obtainment of gold nanoparticles with significant potential as immunotherapeutic agents. The therapeutic potential of these nanoparticles could be higher than that of inert gold nanoparticles loaded with bioactive molecules since the former would allow for higher accumulation into the targeted tissue.  相似文献   

Zygomatic gland adenoma in a dog: histochemical and immunohistochemical evaluation     

Giudice C  Marco R  Mirko R  Luca M  Giorgio C 《Veterinary ophthalmology》2005,8(1):13-16

Orbital epithelial tumors in dogs are rare and most frequently malignant. Distinguishing their origin from the lacrimal or zygomatic gland is often challenging and is based mostly on tumor location. A case of adenoma involving the orbit in a 13-year-old, female, standard Schnauzer is reported. Histologically, the neoplasm was characterized by nests and cords of epithelial cells mostly forming small glandular structures. The origin of the tumor from the zygomatic gland was determined by histochemical characteristics (alcian blue pH 1 positive staining) of a small remnant of normal gland included within the tumor capsule. The benign nature of our finding was confirmed by follow-up information: 2 years after complete surgical removal of the mass no tumor recurrence or metastases was recorded.  相似文献