Plasmodium vivax and Plasmodium falciparum infection in Neotropical primates in the western Amazon,Brazil     

Tatiene R. M. Silva  Flvia N. L. Barros  Michele Bahia  Francisco D. Sampaio Junior  Sidney S. F. Santos  Larissa S. Inoue  Thamirys S. Gonalves  Laerzio Chiesorin Neto  Diogo C. L. O. Faria  Camila Tochetto  Gisele M. R. Viana  Frederico O. B. Monteiro  Gustavo Ges-Cavalcante  Alessandra Scofield 《Zoonoses and public health》2019,66(7):798-804

The Brazilian Amazon is endemic for malaria and natural infections by Plasmodium spp. have been detected in Neotropical primates. Despite the diversity of primate species in the region, studies on infections by these agents are limited. The aim of the present study was to investigate the frequency of infection by Plasmodium vivax and P. falciparum in free‐born primates that were kept in captivity, in the western Amazon, Brazil. Blood samples were collected from 98 Neotropical primates. Detection of P. vivax and P. falciparum DNA was performed using a semi‐nested PCR, and the amplified products were sequenced. Plasmodium spp. DNA was detected in 6.12% (6/98) of the primates. P. vivax, and P. falciparum DNA was detected in 2.04% (2/98) and 4.08% (4/98) of these mammals, respectively. Sequencing and phylogenetic analysis confirmed the results obtained from the semi‐nested PCR. The presence of infected non‐human primates (NHP) can be auxiliary in the maintenance of P. falciparum and P. vivax and may have implications for the malaria surveillance and control in the Brazilian Amazon. It is necessary to structure an efficient surveillance system for the aetiological agents of malaria that infect NHP and humans to reduce the risk of Plasmodium spp. introduction into new areas, to protect all susceptible species.  相似文献   

Comparison of demographic data,disease severity and response to treatment,between dogs with atopic dermatitis and atopic‐like dermatitis: a retrospective study     

Larissa S. Botoni  Sheila M. F. Torres  Sandra N. Koch  Marcos B. Heinemann  Adriane P. Costa‐Val 《Veterinary dermatology》2019,30(1):10-e4

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Effects of intracameral ascorbic acid on the corneal endothelium of dogs undergoing phacoemulsification          下载免费PDF全文

Ivan R. M. Padua  Gisele P. Valdetaro  Tiago B. Lima  Karina K. Kobashigawa  Paloma E. S. Silva  Marcela Aldrovani  Pedro P. M. Padua  Jose L. Laus 《Veterinary ophthalmology》2018,21(2):151-159

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Reduction in the mRNA expression of sVEGFR1 and sVEGFR2 is associated with the selection of dominant follicle in cows          下载免费PDF全文

PV Ortega Serrano  A Guzmán  CG Hernández–Coronado  H Castillo‐Juárez  AM Rosales‐Torres 《Reproduction in domestic animals》2016,51(6):985-991

The vascular endothelial growth factor (VEGF) is essential for follicular development by promoting follicular angiogenesis, as well as for the proliferation and survival of granulosa cells. The biological effects of VEGF are regulated by two membrane receptors, VEGFR1 and VEGFR2, and two soluble receptors, sVEGFR1 and sVEGFR2, which play an antagonistic role. Thus, the objective of this study was to identify the mRNA expression pattern of total VEGF, VEGFR1, VEGFR2, sVEGFR1 and sVEGFR2 in bovine preselected follicles (PRF) and post‐selected follicles (POF). The mRNA expression of these five genes in both granulosa cells (GC) and theca cells (TC) was compared between follicles classified as PRF and POF based on their diameter and on their ratio of estradiol/progesterone (E2/P4). Results showed a lower expression of mRNA of sVEGFR1 and sVEGFR2 in POF than in PRF (p < .05). Regarding the mRNA expression of total VEGF, VEGFR1 and VEGFR2, there was no difference between POF and PRF follicles (p > .05). Our results showed that the mRNA expression of VEGFR2 and sVEGFR1 was more abundant than the expression of VEGFR1 and sVEGFR2, while GC was the main source of mRNA for total VEGF. On the other hand, TC was the follicular compartment where the receptors were most expressed. Our results suggest that non‐dominant follicles maintain a greater concentration of the mRNA expression of both membrane and soluble VEGF receptors. On the other hand, follicular dominance is related to a reduction in the mRNA expression of sVEGFR1 and sVEGFR2, which may favour VEGF binding with VEGFR2 and, hence, improve the follicular health and development.  相似文献   

Simultaneous application of the vascular endothelial growth factor (VEGF) receptor inhibitor PTK787/ZK 222584 and ionizing radiation does not further reduce the growth of canine oral melanoma xenografts in nude mice     

Inteeworn N  Ohlerth S  Höpfl G  Guscetti F  Bley CR  Wergin MC  Roos M  Gassmann M  Kaser-Hotz B 《Veterinary journal (London, England : 1997)》2007,173(3):564-570

PTK787/ZK 222584 is an inhibitor of the vascular endothelial growth factor (VEGF) receptor tyrosine kinases. In this study, the effectiveness of PTK787/ZK 222584 and radiation on canine oral melanoma xenografts was assessed. Xenografts were treated with radiotherapy (4x6Gy), or with PTK787/ZK 222584, or with a combination of both. Treatment efficacy was assessed using a tumour growth delay assay, serial power Doppler and pO(2) measurements during and after therapy. There was a significant growth delay for the group treated with radiation alone and for the combined treatment group. However, tumour growth delay was similar in both groups. Tumours were hypoxic before irradiation and no significant re-oxygenation occurred during therapy. In all tumours, vascularity and perfusion were significantly lower at the end of the study but no significant differences in perfusion, vascularity and oxygenation status were observed between and within treatment groups. The combination of PTK787/ZK 222584 and radiotherapy did not perform better than radiotherapy alone in this model.  相似文献   

Enhanced resistance to bacterial infection byErwinia carotovora subsp.atroseptica in transgenic potato plants expressing the attacin or the cecropin SB-37 genes     

Patricio Arce  Mauricio Moreno  Mónica Gutierrez  Marlene Gebauer  Paola Dell’Orto  Hebert Torres  Ivette Acuña  Pauline Oliger  Alejandro Venegas  Xavier Jordana  Julio Kalazich  Loreto Holuigue 《American Journal of Potato Research》1999,76(3):169-177

Blackleg and soft rot diseases, caused by the bacteriumErwinia carotovora, are among the diseases that cause important losses in culture and storage of potato. In this paper, we introduced bacterial resistance into potato, via genes encoding for proteins with antibacterial activity. For this purpose, potato clones were transformed either with the gene encoding the acidic attacin protein fromHyalophora cecropia, or with the gene encoding the cecropin analog peptide SB37. These clones were evaluated for soft rot and blackleg resistance, after inoculation with the bacterial strainErwinia carotovora subsp.atroseptica T7. Results reported in this paper indicate that a considerable percentage of the potato clones (15–22%) showed increased resistance to bacterial infection, revealed by reduced severity of blackleg or soft rot symptoms. Expression of the transgenes was demonstrated in some of the clones by Northern blot analysis. This is the first report indicating that expression of the gene encoding for an attacin protein and for the cecropin SB-37 peptide in transgenic potato confers increased resistance to bacterial infection.  相似文献   

Growth and feeding of Patagonian pejerrey Odontesthes hatcheri reared in net cages     

Juan Pablo Hualde  Walter Damián Ceferino Torres  Pablo Moreno  Mirna Ferrada  Mariela Ana Demicheli  Leonardo Javier Molinari  Carlos Marcelo Luquet 《Aquaculture Research》2011,42(6):754-763

An experiment was performed to evaluate the performance of Patagonian pejerrey during net cage rearing in the oligomesotrophic reservoir Exequiel Ramos Mexía. Survival, growth, nutrition and reproduction were evaluated for two lots of pejerrey, initial weights 4.0 and 2.5 g, reared in net cages for 22 and 14 months respectively. Fish were stocked at 29 and 48 individuals m^?3 densities and fed with an experimental pejerrey feed. During the experiment, temperature fluctuated between 6.6 and 19.6 °C. The two lots did not show substantial differences in survival (pooled survival at the end of the experiment >80%) and the thermal‐unit growth coefficient (TGC), daily feed intake and feeding efficiency were 0.43±0.19, 1.70±0.80 and 53.6±9.9 respectively. Digestive tract analysis showed that caged pejerrey can consume substantial quantities of natural food, taking advantage of its planktivorous condition. Pejerrey showed high percentage survival, slow growth and early sexual maturation in captivity. The use of the TGC is proposed as a model for describing the growth pattern of this species and other pejerrey under culture conditions.  相似文献   

Potential role of ectoparasites (Zeuxapta seriolae and Caligus lalandei) in the transmission of pathogenic bacteria in yellowtail kingfish Seriola lalandi,inferred from cultivable microbiota and molecular analyses          下载免费PDF全文

F A Sepúlveda  J F Torres  C D Infante  M T González 《Journal of fish diseases》2017,40(7):979-985

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Comparative fatty acid composition of eggs from wild and captive black sea bass, Centropristis striata L.     

Gloria T Seaborn  Theodore I J Smith  Michael R Denson  Abigail B Walker  & David L Berlinsky 《Aquaculture Research》2009,40(6):656-668

Lipid content, lipid class and fatty acid compositions were determined in eggs from wild and captive black sea bass, Centropristis striata L., from northern (New England) and southern (South Carolina) regions to determine the effects of diet on egg composition and fertilization success. The formulated diets fed to the northern captive (NC) fish were higher in total lipids (22%) compared with the cut fish and squid diet fed to the southern fish (SC; 3.3% lipid) and had a higher relative amount of linoleic acid (LA) and lower relative amounts of docosahexaenoic acid (DHA) and arachidonic acid (AA). These dietary differences were broadly reflected in the egg lipid composition. The LA levels were higher in all lipid classes in eggs of NC fish while AA levels were lower. The DHA was higher in the major polar lipids (PL) (phosphatidylcholine and phosphatidylethanolamine) of SC eggs compared with NC. Compared with wild fish, both captive groups produced eggs with PLs richer in LA and lower in DHA and AA. Over all fish groups, fertilization success was directly correlated with levels of DHA and AA in the PLs and was inversely correlated with LA levels.  相似文献   

The effects of temperature on sex differentiation and growth of black sea bass (Centropristis striata L.)     

Heidi R Colburn  Abigail B Walker  & David L Berlinsky 《Aquaculture Research》2009,40(6):729-736

To examine the effects of temperature on sex differentiation in the black sea bass ( Centropristis striata L.), a protogynous hermaphrodite, juveniles (∼0.5 g) were cultured in recirculating systems at 17, 21 or 25 °C. Growth was assessed at 155, 182, 241 and 275 days post hatch and sex differentiation was determined histologically. No differences were found in the sex ratios of fish reared at different temperatures, but only 55–64% developed as females. Growth was significantly greater in males across all temperature treatments. These results suggest that black sea bass exhibit sexually dimorphic growth patterns and that female-specific sex determination can be disrupted in culture.  相似文献