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121.
122.
Genetic analysis of river,swamp and hybrid buffaloes of north‐east India throw new light on phylogeography of water buffalo (Bubalus bubalis)
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B.P. Mishra P.K. Dubey B. Prakash P. Kathiravan S. Goyal D.K. Sadana G.C. Das R.N. Goswami V. Bhasin B.K. Joshi R.S. Kataria 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2015,132(6):454-466
This study analysed buffaloes from north‐east India and compared their nuclear and mitochondrial DNA variations with buffaloes of mainland India, China, Mediterranean and South‐East Asia. Microsatellite genotypes of 338 buffaloes including 210 from six north‐east Indian buffalo populations and three mainland Indian breeds were analysed to evaluate their genetic structure and evolutionary relationships. Phylogenetic analysis and multidimensional scaling plot of pairwise FST revealed the clustering of all swamp‐type buffaloes of north‐east India with Lower Assamese (significantly hybrid type) buffaloes in one plane and all the mainland river buffaloes in another plane while the upper Assamese buffaloes being distinct from both these clusters. Analysis of mtDNA D‐loop region of 530‐bp length was performed on 345 sequences belonging to 23 buffalo populations from various geographical regions to establish the phylogeography of Indian water buffalo. The swamp buffaloes of north‐east India clustered with both the lineages of Chinese swamp buffalo. Multidimensional scaling display of pairwise FST derived from mitochondrial DNA data showed clustering of upper Assamese, Chilika and Mediterranean buffaloes distinctly from all the other Indian buffalo populations. Median‐joining network analysis further confirmed the distinctness and ancestral nature of these buffaloes. The study revealed north‐east region of India forming part of the wider hybrid zone of water buffalo that may probably extend from north‐east India to South‐East Asia. 相似文献
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AIM: To describe characteristics of Thoroughbred training stables in Matamata and in all other locations in New Zealand combined, over two 19-month time periods in 1996–1997 and 1998–1999, representing equal length periods immediately prior to and after the construction of a new training surface at the Matamata Racing Club. METHODS: Retrospective records covering all horses training and racing in New Zealand during two 19-month time periods (1996–1997 and 1998–1999), covering 161 locations, were obtained from New Zealand Thoroughbred Racing (NZTR). Outcome variables included whether a horse was raced again in the 6 months following any start in the first 13 months of either time period, number of race starts for every horse, and finishing position. Summary measures with confidence intervals (CI) and unadjusted odds ratios (OR), measuring strength of associations for various factors, were computed. RESULTS: The datasets contained information on 45,446 horses, 11,336 races, 5,110 trials and a total of 110,643 race starts. Horses trained at Matamata represented 8% (3,715) of the total horse datasets, and accounted for 11,977 race starts (10.8%). They were more likely to start in a race or trial in either time period and were 1.4 and 1.3 times as likely to finish first, second or third compared with horses trained at other locations in 1996–1997 and 1998–1999, respectively. A 6-month no-race period occurred for 9,306/12,584 (74%) horses that started at least once in the first 13 months of either time period. Horses trained at Matamata were less likely to have a 6-month no-race period than horses trained at other locations in both time periods. There was no effect of time period within each location on the probability of either a horse having a 6-month no-race period or of a race start being followed by a 6-month no-race period, but there was an overall effect of time and more 6-month no-race periods were observed in 1998–1999 relative to 1996–1997. CONCLUSION: Summary statistics are presented for Thoroughbred racing in New Zealand over two 19-month time periods. Differences between the populations of horses trained in Matamata compared with those trained at other locations were attributed, in part, to the fact that many of the more successful racehorse trainers in the country have stables at Matamata. As a result, the population of horses in Matamata may not be representative of the racehorse population in New Zealand. Although more likely to win or place in both time periods, the magnitude of the advantage to horses in Matamata was reduced in 1998–1999 relative to 1996–1997, and this could be due, in part, to effects of the new track surface at Matamata. There was no evidence of a rise in risk of a 6-month no-race period following any race start in those horses trained in Matamata in 1998–1999 relative to either horses trained at other locations or to horses trained in Matamata during the earlier time period. 相似文献
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AIM: To investigate risk factors for injury to the superficial digital flexor tendon (SDFT) and suspensory apparatus (SA) of the forelimbs in Thoroughbred racehorses in New Zealand. METHODS: Poisson and negative binomial regression, with exposure time represented by cumulative training days for each horse, were used to relate explanatory variables to the incidence rate (IR) of cases of inflammation of the SDFT (n=51), and injuries involving the SA (n=48) in a population of 1,571 commercially-trained racehorses over 554,745 study days. Only the first occurrence of an injury for any one horse was eligible for inclusion. Separate analyses were run for data from horses in training regardless of whether they had started in a trial or race, and using a subset of these data restricted to those preparations associated with at least one start in a trial or race. Results were reported as incidence rate ratios (IRR) and 95% confidence intervals (CI). RESULTS: Male horses had a higher risk of injury to the SA (IRR 2.57; p=0.005) and tended to have a higher risk of injury to the SDFT (IRR 1.74; p=0.09) than female horses. Increasing age was associated with increased risk of injury. Horses aged 4 and ≥5 years were 6.76 (p<0.001) and 15.26 (p<0.001) times more likely to incur injury to the SDFT, and 2.91 (p=0.02) and 3.54 (p=0.005) times more likely to incur injury to the SA, respectively, than 2-year-olds. Horses were more likely to suffer an injury to the SDFT or SA in a training preparation that was not associated with any starts in official trials or races compared with those preparations that were associated with more than one start (p<0.001), and more likely to injure the SA compared with preparations containing one start (p=0.03). The IR of injury to the SDFT tended to be lower between November–January (IRR 0.78; p=0.08) and February-April (IRR 0.75; p=0.08) compared with August–October. Incidence of injury to the SDFT or SA was not associated with the cumulative distance raced in the last 30 days of a training preparation. CONCLUSION: This study identified risk factors for injury to the SDFT and SA in Thoroughbred racehorses in New Zealand. Injuries were more likely in males, older horses and in horses in training preparations without any starts. There was no evidence of association between injury and cumulative high-speed exercise. 相似文献
126.
CASE HISTORY: Twelve of 150 goat kids, 4–10 days old, died 3 days after disbudding with a hot iron. Another 18 kids had been ill the previous day but survived following antibiotic therapy. Five of the dead kids were necropsied. PATHOLOGICAL FINDINGS: There was necrosis and haemorrhage of the skin, subcutaneous tissues and frontal bone at disbudding sites in all five kids examined post mortem. Beneath disbudding sites in 4/5 kids there were bilateral, dark red, often cavitated areas of necrosis extending deep into the frontal cortex of the brain. Histologically, these areas consisted of coagulation necrosis, haemorrhage, vascular thrombosis and suppurative inflammation. Numerous bacteria, predominantly large Grampositive rods, were present in the necrotic brain tissue. In the remaining kid, bilateral areas of yellow discolouration and flattening of gyri in frontal lobes corresponded histologically to extensive polioencephalomalacia. A mixed growth of aerobes and anaerobes was cultured from the brain of one kid with suppurative lesions. CLINICAL RELEVANCE: Thermal disbudding of neonatal kids is widely practised in dairy goat herds and is considered the method of choice for disbudding in New Zealand. However, the skull of goat kids is much thinner than that of calves and the safety margin for thermal injury to the brain is markedly reduced. This report highlights the risks associated with the technique and its potential as a welfare issue. 相似文献
127.
CASE HISTORY: Investigations were conducted to determine the cause of an acute, multi-farm outbreak of porcine respiratory disease that included diarrhoea and subsequent loss of body condition in affected pigs. A definition for post-weaning multisystemic wasting syndrome (PMWS) including both clinical and pathological features, previously developed for the pig industry in New Zealand, was applied to the current outbreak. In addition to self-reporting by owners of affected farms, local veterinarians, disease and epidemiology consultants, and animal health officials from the Ministry of Agriculture and Forestry (MAF) were involved in conducting farm visits and submission of diagnostic specimens. CLINICAL FINDINGS AND DIAGNOSIS: Pathogens known to be endemic in the pig industry in New Zealand as well as likely exotic diseases were excluded as causative agents of the outbreak. Clinical signs including dyspnoea, diarrhoea, and rapid loss of body condition were consistent with the New Zealand case definition for PMWS. Interstitial pneumonia, pulmonary oedema, generalised lymph-node enlargement, and presence of porcine circovirus type 2 (PCV2) inclusion bodies were consistently identified in affected pigs. Classical swine fever virus (CSFv), Porcine reproductive and respiratory syndrome virus (PRRSv), and Influenza virus were ruled out, using molecular and traditional virological techniques. Spread of the disease between farms was hypothesised to be facilitated by locally migrating flocks of black-backed seagulls. The original source of the disease incursion was not identified. DIAGNOSIS: Based on the consistent presence of circovirusassociated lesions in lymphoid tissues in combination with generalised enlargement of lymph nodes, histiocytic interstitial pneumonia, clinical wasting, and poor response to antibiotic therapy, a diagnosis of PMWS was made. CLINICAL RELEVANCE: PMWS should be considered in the differential diagnoses of sudden onset of respiratory dyspnoea, diarrhoea, and rapid loss of body condition in young pigs in New Zealand pig herds. 相似文献
128.
Hopker Andy Pandey Naveen Saikia Dibyajyoti Goswami Jadumoni Hopker Sophie Saikia Roopam Sargison Neil 《Tropical animal health and production》2019,51(4):819-829
Tropical Animal Health and Production - During September and October 2017, a highly fatal outbreak of a disease clinically indistinguishable from goat pox occurred in the villages around the... 相似文献
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130.
Aithal HP Kinjavdekar P Amarpal Pawde AM Singh GR Pattanaik AK Varshney VP Goswami TK Setia HC 《Veterinary research communications》2009,33(4):331-343
The study was undertaken to find out the effects of over supplementation of dietary calcium and vitamin D3 on the mineralization
of growing skeleton, taking rabbit as an animal model; further to study the effects of Nandrolone deconoate and TGF-β1 on
the mineralization of osteopenic bones. Twenty four New Zealand White rabbits of either sex, 60 day old, were randomly divided
in 4 equal groups, A, B, C and D. The animals of groups B, C and D were administered with oral supplementation of calcium
(2000 mg/kg of standard rabbit feed) and vit-D3 (1000 IU/kg of standard feed) for 60 days. The animals of group A were given
standard ration without any supplementation. After 60 days, the Ca-vit.D3 supplementation was discontinued; and the animals
of group C were administered with TGF-β1 (10 ng, i.m.) once in every three days and animals of group D were given Nandrolone
deconoate (10 mg, i.m.) once every week for 30 days, whereas in animals of group B, no treatment was given. All the animals
were evaluated based on different observations like body weight, radiographic observations, circulating biochemical and hormone
profile (plasma Ca, IP, AP, OC and iPTH) every 15 days up to 60 days after initiation of treatment. The results indicated
that the body weight of rabbits in different groups increased gradually and steadily at different intervals till the end of
observation period, however, the increase was non-significantly more in group D. The CI in group A increased gradually at
different intervals; whereas in groups B, C and D, there was no appreciable increase in the CI during the period of Ca-vit.D3
supplementation, suggesting development of osteopenia. Treatment with TGF-β1 did not increase the CI significantly, whereas
Nandrolone treatment resulted in significant increase in the CI on days 45 and 60. The plasma Ca levels showed slight but
gradual increase from day 0 to 60 in almost all groups. Subsequently also, there was no marked change at different intervals
in groups A and B; however, significant reduction in plasma Ca was noticed in group C on 15th day and in group D on 60th day after initiation of treatment. Plasma IP levels in groups B and C showed a decreasing trend up to day 60. After discontinuation
of Ca-vit.D3 supplementation, in group B, it further decreased to remain significantly lower on 15th day, and in groups C and D, it increased significantly on 60th post-treatment day. There was no significant change in the AP activity during the entire period of study in group A; whereas
significant reduction in AP activity was measured on 30th and 60th days of treatment in groups B and C, and on 15th day of treatment in group D. Plasma iPTH values did not show any significant change at any interval during the first 60 days
in all groups. On 30th and 60th days of treatment, the mean iPTH level remained significantly lesser in group B. In all groups treated with over supplementation
of Ca and vit.D3, there was a non-significant increase in the plasma OC levels up to day 60; however, there was no significant
difference between the groups. It can be concluded that additional supplementation of Ca and vit.D3 results in osteopenia
in growing rabbits. Administration of Nandrolone helps to increase the mineral density in osteopenic bones, whereas TGF-β1
does not seem to have positive effect on the skeletal mineralization. 相似文献