全文获取类型
收费全文 | 81455篇 |
免费 | 6809篇 |
国内免费 | 8474篇 |
专业分类
林业 | 9480篇 |
农学 | 13306篇 |
基础科学 | 7021篇 |
14143篇 | |
综合类 | 21874篇 |
农作物 | 3903篇 |
水产渔业 | 4645篇 |
畜牧兽医 | 13659篇 |
园艺 | 2537篇 |
植物保护 | 6170篇 |
出版年
2024年 | 132篇 |
2023年 | 678篇 |
2022年 | 1834篇 |
2021年 | 2882篇 |
2020年 | 3068篇 |
2019年 | 3429篇 |
2018年 | 2620篇 |
2017年 | 3425篇 |
2016年 | 3423篇 |
2015年 | 4033篇 |
2014年 | 3846篇 |
2013年 | 4864篇 |
2012年 | 5071篇 |
2011年 | 5477篇 |
2010年 | 5357篇 |
2009年 | 4617篇 |
2008年 | 4344篇 |
2007年 | 4677篇 |
2006年 | 4341篇 |
2005年 | 4031篇 |
2004年 | 1943篇 |
2003年 | 1836篇 |
2002年 | 1659篇 |
2001年 | 1685篇 |
2000年 | 1973篇 |
1999年 | 2118篇 |
1998年 | 1978篇 |
1997年 | 1684篇 |
1996年 | 1510篇 |
1995年 | 1460篇 |
1994年 | 1288篇 |
1993年 | 1206篇 |
1992年 | 1011篇 |
1991年 | 785篇 |
1990年 | 694篇 |
1989年 | 520篇 |
1988年 | 423篇 |
1987年 | 291篇 |
1986年 | 150篇 |
1985年 | 71篇 |
1984年 | 58篇 |
1983年 | 58篇 |
1982年 | 44篇 |
1981年 | 52篇 |
1980年 | 31篇 |
1979年 | 12篇 |
1978年 | 8篇 |
1977年 | 6篇 |
1965年 | 6篇 |
1964年 | 5篇 |
排序方式: 共有10000条查询结果,搜索用时 828 毫秒
991.
高地隙喷雾机驱动轮独立式驱动系统转向控制模型研究 总被引:1,自引:0,他引:1
车辆转弯时的内外轮速平衡问题是车辆驱动轮独立式驱动技术研究的关键问题。根据车辆转弯时轮速不等的现象,以阿克曼转向原理为基础,对车辆转向模型进行简化,运用运动学分析原理研究了内外轮速与转弯时车速及转向角之间的关系;结合车辆动力学原理建立车辆转弯临界模型,分析了车辆安全转弯临界状态,最后设计了实际应用方案。选择在工作原理和安全控制方面较普通的电控差速器更为简单易行,提高了车辆转向性能、简化了车辆结构、降低了成本。此技术运用到农田高地隙喷雾机上可简化其结构、减轻质量,且在农田行走更灵活方便。 相似文献
992.
993.
994.
995.
996.
The experiment was conducted to discuss the difference of binding time of green fluorescent protein B.melitensis M5 (GFP-M5) and B.abortus S19 (GFP-S19) infecting the mouse macrophagocyte (RAW264.7),lysosome,endoplasmic reticulum and golgi body in the initial stage and compare the binding rate of GFP-M5,GFP-S19 with organelle in different timeline,respectively,by confocal laser scanning microscope (CLSM) and flow cytometry.The result showed that GFP-M5 and GFP-S19 were successfully constructed.The intracellular survival ability of Brucella M5,Brucella S19,GFP-M5 and GFP-S19 were not obvisouly affected after infecting RAW264.7.GFP-M5 and GFP-S19 could enter the macrophagocyte in 30 mins,and in 2 h the Brucella could reach lysosome,endoplasmic reticulum and golgi body.In addition,the binding time for two attenuated vaccine did not show differences in 1,2,3 and 4 h.The content of GFP+ cell produced by RAW264.7 infected by GFP-M5 and GFP-S19 did not show significant differences (P>0.05).Therefore,the two strains did not have significant differences in the invasion ability in the initial stage of infecting host cell. 相似文献
997.
XU Wan-yun WANG Hui-min WANG Wen-lun HU Meng-wei YAN Guo LI Jian-hua GAO Jian-feng 《中国畜牧兽医》2016,43(3):568-576
The specific primers were designed according to Ovis aries DRA gene sequence deposited in GenBank and the multiple cloning site of the plasmid pYD1,which was a vector used for protein surface display on Saccharomyces cerevisiae.The gene encoding DRA was amplified by PCR using the genomic RNA of Ovis aries.The 762 bp fragment was cloned and released in GenBank and registration number was KR422362.The PCR product was inserted into the yeast surface display plasmid vector pYD1 by double enzyme digestion.It was indicated that DRA gene was successfully integrated into the genome.Dot mutation was made at both ends of exon 2 in DRA gene for making restriction enzyme cutting site and design the exon 2 specific primers according to mutated Ovis aries DRA gene sequence.Sequenced exon 2 amplification products based on DNA pooling of sheep large sample template was analyzed the polymorphic loci.The polymorphic exon 2 246 bp fragment was obtained by double enzyme digestion and connected to surface display restructuring mutation carriers pYD1-DRA by the same double enzyme digestion,and then we successfully constructed yeast surface display libraries.We transformed it into Saccharomyces cerevisiae EBY100 cell.Yeast monoclone was identified by PCR amplification and sequencing,and we confirmed that DRA gene had been integrated into Saccharomyces cerevisiae genome.After galactose induced,it was detected that DRA gene library had been successfully demonstrated on the yeast cell surface under the fluorescence microscope by immunofluorescence method. 相似文献
998.
Immunogenic properties of Landrace pigs selected for resistance to mycoplasma pneumonia of swine
下载免费PDF全文
![点击此处可从《Animal Science Journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Liushiqi Borjigin Tomoyuki Shimazu Yuki Katayama Meihua Li Takumi Satoh Kouichi Watanabe Haruki Kitazawa Sang‐gun Roh Hisashi Aso Kazuo Katoh Takafumi Uchida Yoshihito Suda Akiko Sakuma Mituru Nakajo Keiichi Suzuki 《Animal Science Journal》2016,87(3):321-329
Mycoplasma pneumonia of swine (MPS) lung lesions and immunogenic properties were compared between a Landrace line that was genetically selected for reduced incidence of pulmonary MPS lesions, and a non‐selected Landrace line. The MPS‐selected Landrace line showed significantly lower degrees of pulmonary MPS lesions compared with the non‐selected Landrace line. When changes in immunity before and after vaccination were compared, the percentage of B cells in the peripheral blood of the MPS‐selected Landrace line was significantly lower than that of the non‐selected line. Furthermore, the concentration of growth hormone and the mitogen activity of peripheral blood mononuclear cells in the MPS‐selected Landrace line showed significantly (P < 0.05) lower increases after vaccination than the non‐selected line. Conversely, the concentration of peripheral blood interferon (IFN)‐γ and salivary immunoglobulin A (IgA) after Mycoplasma hyopneumoniae vaccination was significantly higher in the MPS‐selected Landrace line than in the non‐selected line. Gene expression of toll‐like receptor (TLR)2 and TLR4 was significantly higher in the MPS‐selected Landrace line in immune tissues, with the exception of the hilar lymph nodes. The present results suggest that peripheral blood IFN‐γ, salivary IgA TLR2, and TLR4 are important immunological factors influencing the development of MPS lesions. 相似文献
999.
The effect of lactic acid bacterial starter culture and chemical additives on wilted rice straw silage
下载免费PDF全文
![点击此处可从《Animal Science Journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Yan‐Su Wang Wei Shi Lin‐Ting Huang Cheng‐Long Ding Chuan‐Chao Dai 《Animal Science Journal》2016,87(4):525-535
Lactic acid bacteria (LAB) are suitable for rice straw silage fermentation, but have been studied rarely, and rice straw as raw material for ensiling is difficult because of its disadvantages, such as low nutrition for microbial activities and low abundances of natural populations of LAB. So we investigated the effect of application of LAB and chemical additives on the fermentation quality and microbial community of wilted rice straw silage. Treatment with chemical additives increased the concentrations of crude protein (CP), water soluble carbohydrate (WSC), acetic acid and lactic acid, reduced the concentrations of acid detergent fiber (ADF) and neutral detergent fiber (NDF), but did not effectively inhibit the growth of spoilage organisms. Inoculation with LABs did not improve the nutritional value of the silage because of poor growth of LABs in wilted rice straw. Inoculation with LAB and addition of chemical materials improved the quality of silage similar to the effects of addition of chemical materials alone. Growth of aerobic and facultatively anaerobic bacteria was inhibited by this mixed treatment and the LAB gradually dominated the microbial community. In summary, the fermentation quality of wilted rice straw silage had improved by addition of LAB and chemical materials. 相似文献
1000.
Effects of dietary supplementation of emulsifier and carbohydrase on the growth performance,serum cholesterol and breast meat fatty acids profile of broiler chickens
下载免费PDF全文
![点击此处可从《Animal Science Journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
A total of 540 2‐day‐old male Ross 308 broilers were used in a 35‐day experiment and were randomly divided into five treatments: (i) NC (low energy); (ii) PC (high energy diet); (iii) P1 (NC + 0.1% carbohydrases); (iv) P2 (NC + 0.05% emulsifier); and (v) P3 (NC + 0.1% carbohydrases + 0.05% emulsifier). From days 0 to 21, body weight gain in PC and P3 treatments increased (P < 0.05) compared with NC treatment. The chicks fed PC, P1, P2 and P3 improved (P < 0.05) feed conversion ratio compared with the NC treatment throughout the whole experiment. Abdominal fat weight was heavier (P < 0.05) in PC, P2 and P3 treatments than in NC and P1 treatments. On day 35, serum total cholesterol and low density lipoprotein cholesterol concentration were higher (P < 0.05) and high‐density lipoprotein cholesterol was lower (P < 0.05) in NC and P2 treatments than in PC, P1 and P3 treatments. The concentration of oleic acid, linoleic acid and total unsaturated fatty acids were highest (P < 0.05) in PC than in the other treatments. In conclusion, the results indicate that low energy density diet had lower growth performance, while the inclusion of emulsifier and carbohydrases in low energy diets can partially improve growth performance. 相似文献