山楂叶螨抗药性及混配增效作用   总被引：4，自引：2，他引：4  

刘金香  胡美英  韩巨才  刘慧平  钟国华 《植物保护学报》2004,31(2):199-204

采用载玻片浸渍法测定了山西省忻州、晋中、运城地区山楂叶螨种群对9种杀螨剂的抗药性水平.测定结果表明:忻州地区种群对甲氰菊酯和三氯杀螨醇表现中抗,抗性指数FR分别为17.79和31.93倍,对水胺硫磷和毒死蜱表现低抗,FR值分别为7.74和5.35倍;晋中地区种群对水胺硫磷、甲氰菊酯和三氯杀螨醇均表现中抗,FR值为22.42～33.00倍,而运城地区种群对这3种药剂产生了高抗,FR值分别为135.05、41.53和1714.01倍;运城地区种群对毒死蜱表现中抗,FR值为34.49倍;晋中和运城地区种群对双甲脒表现低抗,FR值分别为5.15和6.89倍;3个种群对阿维菌素、苯丁锡、三唑锡和四螨嗪仍相对敏感.混配增效作用测定结果表明,阿维菌素与甲氰菊酯1:20、1:10、1:5组合以及阿维菌素与三氯杀螨醇1:20、1:10组合均具有明显增效作用,共毒系数为189.63～363.30,其中阿维菌素与甲氰菊酯1:20和1:10组合以及阿维菌素与三氯杀螨醇1:20组合是比较理想的混配组合.  相似文献   

菜豆新品种将军油豆的选育   总被引：1，自引：1，他引：1  

冯国军  曲敏  刘大军  闫田柱  王玉莉  叶永亮 《中国蔬菜》2004,1(5):22-23

将军油豆是以农家品种大马掌为母本 ,家雀蛋为父本人工杂交后系统选育而成。中熟 ,蔓生 ,从播种到采收 6 5d(天 ) ,生长势强 ,嫩荚绿色 ,扁条形 ,荚尖部有红条纹 ,平均荚长 2 0cm ,荚宽 2 .4cm ,单荚质量 2 4g ,纤维少 ,肉质面 ,属典型的东北优质油豆角类型。抗炭疽病、锈病 ,适应性广 ,春秋皆可种植 ,露地、保护地兼用  相似文献   

Analysis of blood biochemistry and arterial blood gas of systemic inflammatory response syndrome in the early time after trauma     

ZHOU Ze-qiang  YANG Feng  HAN Qing 《园艺学报》2004,20(9):1716-1718

AIM: To explore the variation of blood biochemistry and arterial blood gas of patients with systemic inflammatory response syndrome (SIRS) in the early time after trauma and improve the diagnosis and first aid. METHODS: Eighty-eight patients with trauma from August 2003 to February 2004 were divided into two groups by their AIS-ISS90 score. The data of temperature, pulse, respiratory rate, white blood cell counts, Hb, blood glucose and arterial blood gas (PaO₂, PaCO₂, HCO₃^-, AG) were collected and compared with each group by statistic methods. RESULTS: Of the 88 patients, 49 underwent SIRS, 12 in light trauma group (ISS≥16) and 37 in severe trauma group (ISS<16). Compared with light trauma group, the data of pulse, respiratory rate, white blood cell counts, blood glucose, AG and rate of SIRS of severe trauma group were higher, PaO₂ and HCO₃^- were lower and the cases of PaCO₂>45 mmHg or <35 mmHg were more (P<0.01). The data of temperature and Hb had not significant difference between two groups (P>0.05). 13 patients had MODS in severe trauma group and 2 died while none had MODS or died in light trauma group. CONCLUSION: Application of AIS-ISS90 and SIRS-related blood biochemistry and arterial blood gas is beneficial for the diagnosis and treatment of patients in the early time after trauma.  相似文献   

Inhibition of K562 cell growth by antisense drug targeting with VEGF mRNA in vitro     

FEI Jia  ZHANG Huan  HAN Zhong-chao 《园艺学报》2004,20(10):1769-1772

AIM: To investigate inhibition of K562 cell growth by antisense drug targeted VEGF mRNA. METHODS: X7, 20-mer antisense sequences were selected, synthesized and modified with phosphorothioate. The drug was transfected into K562 cells in the present of lipofection. Cell growth was assayed by trypan blue dye exclusion assay and MTT. The level of VEGF protein in the media was determined by ELISA. The morphology of apoptotic cells were observed by Giemsa staining, and the propotion of apoptotic cells was detected by flow cytometry. RESULTS: The antisense drug inhibited growth of K562 and downregulated expression of VEGF protein significantly, compared with Scrambed control group and showed dose-dependent relation. Signs of apoptosis of K562 cells were not observed. CONCLUSION: Inhibition of K562 cell proliferation, but not cells apoptosis induction is the mechanism of inhibing growth of K562 cells by antisense drug targeted VEGF mRNA. At same time, VEGF has function of promoting K562 cell proliferation, and VEGF mRNA may be a new target attached by drugs.  相似文献   

薇甘菊天敌安婀珍蝶生物学特性的研究     

刘雪凌  韩诗畴曾玲李丽英  余金咏 《昆虫天敌》2004,26(3):108-112

安婀珍蝶Actinote anteas(Doubleday ＆ Hewitson)是薇甘菊的天敌,取食薇甘菊Mikania micrantha Kunth.的叶片,能有效的控制薇甘菊的蔓延和生长。在广州市和深圳龙岗进行的观察表明,在实验室条件下,安婀珍蝶一年生长3代~4代。世代平均历期为112.44±1.18天;卵期平均为11.44±1.18天;幼虫期平均为82.54±4.05天;蛹期平均为12.98±1.31天;成虫期平均为7.33±0.80天。本文还记述了该蝶各虫态的形态特征、生活习性及其天敌等。  相似文献   

引诱剂在综合防治桔小实蝇作用中的评估   总被引：5，自引：1，他引：5  

徐洁莲欧剑峰  韩诗畴黄鸿 吴华 《昆虫天敌》2004,26(4):145-149

以使用引诱剂为主 ,坚持在杨桃园挂引诱笼 2 - 3年后 ,大量降低田间桔小实蝇BactroceradorsalisHendel雄虫数量和卵的受精率 ,配合其它的综防措施 ,防效逐年提高。三个综防点试验实践表明 :杨桃每果虫口数 ,卵孵化率及受害果率大大降低 ,防效达 92 5%。对桔小实蝇的防治初见成效。  相似文献   

Effects of inhibition of Kupffer cell and splenectomy on thioacetamide-induced hepatic injury     

ZHAO Yan  LIU Jin-chun  HAN De-wu  XU Rui-ling 《园艺学报》2004,20(11):2076-2078

AIM: To examine the effects of inhibition of Kupffer cell and splenectomy on intestinal endotoxemia and hepatic injury. METHODS: The hepatic injury model was established by treatment with thioacetamide (TAA). At the same time, inhibition of Kupffer cells by intravenous GdCl₃ and splenectomy were performed. Serum alanine aminotransferase (ALT), TNF-α, endotoxin content and phagocytic index were observed. RESULTS: In the TAA+GdCl₃ group, and TAA+splenectomy group, the endotoxin content was significently higher than that in normal and TAA group (P<0.05). The plasma TNF-α, ALT and total bilirubin were significantly lower than those in TAA group (P<0.05). CONCLUSION: Inhibition of Kupffer cells and splenectomy increase plasma endotoxin level, but decreases the plasma TNF-α levels and alleviates the hepatic injury induced by TAA.  相似文献   

秀丽纤杆线虫在寄生性线虫抗原研究中的应用     

韩春来  汪明 《中国兽医寄生虫病》2004,12(1):33-36

秀丽纤杆线虫 (Caenorhabditis elegans)是一种模式线虫 ,目前广泛应用于寄生性线虫基因的功能研究 ,并且还被用于捻转血矛线虫等寄生性线虫疫苗侯选抗原基因的表达和耐药性产生机制方面的研究  相似文献   

体外培养条件对延边黄牛受精卵体外发育的影响     

罗光彬  韩雪花  金花子  蔡春化  金庆国 《中国兽医学报》2004,24(2):192-194

从延边黄牛卵巢中采集未成熟的卵母细胞进行体外成熟培养、体外受精及受精卵体外培养。结果表明 :1将卵子用 2种不同的培养液进行体外成熟培养和受精卵体外培养 ,TCM199组的卵裂率 (5 6 .3% )极显著高于 D- PBS组(33.6 % ) (P<0 .0 1) ;TCM199组的囊胚发育率和孵化率 (15 .1%、13.7% )虽高于 D- PBS组 (10 .5 %、8.7% ) ,但 2个组之间无显著差异 (P>0 .0 5 )。2以 TCM199作为基础培养液 ,分别用含激素培养液和不含激素培养液进行成熟培养和受精卵体外培养 ,添加激素组的卵裂率、囊胚发育率及囊胚孵化率 (81.2 %、17.5 %、15 .3% )高于没有添加激素的对照组 (75 .8%、12 .1%、10 .5 % ) ,但 2个组之间无显著差异 (P>0 .0 5 )。 3体外受精卵与单层颗粒细胞共培养组的卵裂率、囊胚发育率及囊胚孵化率 (78.0 %、11.5 %、9.9% )显著高于非共培养组 (6 8.1%、5 .4 %、3.6 % ) (P<0 .0 5 )。  相似文献   

基于GIS的丹东地区柞蚕农业气候区划   总被引：2，自引：0，他引：2  

纪瑞鹏  张玉书  韩卫东 《蚕桑通报》2004,35(2):27-30

根据当地柞蚕实际生产与气象条件的关系,确定了柞蚕生产的农业气候区划指标.运用丹东地区1:250000地形数据和丹东及周边地区气象站30年的气候资料,建立区划指标小网格推算模型,应用GIS技术对丹东地区柞蚕生产进行气候区划,将该区划分为气候最适宜区、气候适宜区、气候次适宜区和气候不适宜区,为丹东柞蚕生产的合理布局和发展提供参考依据.  相似文献