Direct plant regeneration from mature leaf explants of pistachio, Pistacia vera L.     

Engin Tilkat  Ahmet Onay  Hakan Yıldırım  Emine Ayaz 《Scientia Horticulturae》2009

A protocol was developed for direct shoot and plantlet regeneration from in vitro regenerated leaf explants of male Pistacia vera L. cv. ‘Atl?’. Leaves excised from axenic shoot cultures of pistachio were used to induce organogenesis on a Murashige and Skoog (MS) medium with Gamborg vitamins supplemented with combinations of different concentrations of BAP and IAA. The highest adventitious shoot regeneration in 35% of the explants, with the number of shoots ranging from 2 to 3 per explant, occurred in the explants cultured during the establishment phase in the medium with 1 mg l⁻¹ IAA and 2 mg l⁻¹ BAP. For shoot multiplication, the highest number of new microshoot/explants (5.76) was obtained in a culture medium supplemented with 1 mg l⁻¹ BAP, but it was not significantly different from the number obtained at 2 mg l⁻¹ BAP. A high rooting frequency (84%) for microshoots was recorded on a medium supplemented with 2 mg l⁻¹ IBA. In vitro rooted plantlets were transferred to pots filled with a mixture of soil, sand and peat (1:1:1). They were weaned in a growth room and finally moved to a greenhouse. This protocol could be utilized for in vitro clonal propagation of this economically important plant.  相似文献   

Neospora caninum associated with epidemic abortions in dairy cattle: The first clinical neosporosis report in Turkey     

Oğuz Kul  Nalan Kabakci  Kader Yildiz  Naci Öcal  Hakan Kalender  N. Aycan İlkme 《Veterinary parasitology》2009

Neospora caninum, a protozoan parasite, has been considered as one of the most important etiological agents responsible for abortion in dairy cattle throughout the world since it was first identified in dogs in 1988. In this report, characteristics of neosporosis, detected in a dairy cow ranch having epidemic abortions as high as 18.4%, were described. Blood samples were collected from 25 infertile or aborted dairy cattle, 6 calves born in 2006 and 40 heifers that were born in 2005 and raised in the same ranch. Necropsy was conducted in a 20-day-old Simmental calf that exhibited neurological signs including incoordination, head shaking, hyperextension in forelimbs and hindlimbs and tremor. The seroprevalance in aborted or infertile dairy cattle, heifers, and calves was 60%, 40%, and 33.3%, respectively. The mothers of seropositive two calves including clinically affected calf and its dam were N. caninum seropositive. In immunoperoxidase examinations, N. caninum antigen immunopositivity was observed in the degenerative and necrotic neurons in the brain, cerebellum as well as neurons in dorsal root ganglia of the cervical and thoracic regions of the spinal cord. In the heart, myocytes and Purkinje cells exhibited granular and linear patterns of immunoreactivity. Striated myofibers around the eyeball also showed immunolocalization for N. caninum antigen. Ultrastructurally, tachyzoites with typical apical complex, rhoptries and double-layered parasitic membrane were detected in the brain and heart sections. In conclusion, this report described clinical neosporosis for the first time in Turkey with tissue localization of the causative agents. This scientific communication also discusses the possible impact of cattle neosporosis by clinical, serologic and pathologic evidences collected from the survey of calves born in two successive generations in a ranch.  相似文献   

Robust molecular detection of the new Tomato brown rugose fruit virus in infected tomato and pepper plants from Turkey          下载免费PDF全文

Hakan FIDAN  Pelin SARIKAYA  Kubra YILDIZ  Bengi TOPKAYA  Gozde ERKIS  Ozer CALIS 《农业科学学报》2021,20(8):2170-2179

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EFFECT OF CITRUS ROOTSTOCKS ON LEAF MINERAL COMPOSITION OF ‘OKITSU’, ‘CLAUSELLINA’, AND ‘SILVERHILL’ MANDARIN CULTIVARS     

Celil Toplu  Veli Uygur  Mustafa Kaplankıran  T. Hakan Demirkeser  Ercan Yıldız 《Journal of plant nutrition》2013,36(9):1329-1340

Citrus performance is strongly related with rootstock. This study was conducted to investigate leaf nutrient contents of ‘Okitsu’, ‘Clausellina’ and ‘Silverhill’ mandarin cultivars budded onto sour orange, ‘Carrizo’ and ‘Troyer’ citrange rootstocks in Dörtyol, Turkey in 2004 and 2005. The maximum nitrogen (N), potassium (K), and copper (Cu) contents were determined for ‘Clausellina’; phosphorus (P) for ‘Okitsu’; and sodium (Na) for ‘Silverhill’. Calcium (Ca), magnesium (Mg), iron (Fe), manganese (Mn), and zinc (Zn) uptake were similar for the mandarin cultivars. ‘Carrizo’ citrange at N, K, Mg, Mn, and Cu uptake; ‘Troyer’ citrange at N, P, K, and Fe uptake; and common sour orange at Ca, Zn, and Na uptake was superior on the other rootstocks. It was observed that ‘Carrizo’ and ‘Troyer’ citrange rootstocks had advantages over sour orange in nutrient uptake. Thus, growth performance, yield, and quality parameters considered, ‘Carrizo’ and/or ‘Troyer’ citranges could be suggested as rootstocks for the studied mandarin cultivars under similar ecological conditions.  相似文献   

Uniform ripening encodes a Golden 2-like transcription factor regulating tomato fruit chloroplast development     

Powell AL  Nguyen CV  Hill T  Cheng KL  Figueroa-Balderas R  Aktas H  Ashrafi H  Pons C  Fernández-Muñoz R  Vicente A  Lopez-Baltazar J  Barry CS  Liu Y  Chetelat R  Granell A  Van Deynze A  Giovannoni JJ  Bennett AB 《Science (New York, N.Y.)》2012,336(6089):1711-1715

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Effect of continuous 0.3 μL/L gaseous ozone exposure on fungicide residues on table grape berries     

Hakan Karaca  Spencer S. WalseJoseph L. Smilanick 《Postharvest Biology and Technology》2012,64(1):154-159

The persistence of residues of some fungicides, commonly applied in table grape vineyards to reduce bunch rot, was investigated during the cold storage of ‘Thompson Seedless’ table grape stemmed berries in atmospheres of air or 0.3 μL/L ozone enriched air. Grape berries were sprayed with a mixture of boscalid, iprodione, fenhexamid, cyprodinil, and pyrimethanil solutions, dried in air for 24 h, and packed in plastic clamshell containers in expanded polystyrene boxes. The boxes were stored either in ozone or in ambient air atmosphere (2 °C, 95% RH) for 36 d. Residue analyses were done initially and at 12-d intervals using gas chromatography-mass spectrometry. Residues of boscalid, iprodione, fenhexamid, and pyrimethanil declined during storage in air, but cyprodinil residues did not change significantly during 36-d storage. Storage in the ozone atmosphere markedly accelerated the rates of decline of fenhexamid, cyprodinil, and pyrimethanil, but not those of boscalid or iprodione. At the end of storage, degradation of fenhexamid, cyprodinil, or pyrimethanil was 1.6-, 2.8-, or 3.6-fold higher, respectively, in the ozone atmosphere compared that in air. Despite their structural similarity, pyrimethanil declined more rapidly in an ozone atmosphere than cyprodinil. Fenhexamid declined in both air and ozone more rapidly than the other fungicides; at the end of storage period, only 59.2% or 35.5% of the initial residue remained after air or ozone storage, respectively. Our results have shown that gaseous ozone treatment during storage has a great potential for degrading contemporary fungicides related to table grape production.  相似文献   

A parasitological, molecular and serological survey of Hepatozoon canis infection in dogs around the Aegean coast of Turkey     

Karagenc TI  Pasa S  Kirli G  Hosgor M  Bilgic HB  Ozon YH  Atasoy A  Eren H 《Veterinary parasitology》2006,135(2):113-119

Canine hepatozoonosis is caused by the tick-borne protozoon Hepatozoon spp. The prevalence of the infection in the Aegean coast of Turkey was investigated by examination of blood smear parasitology and polymerase chain reaction (PCR) using blood samples from 349 dogs collected from Central Aydin, Kusadasi, Selcuk, Central Manisa, Bodrum and Marmaris within the Aegean coast of Turkey. The indirect fluorescent antibody test (IFAT) for the detection of Hepatozoon canis antibodies was also used to detect the exposure rate to H. canis. PCR amplifying a 666bp fragment of 18S rRNA gene of Hepatozoon spp. was used in the epidemiological survey. The prevalence of Hepatozoon spp. infection was 10.6% by blood smear parasitology and 25.8% by PCR. IFAT revealed that 36.8% of serum samples were positive for antibodies reactive with Hepatozoon spp. The PCR products of 18S rRNA gene of Hepatozoon spp. isolated from six infected dogs, one isolate originating from each of the six different locations, were sequenced. The results of sequence analysis indicate that they are closely related to Indian and Japanese isolates of H. canis. This is the first epidemiological study on the prevalence of H. canis infection in the dog, in Turkey.  相似文献   

Lactobacillus spp. bacteria as probiotics in gilthead sea bream (Sparus aurata, L.) larvae: Effects on growth performance and digestive enzyme activities     

Cüneyt Suzer  Deniz oban  H. Okan Kamaci  ahin Saka  Kürat Firat   zge Otgucuolu  Hakan Küüksari 《Aquaculture (Amsterdam, Netherlands)》2008,280(1-4):140-145

In this study, the influence of commercial probiotic, Lactobacillus spp., supplementation was investigated on growth parameters and digestive enzyme activities in gilthead sea bream, Sparus aurata, during larval development. All experiments were triplicated and designed in three different administrations of probiotic from 3 days after hatching (DAH) concurrently with starting of exogenous feeding. In the first group, probiotic was added to live food (rotifer and Artemia). In the second group, probiotic was supplemented directly to both live food and water. In the third group, probiotic was added directly to water. Also, no probiotic treatment was maintained in control group. Total bacterial counts among probiotic probiotic-supplemented groups were significantly different from total bacterial counts in controls in water and digestive tract of larvae (p < 0.05). The mean of total bacterial counts in control was approximately 4 × 10⁴-fold increased from the experimental groups in the sea water (p < 0.05). Besides, mean digestive enzyme activities of all probiotics treatment groups were significantly different (p < 0.05) with that of the control. Except probiotic water supplementation group, in all treatments, the specific activities of pancreatic and intestinal enzymes were significantly higher (p < 0.05) in larvae to which probiotic had been supplemented by live food and live food with water. Also, S. aurata larvae that had probiotic administered by live food with water demonstrated significant (p < 0.05) increases in both survival (13–105% higher) and specific growth rate (2–9% higher) as compared to controls. As a result, supplementation of probiotic to directly tank water could not significantly increase growth parameters and digestive enzyme activities and therefore, administration of probiotics by this method would not be effective in terms of husbandry parameters and nutritional condition.  相似文献   

An Application of Landsat-5TM Image Data for Water Quality Mapping in Lake Beysehir, Turkey   总被引：1，自引：0，他引：1  

Bilgehan Nas  Semih Ekercin  Hakan Karabörk  Ali Berktay  David J. Mulla 《Water, air, and soil pollution》2010,212(1-4):183-197

The main goal of this study was to investigate spatial patterns in water quality in Lake Beysehir, which is the largest freshwater reservoir in Turkey, by using Landsat-5TM (Thematic Mapper) data and ground surveys. Suspended sediment (SS), turbidity, Secchi disk depth (SDD), and chlorophyll-a (chl-a) data were collected from 40 sampling stations in August, 2006. Spatial patterns in these parameters were estimated using bivariate and multiple regression (MR) techniques based on Landsat-5TM multispectral data and water quality sampling data. Single TM bands, band ratios, and combinations of TM bands were estimated and correlated with the measured water quality parameters. The best regression models showed that the measured and estimated values of water quality parameters were in good agreement (0.60 < R ² < 0.71). TM3 provided a significant relationship (R ²?=?0.67, p?<?0.0001) with SS concentration. MR between chl-a and various combinations of TM bands showed that TM1, TM2, and TM4 are strongly correlated with measured chl-a concentrations (R ²?=?0.60, p?<?0.0001). MR of turbidity showed that TM1, TM2, and TM3 explain 60% (p?<?0.0001) of the variance in turbidity. MR of SDD showed a strong relationship with measured SDD, with R ²?=?0.71 (p?<?0.0001) for the ratio TM1/TM3 and TM1 band combinations. The spatial distribution maps present apparent spatial variations of selected parameters for the study area covering the largest freshwater lake and drinking water reservoir in Turkey. Interpretation of thematic water quality maps indicated similar spatial distributions for SS, turbidity, and SDD. A large area in the middle portion of the lake showed very low chl-a concentrations as it is far from point and nonpoint sources of incoming nutrients. The trophic state index values were calculated from chl-a and SDD measurements. Lake Beysehir was classified as a mesotrophic or eutrophic lake according to chl-a or SDD parameters, respectively.  相似文献   

High cardiac troponin I plasma concentration in a calf with myocarditis     

Tolga Karapinar  Durrin Ozlem Dabak  Tuncay Kuloglu  Hakan Bulut 《The Canadian veterinary journal. La revue veterinaire canadienne》2010,51(4):397-399

A 15-day-old Brown Swiss calf, whose dam had suffered from foot-and-mouth disease, was presented with a history of depression and failure to suckle. The calf had an irregular cardiac rhythm and increased plasma cardiac troponin I (cTnI) detected with a commercial human immunoassay. The calf died the following day and myocarditis was detected. The cTnI assay may be useful in diagnosis of myocarditis in cattle.  相似文献